Viewing negative mentions of gene expression of Il4 (M. musculus) in T cells

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Inoue et al. (1999)IL-4T-cellsIL-4 was not detected in the serum of CD4+ T-cells-depleted mice.
Karulin et al. (2002)IL-4T cellsThis IL-4 was not produced by T cells, but soluble factors secreted by the recall Ag-activated T cells, including IL-3, triggered cells of the innate immune system, primarily mast cells, to secrete IL-4.
Erb et al. (1999)IL-4T cellsPurified CD8+ T cells from uninfected or flu infected IL-4 transgenic (tg) animals produced no detectable IL-4 or IL-5 after in vitro stimulation on anti-CD3 coated plates.
Chen et al. (1997)IL-4T cellsHowever, splenic T cells from SM/J and B10.SM (H-2v, neu-1a) strain mice, deficient in neu-1 sialidase activity, failed to produce IL-4 but produced normal levels of IL-2 following activation.
Rodriguez et al. (2009)IL-4T-cellsIL-4 was not detected in any sample, but IL-13 levels were also comparable between normal and T-cell-deficient mice indicating Th2-polarized T-cells are not the sole source of this cytokine.
Suzuki et al. (1992)IL-4T cellsThese results suggest that the allo-CTL induction in the primary response is IL:-2-dependent and secondary allo-CTL induction is both IL-2 and IL-4-dependent, because unprimed CD4+ T cells produce IL-2, but not IL-4, whereas primed cells produce both IL-2 and IL-4 in response to alloantigens.
Tkaczyk et al. (2000)IL-4T-cellRESULTS: We report that addition of IL-4-treated BMMCs to normal spleen cells resulted within 48 hours in a B- and T-cell activation with substantial amounts of the T(H1) cytokines IFN-gamma and IL-12 and no detectable IL-4.
Fachado et al. (2003)IL-4T-cellThis immunization resulted in a Th1-type response with predominance of IgG2a and a specific T-cell proliferation with high levels of interferon-gamma (IFN-gamma) secretion, whereas no IL-4 was detected.
Dieli et al. (1998)IL-4T cellsEarly IL-4 production was required for the development of IL-4-producing CD4+ T cells as mice injected with anti-V(alpha)14 or anti-IL-4 mAb produced little IL-4 and IL-10, while production of IFN-gamma was increased approximately 2-fold.
Gollob and Coffman (1994)IL-4T cellsIn contrast, CD4+ T cells stimulated with immobilized anti-V beta 6 under otherwise identical culture conditions generated cells that produced IFN-gamma but not IL-4 on restimulation.
Macaulay et al. (1998)IL-4T cellsAlthough T cells primed in the absence of B cells could proliferate in response to Ag presented by B cells and could induce Ag-specific IgM production, such T cells failed to produce high levels of IL-4 as are normally induced in T cells by Ag-presenting B cells.
Panoutsakopoulou et al. (1998)IL-4T cellsOn the other hand, C57BL mice do not produce IL-4 and, in the absence of both CD8+ T cells and IFN-gamma, still generate an effective anti-virus immune response.
Mike et al. (1999)IL-4T cellsHowever, LC injection affected neither expression of interferon-gamma (IFN-gamma) and IL-4 mRNAs nor proliferative capacities of splenic T cells.
Derecki et al. (2010)IL-4T cellsT cells expressed no IL-4 and high levels of IFN-?
Röcken et al. (1991)IL-4T cellsThe factors responsible for the development of CD4+ T cells which produce IL-2 but not IL-4 and cells capable of producing IL-4 but not IL-2 are unknown.
Venkataraman and Kuo (2005)IL-4T cellsInterestingly, primary stimulation of T cells with anti-CD3 resulted in increased IL-4 but not IL-2 or IFN-gamma production in GPR84(-/-) mice compared to wild-type mice.
Mosmann et al. (1987)IL 4T cellsIn contrast, the T cell stimulating activities of mouse and human B cell stimulatory factor 1 (interleukin 4; IL 4) appear to be species specific over the range of concentrations tested; we detected no activity of mouse IL 4 on human T cells, or human IL 4 on mouse T cells.
Yamane et al. (2005)IL-4T cellsIn addition, naive CD4+ T cells from GATA-3 conditional KO mice failed to produce early IL-4 in response to TCR/CD28 stimulation.
Hossain et al. (2000)IL-4T cellsStimulation of peritoneal DN TCRalphabeta+ T cells with plate-bound anti-TCRbeta monoclonal antibodies showed proliferation and also produced IFN-gamma but not IL-4.
Lindell et al. (2008)IL-4T cellsAs expected, CD4+ T cells alone (>99.5% pure) produced no detectable IL-4, IL-5, IL-13, IL-17 or IFN?
Kurowska-Stolarska et al. (2008)IL-4T cellsWe report here that the new cytokine IL-33, in the presence of Ag, polarizes murine and human naive CD4(+) T cells into a population of T cells which produce mainly IL-5 but not IL-4.
Cao et al. (1993)IL-4T cellFive of these T cell clones produced both IL-2 and IFN-gamma but not IL-4 after stimulation with either phorbol 12-myristate 13-acetate (PMA) or concanavalin A (Con A).
Cao et al. (1993)IL-4 mRNAT cellAll the T cell clones tested which secreted INF-gamma and LT expressed no measurable IL-4 mRNA.
Mendel and Shevach (2002)IL-4T cellsPrevious studies have suggested that activation of CD4+ T cells in the presence of IL-10 results in the generation of a population of T cells, T regulatory 1 (Tr1) cells, that primarily produce IL-10 and TGF-beta, but not IL-4.
Xu et al. (1997)IL-4T cellA long-term uveitogenic T cell line, initially derived in the presence of IL-12, produced IFN-gamma and IL-2, but not IL-4, and was CD4+ (Th1-like).
Smythies et al. (2000)IL-4T cellsSplenic T cells from the same infected WT mice produced high levels of IFN-gamma, no detectable IL-4, and low amounts of IL-10 following in vitro H. pylori urease stimulation, reflecting a systemic Th1 response.
Wang and Mosmann (2001)IL-4T cellsIn vivo priming of CD4 T cells that produce interleukin (IL)-2 but not IL-4 or interferon (IFN)-gamma, and can subsequently differentiate into IL-4- or IFN-gamma-secreting cells.
Matesic et al. (1998)IL-4T cellsAdoptive transfer of the IL-4/IL-5-producing CD4+ T cells, but not the CD8+ T cells, induced a distinct histopathology characterized by marked eosinophilic infiltration of the skin.
Liu et al. (1991)IL-4T cellsSubsequent antibody blocking experiments employing the monoclonal anti-murine IL-4 antibody 11B11 revealed that BALB/c GLA-reactive 1 degree LN T cells and DL.4G6.1 did not produce detectable levels of IL-4 in their culture fluids when stimulated by GLA, which suggested that these cells, unlike DCL-2, were TH1-like in nature.
Boppana et al. (2009)IL-4T cellsSimilar to tick feeding (Figure 2), male and female SGE programmed adoptively transferred TCR transgenic CD4+ T cells to express IL-4 (P < 0.05) but not IFN-?
Lyadova et al. (1998)IL-4T-cellHowever, there was a clear difference between T-cell clones with respect to cytokine (gamma interferon [IFN-gamma] and interleukin-4 [IL-4] and IL-10) profiles: besides one Th1-like (IFN-gamma+ IL-4(-)) clone and one Th0-like (IFN-gamma+ IL-4(+) IL-10(+)) clone, three clones produced predominantly IL-10, with only marginal or no IL-4 and IFN-gamma responses.
Chowdhury et al. (2002)IL-4T cellsIn contrast, T cells from anti-ICAM-1/LFA-1-treated mice expressed IFN-gamma, IL-10 and TGF-beta1 but not IL-4.
Williams et al. (1992)IL-4T cellsIL-4 was not detected in supernatants from cultures of non-immune T cells in the presence of APC from any tissue.
Huber et al. (2001)IL-4T cellsVgamma4 and Vgamma1(+) T cells from Stat4ko mice expressed IL-4 but no or minimal IFN-gamma, whereas these cell populations derived from Stat6ko mice expressed IFN-gamma but no IL-4.
Li et al. (1998)IL-4T cellHence, a T cell dependent allograft rejection enabled by rapamycin-sensitive signals or signals mediated by binding of the gamma c chain occurs in the absence of both IL-2 and IL-4.
Bruhn et al. (1993)IL-4T-cellFurthermore, a CS1 multimer could drive a heterologous promoter in an IL-4-producing [helper T-cell type 2 (TH2-type)] T-cell clone but not in a non-IL-4-producing (TH1-type) clone, suggesting a mechanism by which IL-4 production could be differentially regulated in TH subsets.
Kobayashi et al. (1999)IL-4T cellsHowever, IL-4 was not produced by splenic T cells that were prepared from the same infected mice treated with IL-12 and sIL-4R in combination.
Miller et al. (1999)IL-4T cellsRecombinant-activating gene 2 (RAG-2-/-) T cell receptor-transgenic mice repeatedly injected with the superantigen staphylococcal enterotoxin A entered a tolerant state in which splenic CD4+ T cells produced little interleukin (IL)-2, interferon gamma, or IL-4.
Mori et al. (1994)IL-4T cellsWe in this study demonstrated that double negative (DN) T cells, the major cell population in lpr mice, failed to express interleukin-3 (IL-3), IL-4, IL-5, and IL-6 genes that influence B cell growth and activation.
Elewaut et al. (2000)IL-4T cellsUpon TCR cross-linking in vitro, splenocytes from both LTalpha-/- and LTbeta-/- mice failed to produce IL-4 and IL-10 due to a reduction in NK T cells.
Oosterwegel et al. (1999)IL-4T cellsSimilarly, when CTLA-4-/- CD4+ T cells from mice lacking CTLA-4, B7. 1, and B7.2 were stimulated in vitro with anti-CD3 Ab and wild-type APC, these CTLA-4-/- CD4+ T cells produced IL-4 even during the primary stimulation, whereas CD4+ cells from B7.1/B7.2-/- mice did not produce IL-4.
Lavelle et al. (2004)IL-4T cellsHere, we demonstrate that immunization with antigen in the presence of CT induced a population of antigen-specific CD4(+) T cells that produced IL-10 in the absence of IL-4, in addition to cells that coexpressed IL-4 and IL-10 or produced IL-4 only.
Takeuchi et al. (1998)IL-4T cellsP518-529-specific T cells produced IL-2 and IFN-gamma, but not IL-4 or IL-10, whereas P1182-1194-specific T cells produced IL-4 and IL-10, but not IL-2 or IFN-gamma Adoptive transfer of these peptide-specific T cells into naive BALB/c nude mice resulted in development of uveoretinitis only in the P518-529 case.
Hamel et al. (2008)IL-4T cellsFurthermore, there was a significant reduction in the PG-specific CD4(+) T cell recall response as well as significantly fewer PG-specific CD4(+) T cells producing IFN-gamma and IL-17, but not IL-4.
Rivero et al. (1998)IL-4T cellImmune mice develop a T cell-mediated response to MAG assessed by in vitro proliferation and accompanied by the release of IFN-gamma, whereas IL-4 is not detectable in the same culture super-natants.
Jin et al. (2009)IL-4T cellsAllergic airway hyperresponsiveness-enhancing gammadelta T cells develop in normal untreated mice and fail to produce IL-4/13, unlike Th2 and NKT cells.
Gysemans et al. (2000)IL-4T-cellT-cell cytokines (IL-2, IL-4, IL-10, and gamma-interferon) were absent in all mice until 48 h after transplantation.
Anthony et al. (2001)IL-4T cellsAdditionally, the anti-TCR Vbeta10p-specific cellular immune response was mediated by CD4+ T cells and these T cells did not produce IL-4 or IL-5.
Wesley et al. (2008)IL-4T cellsWe found that Valpha14i NK T cells become activated and produce significant levels of IFN-gamma, but do not proliferate or produce IL-4 following MCMV infection.
Nakagawa (1997)IL-4T cellsFurthermore it was demonstrated that NK1.1+ T cells in aly/aly mice were unable to produce efficiently IL-4 upon in vivo stimulation with anti-CD3.
Segal et al. (2002)IL-4T cellsSurprisingly, glioma-specific CD4+ T cells produce IL-10 but neither IL-4 nor IFN-gamma, and glioma rejection is compromised in IL-10(-/-) hosts.
Hattori et al. (2006)IL-4T cellsSera from TG nude mice with keratitis reacted with alpha-internexin on Western blot analysis, and the T cells of these mice on stimulation with alpha-internexin exhibited proliferation responses and produced IL-2, IFN-gamma, and TNF-alpha, but not IL-4 or IL-5.
Carrier et al. (2007)IL-4T cellsTo address this issue, we generated a TGF-beta1-transgenic (Tg) mouse in which TGF-beta is linked to the IL-2 promoter and T cells transiently overexpress TGF-beta upon TCR stimulation but produce little or no IL-2, IL-4, IL-10, IL-13, or IFN-gamma.
Naiki et al. (1999)IL-4T cellsThe peritoneal exudate T cells in wild type (wt) mice on day 3 after infection produced IL-4 upon TCR alpha beta stimulation, whereas those in TCR beta-/-, beta 2m-/-, or J alpha 281-/- mice showed no IL-4 production upon the stimulation, indicating that NK1.1+ alpha beta T cells are the main source of IL-4 production at the early phase of Salmonella infection.
Mayack and Berg (2006)IL-4T cellsSpecifically, activated Jak3(-/-) CD4(+) T cells produce IL-10, TGF-beta, and IFN-gamma, but not IL-2 or IL-4, and are unable to proliferate in vitro.
Kitazawa and Streilein (2000)IL-4T cellsWhen pulsed with OVA, these cells were capable of inducing proliferation among DO11.10 T cells in vitro, but the responding cells produced neither IFN-gamma nor IL-10 and IL-4.
Naiki et al. (1992)IL-4T cellAll of the autoreactive cloned T cell lines produce significant IL-4 but no detectable IL-2 or IFN-gamma.
Boom et al. (1990)interleukin-4T cellsMycobacterium-specific T cells from both BALB/c and B10.D2 mice produced interleukin-2 and no interleukin-4.
Brimnes et al. (2003)IL-4T cellsIn the absence of influenza, these OVA-specific T cells produced little IL-2, IL-4, IL-10, and IFN-gamma, but with infection, both CD4+ and CD8+ T cells made high levels of IL-2 and IFN-gamma.
Ofosu-Appiah et al. (1996)IL-4T cellWhen cultured with either heparan sulfate or Concanavalin A, the T cell clones produced high levels of IL-4 and IL-5 with no detectable IL-2 or IFN-gamma.
Naiki et al. (2000)IL-4T cellsThe gammadelta T cells produced IFN-gamma but neither IL-4 nor IL-13 in response to heat-killed Salmonella, whereas both IFN-gamma and IL-13 but no IL-4 was produced by the gammadelta T cells stimulated with immobilized anti-TCRgammadelta mAb.
Kuzushita et al. (2006)interleukin 4T cellsThe CD4(+) T cells obtained from mice immunized with nonstructural 5-transduced dendritic cells produced interferon gamma, but not interleukin 4, when stimulated with nonstructural 5.
Kuzushita et al. (2006)interleukin 4T cellsIn contrast, T cells derived from mice immunized with hepatitis C virus core-transduced dendritic cells produced neither interferon gamma nor interleukin 4 when stimulated with core protein.
Hoyle et al. (1998)IL-4T-cellIntracellular cytokine production was assayed by fluorescence-activated cell sorter (FACS), and the expanded T-cell cultures produced IL-2, IFN-gamma, and tumor necrosis factor-alpha (TNF-alpha), but not IL-4.
Weir et al. (2006)IL-4T cellsMOG(35-55)-specific T cells from PPNK-deficient and wild-type mice produced IFNgamma and TNFalpha but no IL-4 or IL-10, indicative of a Th1 phenotype.
Kelso et al. (1991)IL-4T cellsA requirement for an undefined, filler cell-dependent signal for development of IL-4-secreting clones was suggested by the finding that clones of normal CD4+ and CD8+ T cells activated in an anti-CD3-induced, filler cell-free system exclusively produced IFN-gamma and IL-3 without detectable IL-4 or IL-6.
Mohapatra et al. (2010)IL-4T cellsCD4+ T cells from B6 VC+ mice also expressed significantly more IL-4 on day 0, but this difference was absent on days 2 and 7.
Caprio-Young et al. (2006)IL-4T cellsIntriguingly, the anergic splenic T cells, although nonproliferative and unable to produce IFN-gamma or IL-4, secrete significant amounts of IL-2.
Bell et al. (2003)IL-4T cellsIntriguingly, the anergic splenic T cells, although nonproliferative and unable to produce IFN-gamma or IL-4, secrete significant amounts of IL-2.
Furukawa et al. (2002)interleukin-4T cellsAlso, normal T cells cultured with 1/2D-M(phi) did not produce interleukin-4 when transwell cultures were performed in the presence of anti-MCP-1 monoclonal antibody.
Jump and Levine (2002)IL-4T cellsUpon stimulation, only CD45RB(low)CD4(+) PP T cells produce IL-10, whereas secretion of IL-2, IL-4, and IFN-gamma was not detected.
Quinn et al. (2001)IL-4T cellT cell clones and hybridomas from both of these T cell groups were responsive to APC pulsed with GAD65(524--543) or whole rGAD65. p524-reactive cells appeared to be regulatory upon adoptive transfer into young NOD mice and could inhibit insulin-dependent diabetes mellitus development, although they were unable to produce IL-4, IL-10, or TGF beta upon antigenic challenge.
Bradley et al. (1991)IL-4T cellsWhen restimulated with specific antigen in vitro, CD4+ T cells from mice primed 5 to 7 days previously by subcutaneous administration of keyhole limpet hemocyanin (KLH) in adjuvant, produced high levels of interleukin 2 (IL-2), IL-4, and IL-3, and little or no interferon gamma (IFN-gamma) or IL-5.
Chan et al. (2001)IL-4T cellsUnlike the findings for NK cells, NK T cells were dramatically diminished in Vav-1(-/-) mice and splenocytes from Vav-1 mutant mice failed to produce IL-4 in response to in vivo CD3 stimulation.
Zhan et al. (1995)IL-4T cellsIn this study, we further analyzed the response of T cells from Brucella-infected mice and SBP-immunized mice and demonstrated that CD4(+)-enriched cells from SBP-immunized mice also produced significant amounts of IL-4, which was not detected in bulk cultures of spleen cells from infected mice.
Suto et al. (2008)IL-4T cellsIL-21–producing CD4+ T cells under Th17-polarizing conditions were also negative for intracellular IL-4 and IFN-?
Nicolson et al. (2002)IL-4T cellsMOG35-55-specific T cells lacking Jnk2 showed a T(h)1 cytokine profile with IFN-gamma, but no IL-4 or IL-5 production.
Rodrigues et al. (1999)IL-4T cellsWe found that bulk cells from DNA-immunized mice had CD4 and CD8 T cells that produced gamma interferon (IFN-gamma) but not interleukin-4 (IL-4) or IL-10.
Takano et al. (2007)IL-4T cellOrally administered capsicum extract and capsaicin did not change the T cell subset (CD4(+) and CD8(+)), Th1 (IFN-gamma(+)) and T2 (IL-4(+)) ratio.
Juillard et al. (1995)interleukin-4T lymphocytesA proliferative response occurred only late after immunization and the primed T lymphocytes produced interleukin-2, but no interleukin-4.
Wesley et al. (2008)IL-4T cells14i NK T cells do not produce detectable amounts of IL-4 during MCMV infection in either tissue (data not shown).
Wu et al. (2007)IL-4T cellsThe Vgammadelta T cell receptor (TCR) repertoire in kidney showed expansion of a subset of cells that expressed Vgamma6/Vdelta1 genes and that used canonical TCR Vgamma6/Vdelta1 sequences in the CDR3 region of the TCR. gammadelta T cells that were sorted from the kidneys expressed TGF-beta but not IL-4, IL-10, or IFN-gamma. gammadelta T cells also expressed the activating receptor NKG2D and the NKG2D adaptor molecule DAP12.
Katamura et al. (1995)IL-4T cellsProstaglandin E2 at priming of naive CD4+ T cells inhibits acquisition of ability to produce IFN-gamma and IL-2, but not IL-4 and IL-5.
Wesley et al. (2008)IL-4T cells14i NK T cells do not proliferate nor produce IL-4 following MCMV-induced activation.
Yang et al. (1991)IL-4T cellsT cells induced by p146-171 and p467-171 or a mixture of these two peptides were mainly CD4+ and produced interleukin (IL-2) and interferon-gamma (IFN-gamma) but not IL-4 upon antigen stimulation in vitro.
Tolomeo et al. (2009)IL-4T cellsThese T cells produced IL-2 and IFN-gamma, but not IL-4 or IL-10, indicating a Th1 bias of the beta2GPI-specific response.