Viewing affirmative mentions of gene expression of IL2 (H. sapiens) in mononuclear cells

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Andersson and Sander (1989)IL-2mononuclear cellsBlood mononuclear cells (MNC) were cultured and stimulated by mitogenic anti-CD3 antibody to produce IL-2.
Elfenbein et al. (1984)interleukin-2mononuclear cellsWe found that interleukin-2 production, upon optimal stimulation, was impaired for mononuclear cells of most recipients early after marrow transplant.
Silveira-Lacerda et al. (2010)interleukin-2PBMCResults also showed that cis-[RuCl(2)(NH(3))(4)]Cl presents a dual role on PBMC stimulating proliferation and interleukin-2 (IL-2) production at low concentration and inducing cytotoxicity, inability to proliferate, and inhibiting IL-2 production at high concentration.
Petrov and Lijnen (2000)IL-2PBMCTo evaluate the role of interleukin-2 (IL-2) in the inhibition of the proliferation of human peripheral blood mononuclear cells (PBMC) by calcium channel blockade, the effect of nifedipine, which blocks the L-type calcium channel on the proliferation, the IL-2 expression and the IL-2 production in human PBMC, was compared with the effect of mibefradil, which blocks both L- and T-type calcium channels with a more selective blockade of T-type channels.
Kitamura et al. (1989)IL-2mononuclear cellsThe ELISA specifically detected human IL-2 in cell culture supernatants and serum, and also in extracts from mitogen-stimulated peripheral blood mononuclear cells.
Smith and Roberts-Thomson (1989)IL-2mononuclear cellsIn contrast, in the seronegative arthritis patient group only SF mononuclear cells were hyporesponsive to mitogenic stimulation and there were no significant differences in IL-2 production or IL-2 receptor upregulation as compared with control subjects.
Smith and Roberts-Thomson (1989)IL-2mononuclear cellsNo significant correlations were observed between IL-2 inhibitor levels and mitogenic responses, IL-2 production and IL-2 receptor upregulation on peripheral blood and SF mononuclear cells but an inverse correlation was noted between SF mitogenic responses and the expression of selected activation markers.
Lomnitzer et al. (1984)IL-2mononuclear cellsCon A-treated mononuclear cells (S cells) cultured with PHA-activated allogeneic or autologous responder cells showed reduced [3H]thymidine incorporation and IL-2 production as compared to activated R cells alone.
Bruserud and Moen (1984)interleukin 2mononuclear cellsCulture conditions which would enhance production of interleukin 2 (IL-2) from PHA-stimulated peripheral blood mononuclear cells (PBM) were studied.
Krajewski et al. (1983)IL-2mononuclear cellsWhen IL-2 production by PHA stimulated cells was measured culture supernatants from the three T helper cell leukaemias all showed normal or high levels of activity, when compared to normal blood mononuclear cells, whereas the T suppressor cell leukaemia showed no activity.
Faist et al. (1993)IL-2 proteinmononuclear cellThe major regulatory level of interleukin-2 (IL-2) release under stressful conditions was determined via parallel analysis of IL-2 messenger RNA (mRNA) expression and IL-2 protein synthesis in mitogen-stimulated peripheral blood mononuclear cell (PBMCs) cultures on consecutive days postinjury.
McElhaney et al. (1990)IL2PBMCAfter stimulation with a 1/320 dilution of the same influenza vaccine, cultures of PBMC from young controls showed a significantly greater increase in IL2 production than the cultures of PBMC from the elderly group of patients.
Su et al. (1991)Interleukin 2mononuclear cellsInterleukin 2 (IL-2) production and lymphocyte proliferation of peripheral blood mononuclear cells (PBMC) were measured before and after the treatment.
Alcocer-Varela et al. (1989)interleukin-2mononuclear cellsWe studied the in vitro production of interleukin-2 (IL-2), the expression of IL-2 receptors, the absorption of IL-2, and spontaneously expanded suppressor cell function by mononuclear cells from 15 patients with systemic lupus erythematosus (SLE) and 15 healthy control subjects.
Vie and Miller (1986)IL 2mononuclear cellsOn average, 16.3% of peripheral blood mononuclear cells can produce IL 2 in such clonal cultures (mean of 12 determinations; SD = 5.6%).
Toossi et al. (1986)IL-2mononuclear cellsPPD-induced expression of T cell IL-2 receptors was 5.9 times less in peripheral blood mononuclear cells of patients with tuberculosis as compared with healthy tuberculin reactors.
Venkataraman and Westerman (1990)IL-2MNCsThe effects of radiation on the kinetics of Interleukin-2 (IL-2) production and utilization by mononuclear cells (MNCs) were studied.
Venkataraman and Westerman (1990)IL-2MNCsMoreover, the supernatants from irradiated MNCs collected as late as 72 h after the initiation of culture contained more than 30% of the total IL-2 produced compared to less than 8% in supernatants from non-irradiated cells.
Welte et al. (1984)IL2mononuclear cellsUsing OKT3 monoclonal antibody as a mitogen, we have studied interleukin 2 (IL2) production and proliferation in peripheral blood mononuclear cells (PBMC) of 23 patients receiving bone marrow transplants.
Welte et al. (1984)IL2PBMCEndogenous IL2 production was not detectable (less than 0.2 U/mL) in PBMC of 18 patients and was very low in PBMC from five patients (0.5 to 1.5 U/mL), as compared to normal controls (median 3.5 U/mL) or pretransplant patients (median 1.5 U/mL).
Welte et al. (1984)IL2PBMCThe low IL2 production was associated with defective OKT3-induced proliferation of PBMC in 19 of 23 patients studied.
Welte et al. (1984)IL2PBMCThis study demonstrates that PBMC in patients after BMT have a defect of IL2 production but are able to express IL2 receptors in response to OKT3 antibody and to proliferate normally upon addition of hpIL2.
Lindqvist et al. (1987)IL-2PBLThese findings make it possible to analyse the capacity of antibody 60.3 to interfere with IL-2 production and receptor expression by phorbol ester or phytohaemagglutinin (PHA)-treated human PBL.
Lindqvist et al. (1987)IL-2PBLFACS analysis showed that IL-2 receptor expression was unaffected by antibody 60.3, whereas DNA synthesis of the same P(Bu)2-treated PBL was inhibited.
Lindqvist et al. (1987)IL-2PBLThese results indicate that a cell-to-cell adhesion step is necessary for the production of IL-2, but not for the expression of its receptor on both PHA- and P(Bu)2-treated human PBL.
Siebelink et al. (1990)IL-2PBMCAlso IL-2 production levels of mitogen-activated PBMC from naturally infected symptomatic cats were significantly reduced.
Carver et al. (1991)Interleukin-2mononuclear cellsInterleukin-2 production by stimulated mononuclear cells was higher in the NT+ compared with the NT- group at 2 months of age (0.90 +/- 0.28 U/mL, 0.27 +/- 0.11 U/mL, respectively); neither formula-fed group differed significantly from the breast-fed group.
Clerici et al. (1991)interleukin-2PBMCPeripheral blood mononuclear cells (PBMC) were tested at 1, 5, 13, 16, and 19 months for T helper (Th) cell function by in vitro-generated proliferation and interleukin-2 production in response to four synthetic peptides corresponding to env of HIV.
Hirooka et al. (1990)interleukin-2mononuclear cellsThe present study demonstrates the usefulness of quantitating human interleukin-2 produced by human blood mononuclear cells and that there exists an impaired production of interleukin-2 in Graves' disease.
Monner et al. (1986)IL2mononuclear cellsAbsolute capacity for IL2 production by human peripheral blood mononuclear cells (PBMC) was studied using 12-O-tetradecanoylphorbol 13-acetate (TPA) and calcium ionophore A23187, which act synergistically, to induce lymphokine synthesis.
Manolagas et al. (1986)IL-2PBMWe used peripheral blood mononuclear cells (PBM) activated in vitro with phytohemagglutinin to study 1) the relationship between 1,25(OH)2D3 receptor expression, IL-2 production, and 1,25(OH)2D3-induced inhibition of PBM proliferation in connection with the cell cycle; 2) the effect of 1,25(OH)2D3 on PBM activation and on the expression of activation-related molecules including the IL-2 receptor, and 3) the role of calcium in the antiproliferative effect of the hormone. 1,25(OH)2D3 receptor expression occurred when PBM entered the G1a phase of the cell cycle.
Manolagas et al. (1986)IL-2PBMThese findings support the contention that 1,25(OH)2D3-induced inhibition of PBM proliferation is mediated through selective inhibition of IL-2 production.
Roilides et al. (1991)interleukin-2mononuclear cellsHelper T-cell function was evaluated in 34 children infected with human immunodeficiency virus type 1, by assessing interleukin-2 production after stimulation of peripheral blood mononuclear cells with recall antigens (influenza virus, tetanus toxoid), allogeneic HLA, and phytohemagglutinin.
Kuo et al. (1993)IL-2HMNCInterleukin-2 (IL-2) production and cell-mediated cytotoxicity (CMC) were also determined in HSV-1-infected HMNC but were lower than those found in uninfected HMNC.
Tsang et al. (1986)interleukin-2PBMCThe effects of Isoprinosine (ISO) on interleukin-2 (IL-2) production by human peripheral blood mononuclear cells (PBMC) were investigated.
Tsang et al. (1986)IL-2PBMCTreatment (of human PBMC) with ISO enhanced IL-2 production by PBMC from 7 of 10 normal individuals.
Tsang et al. (1986)IL-2PBMCThese results suggest that the increase in IL-2 production following treatment of PBMC with ISO may be mediated through the activation of a distinct subset of IL-2 producing cells.
Briggs et al. (1999)IL-2mononuclear cellsTo evaluate molecular mechanisms that might account for the heterogeneity in the in vitro responsiveness of individual subjects' peripheral blood mononuclear cells (PBMC) to immunosuppressive drugs, the authors quantitated in normal human cells the suppressive effects of the glucocorticoids prednisolone and methylprednisolone and of cyclosporine on interleukin-2 (IL-2) mRNA expression and IL-2 production, as well as the stimulatory effect of these drugs on IkappaBalpha mRNA expression.
Briggs et al. (1999)IL-2PBMCAs expected, cyclosporine was significantly more suppressive than either glucocorticoid of IL-2 mRNA expression and IL-2 production by mitogen-stimulated PBMC, with variable degrees of inhibition in cells from individual subjects.
Rueda et al. (1989)interleukin 2MNCPlatelet (PLT) contamination in preparations of mononuclear cells (MNC) produces a significant decrease (P less than 0.02) in lymphoproliferative activity and interleukin 2 (IL2) production.
Lin et al. (2002)IL-2PBMCThe inhibitory mechanisms may involve the blocking of interleukin-2 (IL-2) and interferon-gamma (IFN-gamma) production, since compound 3 attenuated IL-2 and IFN-gamma production of PBMC in a dose-dependent manner.
Suthanthiran (1987)IL-2mononuclear cellsCyclosporine inhibited anti-CD3-mediated expression of IL-2 receptors and IL-2 factor production by peripheral blood mononuclear cells (PBM).
Donnelly et al. (1986)interleukin-2PBMCIn order to account at least partially for this immunopotentiation by NPT-15392, we examined the effects of this compound on interleukin-2 (IL-2) production by cultures of mitogen-activated human peripheral blood mononuclear cells (PBMC).
Donnelly et al. (1986)IL-2PBMCPretreatment of PBMC with NPT-15392 for 2-4 h prior to activation with Con A was sufficient to achieve maximum enhancement of IL-2 production (20-40% average increase).
Donnelly et al. (1986)IL-2PBMCExposure of PBMC to NPT-15392 for longer periods (i.e. 24 h) did not result in higher levels of IL-2 production.
Rappaport and Dodge (1982)IL-2mononuclear cellRemoval of adherent cells from mononuclear cell populations also resulted in enhanced IL-2 production.
Prasad et al. (2006)interleukin-2mononuclear cellsCorrection of interleukin-2 gene expression by in vitro zinc addition to mononuclear cells from zinc-deficient human subjects: a specific test for zinc deficiency in humans.
Prasad et al. (2006)IL-2MNCsIt was further shown that either in vivo zinc supplementation or the addition of zinc in vitro to MNCs from zinc-deficient subjects results in correction of the gene expression of IL-2 and IL-2Ralpha.
Prasad et al. (2006)IL-2MNCsTherefore, it was proposed that in vitro addition of zinc to MNCs for correction of gene expression of IL-2 in humans may be used as a specific test for zinc deficiency.
Venkataraman (1992)IL-2MNCsTo better understand the effects of cryopreservation on various immunocompetent cell functions, we have examined the interleukin-2 (IL-2)-producing activities of frozen mononuclear cells (MNCs) from healthy subjects.
Venkataraman (1992)IL-2MNCsBoth the unfractionated and monocyte-depleted, frozen MNCs produced significantly larger quantities of IL-2 than fresh cells.
Venkataraman (1992)IL-2MNCsSimilar to freezing, L-leucine methyl ester (Leu-OMe) treatment (to eliminate IL-1 and prostaglandin E-2 (PGE-2)-secreting cells) also increased the IL-2-producing activities of fresh cells, but freezing no longer enhanced the production of IL-2 by Leu-OMe-treated cells, suggesting that (1) both the freezing process and Leu-OMe treatment have similar effects on IL-2 production, (2) the increased IL-2 secretion by frozen MNCs is independent of IL-1, and (3) inactivation of PGE-2-secreting cells during the freezing procedure is responsible for increased IL-2 secretion.
Ellner et al. (1990)interleukin-2mononuclear cellsRegulation of antigen-induced blastogenesis and interleukin-2 expression by adherent mononuclear cells in humans infected with Schistosoma mansoni.
Ellner et al. (1990)interleukin-2mononuclear cellsBlastogenesis and production of interleukin-2 (IL-2) by peripheral blood mononuclear cells showed a significant correlation when streptolysin O but not when soluble worm antigenic preparation (SWAP) was used as stimulus.
Cooley et al. (1989)IL2PBMCStatistical analysis of the clinical factors possibly affecting lymphokine synthesis showed that in vivo cyclosporin A did not affect the in vitro capacity of PBMC to produce cytokines, although steroid therapy had a negative effect on IL2 production.
Dohlsten et al. (1986)IL-2mononuclear cellsHistamine, a modulator of various immune functions, inhibits the production of interleukin 2 (IL-2) and interferon-gamma (IFN-gamma) by polyclonally activated human blood mononuclear cells.
Chen et al. (1995)interleukin-2mononuclear cells[Human interleukin-2 gene transfer and expression inhibits hepatitis B virus surface antigen expression and enhances LAK activity of peripheral blood mononuclear cells of normal adults].
Chen et al. (1995)interleukin-2mononuclear cellsThese results indicated that retroviral vector-packaging cell line can efficiently transfer and express interleukin-2 cDNA in human somatic cells and low level expression of interleukin-2 potentially inhibits hepatitis B virus surface antigens expression and enhances LAK activity of peripheral blood mononuclear cells of normal persons.
Stadler et al. (1982)IL 2mononuclear cellsHuman interleukin 2 (IL 2) was produced under serum-free conditions by stimulating mononuclear cells with concanavalin A (Con A) in the presence of phorbol myristate acetate (PMA) and hydroxyurea.
Merryman et al. (1993)interleukin-2mononuclear cellsMETHODS: The effects of NO and SNO-GSH on DNA synthesis, interleukin-2 (IL-2) production, IL-2 receptor expression, and cGMP accumulation were determined in phytohemagglutinin-activated peripheral blood mononuclear cells (PBMC) and spleen T cells.
Carlsson et al. (1987)interleukin 2mononuclear cellsHuman mononuclear cells stimulated with staphylococcal enterotoxin A (SEA) for 2-6 days significantly suppress [3H]thymidine incorporation and reduce the levels of interleukin 2 (IL-2) and interferon (IFN) in culture medium when added to fresh, polyclonally activated mononuclear cells.
Rigby et al. (1984)IL-2mononuclear cellsFurthermore, calcitriol suppressed interleukin-2 (IL-2) production by PHA-stimulated peripheral blood mononuclear cells in a concentration-dependent fashion.
Hopkins and Failla (1997)interleukin-2mononuclear cellsAlthough dietary copper (Cu) deficiency has been associated with decreased production of interleukin-2 (IL-2) by activated splenic mononuclear cells in rodent models, the basis for this relationship and its relevance for humans remain unknown.
Hopkins and Failla (1997)IL-2mononuclear cellsFinally, incubation of human peripheral blood mononuclear cells (PBMC) with 2,3,2-tet decreased mitogen-induced IL-2 production by 50% compared with untreated controls.
Majumder and Kierszenbaum (1995)interleukin-2mononuclear cellsWe show here that the marked inhibition of interleukin-2 production seen in hosts acutely infected with Trypanosoma cruzi can be reproduced in vitro by adding a T. cruzi suspension filtrate to cultures of activated human peripheral blood mononuclear cells.
Lin et al. (2000)interleukin-2HMNCThe inhibitory mechanisms may involve the blocking of interleukin-2 (IL-2) and interferon-gamma (IFN-gamma) production, since VC-ME suppressed IL-2 and IFN-gamma production of HMNC in a dose-dependent manner.
Tompkins et al. (1990)interleukin 2mononuclear cellsWe demonstrate in this report that SAP inhibits interleukin 2 (IL-2) biosynthesis by mitogen-stimulated peripheral blood mononuclear cells.
Iwaz et al. (1986)interleukin 2mononuclear cellsWe used the cholera toxin (CT), a cAMP elevating agent, to study the influence of this nucleotide on the production of interleukin 2 (IL-2) by human peripheral blood mononuclear cells stimulated by phytohemagglutinin and phorbol myristate acetate.