Viewing affirmative mentions of gene expression of Il4 (R. norvegicus) in T cells

Full-text article links are indicated by after the article reference.

Document Target Regulator Anatomy Sentence
Gillespie et al. (1995)IL-4T lymphocytesCircumstantial evidence implicates the Th2 subset of CD4+ T lymphocytes, which produces IL-4.
Shi et al. (2004)interleukin-4T lymphocytesBy observing the production of interferon-gamma (IFN-gamma) and interleukin-4 (IL-4) released by peripheral T lymphocytes in asthmatic rat models, this study was designed to clarify the changes of T lymphocytes during the course of airway inflammation.
Shi et al. (2004)IL-4T lymphocytesThe influence of dexamethasone pre-treatment on the production of IFN-gamma and IL-4 by T lymphocytes was also investigated in the study.
Savignac et al. (2001)IL-4T cellGold is a T cell polyclonal activator in BN and LEW rats but favors IL-4 expression only in autoimmune prone BN rats.
Savignac et al. (2001)IL-4T cellsGold salts act on early steps of transduction in T cells from BN and LEW rats since they trigger tyrosine phosphorylation of numerous proteins including p56(lck) and a calcium signal which results in IL-4 and IFN-gamma expression by BN and LEW T cells.
Askenase (2001)IL-4T cellsThe essential CS-inducing NK T cells activate the B-1 cells by producing IL-4 rapidly (1 hour) after immunization, and gammadelta-T cells assist the local inflammatory function of the recruited CS-effector alphabeta-T cells.
Prigent et al. (1995)IL-4T cellsThe early effect on IL-4 production observed on BN rat spleen cells and T cells may explain that the autoreactive anti-class II T cells that are found in HgCl2-injected BN rats have a Th2 phenotype.
Huang et al. (2003)IL-4T-cellEarly administration was more effective in preventing rejection and demonstrated a more marked effect on IL-4 expression and alloantibody deposition than on graft T-cell infiltrate and expression of other cytokines.
Noble et al. (1993)IL-4T cellsThese results indicate that: (1) IL-4 is essential for the generation of Th2-like cells; (2) IFN-gamma inhibits IL-4 production by mixed spleen cells and suppresses generation of IL-4 responsive T cells; (3) in mixed spleen cell cultures mitogenic stimulation favours differentiation of naive rat T cells into effector cells expressing a Th1, and not Th2, cytokine profile.
Badou et al. (2001)IL-4T cellsThe fact that HgCl(2) induces in vitro mRNA IL-4 gene expression in normal BN T cells but not in LEW T cells is probably crucial to susceptibility to the development of autoimmunity in the sense that it may condition the development of autoreactive T cells into pathogenic T(H)2 cells; a test for this condition is therefore also included.
Weiss and Brown (2001)IL-4 geneT cellsIn this article we review the current knowledge regarding the cell-type specific regulation of IL-4 gene expression in mast cells and compare this to what has been defined in T cells.
Pang et al. (2004)IL-4T lymphocytesThe expression pattern of IFN-gamma and IL-4 were observed at mRNA level in T lymphocytes isolated from spleen by RT-PCR.
Pascual et al. (1997)IL-4T cellsCoimmunization with CT rescued SHR CD4+ T cells from suppression and supported DT- or B subunit of CT-specific proliferative responses, and these cells produced more interleukin-4 (IL-4) than IFN-gamma, and anti-IFN-gamma antibody treatment enhanced IL-4 production.
Kasaian et al. (1996)IL-4T cellsThe assay of in vitro IL-4 production has often been used to compare the allergen responses of T cells isolated from atopic and non-atopic subjects.
Kasaian et al. (1996)IL-4T cellAmong unfractionated peripheral blood mononuclear cells (PBMC), the major producers of detectable IL-4 in primary in vitro cultures were found to be basophils based on: (i) an allergen dose-response corresponding closely to that required for basophil histamine release and lower than that required for T cell activation; (ii) a rapid time course for IL-4 production (detectable at 3 h), inconsistent with the typical activation requirements of fresh T cells; (iii) the production of comparable levels of IL-4 in cultures stimulated with allergen or anti-IgE; and (iv) the complete loss of detectable IL-4 production following specific depletion of basophils from PBMC.
Park et al. (2006)interleukin-4T cellsInhibition of interleukin-4 production in activated T cells via down-regulation of NF-AT DNA binding activity by apigenin, a flavonoid present in dietary plants.
Park et al. (2006)IL-4T cellsHowever, the mechanism by which apigenin suppresses IL-4 production especially in T cells remains unclear.
Park et al. (2006)IL-4T cellsIn this study, the effect of apigenin and its underlying mechanism on IL-4 production were investigated in activated T cells.
Park et al. (2006)IL-4T cellsIn addition, apigenin inhibited NF-AT DNA binding activities, indicating that apigenin may inhibit IL-4 production in T cells via down-regulation of NF-AT DNA binding activity.
Badou et al. (1997)interleukin-4 geneT cellsHgCl2-induced interleukin-4 gene expression in T cells involves a protein kinase C-dependent calcium influx through L-type calcium channels.
Badou et al. (1997)IL-4T cellBy using two murine T cell hybridomas that express IL-4 mRNA upon stimulation with HgCl2, we demonstrate that: 1) HgCl2 acts at the transcriptional level without requiring de novo protein synthesis; 2) HgCl2 induces a protein kinase C-dependent Ca2+ influx through L-type calcium channels; 3) calcium/calcineurin-dependent pathway and protein kinase C activation are both implicated in HgCl2-induced IL-4 gene expression; and 4) HgCl2 can activate directly protein kinase C, which might be one of the main intracellular target for HgCl2.
Brown and Hural (1997)IL-4T-cellRecently, progress has been made in defining the T-cell- and Fc epsilon R1-receptor-mediated signals that stimulate IL-4 gene expression.
Veenstra et al. (2008)IL-4T cellsHigh levels of intracellular IL-4 are expressed in circulating apoptotic T cells in patients with tuberculosis and in community controls.
Veenstra et al. (2008)interleukin-4T lymphocyteT lymphocyte intracellular interleukin-4 production in response to CD3 stimulation was determined by flow cytometry in 21 TB patients and 14 community controls.
Veenstra et al. (2008)IL-4T cellsA low percentage of CD4 T cells in both patients and controls expressed high levels of interleukin-4 (IL-4(high)).
Veenstra et al. (2008)IL-4T cellsA larger subset of both CD4 and CD8 T cells of all subjects expressed low levels of intracellular IL-4 (IL-4(low)).
Veenstra et al. (2008)IL-4T cellsIL-4 levels may therefore not necessarily indicate a skewed Th cell phenotype, as our data suggest that IL-4 production by CD4 and CD8 T cells can occur constitutively in healthy controls with latent TB infection and in TB patients.
Verhoef et al. (1999)interleukin-4T cellLymphocyte stimulation by CD3-CD28 enables detection of low T cell interferon-gamma and interleukin-4 production in rheumatoid arthritis.
Verhoef et al. (1999)IL-4T cellsIn the present study we examined whether stimulation of peripheral blood T cells in the context of mononuclear cells (PB MNC) by CD3-CD28 is a reliable method for assessing IFN-gamma and IL-4 production and is representative for the spontaneous production of these cytokines.
Verhoef et al. (1999)IL-4T-cellThe results demonstrate that a pecific T-cell stimulation by CD3-CD28 is a reliable way to enhance IFN-gamma and IL-4 production above the detection limit and so measure the T1/T2 cell balance in RA.
Prigent et al. (1995)IL-4T cellsUnfractionated BN rat splenocytes and purified T cells exposed to HgCl2 expressed high levels of IL-4 mRNA.
King et al. (2000)IL-4T cellThe T cell-derived cytokines IL-4, IL-13, and IFN-gamma were detected only during the rejection phase in allogeneic recipients.
Zipris et al. (1996)IL-4T cellsIL-2 and IL-4 mRNAs were minimal or undetectable in infiltrated islets but present in activated peripheral T cells.
Liu et al. (2007)IL-4T cellsT cells were purified from blood of each rat and the expression of PI-3K was observed by immunocytochemical fluorescence staining, the semiquantitative fluorescence intensity was measured by HPIAS-2000 analytic software, and the expression of IL-4 in supernatants was detected by ELISA.
Liu et al. (2007)IL-4 proteinT lymphocytesThe expression levels of IL-4 protein in supernatants of asthmatic T lymphocytes were significantly higher than those in the normal controls (P<0.05).
Liu et al. (2007)IL-4 proteinT lymphocytesThere was a significant positive correlation between the expression of PI-3K in T lymphocytes and the IL-4 protein expression in supernatants (r=0.583, P<0.01).
Badou et al. (1997)IL-4T cellsIn Brown Norway rats, HgCl2 triggers early IL-4 mRNA expression both in vivo and in vitro by T cells, which may explain why autoreactive anti-class II T cells acquire a Th2 phenotype.
Szabo et al. (1997)interleukin-4T cellsGenes that regulate interleukin-4 expression in T cells.
Borody et al. (2002)IL-4T lymphocyteMETHODS: Parameters of host immunity were assessed as blood T lymphocyte production of interferon-gamma (IFN-gamma) and interleukin-4 (IL-4) being surrogate markers of mucosal Th1 and Th2 responses, respectively.
Shi et al. (2004)IL-4T lymphocytesThe production of IFN-gamma and IL-4 by T lymphocytes in the asthmatic model was inhibited significantly by dexamethasone.
Shi et al. (2004)IL-4T lymphocytesOBJECTIVE: To observe the production of IFN-gamma and IL-4 released in peripheral T lymphocytes in asthmatic patients and rat models and to clarify the dynamic changes of proliferation and differentiation of T lymphocytes during the progress of airway inflammation.
Noble and Kemeny (1995)IL-4T cellsPurified rat splenic CD8+ and CD4+ T cells and CD4-CD8- cells were stimulated with phorbol myristate acetate (PMA) and ionomycin for 6 h and expression of mRNA for IL-2, IL-4, IL-5, IL-6, IL-10 and IFN-gamma determined using a quantitative PCR technique.
Noble and Kemeny (1995)IL-4T cellsThe highest levels of IL-4, IL-5 and IFN-gamma mRNA were expressed by CD8+ T cells, the highest levels of message for IL-2 were expressed by CD4+ T cells and the highest levels of message for IL-6 and IL-10 by CD4-CD8- splenocytes.
Tori et al. (2000)IL-4T cellsThese RT6+NKT cells were predominantly CD4+ and showed significantly more expressions of intracellular IL-4 than the other T cells.
Charteris and Lightman (1994)interleukin 4T lymphocyteThe aim of this study was to investigate the T lymphocyte subsets involved in experimental autoimmune uveoretinitis (EAU) by quantifying the numbers of cells expressing mRNA for each of the lymphokines interferon gamma, interleukin 2, interleukin 4, and lymphotoxin throughout the disease process.
Gallo et al. (2008)interleukin-4T lymphocytesCytokine-producing T lymphocytes were identified within each T-lymphocyte subset as TH1 (interferon-gamma CD4), TH2 (interleukin-4 CD4), TC1 (interferon-gamma CD8), and TC2 (interferon-4 CD8) lymphocytes.
Fournié et al. (2002)IL-4T cellHg or Au act on the early steps of T cell activation resulting in IL-4 and IFNgamma gene expression with preferential IL-4 expression in BN rats.
Fournié et al. (2002)IL-4T cellsAnalyzing the effects of HgCl2 on T cells led us to identify a new signaling pathway implicated in IL-4 production.
Molet et al. (1999)IL-4T cellCONCLUSION: This study demonstrates that, in the CD4(+) T cell-driven LAR, the early production of IL-4, but not IL-5, by the transferred CD4(+) cells is essential for the development of the LAR.
Wu et al. (2004)IL-4T cellImmunohistochemical expressions of CXC chemokine receptor 3 (CXCR3) and CC chemokine receptor 4 (CCR4) were examined and compared with those of Th1 cytokine (interferon-gamma, IFN-gamma), Th2 cytokine (interleukin-4, IL-4), and anti-T cell antibody (W3/25).
Noble et al. (1993)IL-4T cellsCD4+ T cells isolated from these cultures also showed an increased capacity to secrete IL-4 and IL-5 when anti-IFN-gamma and IL-4 were present in the culture medium.
Noble et al. (1995)IL-4T cellsRat splenic CD8+ T cells expressed higher levels of IL-4, IL-5, IL-10, and IFN-gamma mRNA, as measured by reverse-transcription PCR, than CD4+ T cells from the same source, whereas CD4+ T cells expressed more IL-2 and IL-6 mRNA.
Mi and Zeng (2008)IL-4T cellsRESULTS: The levels of IL-4 and -6 mRNA transcripts were significantly higher in SLE T cells, as compared with that in the controls.
Sherman (2001)IL-4T cellsThe major IL-4 producers are CD4+ T cells, but the development of an IL-4-producing phenotype in these cells requires IL-4 signaling through the STAT6 pathway during differentiation.
Saoudi et al. (1995)IL-4T cellThe T cell lines produced interleukin (IL)-4 only or IL-4 and some interferon (IFN)-gamma and could, therefore, be considered as T helper type 2 (Th2) and Th0 cells, respectively.
Saoudi et al. (1995)IL-4T cellAn anti-ovalbumin T cell line that produced IL-4 and low amounts of IFN-gamma was used as a control and did not induce autoimmunity.
Gonzales et al. (2010)IL-4T cellsThis study aimed to test the functional effect of this specific genotype in AgP patients by analyzing gene expression of IL-4 and STAT6, and protein concentration of IL-4, in activated CD4+ T cells.
Caraher et al. (2000)IL-4T-cellsHere we report the optimisation of immunofluorescent staining for cell surface and intracellular antigens using three-colour flow cytometric analysis to measure the frequency of rat CD3(+)4(+) T-cells that produce IFN-gamma, IL-4 and IL-10.
Caraher et al. (2000)IL-4T-cellsIn vitro priming of splenic T-cells with antibodies against CD3 and CD28 and recombinant cytokines (IL-2 and IL-4) for 5 days followed by restimulation with PMA and ionomycin was required to stimulate cells to produce either IL-4 or IL-10.
Diaz-Sanchez et al. (1993)interleukin-4T cellsElimination of IgE regulatory rat CD8+ T cells in vivo differentially modulates interleukin-4 and interferon-gamma but not interleukin-2 production by splenic T cells.
Zhao et al. (1994)IL-4T cellS-Ag-specific T cell lines when stimulated with antigen expressed IL-2, IFN-gamma, and IL-4 mRNA, whereas only IL-2 and IFN-gamma could be detected in the supernatants.
Bode et al. (2007)IL-4T cellsWhen MAM was injected, the number of T cells in LEW.BN-4-10 rats producing T(h)2 cytokines such as IL-4 and IL-13 was significantly increased compared with LEW.
van Driel et al. (1999)IL-4T lymphocytesIn addition, colocalization was observed of CD3- and IL-4-positive T lymphocytes indicating that IL-4 production is mediated by T lymphocytes.
Mainou-Fowler et al. (2001)IL4T-cellsNo significant difference in the percentage (%) of T-cells that expressed cytoplasmic TNF alpha, IL4 and IL10 was observed between B-CLL and normal T-cells.
Mainou-Fowler et al. (2001)IL4T-cellsHowever, a significant increase in the mean level of intracellular TNF alpha and IL4 expression was observed in B-CLL compared with normal control T-cells (TNF alpha: P=0.031; IL4: P=0.0027).
Mainou-Fowler et al. (2001)IL4T-cellsThe differences observed with some B-CLL cases in the production of TNF alpha, IL4 and IL10 by peripheral blood T-cells may suggest survival mechanisms for the leukaemic cells in these patients.
Secrist et al. (1995)Interleukin 4T cellsInterleukin 4 production by CD4+ T cells from allergic individuals is modulated by antigen concentration and antigen-presenting cell type.
Secrist et al. (1995)IL-4T cellsWe have previously shown that CD4+ T cells from allergic individuals are predisposed to produce interleukin (IL)-4 in response to allergens, and that allergen immunotherapy greatly reduced IL-4 production in an allergen-specific fashion.
Secrist et al. (1995)IL-4T cellsThe mechanism that results in the reduction of IL-4 synthesis in treated individuals is unknown, but because clinical improvement during immunotherapy is associated with the administration of the highest doses of allergen, we hypothesized that high concentration of allergen results in the downregulation of IL-4 synthesis in CD4+ T cells.
Secrist et al. (1995)IL-4T cellsIn this report, we demonstrated that CD4+ T cells from allergic donors produced high levels of IL-4 when stimulated with low concentrations of allergen (0.003-0.01 micrograms/ml), particularly when B cell-enriched populations presented the antigen.
Secrist et al. (1995)IL-4T cellsThe quantity of IL-4 produced was also found to be inversely related to the extent of proliferation of the CD4+ T cells in response to allergen/antigen; maximal proliferation of CD4+ T cells occurred in response to high concentrations of antigen when IL-4 production was minimal.
Secrist et al. (1995)IL-4T cellMoreover, the addition of increasing numbers of APC (either B cells or monocytes) to cultures containing a constant number of responder T cells resulted in increased T cell proliferation and decreased IL-4 production.
Secrist et al. (1995)IL-4T cellsThese results indicate that the circumstances under which memory T cells are activated, as well as the strength of the proliferative signal to T cells, greatly affect the quantity of IL-4 produced.
Röcken et al. (1994)IL-4T cellHowever, the mechanisms that regulate production of IL-4 or other T cell lymphokines in vivo remain unknown.
Röcken et al. (1994)IL-4T cellsSEA-specific CD4+ T cells produced large amounts of IL-4 when restimulated within 10 d after in vivo priming.
Röcken et al. (1994)IL-4T cellsAlthough controversy exists regarding the susceptibility of Th2-like cells to tolerogenic signals, high doses of superantigen readily abolished the capacity to produce IL-4 in both naive T cells and in T cells already primed for IL-4 production.
Röcken et al. (1994)IL-4T cellInfection with the nematode, Nippostrongylus brasiliensis, reversed the established T cell tolerance, whereas the signals which induced IL-4 production in normal T cells, antigen and IL-4, were not capable of reversing superantigen-specific tolerance in vivo.
Ohga et al. (2008)IL-4T cellsYM-341619 suppressed the production of IL-4 and the expression of GATA-3 mRNA, a Th2 transcription factor, in T cells cultured with anti-CD3 antibody and anti-CD28 antibody in the presence of IL-4.
Dean et al. (2002)IL4T cellsFurther study of patients producing high levels of IL4 (about one third of the patients) indicated that they had a lower percentage of alphabeta+ T cells in the double negative compartment than did patients with fewer IL4 containing cells.
Mainou-Fowler et al. (2004)IL4T-cellThe present study examines the production of IL4 by 2-day phytohaemaglutinin (PHA)-stimulated peripheral blood (PB) cells in HL and correlates the cytokine levels with the proportion of the different T-cell sub-populations.