Viewing affirmative mentions of binding of IL2 (H. sapiens) in NK cells

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Voss et al. (1992)IL-2NK cellsExamination of gamma chain expression in post-IL-2 therapy NK cells, where only low levels of intermediate-affinity IL-2 binding were detectable, revealed that the gamma chain was associated with, on average, only 10-12% of the beta chains expressed on such cells.
Kehri et al. (1988)IL-2NK cellsScatchard analysis of 125I-IL-2 binding to purified NK cells revealed approximately 2,300 p70 binding sites per cell with an apparent dissociation constant of 200 pM, a value intermediate between the previously recognized high and low affinity forms of the human IL-2 receptor.
Umehara and Bloom (1990)IL-2LGLTherefore, these data clearly show that the IL-2-IL-2R beta interaction is responsible, and probably completely so, for the proliferative and cytolytic-promoting effects of IL-2 on LGL.
Vujanovic et al. (1995)IL-2NK cellsHuman NK cells can be separated into two functionally distinct subpopulations based on the ability to rapidly respond to IL-2 by adherence to solid surfaces.
Shaw et al. (1985)IL-2NK cellIL-2 responsiveness segregated with a non-E-rosetting fraction comprising 11% of postfractionation lymphocytes, and containing 94% of the recoverable NK activity, suggesting that IL-2 might operate directly upon the NK cell rather than through an accessory cell.
Shaw et al. (1985)IL-2NK cellIL-2 responsiveness segregated with a non-E-rosetting fraction comprising 11% of postfractionation lymphocytes and containing 94% of the recoverable NK activity, suggesting that IL-2 might operate directly upon the NK cell rather than through an accessory cell.
Escudier et al. (1994)IL2natural killer cellsImmunotherapy with interleukin-2 (IL2) and lymphokine-activated natural killer cells: improvement of clinical responses in metastatic renal cell carcinoma patients previously treated with IL2.
Rossi et al. (1994)IL-2NK cellWhereas IL-12 alone produced only modest enhancement of NK cell cytotoxicity, the combination of IL-2 and IL-12 was most effective in activating NK cell lysis of neuroblastoma cell lines.
Voss et al. (1992)interleukin 2natural killer cellsCharacterization of the interleukin 2 receptors (IL-2R) expressed on human natural killer cells activated in vivo by IL-2: association of the p64 IL-2R gamma chain with the IL-2R beta chain in functional intermediate-affinity IL-2R.
Brown et al. (1985)IL-2NK cellsWe conclude that the SF medium supplemented with IL-2 can be used as an alternative to FBS-containing medium with IL-2 for the growth of NK cells and is advantageous for the production of NKCF.
Condiotti and Nagler (1996)IL-2NK cellCampath-1G significantly inhibited binding of LGLs to tumor cells as well as resting and interleukin-2 (IL-2)-activated LGL and NK cell cytotoxic capabilites, which may be of clinical importance.
Panelli et al. (2002)IL-2-dependentNK cellsIn addition, three were associated with IL-2-dependent NK-cell activation: NK4, CD62 P-selectin, and galectin 1, as recently observed when purified NK cells were exposed to IL-2 (S.
Melki et al. (2010)IL-2NK cellsPurified NK cells were cultured at 106cells/ml for 48 hours either in the presence of suboptimal concentration of IL-2 (100 ng/ml) (Peprotech) to maintain their viability (referred as rNK), or were activated by a combination of PHA (10 g/ml) (Sigma) and rhuIL-2 (10 g/ml) (referred as aNK cells), before launching NK-DC coculture experiments.
deMagalhaes-Silverman et al. (2000)IL-2NK cellsAll patients developed neutropenic fevers, but the overall toxicity associated with the infusion of IL-2 (cohort 2) or IL-2 plus activated NK cells (cohort 3) did not differ from that observed in cohort 1.
LeFever et al. (1991)IL-2NK cellsIL-2 activated effector cells that bound to target cells also lysed them (i.e., non-lytic bystander lymphocytes did not influence the determination of kinetic parameters) in contrast to lysis mediated by unactivated NK cells.
Liang et al. (1988)IL-2natural killer cellsIn this study, the abilities of these IL-2 mutant proteins to activate natural killer cells and to induce interferon-gamma production have been evaluated, and the binding of these proteins to IL-2 receptors analyzed.
Allavena and Ortaldo (1984)IL 2LGLClones derived from purified human large granular lymphocytes (LGL) of three different donors were expanded in culture medium supplemented with interleukin 2 (IL 2).
London et al. (1986)IL 2NK cellsInteraction of IL 2 with the Tac IL 2 receptor expressed on activated NK cells is necessary to maintain continued growth of these cells.
Yamada et al. (1991)IL-2-culturedLGLThe cytotoxic efficiency and the binding affinity of IL-2-cultured LGL in off-therapy ALL patients was not decreased.
Matos et al. (1993)IL-2NK cellThe potentiating effect of SF on NK cell proliferation was dependent on IL-2 binding to the high affinity IL-2R, and was blocked by a monoclonal antibody directed against the c-kit receptor.
Dybkaer et al. (2007)IL2NK cellsIn conclusion, our study demonstrated gene expression profiles associated with IL2 induced increased cytotoxicity, changes in chemokines, cytokines and adhesion properties, enhanced pro-inflammatory and innate immune response and changes in signaling pathways in NK cells.
Krishnaraj (1992)IL2NK cellsThis down-regulation can be partially overcome by IL2 that up-regulates NK cells.
Ye et al. (1995)IL-2NK cellsIn this report we present evidence that the cell line NK3.3 derived from human NK cells, responds to both IL-2 and IL-12, as measured by increases in IFN-gamma and granulocyte-macrophage colony-stimulating factor (GM-CSF) cytoplasmic mRNA and protein expression.
Carson et al. (1994)IL-2NK cellsThe CD56bright subset of human NK cells constitutively expresses the high affinity IL-2R and exhibits a brisk proliferative response after the binding of picomolar amounts of IL-2.
Jewett et al. (2010)IL-2NK cellsThis was similar to the profiles which we had seen when NK cells were treated with IL-2 and anti-CD16 antibody in which significant decrease in cytotoxicity of NK cells could be observed in parallel with increased secretion of IFN-?
Rambaldi et al. (1986)interleukin 2LGLThe increased numbers of LGL in these patients had little or no natural killer activity, mediated antibody-dependent cellular cytotoxicity, and were induced to kill tumour lines after culture for 3 days with interleukin 2 (IL-2).
My?liwska et al. (2000)IL2NK cellsSince the CD25 molecule constitutes a subunit of the high affinity receptor, binding IL2 to immunocompetent cells, its increased expression on NK cells of low NK responders would enable them to bind even low amounts of the endogenous IL2 available in this group of the elderly.
Cordero et al. (1992)IL-2NK cellFinally our data suggest that the effect of ProT alpha enhancing the NK cell cytotoxicity appears to involve enhancement of p70 IL-2R expression and internalization of IL-2, and was independent of cell proliferation.
Millman et al. (2008)IL-2NK cellOur results strongly indicate that glutathione in combination with IL-2+IL-12 augments NK cell functions, leading to control M. tuberculosis infection.
Penafuerte et al. (2009)IL-2NK cellIn the present work, we have found that human ortholog of the GM-CSF/IL-2 fusion protein (a.k.a. hGIFT2) induces robust NK cell activation ex vivo with significant secretion of RANTES and a 37-fold increase in IFNgamma production when compared with either IL-2 or GM-CSF single cytokine treatment or their combination.
Ben Aribia et al. (1989)IL-2NK cellsIL-2-binding sites expressed on purified circulating NK cells and high density T lymphocytes were enumerated in 125I-IL-2 binding assays and analyzed by autoradiography after chemical cross-linking of IL-2 to the cells.
Minty et al. (1993)IL-2large granular lymphocytesMoreover, it synergizes with IL-2 in regulating interferon-gamma synthesis in large granular lymphocytes.
Allavena et al. (1985)IL-2LGLLGL subpopulations were also tested in a limiting dilution assay (LDA) for their capacity to proliferate in medium supplemented with interleukin 2 (IL-2) and to develop NK-like cytotoxic activity.
Hori et al. (1988)IL-2LGLFurthermore, LGL from the four patients proliferated in response to higher concentrations of IL-2 and these responses were not inhibited by an excess amount of anti-Tac antibody. 125I-Labeled IL-2 cross-linking studies performed in two cases revealed the predominant expression of an IL-2 binding molecule with an estimated Mr of 70,000 to 75,000.
Hori et al. (1988)IL-2LGLLarge granular lymphocytes (LGL) from four patients with abnormally expanded LGL in the peripheral blood were studied regarding their receptor for IL-2.
Hori et al. (1988)IL-2LGLThese data strongly suggested that the IL-2R expressed on LGL is functional and identical to the p70, a novel IL-2 binding peptide that has been recently identified and speculated to form the high affinity IL-2R in association with the p55.
Chalifour et al. (2004)interleukin-2NK cellsThese proteins induce interferon-gamma (IFN-gamma) production by NK cells and synergize with interleukin-2 (IL-2) and proinflammatory cytokines in PAMP-induced activation.
Csipo et al. (1998)IL-2NK cells(ii) NK cells exposed to Fas+ TC completely recover the Fas lytic pathway and the ability to fragment DNA via the Fas/Fas ligand when incubated in medium supplemented with IL-2 for 18 h.
Azzoni et al. (1995)IL-2NK cellFc gamma R stimulation does not induce NK cell proliferation, and inhibits that induced by IL-2, but not by IL-12, as measured by [3H]TdR incorporation, without affecting entrance or progression through cell cycle.
Tseng et al. (2010)IL-2NK cellsInduction of NK cell anergy by anti-CD16 antibody, even though abrogated the ability of IL-2 treated NK cells to lyse HEp2 cells, the same treatment resulted in a significant induction of IFN-?
Tsudo et al. (1987)p75 IL-2LGLIn the present study, using cross-linking methodology, we found that normal LGL and leukemic LGL from all individuals tested expressed the p75 IL-2 binding peptide but did not express the Tac peptide.
Chouaib et al. (1988)IL-2LGLOur results suggest a functional interaction between IL-2 and TNF on LAK precursors, which results in a reduction of the IL-2 concentration required for differentiation of LGL into LAK killers.
Hombach et al. (2005)IL2NK cellsSimultaneous targeting of IL2 and IL12 to Hodgkin's lymphoma cells enhances activation of resting NK cells and tumor cell lysis.
Keever et al. (1989)IL-2NK cellsWe have examined the effect of IL-3 and IL-4 on the differentiation of NK cells from their marrow-derived precursors and have further examined the interactions of these cytokines with IL-2 and IL-1.
Dukovich et al. (1987)IL-2large granular lymphocytesIn this study, we report the identification of a second human IL-2 binding protein that (1) has an Mr of approximately 70K, (2) lacks reactivity with the anti-Tac antibody, (3) binds IL-2 with intermediate affinity and (4) is present on the surface of resting T cells, large granular lymphocytes (natural killer cells), and certain T and B cell lines in the absence of the Tac antigen.
Voss et al. (1990)IL-2natural killer cellsIncreased expression of the interleukin 2 (IL-2) receptor beta chain (p70) on CD56+ natural killer cells after in vivo IL-2 therapy: p70 expression does not alone predict the level of intermediate affinity IL-2 binding.
Klokker et al. (1997)interleukin 2NK cellIn support, NK cell activity boosted with interferon-alpha and interleukin 2 rose in parallel with unboosted NK cell activity and NK cell concentration and activities returned to the baseline level after 105 min.
Lehmann et al. (2001)IL-2NK cellsUsing the NK-resistant tumour cell lines ML-2, MONOMAC-1, RPMI and L540Cy, we demonstrated that activation of NK cells with interleukin 2 (IL-2) and IL-12 resulted in significant lysis of these tumour targets.
Riederer et al. (2010)IL-2NK cellsA movie showing the interaction of TKD/IL-2-activated NK cells and ECs indicates that a bulk of NK cells actively migrates towards ECs.
Wang et al. (2000)IL-2NK cellsIn this study, we examine the interaction between IL-2 and the signaling events induced by IL-12 in NK cells.
Wei et al. (1993)IL-2NK cellsIn addition to T cells, NK cells, B cells, and monocytes, we provide new evidence that human polymorphonuclear neutrophils (PMN) can be functionally activated by IL-2 via binding to IL-2R beta expressed on the cell surface.
Yang et al. (1994)IL-2NK cellsHowever, the NK cells normally responded to interleukin 2 (IL-2).
Melder et al. (2005)IL-2NK cellsMETHODS: The effect of Albuleukin on lymphocyte proliferation, IL-2 receptor binding, and release of IFN-gamma from human NK cells were examined in vitro.
Wang et al. (2000)IL-2NK cellsThese findings provide a molecular framework to understand the interaction between IL-2 and IL-12 in NK cells, and suggest strategies for improving the effectiveness of these cytokines in the immunotherapy of cancer.
Mizrahi et al. (2007)IL-2NK cellsThe expression of CD100 is up-regulated on the surface of NK cells after their activation with IL-2, IL-12 and IL-15 (figure 1), implicating CD100 as activation marker for NK cells.
Adolf (1985)interleukin 2natural killer cellIts effects on the immune system, including enhancement of membrane antigen expression, macrophage activation, effects on B lymphocytes, enhancement of natural killer cell activity and the interaction between IFN-gamma and interleukin 2 are discussed.
Torigoe et al. (1992)IL-2natural killer cellRecently, interleukin 2 (IL-2) has been shown to induce increased activity of the p56lck protein-tyrosine kinase (PTK) in T-cell and natural killer cell lines, and evidence for a direct interaction between the p75 subunit of the IL-2 receptor (IL-2R) and this src-family kinase has been reported.
Gismondi et al. (2004)IL-2NK cellsTreatment with interleukin-2 (IL-2) corrected the functional defects of NK cells by affecting their ability to bind to sensitive target cells and to accumulate F-actin.
Brunda et al. (1986)recombinant interleukin-2natural killer cellInteraction of recombinant interferons with recombinant interleukin-2: differential effects on natural killer cell activity and interleukin-2-activated killer cells.
Arancia et al. (1995)interleukin-2-activatednatural killer cellsNoninhibitory binding of human interleukin-2-activated natural killer cells to the germ tube forms of Candida albicans.
Sadi et al. (2008)IL-2NK cellsAutologous NK cells were sorted and either kept unstimulated in the presence of suboptimal concentration of IL-2, or activated by a combination of PHA and IL-2.
Matera (1997)IL-2NK cellIn particular, assignment of PRL to the T helper 1 phenotype is proposed, based on its ability to enhance NK cell function, activate the interferon-regulated factor (IRF-1) transcription factor and to interact with or generate IL-2 and IFN gamma.