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Chen et al. (2010)deltaT-cellIn order to elucidate the feature of T-cell immune status in umbilical cord blood (CB) from humans, the expression levels of CD3gamma, delta, epsilon, and zeta chain genes in CD4(+) and CD8(+) T cells of CB were analysed by real-time PCR.
Chen et al. (2010)deltaT cellsIn CD4(+) T cells, the expression levels of CD3gamma, delta, and zeta genes (16.54+/-6.49, 3.53+/-1.15, and 5.48+/-1.10%) from CB were significantly higher than those from PB (P=0.001, P=0.017, and P=0.000, respectively).
Chen et al. (2010)deltaT cellsIn conclusion, the present study characterized the expression pattern of CD3gamma, delta, epsilon, and zeta chain genes in CD4(+) and CD8(+) T cells from CB, which might be very useful for further understanding the feature of T-cell immune status in umbilical cord blood.
Gumbs et al. (2009)deltaT-cellBACKGROUND: This disease process, also known as gamma/delta (gamma/delta) TCL because of the expression of the T-cell receptor gamma/delta chain, tends to present in young male patients.
Cornfield and Gheith (2009)deltaT lymphocytesMETHODS: Using multicolor flow cytometry and sequential gating techniques, NK cells and T lymphocytes expressing TCR alpha/beta and TCR gamma/delta were identified and quantitated in 30 benign and 30 malignant effusions.
Bjurstöm et al. (2008)deltaT cellThe cells expressed the alpha1, alpha4, beta2, beta3, gamma1 and delta GABAA channel subunits and formed functional, extrasynaptic-like GABA channels that were activated by 1 microM GABA. 100 nM and higher GABA concentrations decreased T cell proliferation.
Gollob et al. (2008)deltaT cellsThe goal of this review is to briefly outline our findings with hopes of putting our most recent studies concerning the dichotomy between alpha/beta TCR and gamma/delta TCR expressing, CD4-CD8- (double negative-DN) T cells in the context of a balanced immune response against Leishmania and to discuss the implications of these findings toward our understanding of human leishmaniasis.
Chandesris et al. (2007)deltaT-cellCerebrospinal fluid analysis revealed large granular lymphomatous cells with CD3+ CD4- CD8- CD7+ CD16- CD56- surface antigens, expressing the gamma delta T-cell receptor.
Devilder et al. (2006)deltaT cellPotentiation of antigen-stimulated V gamma 9V delta 2 T cell cytokine production by immature dendritic cells (DC) and reciprocal effect on DC maturation.
Maley et al. (2006)deltaT-cellImmune cells expressing CD3, CD4, CD8, gamma delta (gammadelta) T-cell receptors (TCR), CD79alpha cytoplasmic (cy) (B cells) and NKp46 [natural killer (NK) cells] antigens were identified immunohistochemically and cells expressing mRNA for interferon-gamma (IFN-gamma) were labelled by in-situ hybridization.
Baseggio et al. (2006)deltaT-cellThe expression of TCR-gamma delta/CD3 complex in neoplastic gamma delta T-cell.
Holtmeier and Kabelitz (2005)deltaT cellWhile most T cells use a CD3-associated alpha/beta T cell receptor as antigen recognition structure, a second population of T cells expresses the alternative gamma/delta T cell receptor. gamma/delta T cells are a minor population in the peripheral blood but constitute a major population among intestinal intraepithelial lymphocytes.
Lapolla et al. (2005)deltaT cellIn 62 GDM patients and 74 women with normal glucose tolerance (NGT), and their babies, we assessed total lymphocytes, T lymphocyte subsets CD3 and CD8 expressing T cell receptor (TCR) alpha/beta or gamma/delta, CD16 and CD19, pancreatic autoantibodies and cytokines (IL-5, IL-2, soluble receptor IL-2).
De Rosa et al. (2004)deltaT cellsIn addition, we show that the representation of naive T cells is higher among alphabeta than gammadelta T cells in adults and that the low frequency of naive gammadelta T cells in adults reflects ontological differences between the two major gammadelta subsets, which are distinguished by expression of Vdelta1 vs Vdelta2 delta-chains.
Couedel et al. (2004)deltaT cellThrough the analysis of the rearrangement status of the TCR gamma, delta, and beta loci in human gamma delta T cell clones, expressing either the TCR V delta 1 or V delta 2 variable regions, we show that the rate of partial rearrangements at the delta locus is consistent with an allelic exclusion process.
Spadaro et al. (2004)deltaT cellsRESULTS: PsA and RA patients had, with respect to controls, lower values (both as percentages and in absolute numbers) of PB T cells expressing gamma/delta TCR.
Go and Wester (2004)deltaT-cellThe presence of HPS at diagnosis and expression of the gamma/delta T-cell receptor (TCR) by tumor cells were associated with poor survival, whereas age was not.
Bodman-Smith et al. (2002)deltaT cellA significant increase in the absolute numbers of T cells coexpressing the gamma delta (gammadelta) T cell receptor and CD8 were also seen in the HIV-LTNP patients compared with controls (P = 0.002).
Fierro et al. (2001)deltaT-cellMost patients showed aberrant phenotypes, with a loss of T-cell lineage markers and expression of cytotoxic molecules or gamma-delta chain of the T-cell receptor.
Gardner and Kearse (1999)deltaT cellMost T lymphocytes express on their surfaces a multisubunit receptor complex, the T cell antigen receptor (TCR) containing alpha, beta, gamma, delta, epsilon, and zeta molecules, that has been widely studied as a model system for protein quality control.
Ohsawa et al. (1999)deltaT-cellTumor cells were frequently stained positively with monoclonal antibodies (mAbs) for T lymphocytes, but were negative for T-cell receptor (TCR) beta and delta chain expression.
Roth et al. (1998)deltaT lymphocytesTwo subpopulations of human T lymphocytes expressing different antigen receptors, alpha/beta and gamma/delta, emigrate into inflamed tissues in distinctive patterns.
Cara et al. (1998)deltaT cellsAfter stable transfection of CEM T cells and following challenge with HIV-1, viral production was completely inhibited in cells transduced with the vector producing both delta-gag and EDN and delayed in cells producing delta-gag alone.
Sleckman et al. (1998)deltaT cellsTCR alpha and delta variable region genes are assembled at different stages of T-cell development, and fully assembled TCR alpha and delta variable region genes must be expressed in distinct lineages of T cells, alpha beta and gamma delta, respectively.
Krangel et al. (1998)deltaT-cellThe T-cell receptor (TCR) alpha/delta locus includes a large number of V, D, J and C gene segments that are used to produce functional TCR delta and TCR alpha chains expressed by distinct subsets of T lymphocytes.
Mincheva-Nilsson et al. (1997)deltaT cellsDecidua contains large numbers of lymphocytes, of which CD56+ cells dominate, followed by T cells expressing either alpha beta or gamma delta TCR.
Schaberg et al. (1997)deltaT cellPBLs were obtained from healthy volunteers and analyzed by flow cytometry using antibodies for the detection of CD3, CD4, CD8, CD19, CD56, CD57, CD45RO, CD45RA, alpha/beta and gamma/delta T cell receptor epitopes.
Olivry et al. (1997)deltaT-cellThe hyperplasia of epidermal T-cells expressing the gamma/delta T-cell receptor appears specific to canine atopic dermatitis compared with its human counterpart.
Halary et al. (1997)deltaT cellControl of self-reactive cytotoxic T lymphocytes expressing gamma delta T cell receptors by natural killer inhibitory receptors.
Halary et al. (1997)deltaT cellsWithin V gamma 9V delta 2 T cells, KIR were mainly expressed by clones exhibiting a strong lytic activity against Daudi cells.
Hedin et al. (1997)DeltaT cellsDelta-opioid receptors expressed by Jurkat T cells enhance IL-2 secretion by increasing AP-1 complexes and activity of the NF-AT/AP-1-binding promoter element.
Li et al. (1997)deltaT cellsRESULTS: When this novel culture system was used, gamma delta T cells mainly expressing V gamma 9/V delta 2 encoded TCR were selected and expanded rapidly from 4.8 +/- 3.4% to 46.2 +/- 8.3%.
Spinozzi et al. (1996)deltaT-cellMEASUREMENTS: CD3+, CD4+, and CD8+ T cells from patients and controls were examined by two-color flow cytometry for coexpression of V delta 1 and V delta 2 isoforms of the gamma delta T-cell receptor.
Catálfamo et al. (1996)deltaT cellsThe TCR expressed by these gamma delta T cells--V gamma I/V delta 5--is unusual in peripheral blood lymphocytes, and its specificity is clearly different from that observed in a high percentage of gamma delta T cells from PBL, which express the common TCR V gamma 9/V delta 2.
Harabuchi et al. (1996)deltaT-cellSeven EBV-positive cases expressed a TCR-delta chain with rearranged TCR-gamma or -delta genes whereas both EBV-negative cases corresponded to alpha beta T-cell lymphoma, which expressed a TCR-beta chain with a rearranged TCR-beta gene.
Caldwell et al. (1996)deltaT cellsExpansion of activated T cells expressing the T-cell receptor (TCR) gamma/delta, CD45RO, and HLA-DR antigens is a prominent feature of acute infection with Ehrlichia chaffeensis in humans.
Esin et al. (1996)deltaT cellIn the Turkish group, 9.3% (median value) of the CD3+ peripheral blood T cells expressed the gamma delta T cell receptor.
Thorp et al. (1996)deltaT-cell(a) Motile and non-motile T-cell lines were compared for expression of the alpha, beta I, beta II, gamma, delta, epsilon, eta, zeta and theta isotypes by Western blotting.
Fricke et al. (1996)deltaT cellAlthough the majority of peritoneal T cells expressed alpha beta T cell receptor (TcR), 7% expressed gamma delta TcR, a proportion which was significantly higher than that in peripheral blood (PBMC) (approximately 4%).
Kersten et al. (1996)deltaT cellsSpecimens from all healthy adults tested showed intense proliferation in response to both bacterial preparations; at 6 days, the responding cells were mainly T cell blasts, of which high percentages (up to 80%) were gamma delta T cells, most expressing V delta 2/V gamma 9.
Troncone et al. (1996)deltaT cellGood markers of potential CD include the presence of serum endomysial antibodies, a high count of intraepithelial lymphocytes and subtle pathological alteration such as increased density of intraepithelial lymphocytes expressing gamma delta T cell receptor, signs of activated mucosal cell-mediated immunity, coeliac-like intestinal antibody pattern, and positive rectal gluten challenge.
Rothenberg et al. (1996)deltaT cellsThese T cells expressed predominantly the gamma delta T cell receptor and mediated non-MHC-restricted cytotoxicity against EBV-infected target cells.
Moreau et al. (1996)deltaT cellsThe percentages of CD4+, CD8+, or double-negative CD4-CD8- (DN) T cells (most of the latter expressing the gamma delta TCR) for 8 symptomatic groups were compared with those for a reference group of asymptomatic HIV-1-infected patients.
Isaza et al. (1996)deltaT cellMost T cells in the granuloma expressed alpha beta T cell receptor (TCR) (40.3%) whereas only a few (6.7%) expressed gamma delta TCR.
Yamanaka et al. (1996)deltaT cellFlow cytometric analysis using TCR-gamma/delta-1 showed that 2.2%, of T cells in the tonsil obtained from patients with recurrent or chronic tonsillitis expressed the gamma delta-T cell receptor.
Behr (1996)deltaT cellThe recent discovery of the set of ligands leading to simulation of Human gamma delta T cell subsets expressing the V gamma 9V delta 2 chains of the T cell receptor may give new insights about their mode of activation and their potential role in the induction of malaria pathology.
Crossley et al. (1996)deltaT cellTwo patients had an unusually high percentage of T cells expressing the gamma delta T cell receptor, which decreased after therapy.
Holcombe et al. (1995)deltaT cellOBJECTIVES: To examine the expression of the natural killer (NK) antigen CD56, and T cell receptor delta chain antigen (TCR delta), expressed on the gamma delta T cell subset, in patients with scleroderma, and to correlate levels of expression with clinical characteristics.
Cope et al. (1995)deltaT-cellUsing panels of T-cell lines and clones expressing high levels of p75 TNF-R, we found that p75 sTNF-R production upon stimulation is a feature common to all subsets of T cells, including cells of the CD4-CD8- double negative phenotype expressing either alpha beta or gamma delta T-cell receptors (TCR).
Vila et al. (1995)deltaT cellsHuman gamma delta T cells expressing the V gamma 9/V delta 2 T-cell receptor have been previously found to proliferate in response to certain microorganisms and to expand throughout life, presumably because of extrathymic activation by foreign antigens.
Lang et al. (1995)deltaT cellEarly activation of human V gamma 9V delta 2 T cell broad cytotoxicity and TNF production by nonpeptidic mycobacterial ligands.
O'Hanlon et al. (1995)deltaT cellGamma delta T cell receptor gene expression by muscle-infiltrating lymphocytes in the idiopathic inflammatory myopathies.
Livak et al. (1995)deltaT cellOur observations are in conflict with the idea that alpha beta and gamma delta T cells derive from distinct precursors and suggest that commitment of a common precursor to the gamma delta lineage depends upon expression of a gamma delta T cell receptor.
Livak et al. (1995)deltaT cellWe propose that the intrathymic T cell lineage decision is determined by a competition between the production of functional gamma delta and beta-pre-T cell receptor complexes.
Peyrat et al. (1995)deltaT cellCharacterization of dual T cell receptor (TCR) delta-chain expressors and alpha beta T cells expressing V delta 3J alpha C alpha-encoded TCR chains.
Hartman et al. (1995)deltaT cellsAlthough the majority of peritoneal cavity lymphocyte (PCL) T cells expressed alpha beta T cell receptor (TcR), up to 17% expressed gamma delta TcR.
Norazmi et al. (1995)deltaT cellThe lymphocyte subset expressing the gamma delta T cell receptor is increased in several infectious diseases including HIV infection.
Chao et al. (1995)deltaT cell(Am J Reprod Immunol 1993;29:1-4) found that all early intradecidual CD3+ T cells expressed either T cell receptor (TCR) alpha/beta or gamma/delta but that the expression of the CD3+/TCR complex was down-regulated.
Chao et al. (1995)deltaT cellsRESULTS: Intradecidual CD3+ T cells expressed either TCR alpha/beta or gamma/delta, and the level of expression of the CD3/TCR complex was down-regulated in normal pregnancy, anembryonic pregnancy, and recurrent spontaneous abortion.
Mastovich et al. (1994)deltaT-cellFlow cytometry studies showed cells with an unusual T-cell phenotype expressing the gamma delta T-cell receptor and restricted expression of the V delta 1 but not the V delta 2 protein, indicating the clonal nature of the proliferation.
Hettmann and Cohen (1994)deltaT cellSince the alpha beta and gamma delta T cell receptor heterodimers are expressed in a mutually exclusive fashion and since TCR genes are sequentially activated during T cell ontogeny, transcriptional activation and repression must at least in part determine T lineage-specific and developmental-specific expression of these genes.
Loh et al. (1994)deltaT cellsThe transfectant T cells each expressing different gamma delta receptors all produced IL-2 after stimulation with plastic bound anti-CD3 Ab, but only those expressing V gamma 9 responded to stimulation with SEA in the presence of an autologous lymphoblastoid B cell line.
Wilson et al. (1994)deltaT cellDuring T cell development in the thymus, T cell receptor (TCR) alpha, beta, gamma, and delta genes are rearranged and expressed.
Preudhomme et al. (1994)deltaT-cell"Illegitimate" rearrangements of the immunoglobulin heavy chain (IgH) gene and incomplete rearrangements involving V delta 2 and D delta 3 segments of the T-cell receptor delta (TcR delta) gene are seen in some cases of AML, and AML post-SMD, and can be detected by a sensitive PCR method.
Arranz et al. (1994)deltaT cellIntestinal antibody pattern of coeliac disease: association with gamma/delta T cell receptor expression by intraepithelial lymphocytes, and other indices of potential coeliac disease.
Kaur et al. (1994)deltaT cellHuman peripheral gamma delta T cells, coexpressing the V gamma 9/V delta 2 chains of the T cell receptor, lyse Daudi cells but not Raji cells.
Bouchonnet et al. (1994)deltaT cellsBoth resting CD4+ and CD8+ T cells expressing TCR-alpha beta and -gamma delta responded to previously activated cells, including cells with both the naive and memory phenotypes.
Tanaka et al. (1994)deltaT cellsAll gamma delta T cells stimulated by MEP expressed V gamma 2/V delta 2 receptors.
Pham et al. (1994)deltaT cellsIn contrast, the percentage of T cells expressing the gamma/delta receptor and that of CD2+/CD57+ cells were similar in both groups of patients.
Perry et al. (1994)deltaT cellDuring the same time period, cells expressing both alpha beta and gamma delta T cell receptors demonstrated a 2-6 fold increase in frequency.
Martínez et al. (1993)deltaT cellMost DN T lymphocytes express the gamma/delta T cell receptor (TcR), but a minor fraction of them, in both humans and mice, express the alpha/beta TcR.
Bergstresser et al. (1993)deltaT cellsDendritic epidermal T cells (DETC) in mice form part of a primitive system of epithelial-resident T cells characterized by the expression of gamma delta T-cell receptors (TCR).
Fujita et al. (1993)deltaT-cellA case of cutaneous T-cell lymphoma expressing gamma delta T-cell receptors.
Fujita et al. (1993)deltaT-cellCutaneous T-cell lymphoma expressing gamma delta T-cell receptors (TCRs) is rare; only a few cases have been reported.
Mentzel et al. (1993)deltaT-cellInterestingly, lymphocytes expressing the gamma delta T-cell receptor (TCR tau delta) were significantly decreased both before (PB 1.2 +/- 0.1%; BM 0.8 +/- 0.1%) and after 6 weeks of therapy (PB 0.7 +/- 0.1%; BM 0.7 +/- 0.1%) as compared with healthy controls (PB 2.4 +/- 0.2%; BM 2.3 +/- 0.2%).
Mentzel et al. (1993)deltaT-cellHowever, the proportion of the gamma delta-T-cell subpopulation expressing the delta TCS1 phenotype was markedly increased before (PB 42 +/- 3.5%; BM 31 +/- 3%) and especially after 42 days of therapy (PB 77 +/- 12%; BM 45 +/- 2%) as compared with that in normal subjects (PB 19 +/- 2%; BM 9.7 +/- 0.8%).
Kirk et al. (1993)deltaT cellsCharacterization of T cells expressing the gamma/delta antigen receptor in human renal allografts.
Vollenweider et al. (1993)deltaT cellOnly LAK cells expressing the gamma/delta T cell receptor (TCR) were found in a significantly increased percentage in fractions containing conjugates indicating that gamma/delta-TCR+ LAK cells were preferably bound to target cells at the time of separation.
Sugerman et al. (1993)deltaT cellThe majority of clones were CD3+CD4-CD8+alpha beta+gamma delta-, although three clones were CD3+CD4+CD8-alpha beta+gamma delta- and one clone was CD3+CD4-CD8- and expressed the gamma delta T cell receptor.
Alaibac et al. (1993)deltaT-cellIt has been suggested that T lymphocytes expressing gamma delta T-cell receptors could play an important role in defence against some intracellular infectious pathogens.
Alaibac et al. (1993)deltaT lymphocytesThe present study was undertaken to characterize the occurrence and variable delta gene expression of T lymphocytes expressing the gamma delta T-cell receptor in oriental cutaneous leishmaniasis.
Flanagan et al. (1993)deltaT cellAlso, a panel of gamma delta T cell clones expressing V gamma 8/V delta 3 isolated from a different donor, were all highly cytotoxic against BSM, indicating that these target cells can be recognised by effector cells expressing a TCR other than the V gamma 9/V delta 2 receptor.
Poggi et al. (1993)deltaT-cellLiver-cell suspensions cultured in the presence of phytohemagglutinin and recombinant interleukin 2 gave rise to large proportions of CD3+ lymphocytes expressing either alpha/beta or gamma/delta T-cell receptors.
Bagot (1993)deltaT-cellThe prognostic value of expression of one of the two types of T-cell receptors for the antigen, alpha/beta or gamma/delta, remains to be established.
Skeen and Ziegler (1993)deltaT cellsThe proportion of the CD3+ population expressing the gamma/delta T cell receptor remained elevated for 6-7 wk, while the absolute numbers of peritoneal gamma/delta T cells declined gradually over this time period, reflecting a decrease in both the number of lymphocytes and the percentage of these that were CD3+.
Robijn et al. (1993)deltaT cellsThe results demonstrate that intestinal TCR gamma/delta T cells from healthy individuals constitute a phenotypically heterogeneous population expressing V delta regions that differ from their systemic counterparts.
Sturgess et al. (1993)deltaT-cellExpression of the gamma/delta T-cell receptor was significantly increased in patients with dermatitis hepetiformis (P < 0.0005) and in both untreated (P < 0.0005) and treated coeliac patients (P < 0.05) compared with controls.
Gotlieb et al. (1993)deltaT cellsPrecursor thymocytes grown in the presence of IL-1 and Con A develop to CD3+ T cells that express either the TcR alpha/beta or gamma/delta complex on the cell surface (1).
He and Kabelitz (1993)deltaT-cellDifferential effects of interleukin-7 and interleukin-2 on T-cell receptor gamma delta-expressing cells within CD4-CD8- postnatal human thymocytes.
Munk et al. (1993)deltaT cellsFrozen sections of all 5 onchocerca nodules tested demonstrated an increased number of CD3+ cells in the vicinity of the adult worm, in all cases expressing the alpha/beta T cell receptor and in 2 patients also expressing the gamma/delta T cell receptor; 60% of T cells expressed the activation marker Ki67.
Jezewska et al. (1993)deltaT lymphocytesExpression of T cell antigen receptor tau/delta (TCR-1) on T lymphocytes, demonstrated by BB3 MoAb (vs V delta 2), was significantly higher in malignant effusions as compared with non-malignant ones.
Soukiasian et al. (1992)deltaT cellsThe majority of human peripheral blood and lymphoid tissue T cells express the TCR alpha/beta heterodimer, while the TCR gamma/delta is expressed on only a small subset of T lymphocytes.
Miossec et al. (1992)deltaT lymphocytesT lymphocytes express either the alpha/beta or the gamma/delta receptor (TCR) in a mutually exclusive fashion.
Kikuchi et al. (1992)deltaT cellMurine T cell lines and hybridomas derived from the epidermis that express the V gamma 1.1C gamma 4V delta 6C delta TCR and may, therefore, recognize an autoantigen, secrete cytokines spontaneously in culture.
Kikuchi et al. (1992)deltaT cellsThese results demonstrate that transfection of the gamma delta TCR confers to recipient T cells the phenotype of constitutive activation, as well as dependence on engagement of the VNR as an accessory molecule.
Nanno et al. (1992)deltaT cellGamma/delta T cell antigen receptors expressed on tumor-infiltrating lymphocytes from patients with solid tumors.
Nanno et al. (1992)deltaT cellThe expression of gamma/delta T cell antigen receptors (TcR) in T cell lines or clones derived from tumor-infiltrating lymphocytes (TIL) from patients with solid tumors was investigated. gamma/delta TcR T cell lines were derived from TIL from patients with Wilms tumor, sarcoma or metastatic melanoma by stimulation with autologous tumor cells alone and recombinant interleukin 2 and they exhibited nonspecific cytotoxicity against autologous and allogeneic tumor cells, or cells of the K562 or the MEL21 tumor cell lines.