Viewing negative mentions of gene expression of IL2 (H. sapiens)

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Nutman et al. (1987)interleukin 2In marked contrast, when antigen-induced lymphokine production was examined, most patients with microfilaremia were unable to produce either interleukin 2 (IL-2) or gamma-interferon (i.e., were nonresponders), and the few who could (hyporesponders, generally with quite low microfilaremia levels) did so at levels significantly less than those of patients with elephantiasis, all of whom showed strong responses to parasite antigen.
Toossi et al. (1989)IL-2Leu 11-enriched cells did not express high affinity IL-2 receptors nor did they deplete IL-2 activity from culture media.
Manger et al. (1986)IL-2After treatment with 5-azacytidine, HUT 78 cells produced maximal levels of IL-2 in response to PMA alone without requiring [Ca++]i increasing stimuli.
Liu and Rosenberg (2001)IL-2PBMCs similarly transduced with a control vector did not produce IL-2 and failed to proliferate in the absence of IL-2.
Packard (1990)IL-2However, IL-2 is not normally synthesized by solid tumor cells.
Visani et al. (1987)IL2Noninduced cells did not express IL2 receptors.
Bettens et al. (1984)IL 2Furthermore, anti-Tac can inhibit the mitogenic signal given by endogenous IL 2, but not by in situ produced IL 2, an observation of importance to further investigations of the mechanisms by which IL 2 interacts with specific receptors to elicit proliferation.
Philip et al. (1996)IL-2Transfections with the non-AAV plasmid containing the identical expression cassette as the AAV plasmid induced IL-2 expression in the tumor cell line but failed to produce IL-2 in primary tumor cells.
Miedema et al. (1985)IL2Moreover, the T8+ cells produced IL2, despite their failure to help Ig synthesis.
Zicari et al. (1995)IL-2Immunoreactive IL-2 was detected only in AF samples obtained from women of Groups 1 and 3; the higher concentration was found in Group 1 samples; IL-2 was not detected in AF samples from women of Group 2.
Salmon et al. (1988)IL-2The helper cells produced no detectable IL-2 and proliferated poorly.
Teodorczyk-Injeyan et al. (2008)IL-2Seven (2 each in VC and SMT-NC and 3 in SMT-C group) of 76 subjects investigated in this study demonstrated no detectable IL-2 synthesis in unstimulated PBMC cultures both prior to and post-intervention.
Dohlsten et al. (1988)IL2While the CD4+45R- subset produced maximal amounts of IL2 within 24 h and IFN-gamma within 72 h, the CD4+45R+ subset produced no IL2 within 24 h and merely marginal amounts of IFN-gamma as assayed after 24 to 96 h.
Miller et al. (1986)IL 2Although these cells have been activated to an IL 2R+ stage, most of them are unable to produce detectable IL 2 unless they are cultured together with the original, activating antigen.
Schiphorst et al. (1999)IL-2IL-2 could not be detected in haemo- or peritoneal dialysates.
Miller and Olds (1987)IL-2Cultures from children with either active lupus or the pneumonia of cystic fibrosis produced very little IL-2, but cultures from children with arthritis produced apparently normal amounts.
Hori et al. (1986)IL-2Leukemic cells from 7 ATL patients examined showed no or very poor proliferative response to IL-2 though they expressed IL-2 receptors without any stimulation.
Morita et al. (1992)IL-2IL-2 and IL-4 were not detected in any cases tested.
Kumagai et al. (1988)IL-2Competence is defined as the ability to subsequently proliferate in response to exogenously added IL-2 or PDB in the second phase of the culture, but not to synthesize IL-2 or proliferate without these additions.
Dohlsten et al. (1988)IL-2In contrast, the Leu 3+18+ subset produced no IL-2 after 24 h, and maximal amounts of IL-2 no sooner than 48 h of culture, and only small amounts of IFN-gamma during the entire culture period of 96 h.
Pawelec et al. (1982)IL2A second, JM, failed to produce any IL2, while the third, JHAN, produced intermediate levels.
Kato et al. (1997)IL-2They did not produce IL-2 by stimulation with PHA and phorbol myristate acetate (PMA).
Kirkpatrick et al. (1985)IL-2Asymptomatic heterosexual and homosexual subjects produced comparable amounts of IL-2, but 8 of 11 AIDS patients with opportunistic infections and two of three AIDS patients with both opportunistic infections and Kaposi's sarcoma failed to produce detectable amounts of IL-2; all seven AIDS patients with only Kaposi's sarcoma produced IL-2.
Kirkpatrick et al. (1985)IL-2In addition, three of eight AIDS patients who did not produce IL-2 produced supernatants that inhibited growth of IL-2-dependent cells in the presence of IL-2.
Hovdenes and Egeland (1987)IL-2IL-2 was not detected in unstimulated cell-culture supernatants from any of the four RA patients tested, but it was present in small amounts in supernatants of unstimulated cultures derived from three of seven JRA patients studied.
Tsutsui et al. (1991)IL-2However, the other four helper-type clones did not produce IL-2 by such stimulation, although they continued the production of IL-4.
Siegel et al. (1987)IL-2Yet, prior to activation, these cells do not express IL-2 receptors recognized by monoclonal antibodies to the Tac antigen.
Pinkerton et al. (2008)IL2Genes that were not expressed included: (1) the immunoregulatory lymphokines (IL2-IL5), IL17 and IL17B; (2) IL10 and other members of the IL-10 family of anti-inflammatory cytokines (IL19, IL20, IL22 and IL24); and (3) TNF and RANKL.
Ge et al. (2004)IL-2Investigation of IL-15 receptor expression using RT-PCR showed that IL-15Ralpha and IL-2Rbeta chains but not IL-2Ralpha or gamma chain were constitutively expressed in these cells.
Bykovskaia et al. (1999)IL-2Isolated CD34+ cells did not express IL-2R subunits but after 2-3 days in culture with IL-15, they were found to express IL-2Rgamma.
Paglieroni et al. (1988)IL2Cells from patient 1 coexpressed CD5 (leu-1), CD19 (Leu-12), CD20 (B1), and HLA-DR; they were CD10 (J5), CD21 (B2), CD22 (Leu-14), CD25 (IL2-R1), PCA-1, surface, and cytoplasmic Ig negative.
Casoli et al. (2000)IL-2Endogenous IL-2 was not produced, whereas IL-6 and TNF-alpha were secreted at levels compatible with their known ability to up-regulate HIV-1 expression.
Loertscher et al. (1988)interleukin 2In the presence of Ts, most MLR responder cells did not express interleukin 2 receptors, although a small minority of cells constantly escaped Ts inhibition.
Vyth-Dreese and de Vries (1983)IL-2These continuously proliferating T-CLL cells failed to produce IL-2 constitutively.
Kumaki et al. (1995)IL-2Using Epstein-Barr virus-transformed B-lymphoblastoid cell lines (B-LCLs) established from two unrelated XSCID patients, we could show that neither expressed the IL-2 receptor gamma chain on the cell surface.
Fujii et al. (1988)IL-2However, such a culture in the medium with autoserum showed no such IL-2 production or cytotoxicity.
Gogacz et al. (1998)IL-2The level of IL-2 was not detectable in 2 cases from women with UI and 5 cases from reference group.
Castes et al. (1988)IL-2Patients with DCL, who are characterized by their defective cell-mediated immune response to leishmanial antigen, failed to express the IL-2 receptor and did not produce IFN-gamma when exposed to the antigen but did so when stimulated by phytohemagglutinin M.
Urashima et al. (1992)IL-2JK-T1 was independent of interleukin 2 (IL-2) because of little production of IL-2, little IL-2 receptor (CD25) on the surface, and no response to exogenous IL-2.
Grimm et al. (1995)IL-2Eight showed no cytokine staining, 2 expressed IL-2, and 3 expressed IL-4 and IFN-gamma.
Ho et al. (1988)IL-2Our results showed that no detectable IL-2 was produced and no IL-2R were expressed by PBMC in response to MA during the acute infection.
Allavena et al. (1985)IL-2The ability to produce IL-1 or IL-2 was not usually found within the same clones following PHA and LPS stimulation, respectively; however two clones produced both IL-1 and IL-2 when stimulated in different experiments, but not at the same time.
Vercoulen et al. (2009)IL-2As a result Treg do not produce IL-2, and even may act as a ‘sink’ for IL-2.
Euhus et al. (1997)IL-2RESULTS: IL-2 was never detectable in the untransduced cultures, but it peaked at 5.0-1,324.8 ng/ml in the transduced cultures.
Garcia Domingo et al. (1988)IL-2These results indicate that PS was interfering with IL-1 activity by rendering IL-2-producer cells unable to synthesize IL-2 and subsequently to proliferate.
Wood et al. (1984)IL-2These results indicate that failure to produce IL-2, a powerful mediator of cellular immune responses, is an important mechanism underlying the defective cell mediated immunity seen in burn patients.
Williams et al. (1987)IL-2The purified chimeric toxin is shown to selectively inhibit protein synthesis in IL-2 receptor bearing targeted cells, whereas cell lines which do not express the IL-2 receptor are resistant to IL-2-toxin action.
Cignetti et al. (1994)IL2CONCLUSIONS: The results of this study demonstrate that the IL2 gene can be productively transduced into human myeloid and lymphoid leukemic cells without modifying their phenotypic and proliferative properties and that this transduction leads to a reduced or abrogated in vivo tumorigenic potential.
Lee et al. (1988)interleukin-2The assay is simple, reproducible and specific in detecting different batches of human interleukin-2 of natural as well as recombinant origin, but not detecting recombinant murine interleukin-2.
Arya et al. (1984)TCGF messenger RNAIt is now reported that cells infected with this virus also lack detectable TCGF messenger RNA (less than one copy per cell) and thus do not produce their own growth factor.
Woerly et al. (1996)IL-2These effects are not secondary to an IL-2-mediated CD25 up-regulation, as rapamycin inhibits neither IL-2 synthesis nor IL-2-induced CD25 mRNA.
Maccario et al. (1987)interleukin-2However, all infected patients tested showed a very low or absent phytohaemagglutinin induced interleukin-2 production regardless of age and clinical conditions, while concanavalin A-induced interleukin-2 production was within the normal range.
Zhang et al. (1995)IL-2Following primary purification, IL-2(60)-PE40 was shown to be very toxic to IL-2 receptor-positive cells but non measurable effect on the cells lacking IL-2 receptors.
Ishiyama et al. (1989)IL-2Although 3 patients without M protein did not respond to IL-2, they did respond to the supernatant, and they proliferated but expressed no IL-2 receptors.
Clark et al. (2003)IL-2CONCLUSION: One course of high-dose bolus IL-2, though feasible, did not produce the ambitious clinically meaningful benefit anticipated when administered postoperatively to patients with resected high-risk RCC.
Willenborg et al. (1988)IL-2Cells treated with 300 ng/ml are unable to replicate in vitro in response to concanavalin A stimulation, nor did they produce interleukin-2 (IL-2) following stimulation.
Saltzman et al. (1997)IL-2We previously have demonstrated that the thi 4550(pIL-2) produces biologically active IL-2 and that a single gavage feeding of 10(7) thi 4550(pIL-2) significantly reduced the number of hepatic metastases when compared with animals fed salmonella lacking the IL-2 gene or nontreated controls.
Goldstein et al. (1992)IL-2We than analyze the case in which heterodimers exist in the absence of IL-2 and the case in which no preformed heterodimers exist.
Wang et al. (1991)IL-2This effect was not mediated through decreased cAMP, since the forskolin-enhanced cAMP level was not influenced by IL-2, IL-1, with or without hCG, did not show any effect on progesterone production during either 24 or 48 h of cell culture.
Hjelm Skog et al. (2001)IL-2Before treatment, no IL-2 antibodies were detected in any of the patients.
Starkebaum et al. (1991)IL-2However, no IL-2 was detectable in supernatant from the cell line, while antibodies to IL-2, or to the IL-2 receptor alpha or beta chains did not inhibit cell growth.
Yang et al. (2003)IL-2IL-2 was more clinically active at maximal doses, although this did not produce an overall survival benefit.
Kahaleh and LeRoy (1989)Interleukin-2Interleukin-2 was found in sera from 41 of 47 patients with scleroderma (204 +/- 356 U/mL, mean +/- SD), in 9 of 20 patients with rheumatoid arthritis (2.04 +/- 5.16), and in none of 14 matched control subjects.
Weller et al. (1992)IL-2Results for IL-1, IL-2, and IL-4 were always negative. sIL-2R and IFNy were detected occasionally but not associated with specific malignant neoplasms.
Paglieroni et al. (1988)IL2They were negative for CD10 (J5), CD25 (IL2-R1), and PCA-1.
Tsubai et al. (1987)interleukin 2In contrast to interleukin 2 receptors expressed on both ATL cells and normal phytohemagglutinin-stimulated blasts, this antigen was not expressed on the latter cells.
Suzuki et al. (1986)interleukin 2Regardless of this specific DNA replication, the AMLR generated no production of interleukin 2 (IL-2) and interferon gamma (IFN-gamma), both of which could be found in the allogeneic (allo) MLR.
Petersen et al. (2002)IL-2In this setting the CT.h4S bioassay detects 5 pg/ml of human recombinant IL-4 with no detection of IL-2 in concentrations below 500 pg/ml.
Rocha-Zavaleta et al. (2004)IL-2Interestingly, endogenous IL-2 is not produced by these cells, suggesting the activation of IL-2Rbetagamma by an alternative mechanism.
11365845IL-2Four studies on Interleukin-2 (IL-2) ascertained that IL-2 produced significant increases in CD4+ cell counts, without increasing viral load, and produced fewer side effects when administered by subcutaneous injections.
Clerici et al. (1994)IL-2Four different patterns of TH function were observed in HD patients: (1) IL-2 production in response to all of the stimuli (40%); (2) IL-2 production in response to ALLO and PHA but not to FLU (26%); (3) IL-2 production in response to PHA alone (19%); and (4) failure to respond by IL-2 production to any of the three of the stimuli (15%).
Inaba and Geiger (2006)IL-2Upon re-stimulation the refractory cells produce little IL-2 and show diminished proliferation.
Cozzolino et al. (1987)IL-2Under these culture conditions, these cells did not produce IL-2, and no proliferation was observed.
Pohling et al. (2010)IL-2Stronger responses reflected higher numbers of CD8+CD45RA- effector memory cells producing IFN-gamma, but not IL-2.
Mori et al. (1993)IL-2An HTLV-I carrier who developed CD4+ T-CLL expressed the IL-2 receptor beta chain alone without expressing the alpha chain.
Goey et al. (1995)IL-2Intrapleural IL-2 levels were high (> 20,000 IU ml-1) at levels E and F, while serum levels in most patients were not or barely detectable (< 3-30 IU ml-1).
Szekanecz et al. (1994)IL-2Human atherosclerotic abdominal aortic aneurysms produce interleukin (IL)-6 and interferon-gamma but not IL-2 and IL-4: the possible role for IL-6 and interferon-gamma in vascular inflammation.
Szekanecz et al. (1994)IL-2Neither AAA nor NL aortic tissues produced IL-2 or IL-4 in the same culture system.
Vercoulen et al. (2009)IL-2IL-2 and IL-7 overcome suppression, without influencing apoptosis
Osusky et al. (2008)IL2Fusion of interleukin 2 (IL2) to the N terminus of R336A-aerolysin results in a hybrid that has little or no activity against cells that do not have an IL2 receptor because it cannot bind to the GPI-AP on the cells.
Osusky et al. (2008)IL2Cell death occurs at picomolar concentrations of the hybrid, whereas the same cells are insensitive to much higher concentrations of R336A-aerolysin lacking the IL2 domain.
Duprez et al. (1988)IL2Although the half-life of IL2 receptors is very short in the absence of IL2, t 1/2 approximately 65 min, suggesting that these receptors are constantly endocytosed, it can still be reduced to t 1/2 approximately 25 min when the receptors are saturated with ligand.
Ferry et al. (1990)IL-2All three decidual cell populations proliferated in response to recombinant human interleukin-2 (rIL-2), but none produced detectable levels of IL-2 in culture.
Ojima et al. (2005)IL-2The infected culture continued to grow for 7 days even in the absence of IL-2.
Lansigan et al. (2010)IL2Interestingly, although IL2 was required for their growth and survival, Tregs did not produce IL2 themselves.18 Since CTCL is considered by some to be a malignant proliferation of T-regs,19 the rationale behind targeting CD25 is even more robust.
Owen-Schaub et al. (1988)IL-2Neither TNF-alpha nor -beta is able to induce LAK function in the absence of IL-2.
Katano et al. (1994)IL-2By using a sensitive polymerase chain reaction technique, we demonstrated that Breast M25-SF but not Breast M expressed IL-2 mRNA, and IL-2 secretion from Breast M25-SF but not Breast M was also confirmed by radioimmunoassay.
Smith (2006)IL2Even in the absence of IL2, they found low affinity binding of the ?
Honda et al. (1998)interleukin 2They produced interferon (IFN)-gamma, interleukin 2 (IL-2) and tumour necrosis factor (TNF)-beta but not IL-4.
Brown et al. (1982)TCGFIn the absence of TCGF, the cells were incapable of traversing the cell cycle and became arrested in G1.