Viewing negative mentions of gene expression of IL2: interleukin 2 (Homo sapiens)

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Nutman et al. (1987)interleukin 2In marked contrast, when antigen-induced lymphokine production was examined, most patients with microfilaremia were unable to produce either interleukin 2 (IL-2) or gamma-interferon (i.e., were nonresponders), and the few who could (hyporesponders, generally with quite low microfilaremia levels) did so at levels significantly less than those of patients with elephantiasis, all of whom showed strong responses to parasite antigen.
Gulino et al. (1990)IL-2HeLaConstitutive expression of IL-2 mRNA in human IL-2 gene-transfected NIH-3T3 and HeLa cells was associated to the secretion of bioactive IL-2 protein, while no IL-2 production was observed in untransfected or vector-transfected cells.
Blumberg et al. (1987)IL-2lymphocytesCytotoxic activity correlated positively with the number of T4 lymphocytes (r = .56, P less than .001) and the amount of IL-2 produced (r = .47, P less than .01) but not with interferon production.
Toossi et al. (1989)IL-2Leu 11-enriched cells did not express high affinity IL-2 receptors nor did they deplete IL-2 activity from culture media.
Manger et al. (1986)IL-2After treatment with 5-azacytidine, HUT 78 cells produced maximal levels of IL-2 in response to PMA alone without requiring [Ca++]i increasing stimuli.
Liu and Rosenberg (2001)IL-2PBMCs similarly transduced with a control vector did not produce IL-2 and failed to proliferate in the absence of IL-2.
Tartour et al. (2000)IL-2 mRNAbloodIndeed, exogenous IL-2 mRNA could no longer be detected in the peripheral whole blood 48 hours after Vero-IL-2 cell administration.
Packard (1990)IL-2However, IL-2 is not normally synthesized by solid tumor cells.
Müller-Quernheim et al. (1986)IL 2 geneT cellsThe sarcoid lung T cells, however, did not express the IL 2 gene constitutively; when placed in culture with no stimulation and evaluated after 24 hr, they demonstrated down regulation of the amounts of IL 2 mRNA transcripts, despite the fact that they were capable of re-expressing the IL 2 gene and releasing more IL 2 in response to added activation signals.
Visani et al. (1987)IL2Noninduced cells did not express IL2 receptors.
Lin et al. (1982)IL2oocytesIncubation medium of oocytes injected with RNA from another T-cell line, 6G1, which does not produce IL2, did not contain DNA-stimulating activity.
Bettens et al. (1984)IL 2Furthermore, anti-Tac can inhibit the mitogenic signal given by endogenous IL 2, but not by in situ produced IL 2, an observation of importance to further investigations of the mechanisms by which IL 2 interacts with specific receptors to elicit proliferation.
Waldmann et al. (1985)IL-2T-cellsResting T-cells do not express IL-2 receptors, but receptors are rapidly expressed on T-cells following interaction of antigens, mitogens, or monoclonal antibodies with the antigen-specific T-cell receptor complex.
Waldmann et al. (1985)IL-2T-cellNormal resting T-cells and most leukemic T-cell populations do not express IL-2 receptors; however, the leukemic cells of the 11 patients examined who had human T-cell lymphotropic virus-associated adult T-cell leukemia expressed the Tac antigen.
Mills et al. (1990)IL2IL2RIL2 induced tyrosine phosphorylation in YT 2C2 cells which express only the 75-kDa intermediate affinity IL2 binding molecule (IL2R beta) but not in cells which either express only the 55-kDa low affinity IL2 receptor molecule (IL2R alpha) or no IL2-binding sites.
Benary et al. (2008)IL-2regulatory T cellsIn contrast, regulatory T cells (T(reg) cells) do not express IL-2, although they are activated via the same pathways.
Philip et al. (1996)IL-2Transfections with the non-AAV plasmid containing the identical expression cassette as the AAV plasmid induced IL-2 expression in the tumor cell line but failed to produce IL-2 in primary tumor cells.
Wiskocil et al. (1985)IL 2T cellIn the resting state, the T3-positive, human T cell line Jurkat does not synthesize detectable amounts of either interleukin 2 (IL 2) or gamma-interferon (IFN-gamma).
Miedema et al. (1985)IL2Moreover, the T8+ cells produced IL2, despite their failure to help Ig synthesis.
Waldmann et al. (1984)IL-2T cellsResting T cells do not express IL-2 receptors but receptors are rapidly expressed on T cells following the interaction of antigens, mitogens, or monoclonal antibodies with the antigen specific T-cell receptor complex.
Waldmann et al. (1984)IL-2T-cellNormal resting T cells and most leukemic T-cell populations do not express IL-2 receptors however the leukemic cells of all patients with human T-cell leukemia/lymphoma virus (HTLV-I) associated, adult T-cell leukemia (ATL) examined expressed the Tac antigen.
Zicari et al. (1995)IL-2Immunoreactive IL-2 was detected only in AF samples obtained from women of Groups 1 and 3; the higher concentration was found in Group 1 samples; IL-2 was not detected in AF samples from women of Group 2.
Salmon et al. (1988)IL-2The helper cells produced no detectable IL-2 and proliferated poorly.
Watanabe (1986)IL2thymocytesThey were as follows; (1) mature T lymphocytes, fraction I and II, which produced IL2, responded to IL2 and had both suppressor and helper function; (2) immature thymocytes, fraction III and a part of IV, which responded to IL2 without production of IL2 and had neither suppressor nor helper function, but suppressor function was induced by Con A with IL2 from this group of thymocytes; (3) immature thymocytes, a major part of fraction IV, which had no IL2 production and no responsibility, and had neither suppressor nor helper function.
Sidell and Ramsdell (1988)IL-2PBLIn contrast to these effects on thymocytes, peripheral blood lymphocyte (PBL) proliferative responses were unaffected by RA treatment and, correspondingly, RA affected neither IL-2 receptor expression on PBL blasts nor the growth of these cells.
Teodorczyk-Injeyan et al. (2008)IL-2Seven (2 each in VC and SMT-NC and 3 in SMT-C group) of 76 subjects investigated in this study demonstrated no detectable IL-2 synthesis in unstimulated PBMC cultures both prior to and post-intervention.
Waldmann (1987)IL-2T cellsResting T cells do not express IL-2 receptors, but receptors are rapidly expressed on T cells following interaction of the antigen-specific T-cell receptor complex with appropriately processed and presented antigens.
Waldmann (1987)IL-2T-cellNormal resting T cells and most leukemic T-cell populations examined did not express IL-2 receptors; however, the leukemic cells of all patients with human T-cell lymphotrophic virus (HTLV-I)-associated adult T-cell leukemia (ATL) expressed the Tac antigen.
Venuta et al. (1983)IL-2DaudiSince normal T cells do not require Daudi to produce IL-2 and since Daudi cells do not produce IL-2 under any conditions, we conclude that the cell responsible for IL-2 production in acute lymphatic leukemia is a leukemic T cell with an altered mechanism of IL-2 production at the level of the Pan T2 binding site.
Haaff et al. (1986)IL-2bladderNo IL-2 was detected in specimens obtained prior to BCG instillation or from donors with no detectable bladder pathology.
Dohlsten et al. (1988)IL2While the CD4+45R- subset produced maximal amounts of IL2 within 24 h and IFN-gamma within 72 h, the CD4+45R+ subset produced no IL2 within 24 h and merely marginal amounts of IFN-gamma as assayed after 24 to 96 h.
Chen et al. (1985)interleukin 2MLA 144The gibbon leukemia cell line MLA 144 differs from every other T-lymphocyte line in that it constitutively makes interleukin 2 (IL-2) (also called T-cell growth factor) without stimulation by antigen, lectin, or tumor promoters.
Miller et al. (1986)IL 2Although these cells have been activated to an IL 2R+ stage, most of them are unable to produce detectable IL 2 unless they are cultured together with the original, activating antigen.
Salmeron et al. (1992)IL-2TILUncultured TIL produced interferon gamma (IFN gamma), but not interleukin-2 (IL-2) or IL-4, in response to anti-CD3 mAb or IL-2.
Brunvand et al. (1993)IL-2T cellTo determine if the trans-activators documented in T cell nuclear extracts actually bind the IL-2 promoter in vivo, ligation mediated PCR (LMPCR) genomic footprinting was performed on the IL-2 promoter in both activated and non-activated T cells and HL60 promyelocytes, which do not express the IL-2 gene.
Schiphorst et al. (1999)IL-2IL-2 could not be detected in haemo- or peritoneal dialysates.
Miller and Olds (1987)IL-2Cultures from children with either active lupus or the pneumonia of cystic fibrosis produced very little IL-2, but cultures from children with arthritis produced apparently normal amounts.
Hori et al. (1986)IL-2Leukemic cells from 7 ATL patients examined showed no or very poor proliferative response to IL-2 though they expressed IL-2 receptors without any stimulation.
Lopez et al. (1998)IL-2T cellThese findings show that human breast cancer tumor-induced repression of IL-2 RNA translation is the basis of failure of TIL to express the IL-2 receptor and subsequent T cell hyporesponsiveness.
Morita et al. (1992)IL-2IL-2 and IL-4 were not detected in any cases tested.
Kumagai et al. (1988)IL-2Competence is defined as the ability to subsequently proliferate in response to exogenously added IL-2 or PDB in the second phase of the culture, but not to synthesize IL-2 or proliferate without these additions.
Schwab et al. (1990)IL-2T cellsHowever, T cells from old donors that do not express high affinity IL-2 binding sites express both the IL-2 binding 55 and 75 kd chains.
Dohlsten et al. (1988)IL-2In contrast, the Leu 3+18+ subset produced no IL-2 after 24 h, and maximal amounts of IL-2 no sooner than 48 h of culture, and only small amounts of IFN-gamma during the entire culture period of 96 h.
Bulmer and Johnson (1986)IL-2deciduaInfiltrating T lymphocytes in early pregnancy decidua do not express the IL-2 receptor.
Kwong and Teh (1987)IL-2spleenASHF did not increase the amount of IL-2 produced by irradiated B6 spleen cells but increased the amount of IL-2 produced in CBA anti-B6 cultures by 3.8-fold.
Nakamura et al. (1991)IL-2T cellsIn accordance with the magnitude of IL-2 responsiveness, freshly isolated CD8+ T cells expressed significant amounts of p75 IL-2 receptor, while fresh CD4+ T cells did not express p75 IL-2 receptor.
Krutmann et al. (1990)interleukin-2monocytesHowever, T cells failed to produce interleukin-2 above background levels when they were placed in culture with monocytes exposed to UVB doses as low as 50 J/m2.
Pawelec et al. (1982)IL2CCRF-CEMMOLT4, HSB2, RPMI1301, and CCRF-CEM all failed to produce detectable levels of IL2.
Pawelec et al. (1982)IL2A second, JM, failed to produce any IL2, while the third, JHAN, produced intermediate levels.
Hurez et al. (2003)IL-2T cellsT cells destined to produce IL-2 required prolonged interactions with DCs, whereas most T cells established only transient interactions with DCs and were activated, but did not express IL-2.
Benson and Ziegler (1989)IL-2macrophagesAlthough antigen-specific T cells could bind to drug-treated macrophages, they did not produce IL-2.
Kato et al. (1997)IL-2They did not produce IL-2 by stimulation with PHA and phorbol myristate acetate (PMA).
Kirkpatrick et al. (1985)IL-2Asymptomatic heterosexual and homosexual subjects produced comparable amounts of IL-2, but 8 of 11 AIDS patients with opportunistic infections and two of three AIDS patients with both opportunistic infections and Kaposi's sarcoma failed to produce detectable amounts of IL-2; all seven AIDS patients with only Kaposi's sarcoma produced IL-2.
Kirkpatrick et al. (1985)IL-2T-cellThis observation is interpreted as indicating that failure to produce IL-2 by AIDS patients is most likely due to depletion of IL-2-producing cells, although an abnormality of T-cell function has not been excluded.
Kirkpatrick et al. (1985)IL-2In addition, three of eight AIDS patients who did not produce IL-2 produced supernatants that inhibited growth of IL-2-dependent cells in the presence of IL-2.
Palacios (1984)IL 2T cellsThe capacity for circulating human T cells which have or lack receptors for interleukin 2 (IL 2) to produce IL 2, interleukin 3 (IL 3), and interferon-gamma (IFN-gamma) under the stimulus of phytohemagglutinin was studied.
Palacios (1984)IL 2T cellsTac- T cells produced IL 2 and IL 3 but not IFN-gamma.
Grabstein et al. (1986)IL 2T lymphocytesIn populations of peripheral blood T lymphocytes, the production of IL 2 and IFN-gamma were not necessarily coordinately expressed.
Hovdenes and Egeland (1987)IL-2IL-2 was not detected in unstimulated cell-culture supernatants from any of the four RA patients tested, but it was present in small amounts in supernatants of unstimulated cultures derived from three of seven JRA patients studied.
Wakasugi et al. (1985)IL 2T cellsPurified T cells incubated for 3 days with PHA neither proliferate nor express IL 2 receptors as detected by FACS analysis with the use of anti-Tac antibodies.
Mark and Mangkornkanok-Mark (1985)interleukin-2macrophagesAn interlocking system wherein the T4 (helper) cells do not synthesize interleukin-2 to generate the cytotoxic cells due to the lack of functional thymosin as well as the lack of interleukin-1 from the macrophages is postulated.
Tsutsui et al. (1991)IL-2However, the other four helper-type clones did not produce IL-2 by such stimulation, although they continued the production of IL-4.
Reem and Yeh (1985)IL 2thymocytesThe results indicate that thymocytes cultured in complete medium do not express receptors for IL 2, nor did IL 2 by itself induce the expression of IL 2 receptors.
Ballmer-Weber et al. (1995)IL-2plasmaIL-2 and IL-6 were not detectable in the plasma prior to IL-2 injections and increased to 549 +/- 315 U ml-1 (P < 0.001) and 7 +/- 6 pg ml-1 (P < 0.01), respectively, after IL-2 administration.
Sieg et al. (2001)IL-2T-cellSubnormal T-cell production of interleukin-2 (IL-2) in human immunodeficiency virus (HIV) disease has been described; however, it is not clear whether failure to synthesize IL-2 represents a selective or global defect in T-cell cytokine production.
Vyth-Dreese and De Vries (1984)IL-2monocytesIn contrast to normal T cells, the T-PLL cells could not be induced to proliferate by mitogenic lectins or alloantigens in the presence or absence of human interleukin-1 (IL-1), interleukin-2(IL-2) or allogeneic monocytes and did not produce IL-2.
Waldmann (1986)IL-2T-cellResting T cells do not express IL-2 receptors, but receptors are rapidly expressed on T cells following interaction of the antigen-specific T-cell-receptor complex with appropriately processed and presented antigens.
Waldmann (1986)IL-2T-cellNormal resting T cells and most leukemic T-cell populations examined did not express IL-2 receptors; however, the leukemic cells of all patients with human T-cell lymphotrophic virus (HTLV-I)-associated adult T-cell leukemia (ATL) expressed the Tac antigen.
Siegel et al. (1987)IL-2Yet, prior to activation, these cells do not express IL-2 receptors recognized by monoclonal antibodies to the Tac antigen.
Matsuzaki et al. (1986)IL-2T-cellThe T-cell hybridomas became infected but did not produce IL-2 unless simultaneously exposed to syngeneic I-A+ antigen-presenting cells.
Matsuzaki et al. (1986)IL-2T-cellIn this situation, the persistently infected T-cell hybridomas produced IL-2 without being reexposed to virus.
Murakawa et al. (1985)IL 2T cellsSLE T cells failed to produce normal levels of IL 2 in vitro compared with normal control T cells.
Welte et al. (1987)IL-2T cellsThis IL-2 was not produced by Leu-11+ natural killer cells, which were found to be predominant in the circulation at this time, but by T11+, T3+, Ia antigen-bearing T cells.
Pinkerton et al. (2008)IL2Genes that were not expressed included: (1) the immunoregulatory lymphokines (IL2-IL5), IL17 and IL17B; (2) IL10 and other members of the IL-10 family of anti-inflammatory cytokines (IL19, IL20, IL22 and IL24); and (3) TNF and RANKL.
Ge et al. (2004)IL-2Investigation of IL-15 receptor expression using RT-PCR showed that IL-15Ralpha and IL-2Rbeta chains but not IL-2Ralpha or gamma chain were constitutively expressed in these cells.
Bykovskaia et al. (1999)IL-2Isolated CD34+ cells did not express IL-2R subunits but after 2-3 days in culture with IL-15, they were found to express IL-2Rgamma.
Dadaglio et al. (1994)interleukin 2T cellsThe V beta 8 anergic T cells did not express interleukin 2 receptors (IL-2Rs) and failed to proliferate in response to exogenous IL-2 or IL-4, suggesting that this anergy was not a reversible process, at least by the use of these cytokines.
Tinubu et al. (1994)IL-2T cellsIL-2Rs are expressed by T cells activated in response to foreign histocompatibility Ags but not by normal cells.
Brown et al. (1991)interleukin 2T cellsHigh-affinity interleukin 2 receptors (IL-2Rs) are expressed by T cells activated in response to foreign histocompatibility antigens but not by normal resting T cells.
Paglieroni et al. (1988)IL2Cells from patient 1 coexpressed CD5 (leu-1), CD19 (Leu-12), CD20 (B1), and HLA-DR; they were CD10 (J5), CD21 (B2), CD22 (Leu-14), CD25 (IL2-R1), PCA-1, surface, and cytoplasmic Ig negative.
Maggi et al. (1988)IL 2T cellAlthough 21 out of 503 (4%) CD4+ T cell clones produced IL 4, but not IFN-gamma or IL 2, and 208 (41%) produced IL 2 and/or IFN-gamma, but not IL 4, a total number of 185 (37%) CD4+ clones showed the ability to produce IL 4 plus IL 2 and/or IFN-gamma.
Pistoia et al. (1986)IL-2LGLLGL proliferated in response to rIL-2 but did not express detectable IL-2 receptors, even after prolonged periods of culture.
Casoli et al. (2000)IL-2Endogenous IL-2 was not produced, whereas IL-6 and TNF-alpha were secreted at levels compatible with their known ability to up-regulate HIV-1 expression.
Harel-Bellan et al. (1986)IL 2bloodSurprisingly, cells from this Percoll fraction examined immediately after separation from the blood do not express detectable amounts of IL 2 receptors as assessed by three different techniques: binding of [3H]IL 2, binding of [125I]anti-Tac antibodies, and FACS analysis with the use of anti-Tac antibodies.
Asao et al. (1994)IL-2T cellWe have investigated the role of JAK3 in interleukin 2 (IL-2)-induced signal transduction with a human T cell line, ED40515(-), lacking expression of the IL-2 receptor gamma chain and its sublines transfected with wild-type or mutant cDNAs of the IL-2 receptor gamma chain.
Schauf et al. (1993)IL-2 mRNAPBMCPBMC of 7 patients (all of those with systemic symptoms) expressed interferon-gamma mRNA and none expressed IL-2 mRNA.
Loertscher et al. (1988)interleukin 2In the presence of Ts, most MLR responder cells did not express interleukin 2 receptors, although a small minority of cells constantly escaped Ts inhibition.
Ratliff et al. (1986)Interleukin-2bladderInterleukin-2 (IL-2) was detected in urine but not serum of patients receiving intravesical bacille Calmette-Guerin (BCG) therapy for bladder cancer.
Konstantinidis et al. (2005)IL-2NK-92IL-2 was not detected in the supernatant and growth of non-IL-2-modified NK-92 cells was not supported during coculturing experiments.
Vyth-Dreese and de Vries (1983)IL-2These continuously proliferating T-CLL cells failed to produce IL-2 constitutively.
Kumaki et al. (1995)IL-2Using Epstein-Barr virus-transformed B-lymphoblastoid cell lines (B-LCLs) established from two unrelated XSCID patients, we could show that neither expressed the IL-2 receptor gamma chain on the cell surface.
Chopra et al. (1989)IL-2T cellsPhorbol myristate acetate and calcium ionophore A23187-stimulated human T cells do not express high-affinity IL-2 receptors.
Oh-Hori et al. (1990)IL-2-dependentT-cellUsing this antiserum, we show that HTLV-I-positive T-cell lines, whether they are IL-2-dependent or not, scarcely express p56lck.