Viewing affirmative mentions of gene expression of IL2 (H. sapiens) in thymocytes

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De la Hera et al. (1987)IL2thymocytesHere we have isolated a population of T11+3-4-6-8- human thymocytes and CD7+/T11-3-4-6-8- prothymocytes which produce and consume IL2 upon phytohemagglutinin triggering.
Reem et al. (1984)interleukin-2thymocytesRegulation of interleukin-2 synthesis in human thymocytes.
Reem et al. (1984)IL-2thymocyteAs a result of the inhibition of endogenous IL-2 synthesis, thymocyte proliferation at 120 h of culture was also inhibited.
Reem et al. (1985)interleukin 2thymocytesWe show that purified recombinant interleukin 2 (rIL-2) alone induces the expression of high- and low-affinity interleukin 2 (IL-2) receptors in vitro on human T cells and thymocytes that have not been activated previously by lectins or other inducing agents.
Reem et al. (1985)IL-2thymocytesIL-2 also upregulates the expression of high-affinity IL-2 receptors on activated thymocytes.
Reem et al. (1987)interleukin 2thymocytesThis study shows that unfractionated thymocytes can be activated to proliferate in response to activation by the CD 2 pathway, to express interleukin 2 receptors, and to synthesize interleukin 2 and interferon-gamma.
Reem et al. (1987)interleukin 2 genethymocytesLess mature, T3- thymocytes, isolated by negative selection are activated to a lesser extent than are unfractionated thymocytes; activation by the CD 2 pathway, induces proliferation, the expression of the interleukin 2 gene and interferon-gamma synthesis. 12-O-Tetradecanoyl phorbol 13-acetate in combination with the anti-CD 2 antibodies T11(2) + T11(3) increases the response of both unfractionated and T3- thymocytes.
Vives et al. (1987)interleukin 2thymocytesUnfractionated human thymocytes have a lower proliferative capacity than CD3-4-8- ones but have a similar capacity for expression of interleukin 2 receptors and production of interleukin 2.
Vives et al. (1987)interleukin 2thymocytesCD3-4-8- and unfractionated thymocytes were compared for their capacity to proliferate, to express interleukin 2 (IL-2) receptor, and to secret IL-2.
Vives et al. (1987)IL-2thymocyteSurprisingly, however, the stimulation of these populations with either phorbol ester plus IL-2 or phorbol ester plus ionophore induced a high and similar level of IL-2 receptor expression in both thymocyte populations.
Vives et al. (1987)IL-2thymocytesThese results suggest that during the maturation process, the majority of thymocytes lose their capacity to be activated by some mitogens, although they maintain their capacity to secrete IL-2 and to express the IL-2 receptor.
Roifman et al. (1986)IL-2thymocytesIncubation of mitogen-treated thymocytes with phorbol esters reconstituted IL-2 production and the proliferative response indicating that the cells were indeed activated by the mitogens.
Roifman et al. (1986)IL-2thymocyteSince an increase in [Ca2+]i is a prerequisite, and possibly a trigger, for IL-2 production, the failure of PHA, Con A, or SPA to result in thymocyte proliferation may be due to an inability of thymocytes to respond to increases in [Ca2+]i with subsequent IL-2 production.
De la Hera et al. (1986)interleukin 2thymocyteA T3 complex-bearing subpopulation was characterized within an in vivo cycling T4-8- early thymocyte compartment which contains cells constitutively expressing interleukin 2 and transferrin receptors.
Yeh et al. (1984)TCGF receptorthymocytesHuman thymocytes could be induced to express Tac antigen (TCGF receptor) on their cell surface by Concanavalin A.
Musiani et al. (1982)IL-2thymocytesThe addition of exogenous interleukin 1, a macrophage product, strongly augmented the blastic transformation of cultured thymocytes from both normal and neoplastic glands by influencing the production of interleukin 2 (IL-2) by a well-defined T-cell subset.
Musiani et al. (1982)IL-2thymocytesThe higher proliferative responses exhibited by thymocytes from thymoma were effectively sustained by a higher production of IL-2 in culture.
Blue et al. (1987)interleukin-2ThymocyteThymocyte subpopulations were activated through the T11 molecule (alternate pathway) and compared with regard to interleukin-2 (IL-2) receptor expression, changes in right-angle scatter and 3H-thymidine incorporation.
Watanabe (1986)IL2thymocytesThey were as follows; (1) mature T lymphocytes, fraction I and II, which produced IL2, responded to IL2 and had both suppressor and helper function; (2) immature thymocytes, fraction III and a part of IV, which responded to IL2 without production of IL2 and had neither suppressor nor helper function, but suppressor function was induced by Con A with IL2 from this group of thymocytes; (3) immature thymocytes, a major part of fraction IV, which had no IL2 production and no responsibility, and had neither suppressor nor helper function.
Watanabe (1986)IL2thymocytesPMA enhanced PHA induced IL2 production and IL2 responsibility of human thymocytes, and also PMA induced suppressor function from human thymocytes.
Sidell and Ramsdell (1988)IL-2thymocyteThus, RA enhancement of thymocyte responses appears to be mediated by an increase in IL-2-receptor expression on thymocyte blasts, resulting in augmented IL-2-dependent growth.
Plum and de Smedt (1987)interleukin-2thymocytesThe expression of the interleukin-2 (Il-2) receptor on the cell surface membrane of human prenatal and postnatal thymocytes has been studied by flow cytometry.
Siliciano et al. (1985)interleukin-2thymocytesIn addition, the majority of thymocytes that have not yet acquired the T3-Ti antigen/major histocompatibility complex (MHC) receptor can be activated to express interleukin-2 (IL-2) receptors through this T11 structure.
Reem and Yeh (1985)interleukin 2thymocytesModulation of the expression of interleukin 2 receptors of human thymocytes by recombinant interleukin 2.
Reem and Yeh (1985)interleukin 2thymocytesThe effect of purified recombinant interleukin 2 on the expression of the receptors for interleukin 2 by human thymocytes was examined.
Reem and Yeh (1985)interleukin 2thymocytesInterleukin 2 augmented the expression of interleukin 2 receptors and interferon-gamma synthesis by thymocytes activated with concanavalin A, and it was required to maintain the growth of thymocytes in vitro and the expression of interleukin 2 receptors.
Reem and Yeh (1985)interleukin 2thymocytesDexamethasone inhibited the expression of interleukin 2 receptors, the synthesis of interferon-gamma, and the early proliferation and protein synthesis of lectin-activated thymocytes during the first 2 days of culture.
Reem and Yeh (1985)interleukin 2thymocytesThe results of the study document that recombinant interleukin 2, like purified natural interleukin 2, is required for the expression of interleukin 2 receptors, for interferon-gamma synthesis, and for the growth of thymocytes in vitro.
Reem et al. (1986)interleukin 2thymocytesWe have recently demonstrated that human thymocytes can be induced to express interleukin 2 (IL-2) receptors and to synthesize IL-2.
Reem et al. (1986)IL-2thymocytesThe present study shows that relatively immature T6+ human thymocytes as well as the more mature T3+ thymocytes could be induced to express functional IL-2 receptors when activated with either Concanavalin A (Con A), Con A and 12-O-tetradecanoylphorbol 13-acetate (TPA) or IL-2 in combination with Con A or TPA.
Reem et al. (1986)IL-2thymocytesT6+ thymocytes proliferated in response to IL-2 and persisted in cultures for the duration of the study (18 days) and continued to express IL-2 receptors.
Reem et al. (1989)IL-2thymocytesOur observations suggest that TNF-alpha interacts with IL-2 and with another factor(s) which is induced in the course of activation by concanavalin A, since the immunosuppressant drug cyclosporin A-, which inhibits thymocyte activation, prevents the effect of TNF-alpha on thymocytes activated with concanavalin A, whereas anti-Tac, which prevents the binding of IL-2 to its receptor without affecting the production of IL-2 or the expression of IL-2-specific mRNA, inhibits proliferation only partially.
Kees et al. (1994)IL-2thymocytesPER-117 cells produce substantial levels of IL-2 and thus provide a model to study stage-specific signal transduction and transcriptional activation of the IL-2 gene in IL-1 responsive immature thymocytes.
Reem and Yeh (1985)interleukin 2thymocytesRegulation by interleukin 2 of interleukin 2 receptors and gamma-interferon synthesis by human thymocytes: augmentation of interleukin 2 receptors by interleukin 2.
Reem and Yeh (1985)IL 2thymocytesThe role of interleukin 2 (IL 2) on the expression of IL 2 receptors and on the synthesis of gamma-interferon (gamma-IFN) by human thymocytes was investigated.
Reem and Yeh (1985)IL 2thymocytesThe results indicate that thymocytes cultured in complete medium do not express receptors for IL 2, nor did IL 2 by itself induce the expression of IL 2 receptors.
Reem and Yeh (1985)IL 2thymocyteCon A induced the expression of IL 2 receptors by a moderate number of the thymocyte population and induced the synthesis of low amounts of gamma-IFN.
Reem and Yeh (1985)IL 2thymocytesThe expression of IL 2 receptors could be detected within 24 hr and preceded the induction of proliferation; it was therefore probably not due to the clonal expansion of a population of receptor-bearing thymocytes.
Reem and Yeh (1985)IL 2thymocytesTherefore, when IL 2 was prevented from binding to the receptors, and IL 2 synthesis was inhibited, the number of thymocytes expressing IL 2 receptors was sharply reduced and gamma-IFN synthesis was markedly inhibited.
Maguer et al. (1993)IL-2thymocytesWe further verified that the proliferation of human thymocytes is consecutive to the expression of IL-2 receptors and the synthesis of IL-2.
Wustrow (1991)IL-2thymocytesInterleukin 2 (IL-2) is predominantly produced by T-helper cells (TH1) having the phenotype CD4+, and by subpopulations of thymocytes after antigenic or mitogenic stimulation.
Cosmi et al. (2003)interleukin 2thymocytesThe suppressive activity of CD8+CD25+ thymocytes was abrogated by a mixture of anti-CTLA-4 and anti-TGF-beta1 antibodies and it was mediated by their ability to inhibit the expression of the interleukin 2 receptor alpha chain on target T cells.
Bodey et al. (1996)interleukin-2thymocytesThe results indicated that thymic humoral factors contribute to a select, not fully understood differentiation pathway of thymocytes: a) more mature immunophenotype (IP) characterized by CD3 expression; b) de novo synthesis of interleukin-2 receptor (IL-2R); and; c) differentiation of the CD8+ subpopulation, identifying regulatory cells within the two major CD8+ and CD4+ subsets.
Kasahara et al. (1987)IL 2thymocyteNot only IL 1 alpha activity in the thymocyte costimulator assay, but also IL 1-dependent IL 2 production by a human leukemic cell line, HSB.2 subclone, were blocked by these polyclonal or monoclonal Ab.
Fox et al. (1985)interleukin 2thymocytesActivation of human thymocytes via the 50KD T11 sheep erythrocyte binding protein induces the expression of interleukin 2 receptors on both T3+ and T3- populations.
Yeh et al. (1984)TCGFthymocytesAnti-Tac antibody (10(-3)) inhibited the expression of TCGF receptors and late proliferation of thymocytes.
Blue et al. (1986)IL-2thymocytesThe thymocytes (greater than 35%) that expressed the transferrin and IL-2 receptors demonstrated nuclear activation as measured by log 90 degrees light scatter analysis.
Simi? et al. (1986)IL2thymocytesOn the other side, HCS also enhances the lectin-induced production of IL2 by thymocytes.
Carding et al. (1989)IL-2thymocytesIn 13-day fetal thymocytes a population of cells constitutively produces low levels of interleukin 2 (IL-2) and interleukin 4 (IL-4) mRNAs.
Carding et al. (1989)lymphokinethymocytesOur findings indicate developmental control of lymphokine and lymphokine receptor gene expression in fetal thymocytes during ontogeny.
Lewis et al. (1991)IL-2 mRNAthymocytesIL-4, IFN-gamma, and IL-2 mRNA expression by neonatal CD4+CD8- thymocytes was similar to that found in circulating neonatal CD4+ T cells.
Wu et al. (1989)IL 2thymocytesBoth factors had similar activities: inhibition of IL 2 production by human peripheral blood mononuclear cells, inhibition of IL 2 responsiveness of human lymphoblasts and mouse thymocytes, and inhibition of proliferation of Con A-stimulated mouse splenocytes.
de la Hera et al. (1985)IL-2thymocytesHuman thymocytes bearing T3 but neither T4 nor T8 antigens (T3+4-8- cells) were obtained after negative selection of thymocytes, either fresh or cultured in medium containing recombinant interleukin 2 (IL-2), by treatment with Na1/34, OKT4A and B9.4 monoclonal antibodies (which recognize T6, T4, and T8 antigens, respectively) and complement.
Raulet (1985)interleukin-2thymocytesExpression and function of interleukin-2 receptors on immature thymocytes.
Reem et al. (1986)IL-2thymocytesIn thymocytes activated in vitro the expression of IL-2 receptors, determined by dual colour cytofluorometry with the PE-conjugated monoclonal anti-human IL-2 receptor antibody (PE anti-IL-2 R), was detected by the second day of induction in both immature T6+ and mature T3+ thymocytes.
Selvaraj et al. (1987)IL-2thymocyteCertain combinations of monoclonal antibodies to CD2 epitopes trigger proliferation of peripheral blood T lymphocytes, cytotoxic effector function and expression of IL-2 receptors by thymocytes, resulting in thymocyte proliferation in the presence of exogenous IL-2 (ref. 11).
Katagiri et al. (1987)IL 2thymocytesThe unusual functional properties of the lpr cells, such as high A23187 dose requirement for maximal proliferation, low percentage of IL 2 receptor-expressing cells, and low levels of IL 2 secretion, suggested that these cells are arrested at a stage of development similar to that of 16-day fetal thymocytes and before adult L3T4-/Lyt-2- thymocytes.
Reem et al. (1989)IL-2thymocytesWe have provided evidence that tumor necrosis factor alpha (TNF-alpha) enhances the proliferation and the state of activation of human thymocytes cultured with concanavalin A or interleukin 2 (IL-2), as evidenced by an increase in the expression of the c-myc gene and the gene of the IL-2 receptor (alpha-chain, Tac antigen) and by the expression of Tac antigen on the cell surface.
Toribio et al. (1983)IL 2thymocytesSupernatants containing interleukin 2 (IL 2) induce strong proliferation and expression of natural killer (NK)-like activity in human thymocytes.
Ranelletti et al. (1986)IL-2thymocytesFurthermore, in the presence of exogenous IL-1, Fr1 thymocytes showed a greater capacity for producing IL-2.
Reem and Yeh (1985)IL 2ThymocytesThymocytes activated with TPA and Con A were more resistant to the inhibitory effects of Dex on the expression of IL 2 receptors than thymocytes activated with Con A alone.
Reem and Yeh (1985)IL 2thymocytesConversely, inhibition of IL 2 synthesis with dexamethasone (Dex) by thymocytes activated with Con A, or inhibition of the function of IL 2 receptors by anti-Tac, resulted in a decrease in the number of IL 2 receptor-bearing thymocytes activated with Con A, or inhibition of the function of IL 2 receptors by anti-Tac, resulted in a decrease in the number of IL 2 receptor-bearing thymocytes and of gamma-IFN synthesis.
Dudich et al. (1995)IL2thymocyteSoluble class I antigens were shown to costimulate IL2 production by thymocytes in response to submitogenic doses of exogenous IL2 and to increase PHA-induced thymocyte proliferation.
Simi? et al. (1986)IL2thymocytesThus, HCS enhances the lectin-induced expression of IL2-receptors as evidenced by both increased responsiveness to IL2 and increased capacity to absorb IL2 of HCS-treated thymocytes.
Carding et al. (1989)lymphokinethymocytesDevelopmental control of lymphokine gene expression in fetal thymocytes during T-cell ontogeny.
Carding et al. (1989)lymphokinethymocytesWe have used the technique of in situ hybridization to investigate the expression of lymphokine genes by immature thymocytes during intrathymic development.
Carding et al. (1989)lymphokinethymocytesA second phase of lymphokine gene expression occurs in the majority of 15-day thymocytes, and a population of cells constitutively produces both IL-2 and IL-4 mRNAs.
Carding et al. (1989)IL-2thymocytesBy contrast, the population of IL-2 receptor mRNA-producing thymocytes increases progressively up to 15 days of gestation, and expression thereafter decreases up to birth.
Musiani et al. (1985)IL-2thymocytesThis implies that the endogenous IL-2 production by thymocytes is inadequate to fully support the intrinsic proliferative capacity of these cells.
Ueno et al. (1989)Interleukin-2thymocytesInterleukin-2 receptors (IL-2R) are expressed on minor populations of immature and mature human thymocytes.
Ueno et al. (1989)IL-2thymocytesThese data indicate that mature thymocytes can express a functional high affinity receptor for IL-2 and suggest that immature thymocytes may not possess a (functional) p75 chain of the IL-2R.
Piantelli et al. (1986)IL 2thymocytesHowever, these thymocytes are incapable of producing the adequate amount of IL 2 required to fully saturate their intrinsic proliferative capability.
Piantelli et al. (1986)IL 2thymocytesIt is noteworthy that a large proportion of these T3- and T6-negative thymocytes express IL 2 receptors and class II MHC antigens without in vitro activation.
Fox et al. (1985)IL 2thymocytesThe triggering of T11 by monoclonal antibodies anti-T112 and anti-T113, directed at two unique epitopes on the molecule, induced IL 2 receptor expression on both T3+ and T3- thymocytes but did not induce IL 2 production.
Blue et al. (1986)IL2thymocytesThe third stage of maturation (day 5-7) represented thymocytes with reduced levels of activation as measured by forward and right angle light scatter analysis and declining IL2 and transferrin receptor expression.
Bárcena et al. (1991)IL-2thymocytesIn contrast to peripheral lymphocytes, IL-4 does not down-regulate the expression of the IL-2 receptor light chain on thymocytes.
Chang (1995)IL-2thymocyteHowever, at doses of amiprilose HCl previously found to stimulate thymocyte proliferation (1-10 micrograms/ml), increased levels of culture supernatant IL-2 were observed.
Yagi et al. (1987)IL 2thymocytesThese IL 2-activated thymocytes expressed higher levels of both Thy 1, Lyt 2 and lymphokine-activated cell-associated (LAA) antigens than unstimulated thymocytes, as indicated by a flow cytometric analysis.