Viewing negative mentions of transcription of IL2: interleukin 2 (Homo sapiens)

Full-text article links are indicated by after the article reference.

Document Target Regulator Anatomy Sentence
Benjamin et al. (1989)IL-2T cellThe following similarities in the functional biological characteristics of T cell and B cell IL-2 suggest that B cell IL-2 is not a factor which mimics IL-2 activity in the CTLL-2 assay: (i) neutralization of IL-2 by anti-IL-2 monoclonal antibody (DMS-1); (ii) elution of IL-2 following its adsorption to CTLL-2 cells; (iii) determination of the MW of IL-2 by SDS-PAGE and Western blot analysis; and (iv) ability of B cell IL-2 to support T cell proliferation and blocking of this activity by anti-tac monoclonal antibody. cDNA probes for T cell IL-2, however, did not detect IL-2 mRNA in B cells.
Burton et al. (1994)IL-2Furthermore, uninduced HuT-102 cells did not express mRNA encoding IL-2 as assessed by Northern blot analysis.
Gulino et al. (1990)IL-2 mRNAHeLaStable transformants (NIH-3T3,HeLa and BFS cells) carried an intact transfected IL-2 gene and constitutively expressed cytoplasmic human IL-2 mRNA which was not detected in vector-transfected cells.
Goebels et al. (1988)IL-2 mRNAT cellIn these cell lines, IL-2 mRNA was not detectable in RNA extracted from whole adult T cell leukemia cell populations because of dilution by other RNA species from the vast majority of cells that do not contain IL-2 mRNA.
Lopez et al. (1998)IL-2Expression of IL-2 activity was not detected; however, mRNA encoding IL-2 was produced and translatable in vitro.
Conti et al. (1999)IL-2 proteinliverRESULTS: IL-2 protein and its mRNA were absent in the liver, with minimal expression of IL-2 receptor, during rejection.
Leonard et al. (1985)IL-2Alternative messenger RNA (mRNA) splicing may delete exon 4 sequences, resulting in a mRNA that does not encode a functional IL-2 receptor.
Noma et al. (1989)IL-2lymphocytesPatients' lymphocytes whose IL-2 responsiveness was decreased still expressed Tac antigen (low-affinity IL-2 receptors) but, in contrast to the patients' original lymphocytes, did not absorb or respond to IL-2, suggesting the loss of high-affinity IL-2 receptors (p55/p75) from these cells.
Boussiotis et al. (1997)interleukin-2T cellsIn the absence of costimulation, T cells activated through their antigen receptor become unresponsive (anergic) and do not transcribe the gene encoding interleukin-2 (IL-2) when restimulated with antigen.
Kaufmann et al. (1985)IL-2Moreover, IL-2 receptors were not detected on the hybrid cells before and after antigenic stimulation, demonstrating that expression of IL-2 receptors and induction of specific killing activity are not genetically linked.
Isakov et al. (1985)IL 2-specificPBLFurthermore, IL 2-specific mRNA was not detected in TCD-stimulated PBL, demonstrating that IL 2 was not required for TCD-induced T cell proliferation.
Milstone and Parker (1986)TCGFThe 14300-Mr protein was labelled within 5 min after addition of [35S]methionine and was the predominant intracellular form of TCGF, whereas the 16300-Mr protein was not detected until 25 min later and was the predominant extracellular form of TCGF.
Lüscher et al. (1994)IL-2TILA primary tumor cell line was generated and cultured TIL were induced to transcribe IL-2 and IFN-gamma genes by incubation with the autologous irradiated tumor cell line, but not with autologous EBV-transformed cells.
Eckels et al. (1999)IL-2Lack of IL-2 induction was confirmed by a failure to amplify IL-2 mRNA upon NS3 antigen stimulation, whereas IL-4, IL-15, and gammaIfn mRNA were seen as early as 24 h.
Foon et al. (1985)IL-2The highest secreting clone was demonstrated to have mRNA to IL-2 while the parent CCRF-H-SB2 had no detectable mRNA to IL-2.
June et al. (1987)IL-2 mRNACyclosporine was found not to affect the accumulation of IL-2 mRNA after CD28 plus PMA stimulation, although there was no detectable IL-2 mRNA after stimulation by CD3 in the presence of the drug.
Schaller et al. (1990)IL2lymphocytesAfter 2-3 weeks under immunosuppressive treatment with prednisolone and azathioprine, however, BP lymphocytes did not exhibit any IL2 receptors.
Grant et al. (1996)IL-2More than 90 days after transplantation, IL-2 mRNA was detected more frequently with mild or absent rejection (10% in grades 0 and 1 vs. 0% in grades 2 and 3, P=0.078).
Osawa and Diamantstein (1989)IL-2T lymphoblastsFurther, we showed that the AHT-107 did not dissociate IL-2 from the high-affinity IL-2R complex once formed on human T lymphoblasts.
Bosque et al. (2007)IL-2T cellIn the absence of IL-2, human CD4(+) T cell blasts were sensitive to both FasL and Apo2L/TRAIL, but human CD8(+) T cell blasts died, with no additional effect of death receptor ligation.
Linardopoulos et al. (1992)IL-2There was no transcription of the interleukin-2 (IL-2) or the interleukin-2 receptor (IL-2R) genes and Tac expression was not seen.
Umadome et al. (1988)IL-2Although the expression of IL-2 or IL-4 mRNA was not detected in fresh leukemic cells, the expression of IL-2 mRNA, but not IL-4 mRNA, was induced by phytohemagglutinin stimulation, and the leukemic cells proliferated.
Bechara et al. (2008)IL-2fatBoth in treated and non-treated fat tissue, mRNA of IFN-gamma, IL-2, and IL-5 was not expressed.
Fan et al. (1993)IL-2 mRNAT cellsElevated levels of IFN-gamma mRNA and lowered IL-2 mRNA were found in the PBMC of eight seropositive men with CD4 T cells over 500/mm3 (mean, 647/mm3), whereas IL-4 and IL-10 mRNA were not changed significantly.
Flescher et al. (1995)IL-2 mRNAAlthough we detected no IL-2 mRNA in ALD-treated cells, the nuclei of these cells contain proteins capable of binding to three regulatory sequences in the IL-2 promoter region: NFAT, NF kappa B, and AP-1.
Salmon et al. (1989)IL-2One clone failed to express IL-4 mRNA, but did produce those for IL-2 and IFN-gamma.
Schellens et al. (2008)IL-2Furthermore, no correlation was observed between IL-2 and/or IFN?
Leonard et al. (1985)IL-2T cellsResting T cells do not contain detectable IL-2 receptor mRNA.
Ben Aribia et al. (1989)IL-2NK cellsTac mRNA transcripts accumulated rapidly in NK cells during a 18-h observation period whether anti-Tac antibody was present or not during IL-2 stimulation.
Jokhi et al. (1994)IL-2T cellsIn contrast, peripheral blood CD56brightCD16- NK cells, CD56dimCD16+ NK cells, and CD3+ T cells expressed mRNA only for TNF-alpha and TGF-beta 1, but not for GM-CSF, CSF-1, IFN-gamma, LIF, or IL-2.
Schierano et al. (2003)IL-2Transcripts for IL-2, IFN-gamma, IL-12, and IL-4 were absent.
Carding et al. (1989)lymphokineThymocytesThymocytes at 14 days of gestation and after 16 days up until birth do not express detectable lymphokine mRNA.
Li et al. (2007)IL-2However, IL-2 was not detected in non-responders after the booster (P < 0.01).
Vandekerckhove et al. (1994)IL-2IL-2, perforin, and IFN-gamma mRNAs were almost absent in CD4+CD8+ CD3low but were clearly detectable in CD4+CD8+ CD3high cells, indicating that these genes are induced at the CD3low- CD3high transitional stage.
Venetsanakos et al. (1997)IL-2IL-2, IL-4 and tumour necrosis factor (TNF)-alpha mRNA was not detected in any of the normal breast tissues.
Hellquist et al. (1996)IL-2NK cellsWe found evidence for the homing of T cells, and for generation of NK cells in the vicinity of the tumours. mRNA for IL-2 and IL-12, were identified but IL-4 mRNA was not found.
Proust et al. (1991)IL 2Northern blot analysis confirmed the accumulation of p55 specific mRNA and the absence of the IL 2 transcript.
Gigout et al. (1998)IL2IL2, IL4 and IFN gamma mRNAs were either undetectable or weakly detectable throughout the study, with no major changes.
Green et al. (1997)IL-2Transcripts for IL-2, IL-3, IL-5, IL-7 and IFN-gamma were not detected in either group.
Srivastava et al. (1996)IL-2Maternal cells in breast milk expressed mRNA for MCP-1 (20/20), IL-8 (14/20), TGF-beta 1 (14/16), TGF-beta 2 (4/6), M-CSF (9/12), IL-6 (6/12) and IL-1 beta (7/12), and may be a source of these cytokines. mRNA for IL-2, IL-10, IFN-gamma, TNF-alpha was not detected and only weak expression was found for RANTES (1/18).
Fujihashi et al. (1996)IL-2In most samples, mRNA for IL-2, IL-4 and IL-5 were not detected by cytokine-specific RT-PCR.
Boehme et al. (1995)IL-2accessory cellsT cells that had been activated and induced to proliferate with IL-2 were found to express high levels of IL-2 mRNA upon TCR rechallenge, without a requirement for accessory cells.
Legoux et al. (1992)IL-2At the same time we found that transcripts for IL-2, IL-3, IL-4, IL-5, IFN gamma, huMlP1 alpha and huMlP1 beta were totally absent in this cell line.
Jensen et al. (1998)IL-2IL-2 production was triggered by the specific interaction between the CD20 molecule and the scFvFc:zeta as IL-2 was not detected in cultures with mock transfected Jurkat cells or CD20- stimulator cells.
Syme et al. (1997)IL-2The requirement for CD4 cells was complex, since CD8 enriched cells failed to express mRNA for IL-2, suggesting that CD4-dependent IL-2 production was required for CD8-cell proliferation.
Howell et al. (1991)IL-2IL-2 mRNA was found in the absence of IL-2 protein in RA biopsies (six surgical, 12 needle), but variable amounts of IL-2 protein were detected in six out of seven needle biopsies from SpA, PsA and JCA patients, where CD3+ lymphoid infiltrates were present.
Xu et al. (2008)IL-2Interleukin-6 (IL-6) or IL-10 was induced in some acellular graft-implanted monkeys at the early time points, but tumor necrosis factor-alpha (TNF-alpha), interferon-gamma (IFN-gamma), or IL-2 was not detected over the study period.
Boyle-Walsh et al. (1996)IL-2Transcripts for IL-1 alpha, IL-2, IL-4, IL-5, IL-7, TNF alpha, or IFN gamma were not detected in any of the meningioma cultures.
Caplen et al. (1992)IL-2 geneT cellOur results demonstrated that transcripts for DR, DP, and DQ could be detected in cells which were neither dividing nor transcribing mRNA for another marker of T cell activation, the IL-2 gene.
Vitolo et al. (1992)IL2LNLIn contrast, LNL not adjacent to the tumor in involved LN, as well as those in tumor-uninvolved LN, did not express mRNA for cytokines or IL2 receptor.
Golstein et al. (1986)interleukin 2Further investigations of SC functions indicated that no interleukin 2 (IL-2) biological activity was detected in culture supernatants of SC constimulated with PHA and phorbol myristate acetate (PMA).
Christoforidou et al. (2004)IL-2No circulating IL-2 was detected in the sera of the patients or the control donors (data not shown).
Benjamin et al. (1989)IL-2B cellThe following similarities in the functional biological characteristics of T cell and B cell IL-2 suggest that B cell IL-2 is not a factor which mimics IL-2 activity in the CTLL-2 assay: (i) neutralization of IL-2 by anti-IL-2 monoclonal antibody (DMS-1); (ii) elution of IL-2 following its adsorption to CTLL-2 cells; (iii) determination of the MW of IL-2 by SDS-PAGE and Western blot analysis; and (iv) ability of B cell IL-2 to support T cell proliferation and blocking of this activity by anti-tac monoclonal antibody. cDNA probes for T cell IL-2, however, did not detect IL-2 mRNA in B cells.
King et al. (1995)IL-2IFN-gamma, IL-2 and LIF mRNA were not found, although all samples contained LIF-receptor mRNA.
Zeitz et al. (1988)IL-2LPLThis finding was confirmed at the molecular level as IL-2 mRNA was not detected in unstimulated LPL but was found in concanavalin A-stimulated LPL.
Gulino et al. (1990)IL-2 mRNANIH-3T3Stable transformants (NIH-3T3,HeLa and BFS cells) carried an intact transfected IL-2 gene and constitutively expressed cytoplasmic human IL-2 mRNA which was not detected in vector-transfected cells.
Pérez et al. (2001)IL-2vesiclesThe presence of gamma interferon IFN-gamma and interleukin-2 (IL-2) mRNAs but absence of IL-4, IL-5, and IL-10 mRNAs were observed in peripheral blood mononuclear cells from immunized subjects after in vitro challenge with outer membrane vesicles.
Nakagomi et al. (1995)IL-2Expression of IL-2, IL-3 and IL-4 mRNA was not detected in any of the tissues analyzed.
Baan et al. (1994)IL-2In contrast, IL-2 and IL-4 gene transcripts were absent in these samples.
Hardin et al. (1990)IL-2Furthermore, three hybridomas which produced BHF did not transcribe detectable levels of mRNAs specific for IL-1, IL-2, IL-3, IL-4, IL-5, IL-6, GM-CSF, or IFN-gamma.
Hildesheim et al. (2002)IL-2Linearity of results was observed when dilutions of a single specimen were blindly tested, with the exception of IL-2 and IL-10, where deviations from linearity were sometimes observed.
Bill et al. (1994)IL-2lymphocyteThese effects on IL-2 mRNA were not general because IL-2 receptor mRNA stability was not changed even though it also is transiently expressed during the course of lymphocyte activation.
Falk et al. (1989)IL-2Production of IL-2 or IL-2 mRNA was not detected under these conditions.
Atayar et al. (2006)IL2T cellsIn contrast to the CD4(+)/CD57(+) T cells from tonsils, IL2 and IL4 mRNAs were consistently absent from the CD4(+)/CD57(+) T cells of NLPHL.
Bansal et al. (2009)IL-2Other cytokines (such as IL-2, IL-4 and IL-10) were not detected in the supernatant of the colonic explants cultured in the presence or absence of parasites.
De Boer et al. (1992)IL-2IL-2 was not detected after the first BCG instillation, but from the second instillation onwards the mean IL-2 concentration increased rapidly.
van der Meer et al. (1988)interleukin 2macrophageThis RIA does not detect human lymphotoxin, interleukin-1 alpha or beta, interleukin 2, interleukin 6, interferon alpha or gamma, granulocyte-macrophage-colony stimulating factor, and C5a des arg.
Melby et al. (1994)IL-2IL-2 and IL-5 mRNAs were not found.
Strehlau et al. (2002)Interleukin-2RESULTS: Interleukin-2 (IL-2) and IL-4 transcripts were not observed in any of the specimens.
Moudgil et al. (1997)IL-2Therefore, we studied cytokine gene expression in auto- and allo-MLR by Northern dot blotting and reverse transcriptase-polymerase chain reaction (RT-PCR) analysis. mRNA for IL-beta and IL-8 was detected in both auto- and allo-MLR by Northern dot blotting. mRNA for IL-2, gamma-IFN, TNF-alpha, IL-4, IL-10 and IL-2 receptor (IL-2R) was not found by Northern dot blotting and could only be detected by RT-PCR.
Utsugisawa et al. (1994)IL-2On the other hand, we could not find both IL-1 beta mRNA and IL-2 mRNA in three normal thymuses.
Kumaki et al. (1998)IL-2In contrast, IL-2 was not detected at any time in these patients.
Kutukculer et al. (1998)IL-2PlasmaPlasma IL-2 and IL-4 levels were not elevated and were found to be similar to those in the inactive phase and in healthy controls.
Berger et al. (1995)IL-2IL-2 and IL-5 mRNA were also detected in 2 of the patients but not in the other samples.
Wang et al. (1995)IL-2T cellsIL-2 mRNA was not detected, despite the presence of T cells as defined by the expression of CD3 gamma mRNA.
Rosenbaum et al. (1995)IL-2T-cellRESULTS: Using Wilcoxon rank sum analysis, mRNA for interleukin (IL)-1 alpha or IL-8 was significantly more abundant in corneas from patients with pseudophakic bullous keratopathy relative to either comparison group. mRNA for the T-cell marker, CD4, and for T-cell derived cytokines, IL-2, IL-4, and interferon gamma, could not be detected in any corneal sample.
Zambello et al. (1997)IL-2Using RT-PCR analysis, we demonstrated that highly purified CD3+ and CD3- GL did not express mRNA for IL-15 or IL-2.
Bonay et al. (1997)IL-2T cellsOnly IFN-gamma mRNA expression was detected in all individuals evaluated, whereas IL-2 mRNA was not detected during the first 48 h of stimulation, and T cells from only one of three venom-allergic individuals produced detectable IL-4 or IL-5 mRNA.
Yamaoka et al. (1995)IL-2RESULTS: Although IL-1 beta and IFN-gamma mRNA were detected in most specimens, IL-2, IL-3, IL-4, IL-5, IL-9, TNF-alpha, and IFN-beta mRNA were not detected at all.
Bluth et al. (2003)IL-2PBMCOn day 0, when IgG and IgE anti-parvovirus were detected in serum, patient's peripheral blood mononuclear cells (PBMC) expressed mRNA for the Th2 cytokines IL-4 and IL-10, but not for the Th1 cytokines IFN-gamma or IL-2.
Odukoya et al. (1997)IL-2Ovarian IL-2 and IL-4 mRNA were not expressed in either group.
Corrigan et al. (1995)IL-2T-LCComparing the asthmatics with the controls, elevated percentages of CD4 T-LC expressed mRNA encoding IL-5, IL-4, and GM-CSF (P < 0.02) but not IL-3, IL-2, or IFN-gamma.
Nakajima et al. (1996)IL-2The mRNA for IL-2, IL-5, or IFN-gamma was not detected.
McCubrey et al. (1990)LymphokineLymphokine gene expression, which would support growth, was not detected in FD/PMA lines.
Christensen et al. (1998)IL-2Likewise, IL-2 mRNA was not found in any of the BC-PCs.
Ohgama (1993)IL-2T cellBy contrast, among the CD4+ T cell subpopulations no difference in the Con A response was observed and only slightly higher IL-2 secretion was detected in gp90MEL-14 negative CD4+ T cells than in gp90MEL-14 positive cells.
Yamamoto et al. (1997)IL-2Except for a few cases, messages for IL-2, IL-4 and IL-5 were not detected by cytokine-specific RT-PCR.