Viewing affirmative mentions of gene expression of IL2 (H. sapiens) in fibroblasts

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Abdullah et al. (1991)IL2fibroblastsInterleukin 2 (IL2), an immune modulator produced by T lymphocytes, was tested in vitro for its effects on human diploid fibroblasts.
Gulino et al. (1990)IL-2 genefibroblastsTo study the cell-specific expression of the IL-2 gene, we transfected the intact human IL-2 gene, including 2.0 kb of 5' and 0.3 kb of 3' flanking sequences, into mouse NIH-3T3 fibroblasts and BFS lymphoma T cells and into human epithelial HeLa cells.
Tartour et al. (2000)IL-2fibroblastsTo reverse this defect and based upon animal studies, we initiated a phase I clinical trial of gene therapy in which various doses of xenogeneic monkey fibroblasts (Vero cells) genetically engineered to produce human IL-2 were administered intratumorally in 8 patients with metastatic solid tumours.
Fakhrai et al. (1997)IL-2fibroblastsSimilar levels of IL-2 were expressed by human fibroblasts transduced with pLXSN vectors employing the preproinsulin (pLXSN-iIL2) or tissue factor (pLXSN-tIL2) leader sequences (range in IL-2 units/10(6) cells/24 h pLXSN-iIL2 = 375-625 vs. pLXSN-tIL2 = 90-440).
Duncombe et al. (1991)IL2fibroblastsLocal production of IL2 activated cytotoxic cells which would be generated during CMV infection would damage uninfected as well as infected marrow fibroblasts and thereby could compromise haemopoietic growth factor production by marrow fibroblasts.
Sobol et al. (1999)IL-2fibroblastsThe purpose of this study was to determine the safety, toxicity, and antitumor immune response following S.C. immunizations with a mixture of irradiated, autologous tumor cells and autologous fibroblasts that were genetically modified to express the gene for interleukin 2 (IL-2) in patients with colorectal carcinoma.
Umetsu et al. (1986)IL 2fibroblastsIt is postulated that fibroblasts differ from classical antigen-presenting cells in that fibroblasts are incapable of stimulating the production of IL 2 in resting T cells.
Plaisance et al. (1992)IL-2fibroblastsData from [125I]rIL2 cross-linking experiments show the simultaneous expression of two IL-2 binding peptides of 70 and 55 kDa respectively on embryonic young fibroblasts as on lymphoid activated cells.
Ackermann et al. (2002)IL-2fibroblastThis study examined whether tumor and fibroblast cell lines established from Ewing tumor patients could be efficiently transfected with the IL-2 gene.
Ackermann et al. (2002)IL-2fibroblastsIL-2 production was generally lower in fibroblasts as compared to Ewing tumor cell lines.
Bubenik et al. (1988)IL-2 genefibroblastWe have prepared a retroviral expression construct, pPS-IL-2, in which human IL-2 cDNA has been inserted into the polylinker region, and have used the retroviral vector to introduce the functional IL-2 gene into a fibroblast cell line, RAT-1.
Veelken et al. (1997)interleukin-2-gene-transfectedfibroblastsA phase-I clinical study of autologous tumor cells plus interleukin-2-gene-transfected allogeneic fibroblasts as a vaccine in patients with cancer.
Veelken et al. (1997)IL-2fibroblastA clone (KMST 6.14) of an immortalized human fibroblast line that stably secreted 5290 IU IL-2 per 10(6) cells and per 24 hr was obtained by cationic lipofection with an expression construct for human IL-2 and Neo(r).
Gruss et al. (1996)IL-2fibroblastsHuman fibroblasts express functional IL-2 receptors formed by the IL-2R alpha- and beta-chain subunits: association of IL-2 binding with secretion of the monocyte chemoattractant protein-1.
Ozawa et al. (2003)IL-2fibroblastsExpression of IL-2 receptor beta and gamma chains by human gingival fibroblasts and up-regulation of adhesion to neutrophils in response to IL-2.
Plaisance et al. (1992)IL-2fibroblastsHere we show that the mAbs IOT14 and MIK beta 1 directed against the IL-2 binding sites of the IL-2R alpha and IL-2R beta respectively, stain human embryonic fibroblasts early in their life span.
Plaisance et al. (1992)IL-2fibroblastsIn this study, we have investigated the expression of the alpha and beta chains of the IL-2 receptor (IL-2R alpha, IL-2R beta) both at the membrane and at transcriptional levels during the lifespan of human embryonic fibroblasts.
Plaisance et al. (1993)IL-2fibroblastsIn this study, we have investigated the IL-2R gamma gene expression in several human embryonic fibroblasts which express other components of the IL-2 receptor (IL-2R).
Kuida et al. (1992)IL-2fibroblastsAlthough IL-2 receptor beta chain (IL-2R beta) expressed in various lymphoid cell lines binds IL-2 with an intermediate affinity, IL-2R beta expressed in fibroblasts is unable to bind IL-2, suggesting that IL-2R beta is on its own not sufficient for generating the intermediate-affinity receptor and that lymphoid-specific regulatory control may be operated to allow IL-2R beta to bind IL-2.
Le and Vilcek (1987)IL-2fibroblastsAllogeneic fibroblasts failed to exert accessory activity when exogenous interleukin 2 (IL-2) was used as the stimulus for IFN-gamma production.
Engel et al. (1998)IL-2fibroblastsWe transfected fibroblasts of the patient with an IL-2 gene expression vector using a cationic liposome reagent.
Ozawa et al. (2004)IL-2fibroblastsEndogenous IL-15 sustains recruitment of IL-2Rbeta and common gamma and IL-2-mediated chemokine production in normal and inflamed human gingival fibroblasts.
Badolato et al. (1997)IL-2fibroblastUnlike IL-2, which is secreted only by T cells, IL-15 is expressed preferentially by nonlymphoid tissues, epithelial, and fibroblast cell lines and by activated monocytes/macrophages.
Demeure et al. (1995)IL-2fibroblastsThis conclusion is based on three observations: (1) highly purified human naive CD4 T cells, of neonatal or adult origin, develop into Th2 effectors upon repetitive cycles of stimulation with anti-CD3 monoclonal antibody (mAb) cross-linked to CD32-B7 transfected L fibroblasts followed by IL-2 expansion; (2) IL-4 protein is readily detectable in the concentrated supernatant fluids of priming cultures performed in the presence of anti-IL-4 receptor mAb; and (3) addition of anti-IL-4 or anti-IL-4 receptor mAb at priming markedly inhibits the acquisition of IL-4- and IL-5-producing capacity while enhancing that of interferon-gamma.
Kelly et al. (1982)lymphokinefibroblastThe inability to suppress lymphokine production and arrest persistent immune reactivity, coupled with the known ability of lymphokines to augment fibroblast collagen production, offers a a reasonable explanation for the accumulation of tissue collagen in scleroderma.
Tan et al. (1990)lymphokinefibroblastsOur results suggest that in rheumatoid arthritis, synovial fibroblasts actively participate in joint inflammation by lymphokine production.
Gruaz et al. (2010)IL-2fibroblastFifteen factors out of 27 were not significantly induced by CEsHUT, including cytokines specifically produced by T cells (i.e., IL-2, IL-4, IL-5, IL-9, IL-13, and IL-17), cytokines and chemokines produced by a variety of cells (i.e., IL-7, IL-10, IL-12, IL-15, CCL11 and CXCL10) and growth factors (i.e., basic fibroblast growth factor – FGF, platelet-derived growth factor - PDGF, vascular endothelial growth factor - VEGF).
Lemaire and Dubois (1983)lymphokinefibroblastIn vitro suppression of fibroblast growth inhibitory lymphokine production by asbestos.
Selvey et al. (2004)IL-2fibroblastsThus NIH3T3 and primary mouse embryonic fibroblasts (MEFs) were: a) treated with the cytokines IL-1beta, IL-2, IL-6, IL-8 and TGF-beta for 3, 6, 12, 24, and 48 hours; b) grown on collagens I, IV and V; c) treated with fibronectin, con-A and matrigel; and d) co-cultured with a range of HBC (human breast cancer) cell lines of varied invasive and metastatic potential.
Yang et al. (1995)IL-2fibroblastsWe previously reported that human naive CD4 T cells differentiate into effector cells producing type 1 (IL-2, IFN-gamma) and type 2 (IL-4, IL-5, IL-10) cytokines after priming with anti-CD3 mAb presented on irradiated CD32-transfected mouse L fibroblasts, in the absence of exogenous cytokine.
Corrigall et al. (2001)IL-2fibroblastFunctional IL-2 receptor beta (CD122) and gamma (CD132) chains are expressed by fibroblast-like synoviocytes: activation by IL-2 stimulates monocyte chemoattractant protein-1 production.
Rousseau et al. (2006)IL-2fibroblastsWe studied the feasibility, safety, and immunologic efficacy of an IL-2- and CD40L-expressing recipient-derived tumor vaccine consisting of leukemic blasts admixed with skin fibroblasts transduced with adenoviral vectors encoding human IL-2 (hIL-2) and hCD40L.
Wakasugi et al. (1987)interleukin 2fibroblastsThe purified material shares several biological properties with monocyte IL-1, since it could induce the proliferation of murine thymocytes, the production of interleukin 2 by phytohemagglutinin-stimulated cloned HSB2 T cells, and the proliferation of human fibroblasts.
Bernauer et al. (1993)IL-2fibroblastInterleukin-2 (IL-2), interferon-gamma, transforming growth factor-beta (TGF-beta), platelet-derived growth factor (PDGF), basic fibroblast growth factor (bFGF), tumour necrosis factor-alpha and proliferating cells (as identified with the antibody Ki-67) were found in both pemphigoid patients and normal controls.
Mackensen et al. (1997)interleukin-2fibroblastsThus it is likely that vaccination with autologous tumor cells plus interleukin-2 gene transfected allogeneic fibroblasts had induced not only local accumulation but also an increase in the frequency of circulating tumor specific CTL.
Brouty-Boyé et al. (1998)IL-IIfibroblastsMoreover, MMS fibroblasts showed a higher expression of ICAM-1 and VLA-4 than NS fibroblasts; and (4) cytokines: IL-II, RANTES and MIP-1alpha were produced in higher amounts by BM than by NS fibroblasts.
Greenwel et al. (1993)lymphokinefibroblastWe report that the novel lymphokine fibroblast-stimulating factor 1, produced within hepatic schistosomal egg granulomas, stimulates liver fat-storing cells (FSC) in vitro to proliferate and express fibronectin genes.
Bruns et al. (1994)IL-2fibroblastsMETHODS: We studied the following immune parameters to get more insight into SSc: autoantibodies (antinuclear antibodies (ANA), anti-Scl-70, anticentromere antibodies (ACA) subsets of lymphocyte subpopulations and markers of their activation, as well as serum levels of IL-2, the soluble IL-2 receptor (SIL-2R), IL-6 and its correlation to N-terminal procollagen-III propeptide (P III P), and finally, the IL-6 production by SSc and normal dermal fibroblasts.
Chopra et al. (1997)IL-2fibroblastThe serum levels of tumor necrosis factor alpha (TNF alpha), granulocyte/macrophage-colony-stimulating factor, basic fibroblast growth factor (BFGF), IL-7 and IL-2 were significantly elevated in patients with stages II and III carcinoma and the serum level of tumor necrosis factor beta (TNF beta) was slightly elevated in patients with stage II carcinoma only.
Takebe et al. (1988)lymphokinefibroblastsThe SR alpha expression system was 1 or 2 orders of magnitude more active than the SV40 early promoter in a wide variety of cell types, including fibroblasts and lymphoid cells, and was capable of promoting a high level of expression of various lymphokine cDNAs.
Kunz and Ibrahim (2009)IL-2fibroblastsIL-7 is a well-known cytokine of the IL-2/IL-15 family and has been shown to be expressed by stromal cells (e.g., in bone marrow and thymus), epithelial cells, endothelial cells, fibroblasts, smooth muscle cells, and keratinocytes.
Fireman et al. (1999)IL-2AFbThe expression of AFb mRNA for IL1-alpha and beta, TGF-beta, IFN-gamma, IL-2, IL-4, IL-5 and IL-6 was determined by RT-PCR.