Viewing affirmative mentions of localization of IL2 (H. sapiens) in mononuclear cells

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Faist et al. (1993)IL-2mononuclear cellThe major regulatory level of interleukin-2 (IL-2) release under stressful conditions was determined via parallel analysis of IL-2 messenger RNA (mRNA) expression and IL-2 protein synthesis in mitogen-stimulated peripheral blood mononuclear cell (PBMCs) cultures on consecutive days postinjury.
Clouse et al. (1984)TCGFmononuclear cellsSecretion of TCGF is induced after stimulation of human peripheral blood mononuclear cells ( PBMNC ) with herpes simplex virus type 1 (HSV-1).
Lindqvist et al. (1987)interleukin-2PBLWe show in this study that phorbol esters alone can induce peripheral blood mononuclear cells (PBL) to secrete interleukin-2 (IL-2) and that the IL-2-dependent cell line CTLL can be used for measuring this lymphokine without influence of the phorbol esters themselves.
Deacock et al. (1992)interleukin-2mononuclear cellsA limiting dilution analysis (LDA) has been established which measures the total numbers of alloreactive interleukin-2 (IL-2)-secreting T cells in human peripheral blood mononuclear cells (PBMC).
Theobald et al. (1989)interleukin-2mononuclear cellsA limiting dilution (LD) culture was established which allows the detection of allospecific interleukin-2 (IL-2)-secreting helper T lymphocyte precursors (HTL-p) among human peripheral blood mononuclear cells (PBMNC).
Huang et al. (2002)interleukin-2mononuclear cells[Effects of sterigmatocystin on interleukin-2 secretion of human peripheral blood mononuclear cells in vitro].
Huang et al. (2002)interleukin-2mononuclear cellsThe effect of ST on interleukin-2 (IL-2) secretion of human peripheral blood mononuclear cells (HPBMc) in vitro was determined with enzyme-linked immunosorbent assay (ELISA) method to explore its putative effects on human immune function.
Venkataraman (1992)IL-2MNCsSimilar to freezing, L-leucine methyl ester (Leu-OMe) treatment (to eliminate IL-1 and prostaglandin E-2 (PGE-2)-secreting cells) also increased the IL-2-producing activities of fresh cells, but freezing no longer enhanced the production of IL-2 by Leu-OMe-treated cells, suggesting that (1) both the freezing process and Leu-OMe treatment have similar effects on IL-2 production, (2) the increased IL-2 secretion by frozen MNCs is independent of IL-1, and (3) inactivation of PGE-2-secreting cells during the freezing procedure is responsible for increased IL-2 secretion.
Vint et al. (1993)interleukin-2mononuclear cellsThe effects of auranofin on activation and interleukin-2 release from human peripheral blood mononuclear cells.
Vint et al. (1993)interleukin-2mononuclear cellsAuranofin, 30-300 nM causes a concentration-dependent potentiation of phytohaemagglutinin (PHA)-induced interleukin-2 (IL-2) release from human peripheral blood mononuclear cells in culture.
Kurahayashi et al. (1994)IL-2mononuclear cellsIt is considered that the NK activity of decidual mononuclear cells is suppressed in conditions of low IL-2 levels to permit the maintenance of pregnancy, but can be rapidly elicited by intrauterine infections or abortion, both of which elicit the secretion of IL-2.
Vega et al. (1999)IL-2mononuclear cellsIn order to understand the mechanism of this suppression, the effect of As was evaluated on the expression of CD25, and IL-2 secretion in human peripheral blood mononuclear cells (PBMC).
Fakhrai et al. (1997)IL-2PBMCThe levels of IL-2 secreted by transduced tumor cells and fibroblasts were evaluated by enzyme-linked immunosorbent assay (ELISA) of culture supernatants and compared with those of normal peripheral blood mononuclear cells (PBMC) activated in vitro with calcium ionophore and phorbol 12-myristate 13-acetate.
Fakhrai et al. (1997)IL-2PBMCThe highest levels of IL-2 secreted by transduced tumor cells (760 units/10(6) cells/24 h), adult fibroblasts (625 units/10(6) cells/24 h), and embryonic fibroblasts (3,975 units/10(6) cells/24 h) were 150- to 1,000-fold higher than than secreted by the activated PBMC (4 units/10(6) cells/24 h).
Flamand et al. (1995)IL-2PBMCIn fact, HHV-6-infected PBMC secreted 50% less IL-2 than mock-infected cells after mitogenic stimulation with OKT3 antibody or phytohemmaglutinin (PHA).
Hamai et al. (1998)interleukin-2mononuclear cellsSecretion of interleukin-2 from unstimulated peripheral blood mononuclear cells is a possible pathogenic mechanism in recurrent abortion.
Orosz et al. (1987)IL-2PBMCUsing this LDA technique, we have estimated that approximately 1/500 to 1/2000 (0.2% to 0.05%) of the PBMC from various individuals can secrete IL-2 after in vitro contact with completely major-histocompatibility-complex-disparate PBMC.
Faist et al. (1993)IL-2mononuclear cellsVia comparative analysis of mRNA expression and protein release the major regulatory levels of IL-1 and IL-2 release under stressful conditions were to be determined in mitogen respectively LPS stimulated PBMC (peripheral blood mononuclear cells) cultures on consecutive days (D) 1, 3, 5, 7 and 10 post injury.
Welte et al. (1987)IL-2PBMCIn contrast to our previous findings in recipients of unfractionated marrow, during weeks 3 to 7 post-SBA-E-BM transplantation (BMT), PBMC from the majority of patients spontaneously released IL-2 into the culture medium.
Rusiecka-Zió?kowska (2003)interleukin 2mononuclear cells[Secretion of interleukin 2, 6 and tumor necrosis factor by peripheral blood mononuclear cells in patients with uveitis induced by phytohemagglutinin or lipopolysaccharide].
Stein et al. (1996)IL-2PBMCThus, the concentration of TNF-alpha was significantly lower in the cell cultures from breast cancer patients than in controls, and patients with colorectal tumours released IFN-gamma/IL-2 (5%) in the supernatants significantly less frequently than PBMC from healthy controls (26%).
Mantovani et al. (1995)IL-2mononuclear cellsMoreover, we examined the ability of TAL and peripheral blood mononuclear cells (PBMC) to produce and release the cytokines and sIL-2R and to express membrane CD25 following their stimulation with phytohemagglutinin (PHA) in vitro.
Sriwanthana and Chavalittumrong (2001)IL-2mononuclear cellsFurthermore, the extract was shown to induce the IL-2 secretion from normal peripheral blood mononuclear cells (PBMC), whereas the IL-4 was not induced in the presence of the D. scandens extract.
Hassan and Reen (1996)IL-2MNCIn contrast to the secreted IL-2 levels, IL-2 mRNA expression was higher in antigen-stimulated neonatal MNC preparations, even though proliferation was lower.
Bloehr et al. (1992)interleukin 2PBMCT3 had no effect on the release of interleukin 2 by PBMC.
D'Ambrosio et al. (2004)IL-2PBMCThe immunomodulatory capacity of these compounds was evaluated by determining the interleukin-2 (IL-2) release in mitogen activated PBMC.
Lima and Vega (2005)interleukin-2mononuclear cellsMethyl-parathion and organophosphorous pesticide metabolites modify the activation status and interleukin-2 secretion of human peripheral blood mononuclear cells.
Ampel et al. (2005)IL-2mononuclear cellsMannan and anti-CD206 antibody significantly decreased the surface expression of mannose receptor (MR) on adherent peripheral blood mononuclear cells and reduced the interleukin-2 (IL-2) release induced by T27K.
Lotz et al. (1986)IL-2SFMCOKT3-induced proliferation and release of interleukin 1 (IL-1) and interleukin 2 (IL-2) from SFMC were depressed in all patients.
Komorowski et al. (1997)IL-2mononuclear cellsEffect of luteinizing hormone alpha-subunit on IL-2 and sIL-2R in vitro secretions from human peripheral blood mononuclear cells.
Komorowski et al. (1997)interleukin-2mononuclear cellsThe effect of luteinizing hormone alpha-subunit (LH alpha-SU) in concentrations of 50.0, 5.0, 0.5, and 0.05 mIU/ml on the release of interleukin-2 (IL-2) and soluble interleukin-2 receptor (sIL-2R) secretions in vitro from resting or phytohaemagglutinin (PHA) activated human peripheral blood mononuclear cells (PBMC) was studied.
Komorowski et al. (1997)IL-2PBMCLH alpha-SU in the concentrations tested did not influence the secretion of IL-2 (p > 0.05) or even depress the release of sIL-2R (50.0, 5.0, 0.5 mIU/ml; p < 0.001) into supernatants of PBMC cultures.
Dong et al. (2008)IL-2mononuclear cellSequential monitoring of T lymphocyte proliferation, IL-2 release, and the amount of HLA-DR on CD14+ mononuclear cell surface might be of clinical prognostic significance in patients with massive burn injury.
Gafter et al. (1994)interleukin-2mononuclear cellsThe in vitro secretion of interleukin-2 (IL-2), gamma-interferon (gamma-IFN), tumor necrosis factor (TNF) and colony stimulating factor (CSF) by human peripheral blood mononuclear cells isolated from patients and controls was used as a measure of immune function.
Wang et al. (1996)IL-2PBMCThe release of IL-2 and IL-6 by PHA-stimulated PBMC was significantly inhibited by titanium, chromium, and cobalt.
Murray et al. (1986)IL-2PBMCInterleukin-2 (IL-2) production following concanavalin A stimulation and the response of peripheral blood mononuclear cells (PBMC) to both IL-2 alone and IL-2 plus indomethacin, a prostaglandin synthetase inhibitor, were examined in 16 melanoma patients and 12 healthy controls.
Russwurm et al. (2002)IL-2PBMCMild hyperthermia significantly impaired IL-2 gene expression in PHA-stimulated cultures of PBMC and decreased IL-2 release in all variants of cultures.
Heaton et al. (1993)IL-2PBMCPolymerase chain reaction analysis of recombinant wild-type IL-2 or analogue-stimulated PBMC did not reveal the presence of IL-2 mRNA; thus, differential production of endogenous IL-2 could not account for these findings.
Oleksowicz et al. (1991)IL-2mononuclear cellsPlatelet secretion and inhibition of aggregation were an indirect consequence of a cellular effect of IL-2 on mononuclear cells, since aggregation was normal when whole blood was depleted of mononuclear cells and its reconstitution with autologous mononuclear cells led to a cell concentration-dependent inhibitory effect of aggregation and release of alpha-granule components in the presence of IL-2.
Sztein and Serrate (1989)interleukin-2mononuclear cellsThe present study focused on the characterization of the mechanisms involved in T alpha 1 and TF5 enhancement of phytohemagglutinin (PHA)-induced interleukin-2 (IL-2) secretion and interleukin-2 receptor (IL-2R) expression in human mononuclear cells.
Hercend et al. (1990)IL-2mononuclear cellsNatural killer lymphocytes (1-2 x 10(6] were purified from peripheral blood mononuclear cells and expanded during 4-5 weeks in the presence of IL-2 on microtiter plates containing feeder layers cells.
Moretti et al. (1992)interleukin-2mononuclear cellsCONCLUSION: Here we report a rapid and quantitative technique of limiting dilution analysis that can estimate the frequency of peripheral blood mononuclear cells capable of secreting interleukin-2 following interaction with specific alloantigen.
Siejka et al. (2005)interleukin-2mononuclear cells[Evaluation of the effect of GHRH(1-44)NH2 on the secretion of interleukin-2 (IL-2) and soluble IL-2 receptor alpha (sIL-2Ralpha) from human peripheral blood mononuclear cells in vitro].
Murray et al. (1985)IL-2Mononuclear cellsMononuclear cells from only one of 32 (3%) AIDS patients secreted normal levels of IL-2, and 21 (66%) failed to produce any detectable IL-2.
Lemire et al. (1985)IL 2PBMIn addition, 1,25-(OH)2-D3 produced a dramatic inhibition of interleukin 2 (IL 2) production by activated PBM, but did not inhibit IL 2 receptor generation by these cells.
Bernier et al. (1989)IL-2mononuclear cellAfter a 72-hr mononuclear cell stimulation, IL-2 release increases with PHA concentrations ranging from 0 to 10 micrograms/ml.
Warren et al. (1983)interleukin 2PBMCThe production and utilization of interleukin 2 (IL 2) by peripheral blood mononuclear cells (PBMC) from 14 bone marrow allograft recipients was examined.
Conti et al. (1992)IL-2PBMCThese studies have examined some biological properties of hrIL-1ra, such as its effects on the secretion of IL-1 alpha or IL-1 beta and IL-2, the surface expression of IL-2R and DNA synthesis by peripheral blood mononuclear cells (PBMC).
Conti et al. (1992)IL-2PBMCIn this report we found that hrIL-1ra inhibits, in a dose-dependent manner, the secretion of IL-1 alpha, IL-1 beta, IL-2, the surface expression of IL-2R and 3H-TdR incorporation in PBMC in vitro.
Schäfer et al. (1991)IL-2PMCWe conclude that PMC seem to retain their ability to migrate after IL-2 stimulation and 111In-labeling.
Alhomsi and Laposata (2006)IL-2mononuclear cellsRESULTS: Fatty acid ethyl esters inhibited the PHA-induced IL-2 production and secretion in activated human mononuclear cells.
Migliaccio et al. (1997)IL-2PBMCThat there is a correlation between the release of IL-2 and this DNase activity when using a complete PBMC population, B cell-depleted PBMC or macrophage-depleted PBMC stimulated with the peptides tested has been found.
Komorowski et al. (1997)IL-2mononuclear cellsEffects of hCG and beta-hCG on IL-2 and sIL-2R secretion from human peripheral blood mononuclear cells: a dose-response study in vitro.
Komorowski et al. (1997)IL-2mononuclear cellsThe effects of human chorionic gonadotropin (hCG) as well as beta-subunit of hCG (B-hCG) in concentrations of: 80,000/25,000; 500; 50; 5 mIU/ml on in vitro release of interleukin-2 (IL-2) and soluble interleukin-2 receptor (sIL-2R) from resting or phytohemagglutinin (PHA) activated human peripheral blood mononuclear cells (PBMC) were studied.
Komorowski et al. (1997)IL-2PBMCWe found that hCG in the dilutions of 80,000 mIU/ml (P < 0.01) and 5 mIU/ml (P < 0.05) diminished IL-2 secretion only from PHA activated PBMC. beta-hCG in concentrations of 5 mIU/ml (P < 0.05), 500 mIU/ml (P < 0.01) and 25,000 mIU/ml (P < 0.05) also diminished IL-2 secretion from PHA activated PBMC, and only in a dilution of 25,000 mIU/ml (P < 0.05) from resting PBMC.
Komorowski et al. (1997)IL-2PBMCSimultaneously, hCG in concentration of 80,000 mIU/ml (P < 0.01) potentiated the release of sIL-2R into supernatants from resting and PHA activated PBMC, but in concentration of 50 mIU/ml (P < 0.05) slightly depressed the secretion of IL-2 from PHA activated PBMC cultures. beta-hCG in dilution of 25,000 mIU/ml (P < 0.001) stimulated the release of sIL-2R from resting or PHA activated PBMC. beta-hCG had also inhibitory effect on sIL-2R secretion from resting (in a dilution of 50 mIU/ml; P < 0.05) and PHA activated (500 mIU/ml; P < 0.01) PBMC.
Komorowski et al. (1997)IL-2PBMCThe inhibitory effect of very high concentrations of hCG and beta-hCG on IL-2 secretion together with their stimulatory effect on sIL-2R release from PBMC may be an important event during the human pregnancy and various cancers.
Ihenetu et al. (2003)interleukin 2mononuclear cellsPharmacological characterisation of cannabinoid receptors inhibiting interleukin 2 release from human peripheral blood mononuclear cells.
Ihenetu et al. (2003)interleukin-2mononuclear cellsThe effects of a range of cannabinoid receptor agonists and antagonists on phytohaemagglutinin-induced secretion of interleukin-2 from human peripheral blood mononuclear cells were investigated.
Ihenetu et al. (2003)interleukin-2mononuclear cellsIn summary, WIN55,212-2 and JWH 015 inhibited interleukin-2 release from human peripheral blood mononuclear cells via the cannabinoid CB(2) receptor.
Avice et al. (2001)IL-2mononuclear cellsWe recently reported that CD47 ligation inhibited IL-2 release by umbilical cord blood mononuclear cells activated in the presence of IL-12, but not IL-4, preventing the induction of IL-12Rbeta(2) expression and the acquisition of Th1, but not the Th2 phenotype.
Bailey et al. (2002)IL-2mononuclear cellsA multidonor ELISPOT study of IL-1 beta, IL-2, IL-4, IL-6, IL-13, IFN-gamma and TNF-alpha release by cryopreserved human peripheral blood mononuclear cells.
Krummel et al. (2010)IL-2mononuclear cellsand separately for IL-2 release by mononuclear cells were performed using test-plates from AID, Strassberg, Germany.
Mullen et al. (2007)IL-2PBMCCLA supplementation significantly reduced Con A-stimulated PBMC IL-2 secretion (37.1%; P=.02).
Mullen et al. (2007)IL-2PBMCThis study showed that CLA supplementation reduced PBMC IL-2 secretion from Con A-stimulated PBMC but lacked effect on other markers of the human inflammatory response.
Derzic et al. (2005)IL-2 CBMNCMETHODS: The aim of our study was to compare the anti-tumor potential of CB mononuclear cells (MNC), matured in the presence of IL-2, to BM, and to determine phenotype and cytokine secretion in IL-2 CB MNC culture during the peak of their anti-leukemia cytotoxic activity.
Theobald et al. (1990)IL-2-secretingmononuclear cellsLimiting dilution cultures were performed to detect allospecific IL-2-secreting helper T lymphocyte precursors (HTL-p) among human peripheral blood mononuclear cells, E-rosette-purified (E+) and cell-sorter-separated CD4+/8- as well as CD4-/8+ T cell subsets.
Le et al. (1986)interleukin 2PBMCAddition of exogenous interleukin 2 (IL 2) markedly enhanced IFN-gamma secretion by PBMC induced with LPS.
Watzl et al. (2003)IL-2mononuclear cellsImmune status was assessed by measuring lytic activity of natural killer (NK) cells, secretion of cytokines (IL-2, IL-4, TNFalpha), and proliferation by activated peripheral blood mononuclear cells.
Breen et al. (2000)IL-2PBMCThe use of fresh or frozen cells gave comparable cytokine mRNA data; however, the secretion of some cytokine proteins (IL-2 receptor, IL-10, and TNF-alpha) appeared to be reduced in HIV+ PBMC that had been frozen and thawed.
Blasco et al. (1995)IL-2PBMCJacalin-CD4 interaction and the proliferation of PBMC, as well as IL-2 secretion by Jurkat cells were inhibited by specific jacalin-competitive sugars.
Kelley et al. (2001)IL-2PBMCCLA supplementation did not alter the in vitro secretion of prostaglandin E2, leukotriene B4, interleukin-1beta (IL-1beta), or tumor necrosis factor alpha (TNFalpha) by PBMC simulated with lipopolysaccharide, and the secretion of IL-2 by PBMC stimulated with phytohemagglutinin.
Ahne and Mayr (2000)IL-2mononuclear cellsThe reptilian paramyxoviruses FDLV and GOV initiated the production and release of cytokines like IL-1alpha, IL-1beta, IL-2, TNF-alpha and IFN-alpha in human peripheral blood mononuclear cells (PBMC) at 37 degrees C.
Kapp et al. (1991)IL-2mononuclear cellsIn comparison with psoriasis patients and normal controls, PHA-stimulated mononuclear cells of atopic dermatitis patients released significantly less IL-2 into supernatants.
Kapp et al. (1991)IL-2mononuclear cellsAtopic dermatitis, however, is further characterized by the decreased capacity of mononuclear cells to release IL-2 upon stimulation in vitro.