Viewing affirmative mentions of protein catabolism of IL2: interleukin 2 (Homo sapiens)

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Duprez et al. (1988)IL2T cellWe have described a human tumor T cell line, IARC 301, which constitutively expresses high affinity interleukin 2 (IL2) receptors, and showed that after binding to its receptors, IL2 is endocytosed and degraded.
Fleischmann et al. (1990)IL-2IL-2-IN activity is susceptible to bacterial and endogenous urinary proteolysis.
Shaw et al. (1988)IL-2 mRNAThe decline occurs despite a high rate of synthesis, and appears to be due to an enhanced rate of IL-2 mRNA degradation.
Shaw et al. (1988)IL-2 mRNAThe degradation of IL-2 mRNA is selectively sensitive to cycloheximide and actinomycin D, inhibitors of protein and RNA synthesis, respectively.
Fujii et al. (1986)interleukin 2T cellsHigh-affinity receptor-mediated internalization and degradation of interleukin 2 in human T cells.
Fujii et al. (1986)IL-2T cellReceptor-mediated internalization and degradation of IL-2 were investigated in cell lines carrying human T cell leukemia/lymphoma (lymphotrophic) virus type I (HTLV-I) and PHA-treated normal PBL.
Fujii et al. (1986)IL-2The internalized IL-2 was rapidly degraded and the products were excreted into the culture fluid.
Fujii et al. (1986)IL-2The t1/2 of IL-2 degradation in these cells was estimated as 60-80 min at 37 degrees C.
Fujii et al. (1986)IL-2The internalization and degradation of IL-2 were both temperature dependent.
Ohmura and Onoue (1990)interleukin 2lymphocytesThe rate of the degradation of interleukin 2 (IL-2) mRNA produced in stimulated human tonsillar lymphocytes was found to be significantly decreased in cells continuously stimulated with a calcium ionophore, A23187, and a phorbol ester, phorbol 12, 13-dibutylate (PDB) as compared with that in unstimulated cells.
Ohmura and Onoue (1990)IL-2 mRNAIL-2 mRNA produced was rapidly degraded when the stimulants were washed out at 12 h and the cells further cultured in the presence of actinomycin D, which stops mRNA synthesis.
Ohmura and Onoue (1990)IL-2 mRNAlymphocytesFurther study demonstrated that the rate of degradation of 32P-labeled IL-2 mRNA, which was prepared by cell-free transcription of IL-2 cDNA, in the polysomal fraction obtained from PDB-stimulated lymphocytes was decreased compared with that obtained from unstimulated lymphocytes.
Legrue et al. (1991)IL-2These observations support the hypothesis that IL-2/IL-2R endocytosis and ligand/receptor processing or degradation may be important steps in lymphokine signal transduction.
Robb et al. (1981)TCGFIn addition, at 37 degrees C, a receptor- and lysosome-dependent degradation of TCGF occurred.
Wang et al. (2008)IL-2During storage, both glycosylated and unglycosylated IL-2 mutein form aggregates (soluble and insoluble) and other degradation products.
Tanaka et al. (1988)IL-2The acid-wash technique revealed that more than 60% of 125I-labeled IL-2 bound to the cells became acid-unremovable in the first 30 min of incubation at 37 degrees C and degradation products of 125I-IL-2 increased after 30 min of incubation.
Legrčs et al. (1995)IL-2The degradation of uncomplexed 125I-rhIL-2 has also been observed in the presence of trypsin, whereas 125I-rhIL-2 bound to alpha 2M-MA is protected.
Ariel et al. (1998)IL-2neutrophilIn contrast, neutrophil elastase, an enzyme activated during inflammation, degrades IL-2 to yield IL-2 fractions that inhibit IL-2-induced T cell adhesion to FN.
Subtil et al. (1994)IL2plasmaAfter ligand binding to receptors on the plasma membrane, receptor-ligand complexes are rapidly endocytosed and IL2 is degraded in acidic compartments.
Ferrer et al. (1993)IL2lymphocytesWhen these two chains are associated in lymphocytes, they form high-affinity receptors that mediate IL2 endocytosis and degradation, and transduce IL2 signaling.
Yoshimura and Kahan (1985)IL-2lymphocytesTherefore the present study examined the effect of CsA administered in vivo on the capacity of kidney transplant recipient lymphocytes to generate IL-2 after mitogen (phytohemagglutinin [PHA]) stimulation.
Benjamin et al. (1989)IL-2T cellThe following similarities in the functional biological characteristics of T cell and B cell IL-2 suggest that B cell IL-2 is not a factor which mimics IL-2 activity in the CTLL-2 assay: (i) neutralization of IL-2 by anti-IL-2 monoclonal antibody (DMS-1); (ii) elution of IL-2 following its adsorption to CTLL-2 cells; (iii) determination of the MW of IL-2 by SDS-PAGE and Western blot analysis; and (iv) ability of B cell IL-2 to support T cell proliferation and blocking of this activity by anti-tac monoclonal antibody. cDNA probes for T cell IL-2, however, did not detect IL-2 mRNA in B cells.
Noma et al. (1984)IL2Bestatin therefore increases both IL2 sensitivity and IL2 production.
Kolosov et al. (1992)interleukin-2In the course of translation of mRNA the interleukin-2 becomes aggregated due to the adsorption of this protein onto the ribonucleoprotein complex.
Yamada et al. (1995)interleukin-2[Wilson-Jones type angiosarcoma with marked response to intrapleural administration of interleukin-2].
D’Alessandria et al. (2009)99mTc-HYNIC-IL2Stability of 99mTc-HYNIC-IL2
Soiffer et al. (1994)IL-2We have previously shown that IL-2 administered to patients at very low doses after BMT is both clinically tolerable and immunologically active.
Corbo et al. (1987)IL2All the four IL2-secreting clones integrated in the mouse genome the human IL2 gene.
Yen et al. (2000)IL-2Involvement of the ubiquitin-proteasome pathway in the degradation of nontyrosine kinase-type cytokine receptors of IL-9, IL-2, and erythropoietin.
Liang et al. (1989)IL-2In addition, the degradation of IL-2 in the cells was enhanced by GSH treatment.
Fallon et al. (2000)IL-2We demonstrate here that the internalization kinetics of 2D1 via the high affinity IL-2 receptor are equivalent to those of WT but that a significantly increased fraction of internalized 2D1 is sorted to recycling instead of to lysosomal degradation.
Borth and Teodorescu (1986)IL-2Soybean trypsin inhibitor coupled to Sepharose 4B was capable of absorbing the IL-2 degrading activity from the trypsin solution.
Podtschaske et al. (2007)IL-2Control steps in activation-induced IL-2 expression and relevant for this switch decision influenced by ionomycin or CsA could be (i) the IL-2 mRNA formation and its degradation, (ii) IL-2 promoter accessibility for the initiation of IL-2 mRNA transcription, (iii) cooperative and synergistic interaction between the transcription factors NFAT, NF-?
Farag et al. (2002)IL-2PURPOSE: The purpose of the study is to investigate the tolerability of interleukin 2 (IL-2) after intensive chemotherapy in elderly acute myeloid leukemia (AML) patients in first complete remission (CR).
Nakamura et al. (1990)interleukin-2pleural cavity[Eosinophil colony stimulating activity induced by administration of interleukin-2 into the pleural cavity of patients with malignant pleurisy].
Wu et al. (1986)IL-2In addition, there was no evidence of an age-related change in IL-2 mRNA degradation or the post-transcriptional processing of IL-2 mRNA.
Itescu et al. (1997)IL-2Following treatment with TNFa at 1 u/ml, IL-2 activated human NK lysis of PAEC was inhibited at every effector:target (E:T) ratio tested.
Makedonas et al. (2010)IL-2, IL-2, and degranulation.
Ohnishi et al. (1989)IL-2kidneyTo confirm this notion, 125I-IL-2 was incubated in vitro with kidney homogenate, which degraded 125I-IL-2 in acidic pH.
Ohnishi et al. (1989)IL-2kidneyAdditional experiments using a heat-treated kidney cytosol fraction plus cathepsin D, and pepstatin inhibition on the degradation of 125I-IL-2 by cathepsin D, a major acid protease in the kidney, resulted in the identification of this enzyme to be responsible for the degradation of 125I-IL-2.
Ohnishi et al. (1989)IL-2kidneyOverall, these results demonstrated that the kidney is the organ to metabolize IL-2 and that cathepsin D, a renal acid protease, is involved in the degradation of IL-2.
Miedel et al. (1989)interleukin-2Limited proteolysis of a recombinant, soluble form of the Tac protein, a human interleukin-2 receptor (rIL-2R), was performed using trypsin, Staphylococcus aureus V8 protease and proteinase K to study the structural requirements of interleukin-2 receptor (IL-2R) for interleukin-2 (IL-2) binding.
Ke et al. (2002)IL-2It is concluded that after MLR, B 7 increased IL-2 secretion by decreasing the degradation of IL-2 mRNA and upregulating IL-2 transcription factors.
Cha et al. (1997)IL-2Since both GFP and IL-2 were rapidly degraded by protease activity during the fourth day post-infection (another disadvantage with larvae), we found an accurate determination of harvest time was critical.
Kenney et al. (1986)IL-2Parameters for the evaluation of IL-2 stability.
Kenney et al. (1986)interleukin-2Recombinant DNA derived interleukin-2 stability in accelerated and long term studies was evaluated using biological and protein chemical methods.
Cadée et al. (2002)rhIL-2In contrast with non-degradable dex-MA hydrogels, degradable dex-lactate-HEMA gels with comparable network characteristics (degree of methacrylate substitution and initial water content) showed an almost zero-order, degradation controlled release of rhIL-2 in a time period of 5-15 days.
Roifman et al. (1985)IL-2Comparative studies are necessary to demonstrate the efficacy, species specificity, and stability of recombinant IL-2.
Safar and Junghans (2000)IL2The manufacturer's data indicate stability of the IL2 for a period of 6 days.
D’Alessandria et al. (2009)IL2Due to the limited stability of IL2 at pH?
D’Alessandria et al. (2009)99mTc-HYNIC-IL2molarThe stability of 99mTc-HYNIC-IL2 was also evaluated in the presence of increasing amounts of DTPA, with molar ratios ranging from 1:1 to 1000:1 DTPA:99mTc-HYNIC-IL2.
Hopkins and Failla (1999)IL-2 mRNAIL-2 mRNA stability in parental Jurkat cells was independent of Cu status.
Smith (2006)IL2-bound IL2 being stabilized by binding to isolated ?
Ohmura and Onoue (1990)IL-2 mRNAT lymphocytesStability of IL-2 mRNA in T lymphocytes is controlled by a protein kinase C-regulated mechanism.
Ohmura and Onoue (1990)IL-2 mRNAHowever, the stability of IL-2 mRNA was increased by the addition of PDB or A23187.
Ohmura and Onoue (1990)IL-2 mRNAThe effect of PDB was dose-dependent and inhibited by the inhibitors of protein kinase C (PKC), staurosporine, and K252a, suggesting the involvement of PKC in the control of IL-2 mRNA stability.
Ohmura and Onoue (1990)IL-2 mRNAThe involvement of protein phosphorylation in the regulating mechanism of IL-2 mRNA stability was supported by the fact that the addition of okadaic acid, which inhibits serine/threonine protein phosphatases, resulted in an increase in the stability of IL-2 mRNA.
Ohmura and Onoue (1990)IL-2 mRNAThese results indicate the presence of a mechanism controlling the stability of IL-2 mRNA that is regulated by PKC.
Duprez et al. (1994)interleukin 2T lymphocyteThe T lymphocyte growth factor interleukin 2 binds to surface high-affinity receptors and is rapidly internalized and degraded in acidic organelles.
Lowenthal et al. (1986)IL2These results provide ultrastructural information on the intracellular pathway of IL2 and on its probable site of degradation within the cell.
Hua et al. (1993)IL2 mRNAThe mRNase cleaves IL2 mRNA at a small number of sites in the coding region, and IL2 mRNA containing only the coding region and 36 nucleotides of the 3'-noncoding region competes efficiently with full-length IL2 mRNA for the mRNase, whereas beta-globin mRNA does not.
Petersen et al. (1987)interleukin-2B-lymphocytesBinding, uptake and degradation of human recombinant interleukin-2 (125 ala) in activated human T- and B-lymphocytes and in monocyte-macrophages.
Petersen et al. (1987)IL-2monocyteIt is concluded that high-affinity receptors mediate binding, uptake and degradation of IL-2 in activated human T- and B-lymphocytes and in monocyte-macrophages.
Hájek et al. (2003)IL-2stem cellsTreatment of chronic myeloid leukemia with autologous transplantation using peripheral blood stem cells or bone marrow cultured in IL-2 followed by IL-2, GM-CSF, and IFN-alpha administration.
Hua and Paetkau (1996)interleukin 2 mRNAPreparations of the binding factor also protect interleukin 2 mRNA against degradation by a recently described RNasin-resistant endoribonuclease activity in Jurkat cells.
Wang et al. (2008)IL-2The thermal stability of IL-2 mutein was studied by DSC and UV.
Wang et al. (2008)IL-2SAIn summary, we found that the dual effects of Tween 80 on the stability of IL-2SA, such as that shaking-induced aggregation of IL-2 mutein was significantly inhibited in the presence of Tween 80.
Wang et al. (2008)IL-2However, this surfactant adversely affected the stability of IL-2 mutein in solution during storage in terms of both oxidation and aggregation.
Tanaka et al. (1988)IL-2Novel IL-2-binding molecules (p70 and p75) mediating internalization and degradation of IL-2 were examined by employing a human B lymphoblastoid line, SKW6-4 cells.
Theander et al. (1988)interleukin-2cleavageInhibition of human lymphocyte proliferation and cleavage of interleukin-2 by Pseudomonas aeruginosa proteases.
Theander et al. (1988)IL-2lymphocyteThese results indicated that P. aeruginosa-derived enzymes are able to interfere with human lymphocyte function in vitro and that this effect might be due to cleavage of IL-2.
Shackelford and Trowbridge (1986)interleukin-2The human interleukin-2 (IL-2) receptor was quantitatively cleaved into two large disulfide-bonded fragments by either trypsin or endoproteinase lys-C (endo lys-C).
Reyes et al. (2005)interleukin-2Stability of Ala 125 recombinant human interleukin-2 in solution.
de Oca et al. (2010)IL-2T cellsHere, we demonstrate that IL-2Rbeta is cleaved in cell lines of different origin, including T cells, generating an intracellular 37-kDa fragment (37beta ic) that comprises the full intracellular C-terminal and transmembrane domains.
Tzannis et al. (1996)interleukin 2We studied both the bioactivity and physical stability of interleukin 2 upon delivery via continuous infusion.
Klimka et al. (2003)interleukin-2Construction of proteolysis resistant human interleukin-2 by fusion to its protective single chain antibody.
Baig et al. (2005)IL-2IL-2 degradation was completely inhibited by 1,10 phenanthroline and partially inhibited by bestatin, suggesting that a metallo-aminopeptidase was responsible.
Mintz et al. (1993)IL-2T-cellThe ability of the protease to degrade IL-2 and cleave CD4 on human T cells suggests that protease may contribute to the pathogenesis of Legionnaires' disease by impeding T-cell activation and immune function.
Kojiro et al. (1989)recombinant interleukin-2[Intrapleural administration of recombinant interleukin-2 in a patient with pleural effusion due to non-Hodgkin's lymphoma].
Henics et al. (1994)lymphokineThese findings suggest a direct correlation between differential binding of cytoplasmic proteins to AU-rich 3'-UTRs in vitro and lymphokine mRNA stability in vivo.
Liu et al. (2005)MvIL-2superiorHowever, the stability of MvIL-2 was superior than that of IL-2 at different temperatures.
D’Alessandria et al. (2009)99mTc-HYNIC-IL2The stability of 99mTc-HYNIC-IL2 was evaluated by incubating 100 µl of 99mTc-HYNIC-IL2 in 900 µl of either human serum or saline for 24 h at 37°C.
D’Alessandria et al. (2009)99mTc-HYNIC-IL2Fig. 5Analysis of 99mTc-HYNIC-IL2 stability in human serum, saline, and DTPA solution after purification by tC2 Sep-Pak.
Yu and Malek (2001)IL-2tailProteasome function was also necessary for the lysosomal degradation of IL-2 internalized by IL-2R that were comprised of cytoplasmic tailless beta- or gamma c-subunits, suggesting that the target protein for the proteasome is independent of either the cytoplasmic tail of the IL-2R beta- or gamma c-subunits and their associated signaling components.
Yu and Malek (2001)IL-2Therefore, a functional proteasome is required for optimal endocytosis of the IL-2R/ligand complex and is essential for the subsequent lysosomal degradation of IL-2, possibly by regulating trafficking to the lysosome.
Henics (1999)interleukin 2Our findings may explain some aspects of differential regulation of interleukin 2 (IL-2) and TNFalpha mRNA stability upon microfilament disruption in human PBMCs observed in an earlier study.
Miedel et al. (1989)interleukin-2Limited proteolysis of recombinant human soluble interleukin-2 receptor.
Ansorge and Schön (1987)lymphokineThese data, together with other features of the DP IV, support the notion that this enzyme plays a key role in the modulation of lymphokine action by X-Pro- or X-Ala-directed limited proteolysis.
Calvo (1994)IL-2In contrast, a posttranscriptional alteration of the IL-2 mRNA is shown, by demonstrating that the degradation rate of IL-2 mRNA following the addition of actinomycin D, at 4 h, was delayed in the (PHA+PMA)-activated cell cultures containing 10(-12) M of SP.
Yu and Malek (2001)interleukin-2In the present study, we investigated the role of the proteasome for ligand-dependent endocytosis and degradation of the interleukin-2 (IL-2)-interleukin-2 receptor (IL-2R) complex.
Kuo et al. (1998)IL-2A sequence-similarity search in the GenBank database indicates that the major sequence of 1.7-kb L1 is integrated in human retinoblastoma (Rb), IL-2, and factor VIII genes.
Robb and Greene (1987)IL-2The kinetics of IL-2 internalization mediated by the intermediate-affinity beta chain were nearly identical to those of the high-affinity alpha/beta heterodimer (t1/2 of 10-15 min), and each type of receptor targeted the bound IL-2 for intracellular degradation in lysosomes.
Ricci et al. (2003)interleukin-2granulocytepH Dependence of structural stability of interleukin-2 and granulocyte colony-stimulating factor.
Nagy et al. (1994)IL-2 mRNAT cellThese studies also show that IFN-gamma and IL-2 mRNA stability are temporally dissociated during the course of T cell activation, and we propose that this dissociation may be mediated through the production of IL-12.