Viewing affirmative mentions of binding of cd3g (X. laevis) in T cells

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Alroy et al. (2005)CD3T cellThe antibodies commonly used to recognize CD3 are directed against the epsilon-subunit of the T cell receptor.
Mezzetti et al. (1991)CD3T cellsECs restored mitogen-induced DNA synthesis and DNA synthesis triggered by CD3 cross-linking in T cells, as did pulmonary macrophages, and this was unrelated to HLA-DR expression.
Mezzetti et al. (1991)CD3T cellThe ability of promoting T cell proliferation after CD3 cross-linking was, in part, due to the secretory products of ECs, since their supernatants were also effective.
Blasini et al. (1998)CD3T lymphocytesIt is possible that the pattern of downstream biochemical signals triggered by TCR/CD3 ligation may be altered in T lymphocytes from patients with SLE, thus leading to abnormal distal cell responses.
Roosnek and Lanzavecchia (1989)CD3T cellsWe used a purified bispecific antibody (Ab) against CD3 and an ovarian carcinoma (OVCA) antigen to ask whether the binding of a monovalent ligand to CD3 can induce triggering of T cells.
Anderson et al. (1988)CD3T cellA smaller enhancement was induced when CD3 was crosslinked with several other functionally relevant T cell surface molecules.
Vidémont and Pin (2010)CD3T cellsFor the first time, immunohistochemistry was used to show a closed association between CD3(+) T cells and caspase-3 stained keratinocytes, consistent with a keratinocyte apoptosis by epidermal-infiltrating T cells.
Kretz-Rommel and Rubin (1997)CD3T cellsAddition of procainamide-hydroxylamine, but not procainamide or its further oxidation products during anergy induction by CD3 engagement, caused a dose-dependent recovery of the capacity of T cells to proliferate and secrete IFN-gamma upon subsequent Ag challenge.
Link and Weiner (1993)CD3T-cellBispecific monoclonal antibodies (bsabs) recognizing both CD3 and a tumor antigen can redirect T-cell-mediated cytotoxicity toward cells bearing that antigen.
Shivnan et al. (1992)CD3T cellPrevious studies have shown that co-ligation of the CD45 and CD3 antigens inhibits CD3-induced tyrosine phosphorylation, IP production, calcium signals and T cell proliferation.
Matis et al. (1987)TCR gamma deltaT-cellCritical to understanding the function of this T-cell subset is the identification of the ligand(s) recognized by TCR gamma delta.
Anderson et al. (1988)CD3T lymphocyteT lymphocyte proliferation can be triggered through interactions with either CD3:Ti, the target of antigen-specific activation, or CD2, the target of an antigen-independent activation pathway.
Sjöberg et al. (2002)CD3TALWe investigated whether the described immune evasion of Epstein-Barr virus (EBV)-infected malignant Hodgkin and Reed-Sternberg (HRS) cells in Hodgkin's disease (HD) is paralleled by a disturbed expression of the signal transduction molecule zeta associated with CD3 and CD16 in tumour-associated T lymphocytes (TAL).
Weiner and De Gast (1995)CD3T-cellsBsAbs that recognize CD3 and a number of antigens on malignant B-cells have been shown in vitro to be capable of retargeting T-cells.
Volc-Platzer et al. (1993)gamma deltaT-cellMonoclonal antibodies (moAbs) that recognize common or variable determinants of the gamma delta T-cell receptor (TcR) were used to assess gamma delta T-cell distribution on biopsy specimens and/or peripheral blood leukocytes (PBL) from 30 patients suffering from chronic cutaneous lupus erythematosus (CCLE).
Brischwein et al. (2007)CD3T cellsA particular challenge of bispecific antibody constructs that recognize the invariant CD3 signaling complex is a controlled polyclonal activation of T cells that, ideally, is exquisitely dependent on the presence of target cells.
Lanier and Weiss (1986)CD3T-cellThese lymphocytes may express the product of the T-cell antigen receptor gamma (TCR-gamma) gene, rather than the alpha/beta heterodimer, in association with CD3.
Anderson et al. (1988)CD3T lymphocyteCrosslinking CD3 with CD2 using sepharose-immobilized antibodies enhances T lymphocyte proliferation.
Atkuri et al. (2005)CD3T cellThus, we conclude that the influence of oxygen levels on proliferation depends on the stimulus, and, most importantly from the standpoint of immune responses, culturing cells at atmospheric rather than physiologic oxygen levels results in significantly increased proliferation responses to the CD3/CD28 crosslinking, a proliferation stimulus commonly used to mimic T cell antigen receptor signaling.
Schlegelberger et al. (1990)CD3T-cellThe aberrant mitoses showed a positive reaction with the monoclonal T-cell antibody Leu 4 (CD3).
Fyfe et al. (1994)CD3T-cellBoth CD3 and T-cell receptor cross-linking have been associated with lymphocyte activation and expression of the LFA-1 adhesion ligand, which leads to adhesion to intercellular adhesion molecule 1 immobilized on artificial surfaces.
Shivnan et al. (1992)CD3T cellsThe binding of agonistic monoclonal antibodies (mAb) to the CD3 antigen in T cells induces a rapid increase in tyrosine phosphorylation, inositive phosphate (IP) production, a rise in intracellular calcium and protein kinase C (PKC) activation.
Kappes and Tonegawa (1991)CD3T cellsT-cell antigen receptor (TCR) heterodimers of both the alpha beta and gamma delta types are expressed at the surface of T cells only in association with a complex of invariant chains called CD3.
Wong et al. (1992)CD3T-cellComparative studies of isolated CD3: CD8, CD3: CD3, and monovalent CD3 binding on CD8+ T-cell activation: model of progressive T-cell receptor aggregation synergism.
Cifone et al. (1995)CD3T cellArachidonic acid (AA) release was observed following T cell receptor (TCR)/CD3 complex cross-linking in different tumor T cell lines as well as on purified peripheral T cells in vivo.
Cifone et al. (1995)CD3T cellImportantly, evidence for further metabolism of released AA was obtained, since synthesis and release of cysteinyl leukotrienes (CLT), but not of leukotriene B4 or cyclooxygenase products, could be detected by radioimmunoassay in different T cell lines and peripheral blood T cells following TCR/CD3 cross-linking.
Shivnan et al. (1992)CD3T cellIn this study co-ligation of the CD3 and CD45 antigens with precisely constructed bispecific mAb did not inhibit CD3-induced T cell proliferation, IP production, calcium signals, diacylglycerol production or PKC activation.
Linn et al. (2009)CD3T-cellBoth the CD3(+) CD56(+) and CD3(+) CD56(-) subsets, though known to kill autologous and allogeneic targets to a comparable degree and therefore non-major histocompatibility complex (MHC)-restricted, nevertheless require the presence of the MHC molecule on the target, which interacts with their CD3-T-cell receptor complex.
Kim et al. (2007)CD3T-cellsWe recently reported that an affinity matured single-chain (scFv) anti-monkey CD3 antibody, C207, had increased binding to T-cells and increased bioactivity in a diphtheria toxin (DT)-based biscFv immunotoxin compared with the parental antibody, FN18.
Leitenberg et al. (1995)CD3T cellsIn this report, we identify distinct patterns of calcium mobilization in CD4+ T cells following the antibody-mediated cross-linking of either CD3 or CD4, or following the cross-linking of both CD3 and CD4 simultaneously.
Leitenberg et al. (1995)CD3T cellThis sustained increase in intracellular calcium concentration is also seen following physiologic cross-linking of CD3 and CD4 after T cell interaction with specific antigen and antigen-presenting cells.
Briscoe et al. (1992)CD3T cellMean scores (0 to 3+) for endothelial VCAM-1 expression increased from 0 (CD3+ score, 0) to a mean score of 2.25 in association with CD3+ T cell infiltrates (CD3+ score, 3).
Müller et al. (2006)CD3T cellsConsistent with their inability to activate Cdc42 and Rac1 in response to the ligation of CD3/CD28, T cells exposed to MV fail to acquire a morphology consistent with spreading and lamellopodia formation.
Nikolai et al. (1998)CD3T-cellSimultaneous engagement of CD3 and CD2 very potently stimulated T-cell migration, resulting in the recruitment of previously sessile cells (about 24% of the total population was additionally recruited) as well as an increase in the mean duration of active locomotion.
Geisler (1992)CD3T cellTransfection of the wild-type CD3-gamma gene into JGN reconstituted expression of functional Ti/CD3 complexes, and analysis of T cell lines producing different amounts of CD3-gamma indicated that CD3-gamma and CD3-delta competed for the binding to CD3-epsilon.
Testi et al. (1989)CD3T cellsThe induction of Leu 23 strictly correlated with the extent of CD3/TCR cross-linking on the surface of T cells.
De Jonge et al. (1995)CD3T cellsThe protein possesses a dual specificity: the single-chain FvB1 portion is directed to the Idiotype of BCL1 lymphoma cells, the single-chain Fv2C11 moiety binds to the CD3 marker on T cells.
Park et al. (2005)CD3T cellsA polyclonal rabbit antiserum that reacts with the CD3 marker on human T cells also reacted with Japanese flounder CD3epsilon.
Linette et al. (1988)CD3T cellThe binding of antigen or monoclonal antibody to the T cell receptor for antigen or the closely associated CD3 complex causes increases in the concentration of intracellular ionized calcium and subsequent cell proliferation.
Takemura et al. (2000)CD3T cellsAs a model, a bispecific diabody binding to adenocarcinoma-associated antigen MUC1 and to CD3 on T cells was studied.
Monserrat et al. (2009)CD3T cellA different kinetic pattern of T cell subset involvement is observed in surviving and nonsurviving patients, with lower numbers of circulating CD3+CD8+CD28+ and CD3+CD8+CD62L+ associated with a better disease outcome.
Hara et al. (1991)CD3T-cellThese data suggest a discrete association of CD3 and CD4 molecules in T-cell stimulation, acting through the autologous mixed lymphocyte reaction and the CD3/T-cell receptor complex.
Hara et al. (1991)CD3T-cellDiscrete association of CD3 and CD4 molecules in T-cell stimulation acting through the autologous mixed lymphocyte reaction and the CD3/T-cell receptor complex in human autoreactive T-cell clones.
Davis et al. (1989)CD3T cellsAfter modulation of 38.1 bound CD3, T cells were markedly inhibited in their capacity to respond to PHA.
Hamawy et al. (2001)CD3T cellsMETHODS: Purified normal rhesus monkey T cells were incubated with unconjugated FN18 or conjugated FN18-CRM9 and examined for differences in antibody binding, tyrosine phosphorylation, and CD3 internalization.
Dasgupta et al. (1990)CD3T cellThough surface modulation and immunoprecipitation experiments do not indicate any physical association between these two types of molecules on the T cell surface, capping studies show that cross-linking of class I Ag induces an association of these Ag with CD3.
Dasgupta et al. (1990)CD3T cellBased on these observations we propose that during antigen presentation M phi (or Ag-presenting cells) and T cells, besides interacting via peptide--class II Ag/CD3--T cell receptor complex formation, also interact through class I Ag.
Haagen et al. (1994)CD3T cellsFor future intravenous application we investigated whether resting T cells from peripheral blood can be stimulated to proliferate and become cytotoxic with the CD3 x CD19 bsAb alone.
Willems et al. (2005)CD3T-cellCD3 x CD28 cross-interacting bispecific antibodies improve tumor cell dependent T-cell activation.
Hara et al. (1991)CD3T-cellActivation of the autoreactive T-cell clones by cell surface binding anti-CD3 MoAb, as a specific antigen stimulator of the T-cell receptor complex, induced cell surface antigen comodulation of CD3, CD4, and WT31.
Nikolai et al. (1998)CD3T cellsWe investigated the direct effect of T-cell receptor (TCR)/CD3 complex engagement and/or stimulation of the accessory molecule CD2 on the locomotion of peripheral human T cells within three-dimensional (3-D) collagen lattices.
Testi et al. (1989)CD3T cellSoluble anti-CD3 mAb were sufficient to induce Leu 23 on the Jurkat T cell line, but a direct relationship was found between CD3 cross-linking valency and Leu 23 expression.
Garbrecht et al. (1988)CD3T cellsThe requirement for antigen and/or accessory cells could be replaced by a monoclonal antibody to the CD3 determinant of human T cells (OKT3) bound to the surface of plastic tissue culture wells.
Mølhøj et al. (2007)CD3T cellsA dimeric protein of 114kDa was obtained that showed proper bispecific binding to CD3- and CD19-positive cells and could redirect both pre-stimulated and unstimulated human T cells for lysis of human B lymphoma lines Raji, MEC-1 and Nalm-6.
Renner et al. (1996)CD3T-cellAs upregulation of the zeta chain can also be achieved using bispecific monoclonal antibodies (BI-MoAbs) with specificity for tumor antigens and CD3 and CD28, respectively, an immunotherapy with CD3/CD30 and CD28/CD30 Bi-MoAbs may overcome and should therefore, not be jeopardized by the inherent T-cell deficiency in patients with Hodgkin's disease.
Nagorsen et al. (2009)CD3T cellBlinatumomab is a single-chain bispecific antibody construct with specificity for CD19 and CD3 belonging to the class of bispecific T cell engager.
Testi et al. (1989)CD3T cellThese findings suggest that the extent of CD3/TCR cross-linking influences the persistence of elevated intracellular calcium ion concentration and PKC activation, and that this in turn determines the commitment of the T cell to activate gene transcription programs necessary for Leu 23 expression.
Renner et al. (1996)CD3T cellsAfter stimulation by combined CD3 and CD28 cross-linking, T cells from Hodgkin's disease patients upregulated zeta chain protein expression to normal values within 48 hours and achieved a cytolytic potential and levels of interleukin (IL)-2 secretion that were not different from T cells obtained from healthy controls.
Zhong et al. (2009)CD3T cellsThe ligation of TCR/CD3 on CD4 or CD8 T cells led to CD3 nanoscale co-clustering or interaction with CD4 or CD8 co-receptors forming 200-500 nm nano-domains or >500 nm micro-domains.
Poo et al. (1994)CD3T cellThis enhancement was not due to a general effect of T cell activation on transmembrane cytoskeletal proximity since CD45-phalloidin r.e.t. was not affected by CD3 ligation.
Poo et al. (1994)CD3T cellThese experiments provide direct physical evidence that ligation of the CD3 complex specifically increases the proximity of LFA-1 and microfilaments, which may be relevant to T cell mediated adherence reactions.
Haregewoin et al. (1991)CD3T cellLike alpha beta T cell clones, which recognize mycobacterial HSP, the clone requires antigen-presenting cells for antigen-induced proliferation and can also be directly activated via receptor cross-linking through CD3 or the delta chain of the gamma delta T cell receptor.
Yamagami et al. (1990)CD3T cellsFailure of T cell receptor-anti-CD3 monoclonal antibody interaction in T cells from marrow recipients to induce increases in intracellular ionized calcium.
Cheadle (2006)CD3T-cellMicromet AG and Medlmmune Inc are developing MT-103, a single-chain bispecific recombinant antibody from Micromet's BiTE (bispecific T-cell engager) product platform that binds both the CD19 antigen and the T-cell receptor (CD3), for the potential treatment of B-cell lymphoma.
Lanier et al. (1987)CD3T cellUsing detergent conditions that preserved the CD3/T cell antigen receptor complex, an approximately 90 kD disulfide-linked heterodimer, composed of approximately 45- and approximately 40- (or approximately 37-) kD subunits, was coimmunoprecipitated with the invariant 20-29-kD CD3 complex.
Tuleta et al. (2008)CD3CD3RESULTS: As a key finding, frequency of CD133, S100, GFAP, CD14 and CD3 (18.5 +/- 3.6, 14.9 +/- 1.8, 10.6 +/- 1.1, 40.2 +/- 8.3 and 5.0 +/- 0.6%, respectively) in neointima was maximal at day 7.
Mirza et al. (1993)CD3T-cellThe present work shows that cross-linking of CD3 antigen on patients' T-cell surface induces two- to threefold lower Ca2+ response than in T cells from age-matched controls.
Aniansson Zdolsek et al. (1999)CD3T-cellWe have studied prospectively the mean fluorescence intensity of the T-cell receptor complex-associated CD3, CD4 and CD8 in relation to atopic family history (AFH) and the development of atopic disease.
Huet et al. (1989)CD3T cellsHowever, several results show that CD28 and CD44 mediate different signals to the T cells: i) in contrast to CD28 mAb, CD44 mAb cannot complement the signal delivered by a soluble CD3 mAb, lectins, or PMA; ii) CD44 mAb, at the difference of CD28 mAb, cannot induce CD3+ thymocytes to proliferate in conjunction with a first signal provided via cross-linked CD3 or via CD2; iii) F(ab) fragments of H90 were efficient, whereas divalent fragments of CD29 9.3 mAb are required to produce activation signals; and iv) CD44 and CD28 mAb produce a very strong synergistic effect on T cell proliferation.
Thomassen et al. (2006)CD3T-cellsWe now have examined the interaction between CD3gamma and CD3 in human T-cells.
Thomassen et al. (2006)CD3T-cellCell-surface and intracellular expression of the introduced CD3gamma chains was determined, as was the association with CD3delta, CD3, and the T-cell receptor.
Ferrini et al. (1987)CD3T cellThese data support the concept that these clones may express, in association with CD3, the molecular product of the T cell receptor gamma genes instead of the typical alpha/beta heterodimer.
Osman et al. (1992)CD3T lymphocytesEvidence for an association between the T cell receptor/CD3 antigen complex and the CD5 antigen in human T lymphocytes.
Osman et al. (1992)CD3T cellsThese data suggested that the TcR/CD3 complex and and the CD5 antigen might be associated in T cells.
McCutcheon et al. (2004)CD3T lymphocytesFor example, T lymphocytes were identified using pan specific anti-CD3 mAb, which recognizes both alphabeta and gammadeltaT cells.
Yamagami et al. (1990)CD3T cellThis study examines recipient T cells for increases in intracellular ionized calcium concentration [( Ca2+]i) after binding the T cell receptor-CD3 complex with anti-CD3 MAb.
Seethalakshmi et al. (1996)CD3T-cellsTo rule out the possibility that the inflammation was due to con-A itself, we cross-linked some T-cells with anti-CD3 antibody before adoptive transfer (Experiment 2).
Overgård et al. (2009)CD3T-cellsThe CD3 complex is in higher vertebrates shown to be important for the activation of T-cells.
Guntermann et al. (1998)CD3T-cellIn this study, the effect of HIV-1 infection on the function of CD45-associated tyrosine phosphatase activity in the H9 T-cell line has been investigated with respect to CD3 and CD4 ligation.
Ledbetter et al. (1986)CD3T cellValency of CD3 binding and internalization of the CD3 cell-surface complex control T cell responses to second signals: distinction between effects on protein kinase C, cytoplasmic free calcium, and proliferation.
Osman et al. (1993)CD3T cellsThe close physical proximity measured between CD5 and CD3 correlates with our co-capping data and taken together the results show that the association of CD5 and the TcR/CD3 complex first detected by immunoprecipitation occurs on the surface of human T cells under physiologically relevant conditions.
Osman et al. (1993)CD3T cellAntibody-induced capping of CD5 or CD3 and double indirect immunofluorescence labeling revealed a specific co-localization of a significant fraction of CD3 and CD5 molecules on the T cell surface.
Wee et al. (1993)CD3T cellsUnlike other Tyr kinase substrates, such as the phospholipase C gamma 1-associated pp35/36 protein, whose level of Tyr phosphorylation is highest when T cells are activated by cocrosslinking CD3 with CD2, the levels of CD6 Tyr phosphorylation are highest when T cells were activated by cocrosslinking CD3 with CD4.
Hoffmann et al. (2000)CD3T lymphocytesCONCLUSION: The data indicate that competition of CD3 binding allows confirmation of the specificity of tetramer binding to the TCR, extending the usefulness of tetramers in the frequency analysis of peptide-specific T lymphocytes.
Roos et al. (1998)CD3T cellsWe developed a whole-blood lymphocyte culture system in which T cells were stimulated by a combination of CD3 and CD28 mAb.