Viewing affirmative mentions of gene expression of Foxp3 (M. musculus) in T cells

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Wan and Flavell (2007)Foxp3T cellsThe immune-suppressive activities of T cells with attenuated Foxp3 expression were nearly abolished in vitro and in vivo, whereas their anergic properties in vitro were maintained.
Chang et al. (2008)FoxP3T cellRecent studies by several groups have demonstrated that FoxP3 is expressed outside T cell lineages.
Torgerson and Ochs (2002)FOXP3 proteinT cellsRecent data have demonstrated that this process requires antigenic stimulation and that the degree to which the immune system responds is inversely proportional to the level of FOXP3 protein (Forkhead box P3) expression in peripheral T cells.
Duarte et al. (2009)Foxp3T cellsInduction of Forkhead-box p3 (Foxp3) expression in developing T cells upon peptide-MHC encountering has been proposed to define a lineage of committed Treg cells.
Ohata et al. (2007)Foxp3T cellOBJECTIVE: To investigate the efficacy of type II collagen-reactive Foxp3-expressing T cell transfer in suppressing collagen-induced arthritis (CIA) in relation to disease progression.
Ohata et al. (2007)Foxp3T cellsAn elevated expression level of Foxp3 in type II collagen-specific T cells improved their suppressive function in CIA.
Ohata et al. (2007)Foxp3T cellsThus, transfer of T cells expressing high levels of Foxp3 could be a strategy to overcome the induced resistance to regulatory T cell therapy.
Selvaraj and Geiger (2007)Foxp3T cellsTGF-beta induces Foxp3 expression in stimulated T cells.
Selvaraj and Geiger (2007)Foxp3T cellsThus, Foxp3 expression in naïve-stimulated T cells is transient in vitro, dependent on TGF-beta activity within a highly restricted window after activation and continuous TGF-beta presence.
Wan and Flavell (2005)Foxp3T cellsIn addition, we showed that de novo Foxp3 expression along with suppressive function were induced by TGF-beta in activated CD4 T cells in vitro.
Wan and Flavell (2005)Foxp3T cellsFinally, we demonstrated that non-Foxp3-expressing CD4 T cells could not be converted into Foxp3-expressing cells upon adoptive transfer into immunodeficient hosts.
Selvaraj and Geiger (2007)Foxp3T cellsTGF-beta induces Foxp3 expression in stimulated T cells.
Selvaraj and Geiger (2007)Foxp3T cellsThus, Foxp3 expression in naïve-stimulated T cells is transient in vitro, dependent on TGF-beta activity within a highly restricted window after activation and continuous TGF-beta presence.
Gavin et al. (2007)Foxp3T-cellIt has been suggested that Foxp3 expression is required for both survival of Tr precursors as well as their inability to produce interleukin (IL)-2 and independently proliferate after T-cell-receptor engagement, raising the possibility that such 'anergy' and Tr suppressive capacity are intimately linked.
Fontenot et al. (2005)Foxp3T cellsWe show that expression of Foxp3 is highly restricted to the subset alphabeta of T cells and, irrespective of CD25 expression, correlates with suppressor activity.
Fontenot et al. (2005)Foxp3T cellsInduction of Foxp3 expression in nonregulatory T cells does not occur during pathogen-driven immune responses, and Foxp3 deficiency does not impact the functional responses of nonregulatory T cells.
Schallenberg et al. (2010)Foxp3GFPT cellsFurthermore, CD4+CD25+ T cells with in vitro up-regulated Foxp3GFP protein expression exhibited mRNA expression of so-called T reg signature genes such as CD103, CTLA-4, Gpr83, and Helios (Fig. 5 B and not depicted).
Schallenberg et al. (2010)Foxp3T cellsR–mediated up-regulation of Foxp3 expression in TCR-stimulated conventional CD4+ T cells in vitro (Fig.
Schallenberg et al. (2010)Foxp3T cellsprecursor populations from polyclonal nonmanipulated mice and TCR transgenic T cells during DC-targeted T reg cell conversion revealed striking similarities with regard to activation marker phenotype and requirements to up-regulate Foxp3 expression.
Schallenberg et al. (2010)Foxp3T cellsThis observation, together with the notion that splenic CD8+DEC-205+ DCs induce Foxp3 expression in vitro in initially CD4+Foxp3– T cells without added TGF-?
Schallenberg et al. (2010)Foxp3T cellsIn addition, our experiments on DC-targeted T reg cell conversion of TCR transgenic CD4+ T cells indicated that both foreign and self-antigens can efficiently induce Foxp3 expression.
Mor et al. (2008)Foxp3T cellExcept for H-Ras transduction with shRNA, each inhibitory mode increased expression of Foxp3 and nuclear factor of activated T cell proteins, and surface expression of CD25.
Floess et al. (2007)Foxp3T cellsTo prove experimentally the stability of Foxp3 expression in natural Tregs on a cellular level, we adoptively transferred sorted CD25+CD4+ T cells after CFSE (carboxy fluorescein diacetate succinimide ester) labeling into syngeneic recipients.
Floess et al. (2007)FOXP3T cellsTemporary expression of FOXP3 can be detected in activated T cells lacking regulatory function, especially in the human system [28,29].
Osorio et al. (2008)Foxp3T-cellHere, we show that mouse CD25(+)Foxp3(+) Treg can be converted into a hybrid T-cell population characterized by the expression of Foxp3 and ROR-gammat and the production of IL-17.
Osorio et al. (2008)Foxp3T cellsThese results indicate that some Foxp3(+) T cells can produce IL-17 while retaining Foxp3 expression and suggest that Treg could play an unexpected pro-inflammatory role in some settings.
Dujardin et al. (2004)Foxp3T cellsRegulatory potential and control of Foxp3 expression in newborn CD4+ T cells.
Dujardin et al. (2004)Foxp3 mRNAT cellIn adult day 3-thymectomized mice, the CD25(+)CD4(+) T cell subset is overrepresented (most of the cells being CD103(+)) and expresses high amounts of Foxp3 mRNA, independent of the development of autoimmune gastritis.
Wang et al. (2007)Foxp3T cellsFoxp3-expressing CD4(+)T cells under the control of INF-gamma promoter prevent diabetes in NOD mice.
Barnes et al. (2009)Foxp3T cellRegulatory T (T(reg)) cells expressing forkhead box P3 (Foxp3) arise during thymic selection among thymocytes with modestly self-reactive T cell receptors.
Carson and Ziegler (2007)Foxp3T cellsWe have used a Foxp3 transgenic mouse line to demonstrate that Foxp3 expression correlates with attenuated TCR signaling, and that the deficit in Foxp3-transgenic CD4 T cells, as well as in CD4(+)CD25(+) Tregs, affects multiple biochemical pathways.
Merkenschlager and von Boehmer (2010)Foxp3T cellPI3 kinase signalling blocks Foxp3 expression by sequestering Foxo factors Expression of the regulatory T (T reg) cell–associated transcription factor Foxp3 can be induced by signals from the T cell receptor (TCR), interleukin-2 (IL-2), and transforming growth factor (TGF)-?.
Merkenschlager and von Boehmer (2010)Foxp3T cellsMajor inducers of Foxp3 expression in peripheral T cells in vitro include TCR signaling in the presence of TGF-?
Merkenschlager and von Boehmer (2010)Foxp3T cellsIn agreement with previous work (Wohlfert et al., 2006), Harada et al. (2010) found that Cbl-b–deficient CD4 T cells expressed less Foxp3 in response to TGF-?
Kasprowicz et al. (2005)FoxP3T cellDynamic regulation of FoxP3 expression controls the balance between CD4+ T cell activation and cell death.
Kasprowicz et al. (2005)FoxP3T cellWhile the overall phenotypic effects of FoxP3 expression are evident, the mechanism by which FoxP3 regulates T cell activation is not well understood.
Kasprowicz et al. (2005)FoxP3T cellsCD4+ T cells from mice that express a FoxP3 Tg are refractory to TCR-mediated stimulation, failing to proliferate or produce cytokines, but possess suppressive activity towards normal T cells.
Yang et al. (2007)Foxp3T cellsCD70+ non-Hodgkin lymphoma B cells induce Foxp3 expression and regulatory function in intratumoral CD4+CD25 T cells.
Yang et al. (2007)Foxp3T cellsHowever, recent studies suggest that forkhead box P3 (Foxp3) is expressed in CD4(+)CD25(-) T cells in aged mice.
Yang et al. (2007)Foxp3T cellsIn the present study in B-cell non-Hodgkin lymphoma (NHL), we found that a subset of intratumoral but not peripheral blood CD4(+)CD25(-) T cells, comprising about 15% of intratumoral CD4(+) T cells, express Foxp3 and are capable of suppressing the proliferation of autologous infiltrating CD8(+) T cells.
Yang et al. (2007)Foxp3T cellsIn vitro activation with OKT3/anti-CD28 antibody (Ab) or dendritic cells (DCs) induced Foxp3 expression in a subset of these CD4(+)CD25(-)Foxp3(-) T cells.
Barnes et al. (2009)Foxp3T cellsTo find the causative mutation, we mapped the king phenotype by outcrossing the king stock (C57BL/6J background) to C3H/HeN mice, backcrossing to the king stock, and measuring the percentage of circulating CD4+ T cells expressing Foxp3 in the blood of F2 mice (Figure 2A).
Barnes et al. (2009)Foxp3T cellsare sufficient to induce Foxp3 in Carma1k/k CD4+ T cells, we explored the possibility that these cytokines activated signaling pathways downstream of CARMA1, or induced Foxp3 expression via an alternative pathway.
Popmihajlov et al. (2008)FOXP3T cellFOXP3 expression normally functions to down-regulate T cell activation, particularly cytokine gene expression.
Popmihajlov et al. (2008)FOXP3T cellsGiven the expression of FOXP3 by TCR/IL2-activated T cells, what can be expected as to the outcome of the expression of this gene?
Popmihajlov et al. (2008)FOXP3T cell-facilitated induction of anti-CD3 activation of murine T cell FOXP3 expression[47].
Popmihajlov et al. (2008)FOXP3T cellsAs shown in Fig 1D, the mean FOXP3 expression of freshly isolated T cells is compared with the mean FOXP3 expression after 24 hours of culture with anti-CD3.
Popmihajlov et al. (2008)FOXP3T cellsThus, even though 86.4% (15.9% +70.5%) of the CD4+ T cells became CD25+ after 24 hours, only 18.4% of the CD4+CD25+ cells also expressed FOXP3.
Popmihajlov et al. (2008)FOXP3T cellsRepresentative 4-quadrant contour plots of T cells from PBMCs cultured with anti-CD3 for 24 hours are shown in Fig 1C, comparing FOXP3 expression and CD25 expression.
Popmihajlov et al. (2008)FOXP3T cellsIt is readily apparent that CD25 expression precedes FOXP3 expression and eventually is detectable on >80% of both CD4+ and CD8+ T cells by 48 hours following activation, thereby attesting to the efficiency of anti-CD3 activation.
Popmihajlov et al. (2008)FOXP3T cellsVery recently, others reported that antibodies reactive with IL2 or the IL2 receptor diminished TCR/CD3-induced FOXP3 expression by CD8+ T cells [34].
Popmihajlov et al. (2008)FOXP3T cellsOf interest, it was found that peak expression of FOXP3 in human T cells requires as long as three days after activation with anti-CD3 in vitro, thereby almost excluding an immediate/early FOXP3 gene activation via the TCR/CD3 complex [33].
Popmihajlov et al. (2008)FOXP3T cellThese findings prompted the speculation that the expression of FOXP3 in an antigen-activated T cell could act as a natural negative feedback loop that would prevent unrestricted cytokine production and inflammatory reactions [31].
Popmihajlov et al. (2008)FOXP3T cellsAccordingly, FOXP3 expression by CD4+CD25+ T cells was quickly adopted as a more definitive phenotypic definition of a T-Reg cell.
Guo et al. (2010)Foxp3T-cellInhibition of clonal expansion by Foxp3 expression as a mechanism of controlled T-cell responses and autoimmune disease.
Jiang et al. (2007)Foxp3T cellsIncreased expression of Foxp3 in splenic CD8+ T cells from mice with anterior chamber-associated immune deviation.
Jiang et al. (2007)Foxp3T cellsCONCLUSIONS: An upregulated Foxp3 expression in CD8+ T cells is associated with the development of ACAID, suggesting an involvement of CD8+ Foxp3+ T cells in this model of immune tolerance.
Corthay (2009)Foxp3T cellsHowever, it is now well documented that most human CD4+ and CD8+ T cells transiently express Foxp3 upon activation [53, 61–65].
Corthay (2009)Foxp3T cellsThe expression of Foxp3 is mostly restricted to CD4+ T cells, but some CD8+ T cells do express Foxp3 as well [60].
Jaeckel et al. (2005)Foxp3T-cellsHere we have explored the fact that ectopic expression of the transcription factor Foxp3 can confer a suppressor phenotype to naive CD4(+) T-cells.
Jiang et al. (2007)Foxp3 proteinT cellsThe expression of Foxp3 protein in the splenic CD8+ T cells in ACAID was increased after stimulation with anti-CD3 plus anti-CD28 mAb or specific antigen in vitro.
Jiang et al. (2007)Foxp3T cellsSince Foxp3 has been generally considered to be a specific marker for Tregs both in rodents and humans in spite of CD4 or CD8 lineage [14], a study was performed to examine the expression of Foxp3 in CD8+ T cells at both the mRNA level and protein level during ACAID.
Jiang et al. (2007)Foxp3T cellsThe results showed that an upregulated expression of Foxp3 in CD8+ T cells could only be shown at both the mRNA level and protein level when cells were stimulated using polyclonal activation.
Jiang et al. (2007)Foxp3T cellsThe result showed that there was no difference between stimulated and unstimulated PBS-AC-injected control mice and normal mice concerning the expression of Foxp3 in CD8+ T cells.
Jiang et al. (2007)Foxp3T cellsSasaki and colleagues [34] found that the expression of Foxp3 was almost 88% in CD8+ T cells from such transgenic mice when these T cells were exposed to TGF-?
Jiang et al. (2007)Foxp3T cellSince both CD4+ and CD8+ T cells expressing Foxp3 play a role in ACAID, it is interesting to find out which Foxp3+ T cell population is indispensable or whether both populations are needed for the development of ACAID.
Jiang et al. (2007)Foxp3T cellsIn conclusion, our study revealed an inhibitory effect of CD8+ T cells on the expression of the DTH response and an increased expression of Foxp3+ on CD8+ T cells in an OVA-induced ACAID model.
Osorio et al. (2008)Foxp3T cellsWe confirmed that the majority of sorted CD25+FR4+ CD4+ T cells express Foxp3 (Fig. 3A).
Osorio et al. (2008)Foxp3T cellsThe majority of these cells coexpressed Foxp3 (Fig. 3B) as observed in experiments with DEREG T cells (see above).
Osorio et al. (2008)Foxp3T cellsIn those studies, Foxp3+ T cells may pass through a transient stage characterized by the coexpression of Foxp3 and IL-17 before downregulating Foxp3.
Harada et al. (2010)Foxp3T cellsTo determine whether Foxo3a acts as a downstream target of Cbl-b, we examined whether Foxo3a can rescue the defective Foxp3 expression in Cbl-b–deficient T cells.
Harada et al. (2010)Foxp3T cellsRetroviral transduction of wild-type Foxo3a increased the Foxp3 expression in Cbl-b–deficient T cells to a certain degree (Fig. 4 A, bottom).
Harada et al. (2010)Foxp3T cellsNotably, the constitutively active Foxo3a mutant augmented Foxp3 expression to the same level in wild-type and Cbl-b–deficient T cells.
Harada et al. (2010)Foxp3T cellsAblation of Foxo3a expression resulted in a marked reduction of Foxp3 expression in iT reg cells (Fig. 4 C), similar to that observed in Cbl-b–deficient T cells.
Harada et al. (2010)Foxp3T cellsRe-introduction of wild-type Foxo3a largely restored the Foxp3 expression in Foxo3a-deficient T cells to a degree similar to wild-type control cells (Fig. 4, D and E).
Harada et al. (2010)Foxp3T cellsHowever, detailed examination of the mTOR-deficient T cells showed that only the mTOR complex 2 (TORC2), which is less sensitive to rapamycin, but not the rapamycin-sensitive TORC1, is involved in Foxp3 expression (Delgoffe et al., 2009).
Kelsen et al. (2005)FoxP3T cellsWe investigated the expression of FoxP3 and regulatory potential of gut-derived CD4(+)CD25(+) T cells cultured from patients with CD and healthy individuals.
Kelsen et al. (2005)FoxP3T cellsThe FoxP3 expression was analysed by reverse transcriptase polymerase chain reaction (RT-PCR), and the suppressive effect of FoxP3(+)CD4(+)CD25(+) T cells on proliferation and cytokine production of autologous CD4(+) T cells was assessed by flow cytometry.
Josefowicz et al. (2009)Foxp3T cellIn addition, CD4-Cre x dnmt1(fl/fl) mice harbored sizeable thymic and peripheral populations of CD8(+)Foxp3(+) cells, suggesting that Dnmt1 activity is required for restricting Foxp3 expression to the CD4 T cell lineage.
Josefowicz et al. (2009)Foxp3T cellsIn the absence of Dnmt1, Foxp3 induction in thymic and peripheral Foxp3-negative T cells was maximized upon TCR engagement, and the provision of TGF-beta was dispensable for Foxp3 expression.
Liston et al. (2008)Foxp3T cellThe high level of Foxp3 expression, a hallmark of T reg cells, is needed to establish and maintain a distinct transcriptional program determining metabolic, signaling, and effector features (i.e., suppressive functions), distinguishing these cells from other T cell lineages (10–14).
Sedwick (2008)Foxp3T cellsTherefore, one experimental strategy to treat autoimmune diseases involves extracting bulk populations of T cells, modifying them by forcing Foxp3 expression, and then readministering them to the animal—where they should, in theory, suppress disease progression.
Sedwick (2008)Foxp3T cellsIt's possible that forcing Foxp3 expression changes the patterns of expression of other homing receptors on T cells; if this is the case, the cells may be forced to go to the liver, unable to provide systemic relief.
Parietti et al. (2008)forkhead box P3T cellsRESULTS: MRL/lpr mice exhibited a normal percentage of CD4+,CD25 high T cells, and forkhead box P3 messenger RNA and protein expression in Treg cells was not altered.
Li et al. (2006)Foxp3T cellsDevelopment of CD8+ T cells and NKT cells, maintenance of peripheral Foxp3-expressing regulatory T cells, and survival of CD4+ T cells all depended on TGF-beta signaling.
Toes et al. (2004)Foxp3T-cellMoreover, CD4+ T-cell lines directed against HC Gp39 expressed CD25, GITR, CTLA-4 and Foxp3 molecules and were capable of suppressing other immune responses.
Lu et al. (2007)Foxp3T cellsRecently, it has been suggested that GPR83 is involved in the induction of Foxp3 expression in the peripheral nonregulatory Foxp3- CD4 T cells.
Lu et al. (2007)Foxp3T cellsFurthermore, while in vitro analysis suggested a potential involvement of GPR83 in transforming growth factor beta-dependent Foxp3 induction, there was no difference in the ability of nonregulatory GPR83-deficient and nondeficient Foxp3- T cells to acquire Foxp3 expression in vivo.
Chong et al. (2008)Foxp3T cellsFoxp3 expression can also be induced in naive CD4+ T cells upon antigen stimulation in the presence of TGF-?
Chong et al. (2008)Foxp3T cellsCD4-cre mice to up-regulate Foxp3 expression could be caused by generation of abnormal T cells that had been aberrantly selected in the absence of Drosha.
Izcue et al. (2008)Foxp3T cellsIL-23 has been suggested to act directly on T cells to inhibit Foxp3 expression (Zhou et al., 2007).
Hsu et al. (2006)forkhead box P3T cellsExcept for the high expression level of interleukin (IL)-10 mRNA, CD4(+)CD25(+) T cells from lupus mice expressed normal forkhead box P3 (Foxp3) and transforming growth factor (TGF)-beta mRNA, and exerted suppressive functions.
Venuprasad et al. (2008)Foxp3T cellsHere we show that Itch-/- T cells were resistant to TGF-beta treatment and had less Foxp3 expression.
Fontenot et al. (2003)Foxp3T cellsFurthermore, ectopic expression of Foxp3 confers suppressor function on peripheral CD4+CD25- T cells.
Jiang et al. (2006)FoxP3T cellsAs shown in Fig. 3, adult Treg cells expressing FoxP3 showed no proliferation and efficiently suppressed responder T cells.
Jiang et al. (2006)FoxP3T cellsIn addition, TSLP failed to induce FoxP3 expression in CD4+CD25- T cells from the spleen under similar culture conditions (Fig. 10).