Viewing affirmative mentions of transcription of IL2 (H. sapiens) in lymphocytes

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Yamamoto et al. (1985)IL2lymphocytesInduction of interleukin 2 (IL2) mRNA synthesis in human tonsillar lymphocytes was studied by quantifying the relative levels of IL2 mRNA in the lymphocytes stimulated under various conditions by the dot hybridization method.
Ohmura and Onoue (1990)IL-2 cDNAlymphocytesFurther study demonstrated that the rate of degradation of 32P-labeled IL-2 mRNA, which was prepared by cell-free transcription of IL-2 cDNA, in the polysomal fraction obtained from PDB-stimulated lymphocytes was decreased compared with that obtained from unstimulated lymphocytes.
Oh et al. (1993)IL-2lymphocyteConcomitant with the inhibition of lymphocyte activation and interleukin 2 (IL-2) production, transcription of the IL-2 message is also reduced in a time-dependent manner.
Vitolo et al. (1992)IL2lymphocytesIn contrast, tumor-infiltrating lymphocytes (TIL) in the stroma of ovarian carcinomas or most ductal breast tumors only rarely expressed mRNA for TNF alpha, IL2 or IFN gamma.
Tanaka and Tsujimura (2002)IL-2lymphocytesCyclosporin is a non-cytotoxic immunomodulating drug which inhibits NF-AT-dependent IL-2 transcription in lymphocytes.
Coventry et al. (1996)Il-2lymphocytesLack of IL-2 cytokine expression despite Il-2 messenger RNA transcription in tumor-infiltrating lymphocytes in primary human breast carcinoma: selective expression of early activation markers.
Faulds et al. (1993)interleukin-2lymphocytesCyclosporin is a lipophilic cyclic polypeptide which produces calcium-dependent, specific, reversible inhibition of transcription of interleukin-2 and several other cytokines, most notably in T helper lymphocytes.
Lopez et al. (1998)interleukin-2 mRNAlymphocytesRepression of interleukin-2 mRNA translation in primary human breast carcinoma tumor-infiltrating lymphocytes.
Ye et al. (2001)IL-2 mRNAPBLsRESULTS: The morphology of IL-2-transduced 3AO and IL-2R-transduced PBLs remained unchanged. 3AO cells could be transfected with the IL-2 gene and expressed IL-2 mRNA, and PBLs could be transfected with the IL-2R gene and expressed IL-2R mRNA.
Lecron et al. (1987)IL2lymphocytes[Production by B+ null lymphocytes of an activity increasing the proliferation of T CD4+ lymphocytes in the presence of phytohemagglutinin and IL2].
Riesbeck and Forsgren (1995)interleukin-2lymphocytesBecause ciprofloxacin has been reported to strongly enhance interleukin-2 production, we considered it important to study the effect of CP-115,953 on interleukin-2 and gamma interferon (IFN-gamma) mRNA and protein expression in mitogen-stimulated human peripheral blood lymphocytes.
Filgueira et al. (1993)IL-2 geneTILIn contrast, rhIL-2 could not induce IL-2 gene transcription in five of eight TIL cultures.
Tanaka et al. (1995)IL-2lymphocyteOn the other hand, we have reported that increased mRNA expression and protein product of IL-2 and IFN-gamma were evident in the mixed lymphocyte culture of the cases who developed severe lethal transplantation-related complications.
Chisholm (1991)lymphokinelymphocyteNumerous studies over the last decade have shown quite clearly that, in vitro, CS inhibits lymphocyte activation at a relatively early stage by preventing the production and release of lymphokines, probably by inhibition of lymphokine gene transcription.
Goebel et al. (2002)IL-2lymphocyteIn the next week, re-exposure to the conditioned stimulus (drink), but now paired with placebo capsules, induced a suppression of immune functions as analyzed by the IL-2 and IFN-gamma mRNA expression, intracellular production, and in vitro release of IL-2 and IFN-gamma, as well as lymphocyte proliferation.
Reed et al. (1988)IL-2lymphocytesUsed at concentrations that inhibited proliferation of human peripheral blood lymphocytes by approximately 90%, CsA suppressed, to different extents, the phytohemagglutinin-stimulated expression of various genes, with levels of mRNAs for IL-2 being inhibited by approximately 100%, c-myc and N-ras by approximately 80%, and c-fos and IL-2 receptors by approximately 50%.
Pack et al. (2002)interleukin-2HPBLFurthermore, LA had different effects on the secretion of interleukin-2 (IL-2) and steady-state levels of IL-2 mRNA in mitogen-stimulated HPBL depending on the mitogens used.
Nagel et al. (1988)IL2lymphocytesThe results suggest that decreased expression of both IL2 and IL2R mRNA contributes to the low synthesis of IL2 and membrane IL2R, respectively, and is partially responsible for the diminished proliferative activity observed in lymphocytes from the elderly.
Flescher et al. (1994)IL-2lymphocytesProducts of polyamine oxidase activity, at micromolar levels and during a period of 2 to 3 days, down-regulate IL-2 mRNA levels and activity in human lymphocytes.
Mullin et al. (1992)IL-2lymphocytesLevels of IL-2 mRNA in peripheral blood lymphocytes were low and not significantly different in all groups of subjects.
Shoda et al. (1998)IL-2lymphocytesTo examine the kinetics of IL-2 mRNA expression, northern elephant seal lymphocytes were stimulated with the mitogen concanavalin A (Con A), and RNA was collected at several time points thereafter.
Skvara et al. (2008)IL2lymphocyteEx vivo stimulation of lymphocytes from subjects exposed to single doses of AEB071 resulted in a dose-dependent inhibition of both lymphocyte proliferation and IL2 mRNA expression.
Croll et al. (1987)interleukin-2lymphocytesIn addition, using different protocols of stimulation of lymphocytes it was shown that both interleukin-2 and interferon-gamma mRNA steady state levels varied in concert.
Wagner et al. (1998)IL2lymphocyteWhereas the presence of a lymphocyte infiltrate in tissue samples was associated with a TH1 cytokine mRNA profile (TNF-alpha, INF-gamma, IL12p35, IL12p40, IL2Rbeta, and IL2Rgamma), clinically and histologically regressive samples exhibited additionally increased transcript levels for GM-CSF, IL2, and IL15. mRNAs of TH2 cytokines IL4 and IL5 were detected only in a minor portion of progressive melanoma samples and regressive melanoma lesions.
Wu et al. (1986)IL-2lymphocytesUsing a cDNA probe to IL-2, the effect of age on the expression of IL-2 mRNA was studied on concanavalin A-stimulated lymphocytes.
Wu et al. (1986)IL-2lymphocyteTherefore, the age-related decline in the ability of lymphocyte population to produce IL-2 arises from a decrease in the ability of the lymphocytes to genetically express IL-2 mRNA.
Chou et al. (1995)IL-2 mRNAlymphocytesBoth IL-2 mRNA and IL-4 mRNA were detected in various monkey T cells as well as human peripheral blood lymphocytes infected with wild-type H. saimiri.
Nachbaur et al. (1997)IL-2lymphocyteReduced interleukin-2 (IL-2) protein levels in culture supernatants of IVIG-supplemented mixed lymphocyte reactions (MLR) but unchanged IL-2 mRNA levels strongly argue in favour of a post-transcriptional interference of IVIG with cytokines and/or cytokine production.
O'Beirne et al. (2010)interleukin 2lymphocytesGPC-3: glypican-3; HCC: hepatocellular carcinoma; PBMC: peripheral blood mononuclear cells; DC: dendritic cells; LPS: Lipopolysaccharide; TIL: tumour infiltrating lymphocytes; IL-2: interleukin 2; TAA: tumour-associated antigens; CTL: cytotoxic T lymphocyte; TAP: transporters associated with antigen processing; IL-4: interleukin-4; eGFP: enhanced green fluorescent protein.
Simonsson et al. (1991)lymphokinelymphocytesThe kinetics of lymphokine-specific DNA transcription during in vitro immunization of human peripheral blood lymphocytes and splenocytes were studied using the polymerase chain reaction.
Belldegrun et al. (1989)lymphokinelymphocytesTumor infiltrating lymphocytes (TIL) isolated from 12 patients with metastatic malignant melanoma, renal cell carcinoma, or breast adenocarcinoma were expanded in rIL-2 for 22 to 45 days (median 33 days) and analyzed for lymphokine mRNA expression and patterns of TCR gene rearrangement.
Uemura (1993)IL-2lymphocyteMeDSG did not have effects on the production of IL-1, IL-2 and the expression of IL-2 receptor on stimulated lymphocyte by mitogen.
Kotzsch et al. (1982)lymphokinelymphocyteStudies on lymphokine activity in concanavalin A-stimulated lymphocyte supernatants using the macrophage-electrophoretic-mobility-test (MEM-test).
Koike (1989)interleukin-2lymphocytesWith special reference to the production of interleukin-2 and the expression of interleukin-2 receptors in the patients' peripheral blood lymphocytes.
Roussel et al. (1995)lymphokineTILTIL expressed detectable lymphokine mRNA but seemed poorly activated with respect to the total number of lymphokine genes and the amount of mRNA compared with alpha-CD3-activated healthy PBL.
Shoker et al. (1997)interleukin-2lymphocytesThe immune parameter studies were proliferation and interleukin-2 production by peripheral blood lymphocytes stimulated with anti-CD3 antibody, phytohemagglutinin and alloantigens; proliferation, interleukin-2 production and mRNA expression of interleukin-4 and interferon gamma by allogeneic CD4+ T-cell clones stimulated with alloantigens.
Taniguchi et al. (1994)interleukin-2lymphocytesThe assay was successfully used to quantitate mRNA levels of the T cell derived cytokines interleukin-2, interleukin-4 and interferon-gamma in resting and stimulated human lymphocytes.
Hallet et al. (1992)lymphokinelymphocyteExcept, perhaps for IL-6, the pattern of lymphokine transcription did not permit us to distinguish between different lymphocyte subsets.
Kierszenbaum et al. (2002)IL-2lymphocytesSupporting this view were the substantially diminished levels of IL-2, CD25, CD122, and CD132 mRNA found in AGC10-containing cultures of PHA-activated lymphocytes.
Andrianifahanana et al. (2006)IL-2lymphocytesMoreover, transcript for interleukin 2 (IL-2), a known marker of activated T helper 1 (TH1) lymphocytes, exhibited an expression profile similar to IFNgamma, suggesting a role of these immune effector cells as a potential source of IFNgamma in PA.
Yang et al. (2002)IL-2CBLIn the phytohemagglutinin-stimulated CBL culture system, when the IGF-IR antisense oligonucleotide existed, the mRNA levels of IFN gamma and IL-2 decreased 30-50% and IL-4 decreased 20-30%.
Lendeckel et al. (1999)IL-2lymphocyteThis review outlines evidence for APN contributing to the regulation and realisation of lymphocyte growth and function by modulating the mRNA expression of IL-2, IL-1 receptor antagonist, and TGF-beta1 and increasing the activity of MAP kinase p42/Erk2.
Conlon et al. (1995)lymphokinelymphocyteNorthern analysis revealed a parallel pattern of lymphokine mRNA expression in the four lymphocyte subsets.
Baan et al. (1997)IL-2GILDuring cardiac rejection we studied the kinetics of IL-2 and IL-4 mRNA and subsequent protein production by in vivo primed graft-infiltrating lymphocytes (GIL), using semiquantitative RT-PCR and ELISA.
Castigli et al. (1995)IL-2lymphocytesImportantly, the levels of mRNA encoding c-myc, IL-2R alpha, IL-2 and IFN-gamma were markedly decreased in patient lymphocytes stimulated with PMA+ionomycin as compared to control lymphocytes.
Sakatsume et al. (2001)interleukin-2lymphocyteMoreover, these urine cells expressed mRNA of the T helper lymphocyte 1 cytokines, interleukin-2, and/or interferon-gamma.
Vitolo et al. (1992)IL2TILIn situ hybridization for cytokine mRNA performed with tumor tissues, and LN of patients with HNC showed that TIL as well as LNL localized in the immediate proximity of the tumor were activated, as evidenced by the expression of mRNA for IL2, IFN-gamma, IL-1 beta, TNF-alpha, and both alpha- and beta-chains of the IL2 receptor.
Baan et al. (1997)IL-2lymphocytesKinetics of IL-2 and IL-4 mRNA and protein production by graft-infiltrating lymphocytes responsible for rejection after clinical heart transplantation.
Cruikshank et al. (1991)lymphokinelymphocytesWe investigated whether a previously reported human lymphokine, lymphocyte chemoattractant factor (LCF), which was described for its effects on human CD4+ lymphocytes and monocytes, could associate directly with CD4 and induce the generation of second messengers.
Fukuda et al. (1995)IL2lymphocyteExpression of the mRNA of the cytokines IL1-beta, IL2, IL4, IL5, IL6, TNF-alpha, and IFN-gamma, the lymphocyte markers CD4 and CD8, and the HLA class I molecule, beta 2-microglobulin (B2MG) in the liver tissue of 20 CH(C) cases and 9 CH(B) patients was investigated by the reverse transcription polymerase chain reaction (RT-PCR) method.
Pomer (1994)lymphokinelymphocyteSeveral new drugs have been found to be effective immunosuppressive agents: FK 506, a macrolid antibiotic inhibiting lymphokine gene transcription, lymphokine production and secretion, rapamycin, which blocks effects of lymphokine-induced signal transduction, and RS 61443, brequinar and mizoribine, which inhibit DNA/RNA synthesis and lymphocyte proliferation.
Zeitz et al. (1988)IL-2LPLThis finding was confirmed at the molecular level as IL-2 mRNA was not detected in unstimulated LPL but was found in concanavalin A-stimulated LPL.
Müller-Quernheim et al. (1989)IL-2lymphocytesIn peripheral blood lymphocytes, maximal IL-2R mRNA accumulation is found between 6 and 24 h, and maximal accumulation of IL-2 mRNA is found between 24 and 48 h.
Baan et al. (2007)IL-2lymphocyteMATERIALS: To explore the actions of IL-21 with other gammac-dependent cytokines in alloreactivity, mRNA expression of IL-21, IL-21R alpha-chain, and IL-2 proliferation and cytotoxicity was measured after stimulation in mixed lymphocyte reactions.
Baan et al. (1996)IL-2GILOnly stimulation of GIL with donor-specific antigen resulted in early detectable IL-2 and IL-4 mRNA and protein levels.
Baan et al. (1996)IL-2GILMaximal relative IL-2 mRNA levels were significantly higher than maximal relative IL-4 mRNA levels (100-fold) in both GIL and PBMC after donor-specific stimulation.
Baan et al. (1996)IL-2GILThese results suggest that, after stimulation donor-specific "primed" GIL, and not "naive" PBMC, rapidly produce abundant levels of IL-2 (Th1) and IL-4 (Th2) at both the transcriptional and protein level.
Barnes et al. (1992)IL-2lymphocytesIn contrast, LAM did not induce transcription of mRNA for cytokines produced predominantly by lymphocytes, such as lymphotoxin, IFN-gamma, IL-2, IL-3, or IL-4.
Gorczynski et al. (1996)IL-2PBLRESULTS: PBL transcription of IL-2, IL-6, and interferon-gamma was significantly increased in transplant recipients with rejection compared with that in recipients without rejection or healthy individuals who did not undergo transplantation (p < 0.05).
Zuo et al. (2001)IL-2lymphocyteAntithrombin III inhibits lymphocyte proliferation, immunoglobulin production and mRNA expression of lymphocyte growth factors (IL-2, gamma-IFN and IL-4) in vitro.
Richards et al. (1992)interleukin-2lymphocytesInhibition of the serine/threonine protein phosphatases PP1 and PP2A in lymphocytes: effect on mRNA levels for interleukin-2, IL-2R alpha, krox-24, p53, hsc70 and cyclophilin.
Heussler et al. (1992)IL-2lymphocytesConstitutive IL-2 mRNA expression in lymphocytes, infected with the intracellular parasite Theileria parva.
Ryffel et al. (1992)interleukin-2lymphocyteCiclophilin is likely involved in the blockade of lymphocyte activation-induced gene transcription of various growth factors, especially interleukin-2.
Paaventhan et al. (2003)IL-2lymphocyteFve, a major fruiting body protein from Flammulina velutipes, a mushroom possessing immunomodulatory activity, stimulates lymphocyte mitogenesis, suppresses systemic anaphylaxis reactions and edema, enhances transcription of IL-2, IFN-gamma and TNF-alpha, and hemagglutinates red blood cells.
Kim et al. (1995)IL-2TILIL-2 and IFN-gamma mRNAs were expressed in TIL, while IL-10 mRNA were strongly expressed in BCC lines.
Li et al. (2003)IL-2lymphocytesPATIENTS AND METHODS: Using in situ hybridization (ISH), we determined the mRNA expression in lymphocytes and tumor cells for IL-2, INF-gamma, IL-12 (p40), IL-18, IL-4, IL-10, TGF-beta1, IL-1, IL-3, IL-8, granulocyte-macrophage colony-stimulating factor (GM-CSF), TNF-alpha, and TGF-alpha in five fresh pleural effusion samples and 18 tumor tissue samples of patients with NSCLC.
James (1996)interleukin 2lymphocyteIt blocks interleukin 2 transcription thereby inhibiting the CD4 Th-1 lymphocyte response to foreign antigens.
Chen et al. (2005)IL-2lymphocytesMETHODS: Using in situ hybridization (ISH), the expressions of IL-2, INF-gamma, IL-12 (p40), IL-18, IL-4, IL-10, TGF-beta1, IL-1, IL-3, IL-8, GM-CSF, TNF-alpha and TGF-alpha mRNAs in lymphocytes and tumor cells from five fresh pleural effusion samples and 18 tumor tissue samples of NSCLC patients were detected by in situ hybridization with digoxin end-labeled oligonucleotide probe, using 5 tuberculous pleurisy patients as control.
McKean et al. (1995)lymphokinelymphocytesPrevious studies have demonstrated that IL-1 receptor (IL-1R)- and TCR-initiated signals can interact synergistically to increase the rate of transcription of several lymphokine and lymphokine receptor genes during the competence phase of the activation program in T helper lymphocytes.
Watson et al. (1994)IL-2ITLThe presence of IL-2, IFN-gamma, and TNF alpha and the absence of IL-4 mRNA in samples of ITL indicate a pattern of cytokine production that most closely resembles that of the TH1 helper T-cell subset.
Elsässer-Beile et al. (1996)IL-2PBLThese pure CD3-positive PBL (CD3+PBL) and TIL (CD3+TIL) were cultured with pokeweed mitogen and the levels of the cytokines interleukin-1alpha (IL-1 alpha), IL-1 beta, IL-2, interferon gamma (IFNgamma), and tumor necrosis factor alpha (TNFalpha) measured in the 4-day post-inductional cell culture supernatants.
Malec et al. (2004)IL-2lymphocyteDESIGN AND METHODS: We used real-time quantitative polymerase chain reaction (RQ-PCR) to investigate mRNA expression for interleukin (IL)-1alpha, IL-1beta, IL-2, IL-4, IL-5, IL-8, IL-12p35, IL-12p40, IL-13, IL-15, interferon (IFN)-gamma and tumor necrosis factor (TNF-alpha) in lymph node tissue from 15 patients with classic Hodgkin's lymphoma (c-HL) and one with lymphocyte predominance (LP) HL.
Lafont et al. (1997)IL2 genelymphocytesHere we show that jacalin triggers IL2 gene transcription only in CD4(+) lymphocytes.