Viewing affirmative mentions of transcription of IL2 (H. sapiens)

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Weaver et al. (2007)IL-2 geneTranscription of the IL-2 gene is tightly controlled by the combined actions of multiple transcriptional activators.
Weaver et al. (2007)IL-2However, the contribution of sequences in the IL-2 core promoter and the architecture of the IL-2 regulatory region to setting levels of IL-2 transcription are not understood.
Henics et al. (1994)IL-2Six major cytoplasmic proteins bound in vitro transcribed RNA probes containing sequences from the 3'-UTR of normal human IL-2 (3'-IL-2), GM-CSF (delta 2R1), and the virally altered MLA IL-2 (3'-IL-2 PV) mRNA.
Sawami et al. (1992)IL-2Addition of IL-2 also increased mRNA levels for c-fos, c-myc, IL-2 receptor alpha, and IL-2 receptor beta chain.
Nordmann et al. (1989)interleukin 2Cyclosporin A was used as a valuable tool to gain more insight into the quantitative aspects of interleukin 2 production, on the basis of the assumption that transcription of the interleukin 2 gene is completely inhibited shortly after administration of cyclosporin A.
Modiano et al. (1991)IL2 geneHowever, when PKC inhibitors were added after IL2 gene transcription had already proceeded for 3-4 h, the IL2 in the culture supernatants was still reduced by 30-80%, and intracellular IL2 was increased by up to 50%.
Hopkins and Failla (1999)IL-2 geneSynthesis of this cytokine is regulated by alterations in transcription of the IL-2 gene and the stability of IL-2 mRNA.
Krönke et al. (1984)TCGFTo define further both the specificity of CsA and the level at which it interferes with lymphokine gene expression, we have studied its effects on TCGF mRNA accumulation as well as TCGF gene transcription.
Krönke et al. (1984)TCGFThese data suggest a relatively selective action of CsA on TCGF gene transcription.
Waldmann et al. (1984)IL-2In HTLV-I infected cells the 42 kdalton long open reading frame (LOR) protein encoded in part, by the pX region of HTLV-I may act as a transacting transcriptional activator that induces transcription of the IL-2 receptor gene thus providing an explanation for the constant association of HTLV-I infection of lymphoid cells and IL-2 receptor expression.
Ferguson et al. (2001)IL-2 promoterHere, we studied the kinetic mechanism of basal transcription at the IL-2 promoter using a human in vitro RNA polymerase II transcription system.
Wilkinson and Euhus (2001)IL-2The immunosuppressive drug CsA is known to block native IL-2 transcription but has no affect on the adenoviral-mediated IL-2 or lac-Z transgene.
Waldmann (1987)IL-2In HTLV-I-infected cells, the 42-Kd long open reading frame (tat) protein encoded in part by the tat region of HTLV-I may act as a transacting activator that induces transcription of the IL-2 receptor gene, thus providing an explanation for the constant association of HTLV-I infection of lymphoid cells and IL-2 receptor expression.
Hallet et al. (1989)IL-2 geneA combination of phorbol ester plus calcium ionophore was very efficient in all clones tested (17/17) in inducing transcription of the IL-2 gene.
Boumpas et al. (1991)IL-2 geneNuclear transcription run-off assays showed that high doses of dexamethasone inhibited the transcription of the IL-2 gene but not that of the IL-2R gene.
Boumpas et al. (1991)IL-2 geneDexamethasone both inhibits transcription of the IL-2 gene and decreases the stability of IL-2 mRNA.
Bunting et al. (2006)interleukin-2Control of interleukin-2 gene transcription: a paradigm for inducible, tissue-specific gene expression.
Bunting et al. (2006)IL-2 geneThe mechanisms that control IL-2 gene transcription have been studied in detail for the past 20 years and our current understanding of the nature of the inducible and tissue-specific controls will be discussed.
Shaw et al. (1987)IL2CsA interrupted ongoing transcription of IL2 by a mechanism not dependent on the induction of a new protein.
Cullen (1986)IL-2In the absence of the trans-activating viral gene product, tat-III, IL-2 transcripts specific for the HIV LTR were present in low abundance in transfected cells and showed a low translational efficiency, when compared with IL-2 mRNAs transcribed from other viral promoters.
Cullen (1986)IL-2Coexpression of tat-III resulted in a marked increase in the steady state level of IL-2 mRNAs transcribed from the HIV LTR, and these mRNAs also demonstrated a specific enhancement of their translational efficiency.
Kudla et al. (2006)IL2We have shown that the efficiency of mRNA production from GFP, IL2, and Hsp70-family genes in mammalian cells correlates with the silent-site GC content of these genes.
Umadome et al. (1988)IL-2Although the expression of IL-2 or IL-4 mRNA was not detected in fresh leukemic cells, the expression of IL-2 mRNA, but not IL-4 mRNA, was induced by phytohemagglutinin stimulation, and the leukemic cells proliferated.
Lewis et al. (1993)IL-2Effects of rolipram and CI-930 on IL-2 mRNA transcription in human Jurkat cells.
Lewis et al. (1993)IL-2IL-2 transcription was greatly inhibited by 1 microM CsA, whereas neither 10 microM rolipram nor 10 microM CI-930 had any effect on steady-state levels of IL-2 mRNA.
Paliogianni et al. (1993)interleukin 2 geneProstaglandin E2 and other cyclic AMP elevating agents inhibit interleukin 2 gene transcription by counteracting calcineurin-dependent pathways.
Paliogianni et al. (1993)IL-2 geneWe have therefore tested the hypothesis that this phosphatase may be a target for the inhibitory effects of cAMP on IL-2 gene transcription.
Fujino et al. (1997)IL-2Furthermore, pretreatment with Oxo-M enhanced PHA-induced mRNA expression of the alpha and beta subunits of IL-2 receptors and DNA synthesis.
Arya and Gallo (1984)TCGF geneNuclear transcription experiments suggested that the TCGF gene was more actively transcribed in cells treated with TPA and phytohemagglutinin than in cells treated with phytohemagglutinin alone.
Arya and Gallo (1984)TCGF geneThe transcription of the TCGF gene was further increased when interleukin 1 was included along with TPA and phytohemagglutinin.
Depper et al. (1985)IL-2Interleukin 2 (IL-2) augments transcription of the IL-2 receptor gene.
Depper et al. (1985)IL-2IL-2 stimulation results in increased accumulation of IL-2 receptor mRNA within 4 hr, while an increase in IL-2 receptor gene transcription is detected within 30 min in isolated nuclei.
Waldmann (1986)IL-2In HTLV-I-infected cells, the 42-kDa long open reading frame (tat) protein encoded in part by the tat region of HTLV-I may act as a transacting activator that induces transcription of the IL-2-receptor gene, thus providing an explanation for the constant association of HTLV-I infection of lymphoid cells and IL-2-receptor expression.
Garrity et al. (1994)Interleukin-2Interleukin-2 transcription is regulated in vivo at the level of coordinated binding of both constitutive and regulated factors.
Belldegrun et al. (1990)IL-2The parental noncloned population expressed mRNA for TNF, lymphotoxin, GM-CSF and TGF-beta but not for IL-1 beta or IL-2.
Bahbouhi and al-Harthi (2004)IL-2Analysis of interleukin 2 (IL-2) mRNA expression using real-time reverse transcription PCR (RT-PCR) of the HIV-enriched population demonstrated a higher level of IL-2 mRNA, by approximately four cycles, and an 8-fold increase in IL-2 expression, as evaluated by intracellular staining and flow cytometric analysis, in comparison to gp41-negative cells.
Raab et al. (2001)interleukin 2In this study, we show that CD28 can cooperate with VAV/SLP-76 adaptors to upregulate interleukin 2/4 transcription independently of TCR ligation.
Junger et al. (1997)IL-2 mRNAIL-2 mRNA transcription in HTS-treated Jurkat cells was measured by reverse transcriptase polymerase chain reaction.
Hémar and Dautry-Varsat (1990)IL2 geneWe have previously shown that CsA prevents the constitutive transcription of the IL2 gene in these cells.
Faist et al. (1993)IL-2The Northern blot analysis of IL-2 mRNA expression in the lysates of PHA-stimulated cell cultures after trauma could not detect any mRNA signal for IL-2, in contrast to the control cells.
Paetkau (1985)IL2Moreover, the immunosuppressive agent cyclosporin A specifically inhibits the induction of mRNA for IL2 and certain other lymphokines, without affecting the expression of the majority of other proteins.
Tsuboi et al. (1994)IL-2 promoterEarlier studies on the IL-2 gene expression showed that overexpression of calcineurin (CN), a Ca2+/calmodulin-dependent protein phosphatase, can stimulate transcription from the IL-2 promoter through the NF-AT-binding site.
Ketzinel and Kaempfer (1999)IL-2Moreover, during IL-2 gene induction, splicing of IL-2 precursor transcripts becomes inhibited, resulting in a transient mRNA wave.
Brinkmeier et al. (1992)IL-2The reaction of the IL-2 solution with an anti IL-2 antibody neutralized the inhibitory effect on the Na+ currents, indicating a specific effect of the peptide growth factor interleukin-2 on muscular Na+ channels.
Vacca et al. (1994)IL-2 geneThe role of HIV tat, which is the main enhancing factor for viral LTR, in the regulation of IL-2 gene transcription has been studied following transient expression of the tat gene in phorbol ester and calcium ionophore-activated Jurkat cells transfected with IL-2 promoter-chloramphenicol acetyltransferase reporter constructs.
Roifman et al. (1985)IL-2By comparing IL-2 activity of recombinant IL-2 to that of cell-derived IL-2, we have demonstrated that each of the preparations are equally active in several murine and human IL-2 proliferation assays and that IL-2 is the active moiety in these assays.
Horst and Flad (1985)IL-2The changes in the concentration of the high affinity binding sites are considered as being the result of: receptor stimulation by PHA and/or IL-2 (days 1-2); receptor down regulation by extensive IL-2 production (day 3); increase of unoccupied IL-2 receptor after disappearance of IL-2 from the medium (days 4-6) and cessation of receptor synthesis and receptor breakdown (days 7-10).
Hemler et al. (1984)IL-2After reexposure to antigen, a 10- to 30-fold increase in the level of the IL-2 receptor was rapidly induced, with the peak level of IL-2 receptor expression occurring at 15-30 hr.
Arya and Gallo (1985)TCGF geneThe results reported here show that the transcription of the TCGF gene was not impaired in cultured HTLV-III-infected cells.
Plitnick et al. (2001)IL-2Further studies using ELISA demonstrated that LTA blocks IL-2 detection and function by binding directly to IL-2.
Hopkins and Failla (1999)IL-2 mRNAPrevious studies showed that low Cu status also decreased the level of IL-2 mRNA in activated Jurkat cells by 50%.
Vacca et al. (1990)IL2Dexamethasone treatment of the Jurkat T77 cell clone inhibited the enhancing effect of 12-O-tetradecanoylporbol-13-acetate (TPA) and the calcium ionophore A23187 on the interleukin 2 (IL2) mRNA levels and gene transcription from intact nuclei.
Chu et al. (2003)interleukin-2[A controlled clinical study on the efficacy of recombinant human interleukin-2 in the treatment of pulmonary tuberculosis].
Corrias et al. (1998)IL-2The combination of IL-2 gene transfection and the xenograft model in nude mice was chosen on the basis of the low or absent expression of HLA class I antigen in human NB tumors.
Ha et al. (1993)IL-2The level of IL-2 in the predialysis group was markedly lower compared to the control, HD and CAPD groups (as 3.1 +/- 0.8 unit vs. 8.8 +/- 2.2 unit, 11.8 +/- 3.0 unit and 14.9 +/- 3.4 unit, respectively, p < 0.05); the difference between the control and dialytic groups was not statistically significant.
Smith (2006)IL2IL2 itself is a small globular glycoprotein comprised of four antiparallel ?
Reichert et al. (1998)IL-2We have previously demonstrated that interleukin-2 (IL-2) receptors, IL-2 protein, and mRNA for IL-2 are present in human carcinomas in vitro and in vivo.
Ferguson et al. (2001)IL-2 promoterMoreover, we found that the synthesis of a four nucleotide RNA was necessary and sufficient to rapidly complete the escape commitment step of transcription at the IL-2 promoter.
Ferguson et al. (2001)IL-2 promoterWe present a kinetic model for a single round of basal transcription at the IL-2 promoter that provides insight into mechanisms by which the IL-2 gene is transcriptionally regulated.
Ferguson et al. (2001)IL-2 promoterWe found that the rate of functional preinitiation complex formation limits the overall rate of transcription at the IL-2 promoter under the conditions described here.
el-Farrash et al. (1995)IL-2The cell line (EMF) was initially IL-2 dependent and then became IL-2 independent after gradual withdrawal of the IL-2.
Du and Wolfe (2003)C125A rhIL-2A kinetic model was developed to describe recombinant human protein disulphide isomerase (rhPDI)-assisted folding of a substrate protein, C125A recombinant human interleukin-2 (C125A rhIL-2).
Kahan et al. (1991)IL-2Lineweaver-Burk plots of the Michaelis-Menton equation suggested rapamycin inhibits IL-2 signal transduction in competitive, and IL-6 signal transduction in noncompetitive fashion, suggesting distinctive components of the various cytokine-receptor mechanisms.
Fletcher and Goldstein (1987)IL-2A more precise understanding of the mechanism of action IL-2, in particular its role in the induction of the IL-2 receptor, and aspects of the control of IL-2 production was also achieved.
Dohlsten et al. (1988)IL2This discrepancy in kinetics was not due to an increased absorption of IL2 by the CD4+45R+ cells during the first day of culture.
Salmeron et al. (1992)IL-2Furthermore, 2 IFN gamma-producing CTL clones tested expressed mRNA for both IFN gamma and IL-2.
Astoul et al. (1994)IL-2[The kinetics of IL-2 after pleural administration in the treatment of neoplastic pleurisy].
Xu (1992)interleukin 2[Determination of interleukin 2 activity in patients with pulmonary tuberculosis].
Yang et al. (1991)IL-2Serum levels of IL-2 were detected by enzyme-linked immunosorbent assay 2 weeks after cessation of therapy.
Sosman et al. (1988)IL-2At an IL-2 dose of 3 X 10(6) U/m2 per day, bolus IL-2 was less immunostimulatory than continuous-infusion IL-2.
Akiyama et al. (1997)IL-2 geneAP-1 is the primary target for steroid-mediated repression of IL-2 gene transcription, but NF-AT appears to be the main target of cyclosporin.
Lopez et al. (1998)IL-2Expression of IL-2 activity was not detected; however, mRNA encoding IL-2 was produced and translatable in vitro.
Whittington and Faulds (1993)IL-2Adverse effects accompanying high dose, intravenous IL-2 therapy can be severe, with cardiovascular, pulmonary, haematological, hepatic, neurological, endocrine, renal and/or dermatological complications frequently requiring doses to be withheld.
Attema et al. (2002)interleukin-2 geneTo determine the role of chromatin structure in the regulation of interleukin-2 gene transcription, nucleosome assembly across the IL-2 promoter region was examined using in vitro chromatin reconstitution assays.
Kudla et al. (2006)IL2We demonstrated that GC content does not significantly affect the cellular mRNA lifetimes of GFP, IL2, and Hsp70 genes.
Martinez et al. (1993)IL-2Analysis of cytokine gene expression revealed that IL-2 transcription peaked within the initial 24 hr of culture, whereas IL-5 transcription was maximal at 120 hr of culture.
Rainbow et al. (2008)IL-2The present review focuses on allele-specific expression assays that can be used to quantify genotype-determined expression differences such as those observed for IL-2, where the susceptibility allele is transcribed 2-fold less than the resistance allele.
Eugen-Olsen et al. (1997)IL-2An increase in IL-2 production may be a part of this mechanism since addition of serotonin to in vitro cultures of PHA-stimulated cells increases the expression of mRNA for IL-2 and IFN-gamma.
Kindler et al. (1995)IL-2 mRNAThe appearance of IL-13 mRNA, but not IL-4 mRNA, was detected under the same stimulation conditions as for IL-2 mRNA.
Kumagai et al. (1988)IL-2In cells treated with PDB/ionomycin, maximum expression was observed at 3 hr for IL-2 mRNA and at 6 hr for TFR mRNA, whereas the level of IL-2 receptor mRNA reached a peak 24 to 48 hr after stimulation.
Starkebaum et al. (1991)IL-2In addition, no mRNA message for IL-2 was detectable in these cells.
Grabstein et al. (1986)IL 2The expression of mRNA for IL 2, IL 2R, and IFN-gamma correlated very well with the levels of protein observed.
Bosco et al. (2000)IL-2However, relatively little is known about the biochemical mechanisms involved in IL-2 signal transduction in these cells.
Huland et al. (1994)IL-2We describe here a mainly topical interleukin-2 (IL-2) application in pulmonary metastatic renal-cell carcinoma: a high-dose long-term inhalation of IL-2 (90% of IL-2 dose) and low-dose systemic subcutaneous IL-2 (10% of IL-2 dose) and systemic subcutaneous interferon alpha (IFN alpha).
Benbernou et al. (1995)IL-2 geneMoreover, these data provide further evidence that the induction of IL-2 gene transcription is highly sensitive to an elevation of cAMP, whereas IL-4 gene transcription appeared to be less affected.