Viewing affirmative mentions of transcription of IL2: interleukin 2 (Homo sapiens)

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Weaver et al. (2007)IL-2 geneTranscription of the IL-2 gene is tightly controlled by the combined actions of multiple transcriptional activators.
Weaver et al. (2007)IL-2However, the contribution of sequences in the IL-2 core promoter and the architecture of the IL-2 regulatory region to setting levels of IL-2 transcription are not understood.
Yamamoto et al. (1985)IL2lymphocytesInduction of interleukin 2 (IL2) mRNA synthesis in human tonsillar lymphocytes was studied by quantifying the relative levels of IL2 mRNA in the lymphocytes stimulated under various conditions by the dot hybridization method.
Landsverk et al. (2002)IL-2 geneT-cellThe normally repressed IL-2 gene is transcribed in nuclei from quiescent human T cells and from various non-T-cell lines.
Pahlavani and Richardson (1996)IL-2T cellsThe age-related decline in IL-2 production has been shown to arise from a decline in IL-2 transcription, and a recent study suggests that the transcription factor NFAT (nuclear factor of activated T cells) may play a role in the decline in IL-2 transcription.
Pinkston et al. (1987)IL 2lungFurthermore, Northern analysis of lung T cell RNA before therapy demonstrated IL 2 mRNA transcripts, whereas no IL 2 transcripts were observed during therapy.
Dautry-Varsat et al. (1988)IL-2T-cellCsA also prevents the constitutive secretion of IL-2 in this T-cell line by blocking transcription of the IL-2 gene.
Henics et al. (1994)IL-2Six major cytoplasmic proteins bound in vitro transcribed RNA probes containing sequences from the 3'-UTR of normal human IL-2 (3'-IL-2), GM-CSF (delta 2R1), and the virally altered MLA IL-2 (3'-IL-2 PV) mRNA.
Aoki et al. (1997)IL-2AECTranscriptional regulation of IL-2 was investigated with a transgenic human AEC line, 16HBE/IL-2 luciferase; there is constitutive IL-2 transcription at rest, and IL-2 transcription is enhanced 8-fold by PMA and 25-fold by PMA + histamine.
Sawami et al. (1992)IL-2Addition of IL-2 also increased mRNA levels for c-fos, c-myc, IL-2 receptor alpha, and IL-2 receptor beta chain.
Nordmann et al. (1989)interleukin 2Cyclosporin A was used as a valuable tool to gain more insight into the quantitative aspects of interleukin 2 production, on the basis of the assumption that transcription of the interleukin 2 gene is completely inhibited shortly after administration of cyclosporin A.
Hess et al. (1997)interleukin-2proximalSMX-HA at immunosuppressive concentrations also failed to interfere with interleukin-2 mRNA transcription and interleukin-2 protein production, which suggests that signaling events proximal to cytokine production are not affected by the metabolite.
Chen (2001)interleukin-2T cellTriptolide inhibits both Ca(2+)-dependent and Ca(2+)-independent pathways and affects T cell activation through inhibition of interleukin-2 transcription at a site different from the target of cyclosporin A.
Efrat and Kaempfer (1984)IL-2oocytesThe regulation of human interleukin 2 (IL-2) mRNA production in induced normal lymphocytes was studied by following the expression of isolated mRNA in microinjected oocytes of Xenopus laevis.
Ramarli et al. (1987)IL-2thymocyteHere we show that, within the T3+ thymocyte compartment, T3-Ti crosslinking rapidly inhibits T11-initiated interleukin 2 (IL-2) gene transcription and translation.
Ramarli et al. (1987)IL-2IL2RThis inhibition is restricted to the IL-2 gene (IL2) as transcription of both the IL-2 receptor gene (IL2R) and the Ti beta-chain gene (TCRB) are not affected (human gene designations are in parentheses).
Ramarli et al. (1986)IL-2thymocyteHere we show that, within the T3+ thymocyte compartment, T3-Ti crosslinking rapidly inhibits T11-initiated interleukin 2 (IL-2) gene transcription and translation.
Ramarli et al. (1986)IL-2IL2RThis inhibition is restricted to the IL-2 gene (IL2) as transcription of both the IL-2-receptor gene (IL2R) and the Ti beta-chain gene (TCRB) are not affected (human gene designations are in parentheses).
Müller-Quernheim et al. (1986)IL 2bloodIn contrast, no IL 2 mRNA transcripts could be detected in fresh autologous blood T cells or in purified autologous blood Leu-3+ T cells, although IL 2 mRNA transcripts were inducible in these cells by phytohemaglutinin/phorbol myristic acetate.
Modiano et al. (1991)IL2 geneHowever, when PKC inhibitors were added after IL2 gene transcription had already proceeded for 3-4 h, the IL2 in the culture supernatants was still reduced by 30-80%, and intracellular IL2 was increased by up to 50%.
DiSanto et al. (1990)IL-2T cellThis patient's immunological defect appears to be attributable to a selective deficiency in T cell production of IL-2, which may reflect a subtle abnormality in the IL-2 gene locus or a defect in a regulatory factor necessary for IL-2 transcription.
Briggs et al. (1999)interleukin-2mononuclear cellsTo evaluate molecular mechanisms that might account for the heterogeneity in the in vitro responsiveness of individual subjects' peripheral blood mononuclear cells (PBMC) to immunosuppressive drugs, the authors quantitated in normal human cells the suppressive effects of the glucocorticoids prednisolone and methylprednisolone and of cyclosporine on interleukin-2 (IL-2) mRNA expression and IL-2 production, as well as the stimulatory effect of these drugs on IkappaBalpha mRNA expression.
Hopkins and Failla (1999)IL-2 geneSynthesis of this cytokine is regulated by alterations in transcription of the IL-2 gene and the stability of IL-2 mRNA.
Hopkins and Failla (1999)IL-2 geneT lymphocytesThese data indicate that decreased cellular Cu attenuates IL-2 synthesis in T lymphocytes by inhibiting transcription of the IL-2 gene.
Krönke et al. (1984)TCGFTo define further both the specificity of CsA and the level at which it interferes with lymphokine gene expression, we have studied its effects on TCGF mRNA accumulation as well as TCGF gene transcription.
Krönke et al. (1984)TCGFThese data suggest a relatively selective action of CsA on TCGF gene transcription.
Waldmann et al. (1985)IL-2T-cellIn human T-cell lymphotropic virus-I infected cells, the Mr 42,000 long open reading frame protein encoded in part by the pX region of this virus may act as a transacting transcriptional activator that induces IL-2 receptor gene transcription, thus providing an explanation for the constant association of IL-2 receptor expression with adult T-cell lymphotropic virus-I infection of lymphoid cells.
Baumann et al. (1991)IL-2T-cellThe structurally unrelated immunosuppressive drugs cyclosporin A (Sandimmun) and FK-506 both interfere with the process of T-cell proliferation by blocking the transcription of the T-cell growth factor interleukin-2 (IL-2).
Ohmura and Onoue (1990)IL-2 cDNAlymphocytesFurther study demonstrated that the rate of degradation of 32P-labeled IL-2 mRNA, which was prepared by cell-free transcription of IL-2 cDNA, in the polysomal fraction obtained from PDB-stimulated lymphocytes was decreased compared with that obtained from unstimulated lymphocytes.
Levi-Schaffer et al. (1996)IL-2eosinophilsIn addition, eosinophils have been shown to transcribe messenger RNA for IL-2.
Waldmann et al. (1984)IL-2In HTLV-I infected cells the 42 kdalton long open reading frame (LOR) protein encoded in part, by the pX region of HTLV-I may act as a transacting transcriptional activator that induces transcription of the IL-2 receptor gene thus providing an explanation for the constant association of HTLV-I infection of lymphoid cells and IL-2 receptor expression.
Yao et al. (2003)IL-2parenchymaHuman IL-2 mRNA was detected by in situ hybridization in the spinal pia mater and parenchyma of the lumbar, sacral, thoracic and cervical regions, and gray matter had higher level of IL-2 expression than white matter.
Wieder et al. (1990)IL-2T-cellsTo amplify very low levels of IL-2 mRNA, sequential reverse transcription and polymerase chain reaction (RT-PCR) of T cell mRNA were used to demonstrate the capacity of the calcium signal (ionomycin) to promote low-level IL-2 transcription in normal human T-cells without additional signals.
Ferguson et al. (2001)IL-2 geneT-cellShortly after T-cell stimulation, transcription of the IL-2 gene is upregulated.
Ferguson et al. (2001)IL-2 promoterHere, we studied the kinetic mechanism of basal transcription at the IL-2 promoter using a human in vitro RNA polymerase II transcription system.
Wilkinson and Euhus (2001)IL-2The immunosuppressive drug CsA is known to block native IL-2 transcription but has no affect on the adenoviral-mediated IL-2 or lac-Z transgene.
Waldmann (1987)IL-2In HTLV-I-infected cells, the 42-Kd long open reading frame (tat) protein encoded in part by the tat region of HTLV-I may act as a transacting activator that induces transcription of the IL-2 receptor gene, thus providing an explanation for the constant association of HTLV-I infection of lymphoid cells and IL-2 receptor expression.
Hallet et al. (1989)IL-2 geneA combination of phorbol ester plus calcium ionophore was very efficient in all clones tested (17/17) in inducing transcription of the IL-2 gene.
Oh et al. (1993)IL-2lymphocyteConcomitant with the inhibition of lymphocyte activation and interleukin 2 (IL-2) production, transcription of the IL-2 message is also reduced in a time-dependent manner.
Tessier et al. (2002)interleukin-2T-cellWe assessed the implication of these PLA(2) isoforms in transcription of the interleukin-2 (IL-2) gene, involved in T-cell proliferation.
Boumpas et al. (1991)IL-2 geneNuclear transcription run-off assays showed that high doses of dexamethasone inhibited the transcription of the IL-2 gene but not that of the IL-2R gene.
Boumpas et al. (1991)IL-2 geneDexamethasone both inhibits transcription of the IL-2 gene and decreases the stability of IL-2 mRNA.
Falsafi-Amin et al. (2008)interleukin-2bloodThe aim pf the present study was to characterize the time course of the synthesis of mRNA encoding interleukin-2 (IL-2) by peripheral blood mononuclear cells (PBMCs) isolated from nickel-allergic women and subsequently exposed to nickel sulphate in vitro.
Krönke et al. (1985)IL-2T cellsHowever, mitogenic stimuli activate IL-2 receptor gene expression in normal T cells, whereas these stimuli paradoxically inhibit IL-2 receptor gene transcription in HTLV-I-infected leukemic T cells.
Bunting et al. (2006)interleukin-2Control of interleukin-2 gene transcription: a paradigm for inducible, tissue-specific gene expression.
Bunting et al. (2006)IL-2 geneThe mechanisms that control IL-2 gene transcription have been studied in detail for the past 20 years and our current understanding of the nature of the inducible and tissue-specific controls will be discussed.
Becker et al. (1995)IL-2T cellsAnergy is a mechanism of T-lymphocyte tolerance induced by antigen-receptor stimulation in the absence of costimulation, whereby T cells exhibit a defect in antigen-induced transcription of the interleukin 2 (IL-2) gene.
Granelli-Piperno (1992)IL-2T lymphocytesCyclosporin A (CsA) and FK506 inhibit the production of IL-2 in T lymphocytes at the level of gene transcription.
Evans et al. (1992)IL-2T-cellFrom these data we conclude that, in human T-cells, RB phosphorylation is not directly associated with T-cell receptor-mediated events, but requires the interaction of IL-2 and new gene transcription following IL-2 stimulation.
Shaw et al. (1987)IL2CsA interrupted ongoing transcription of IL2 by a mechanism not dependent on the induction of a new protein.
Cullen (1986)IL-2In the absence of the trans-activating viral gene product, tat-III, IL-2 transcripts specific for the HIV LTR were present in low abundance in transfected cells and showed a low translational efficiency, when compared with IL-2 mRNAs transcribed from other viral promoters.
Cullen (1986)IL-2Coexpression of tat-III resulted in a marked increase in the steady state level of IL-2 mRNAs transcribed from the HIV LTR, and these mRNAs also demonstrated a specific enhancement of their translational efficiency.
Kudla et al. (2006)IL2We have shown that the efficiency of mRNA production from GFP, IL2, and Hsp70-family genes in mammalian cells correlates with the silent-site GC content of these genes.
Umadome et al. (1988)IL-2Although the expression of IL-2 or IL-4 mRNA was not detected in fresh leukemic cells, the expression of IL-2 mRNA, but not IL-4 mRNA, was induced by phytohemagglutinin stimulation, and the leukemic cells proliferated.
Krönke et al. (1985)IL-2T cellsAlthough silent in resting T cells, the genes encoding c-myc and the interleukin 2 (IL-2) receptor were induced early, preceding gamma interferon (IFN-gamma), IL-2, and transferrin receptor gene transcription.
Hurez et al. (2003)IL-2T cellThus, there is intrinsic variability in clonal T cell populations that restricts IL-2 commitment, and prolonged engagement with mature DCs is necessary, but not sufficient, for IL-2 gene transcription.
Lewis et al. (1993)IL-2Effects of rolipram and CI-930 on IL-2 mRNA transcription in human Jurkat cells.
Lewis et al. (1993)IL-2IL-2 transcription was greatly inhibited by 1 microM CsA, whereas neither 10 microM rolipram nor 10 microM CI-930 had any effect on steady-state levels of IL-2 mRNA.
Lewis et al. (1993)IL-2T cellTherefore, the mechanism by which inhibitors of PDE-III and PDE-IV affect T cell proliferation is not likely to involve suppression of IL-2 mRNA transcription.
Tendler et al. (1990)interleukin 2mononuclear cellsBy using a modification of the polymerase chain reaction, we compared the levels of interleukin 2 (IL-2) and IL-2 receptor alpha chain (IL-2R alpha) mRNA expression to the transcription of the HTLV-I transactivator gene, pX, in peripheral blood mononuclear cells of HAM/TSP and ATL patients as well as seropositive carriers.
Tendler et al. (1990)IL-2circulating cellsDespite markedly elevated levels of soluble IL-2R alpha in ATL, transcripts for IL-2 and pX were not demonstrable in the circulating cells.
Mouzaki et al. (1993)IL-2T cellsIn the present study, analysis of the interleukin-2 (IL-2) gene activation shows that naive human helper T cells (cord blood CD4+ T cells, adult CD4+CD45RO- T cells) regulate IL-2 transcription by a mechanism involving both a silencer and an activator acting on the purine-rich IL-2 promoter elements (NF-AT binding sites).
Mouzaki et al. (1993)IL-2memory T cellsTheir IL-2 transcription seems to be controlled solely by the transition from inactive to active functional state of a positive transcription factor binding to these promoter elements as well as its cytoplasmic or nuclear location: in resting memory T cells the activator is located in the cytoplasm and is inactive, whereas in stimulated cells it is functional in promoting transcription and now resides in the nucleus.
Vitolo et al. (1992)IL2lymphocytesIn contrast, tumor-infiltrating lymphocytes (TIL) in the stroma of ovarian carcinomas or most ductal breast tumors only rarely expressed mRNA for TNF alpha, IL2 or IFN gamma.
Vitolo et al. (1992)IL-2mononuclear cellThe intensity of mononuclear cell infiltration in these tumors correlated positively with the percentage of cells which expressed mRNA for IL-2, TNF alpha and IL-2R.
Chou et al. (2009)IL-2PBMCData from reverse transcription-polymerase chain reaction (RT-PCR) and real-time PCR indicated that IL-2 and IFN-gamma mRNA expression in PBMC could be induced by 2-OH.
Kroemer et al. (1991)IL-2T lymphocytesIn the peripheral lymphoid organs, IL-2 is produced by a defined population of mature CD4+ T lymphocytes in which the IL-2 gene is transcribed or silenced, depending on the combination of antigenic and nonspecific activation signals to which the cell is exposed.
Paliogianni et al. (1993)interleukin 2 geneProstaglandin E2 and other cyclic AMP elevating agents inhibit interleukin 2 gene transcription by counteracting calcineurin-dependent pathways.
Paliogianni et al. (1993)IL-2 geneT cellWe have previously shown that prostaglandin E2 and other cAMP elevating agents inhibit the nuclear transcription of the human IL-2 gene by interfering with a Ca(2+)-sensitive T cell signal transduction pathway.
Paliogianni et al. (1993)IL-2 geneWe have therefore tested the hypothesis that this phosphatase may be a target for the inhibitory effects of cAMP on IL-2 gene transcription.
Tanaka and Tsujimura (2002)IL-2lymphocytesCyclosporin is a non-cytotoxic immunomodulating drug which inhibits NF-AT-dependent IL-2 transcription in lymphocytes.
Fujino et al. (1997)IL-2Furthermore, pretreatment with Oxo-M enhanced PHA-induced mRNA expression of the alpha and beta subunits of IL-2 receptors and DNA synthesis.
Arya and Gallo (1984)TCGF geneNuclear transcription experiments suggested that the TCGF gene was more actively transcribed in cells treated with TPA and phytohemagglutinin than in cells treated with phytohemagglutinin alone.
Arya and Gallo (1984)TCGF geneThe transcription of the TCGF gene was further increased when interleukin 1 was included along with TPA and phytohemagglutinin.
Depper et al. (1985)IL-2Interleukin 2 (IL-2) augments transcription of the IL-2 receptor gene.
Depper et al. (1985)IL-2IL-2 stimulation results in increased accumulation of IL-2 receptor mRNA within 4 hr, while an increase in IL-2 receptor gene transcription is detected within 30 min in isolated nuclei.
Waldmann (1986)IL-2In HTLV-I-infected cells, the 42-kDa long open reading frame (tat) protein encoded in part by the tat region of HTLV-I may act as a transacting activator that induces transcription of the IL-2-receptor gene, thus providing an explanation for the constant association of HTLV-I infection of lymphoid cells and IL-2-receptor expression.
Garrity et al. (1994)Interleukin-2Interleukin-2 transcription is regulated in vivo at the level of coordinated binding of both constitutive and regulated factors.
Garrity et al. (1994)Interleukin-2T cellsInterleukin-2 (IL-2) transcription is developmentally restricted to T cells and physiologically dependent on specific stimuli such as antigen recognition.
Garrity et al. (1994)IL-2T cellsWe conclude that binding activities of all classes fail to stably occupy their cognate sites in IL-2, except following activation of T cells, and that specificity of IL-2 transcription is enforced at the level of chromosomal occupancy, which appears to be an all-or-nothing phenomenon.
Belldegrun et al. (1990)IL-2macrophageIL-2-expanded 444P.3 cells, tested on day 104 in culture, expressed mRNA for tumor necrosis factor (TNF), IL-2 and tumor growth factor beta (TGF-beta) but not for IL-1, lymphotoxin or granulocyte/macrophage-colony stimulating factor (GM-CSF).
Belldegrun et al. (1990)IL-2The parental noncloned population expressed mRNA for TNF, lymphotoxin, GM-CSF and TGF-beta but not for IL-1 beta or IL-2.
Bahbouhi and al-Harthi (2004)IL-2Analysis of interleukin 2 (IL-2) mRNA expression using real-time reverse transcription PCR (RT-PCR) of the HIV-enriched population demonstrated a higher level of IL-2 mRNA, by approximately four cycles, and an 8-fold increase in IL-2 expression, as evaluated by intracellular staining and flow cytometric analysis, in comparison to gp41-negative cells.
Raab et al. (2001)interleukin 2In this study, we show that CD28 can cooperate with VAV/SLP-76 adaptors to upregulate interleukin 2/4 transcription independently of TCR ligation.
Lafont et al. (1998)interleukin-2T cellsIt has been shown that stimulation of lymphoid cells causes the activation of the extracellular signal-regulated-2 (ERK-2) which activates nuclear factor of activated T cells (NF-AT), a transcription factor involved in the regulation of interleukin-2 (1L2) gene transcription.