Viewing affirmative mentions of localization of IL2: interleukin 2 (Homo sapiens)

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Bödeker et al. (1981)IL-2Optimal IL-2 release was found at a low speed of stirring allowing circulation of the medium without suspending the agglutinated cells, and at an oxygen concentration of about 30-45% of saturation.
Foa et al. (1992)IL2The constitutive secretion of IL2 by the tumour cells leads to a reduced or abrogated tumorigenicity in several different tumour models.
Martin et al. (1989)IL-2T cellNone of the generated T cell hybridomas exhibited antigen-specific IL-2 secretion when stimulated with autologous thyrocytes, although 60% of the hybridomas expressed CD3 antigen and the T cell receptor alpha/beta heterodimer.
De la Hera et al. (1987)IL2mature T cellsMitogenic membrane ligands have been shown to activate interleukin 2 (IL2) production only in mature T cells, IL2 constitutive secretion having not yet been demonstrated.
De la Hera et al. (1987)IL2thymocytesInterestingly, their proliferation in the absence of any exogenous stimulating agent was related to an autonomous use of the IL2 system (IL2 secretion and binding to its specific receptor, whose constitutive, functional expression in human early thymocytes was recently shown).
Kaplan et al. (1988)IL-2T cellSurface IL-2 epitopes were also detected on the Jurkat tumor cell line which secretes IL-2 upon stimulation and on another T cell tumor line MOLT 4.
Kaplan et al. (1988)IL-2Thus, cells with the potential of producing and secreting IL-2 upon stimulation possessed the surface epitopes of the lymphokine and cells either actively secreting IL-2 or without the potential for secretion were negative for surface expression.
Kaplan et al. (1988)IL-2T cellsAlthough it is possible that the epitopes seen were present on a distinct molecule independent of secreted IL-2, the distribution on a variety of T cells and regulation via cellular activation suggest that the surface expression of IL-2 epitopes is in some way related to the soluble lymphokine.
Conradt et al. (1988)IL-2lymphocytesHuman peripheral blood lymphocytes secrete high titers of interleukin-2 (IL-2) after stimulation by Ca2+-ionophore A23187/phorbol 12-myristate-13-acetate.
Conradt et al. (1988)IL-2During the first 30 hours of incubation cells secrete only the nonglycosylated IL-2 M form of the lymphokine, the glycosylated forms IL-2 N1,2 being detected only after prolonged culture times (30-48 h).
Conradt et al. (1988)IL-2After recultivation of cells for a second 48 h period (without additional mitogen), the glycosylated and nonglycosylated IL-2 forms are secreted at a constant ratio of 7:3 throughout.
Meuer et al. (1982)IL 2T cellMitogen stimulation led to secretion of equivalent amounts of IL 2 from both the major T cell subsets; in contrast, after allogeneic activation, IL 2 was produced predominantly from the T4+ subset.
Sirota et al. (1995)IL-2colostrumAt concentrations as low as 0.5%, human colostrum stimulated IL-2 production; at a higher concentration (10%), IL-2 secretion was inhibited.
Uchiyama et al. (1993)IL-2IL-2 gene was transfected into three human melanoma cell lines; secretion of IL-2 from stable transfected cells was confirmed by enzyme-linked immunosorbent assay.
Kalashnikova and Pukhal'ski? (1993)interleukin-2lymphocytesDose-dependent modifying of mitogen-induced interleukin-2 (IL-2) release by human peripheral blood lymphocytes (PBL) treated with dexamethasone (Dx) was demonstrated.
Kalashnikova and Pukhal'ski? (1993)IL-2PBLIn four independent experiments with PBL of 10 human subjects the effect of Dx administration on IL-2 release in serum-free culture medium could vary from appreciable enhancing to complete suppression.
Kalashnikova and Pukhal'ski? (1993)IL-2Mechanisms of IL-2 release modification by Dx are discussed.
Vose (1987)IL-2Helper T cellsHelper T cells can be induced to proliferate upon exposure to cells of the autologous tumour and to secrete detectable levels of interleukin 2 (IL-2).
Reem et al. (1984)IL-2thymocytesStimulation with con A in combination with PMA or Bl cells resulted in secretion of IL-2 by dense, immature thymocytes, low-density, mature thymocytes, and unfractionated thymocytes, and in an increase in [3H]thymidine incorporation.
Faist et al. (1993)IL-2mononuclear cellThe major regulatory level of interleukin-2 (IL-2) release under stressful conditions was determined via parallel analysis of IL-2 messenger RNA (mRNA) expression and IL-2 protein synthesis in mitogen-stimulated peripheral blood mononuclear cell (PBMCs) cultures on consecutive days postinjury.
Marijani et al. (2007)IL-2The bioactivity of ciclosporin MDI formulations was evaluated by determining the ciclosporin-mediated inhibition of interleukin-2 (IL-2) release from human Jurkat cells stimulated with phorbol 12-myristate 13-acetate (PMA).
Stopeck et al. (1998)IL-2IL-2 secretion by tumor cells persisted for at least 30 days post-transfection and was unaffected by freeze thawing or irradiation to 8000 rads.
Stopeck et al. (1998)IL-2Enzyme-linked immunoabsorbent analysis of the amount of IL-2 secreted into the medium by transfected tumor cells correlated with the percentage of tumor cells expressing intracellular IL-2 as measured by flow cytometry.
Belli et al. (1997)interleukin-2From January 1994 to July 1996 we immunized metastatic melanoma patients with HLA-A2-compatible, interleukin-2 (IL-2)-secreting, immunogenic melanoma lines in an attempt to induce a systemic reaction that might also affect distant melanoma lesions.
Belli et al. (1997)IL-2These results indicate that vaccination with cells bearing the appropriate antigens and releasing IL-2 locally can produce weak clinical responses, but also indicate that better results may be achieved through modifications of the vaccine, the schedule of immunization and/or a more appropriate selection of patients.
Paradisi et al. (1995)IL-2leukocyteThese findings confirm IL-2 to be in seminal plasma, show increased IL-2 secretion in the infertile group, demonstrate negative correlations of IL-2 levels with main spermiogram parameters, and indicate no correlation with leukocyte count.
Solbach et al. (1982)Il-2T cellIn order to release Il-2, the OKT4 positive T cell requires a stimulus, such as allogeneic cells or the lectin phytohaemagglutinin A (PHA).
Vie and Miller (1986)IL 2T cellsEstimation by limiting dilution analysis of human IL 2-secreting T cells: detection of IL 2 produced by single lymphokine-secreting T cells.
Gulino et al. (1990)IL-2HeLaConstitutive expression of IL-2 mRNA in human IL-2 gene-transfected NIH-3T3 and HeLa cells was associated to the secretion of bioactive IL-2 protein, while no IL-2 production was observed in untransfected or vector-transfected cells.
Niu et al. (1996)IL2In order to investigate the antitumor effect of IL2 gene transfected tumor vaccine, gene transfected tumor cells which secrete IL2 in a comparatively sustained manner must be obtained.
Niu et al. (1996)IL2IL2 secretion of B16-IL2 was comparatively stable for a period of 6 months with the highest production rate of 53u/ml.
Clouse et al. (1984)TCGFmononuclear cellsSecretion of TCGF is induced after stimulation of human peripheral blood mononuclear cells ( PBMNC ) with herpes simplex virus type 1 (HSV-1).
Sasagawa et al. (2006)IL-2Simultaneous measurement of secreted IL-2 by ELISA and cell viability by the XTT assay showed that the 95:5 ethanol/water extract of E. purpurea was both IL-2 suppressive and cytotoxic at 50 and 100 microg/mL.
Castignolles et al. (1994)interleukin-2Human recombinant interleukin-2 can be associated and released from supramolecular biovectors (SMBVs), consisting of particles made of polymerized polysaccharides.
Viviani et al. (1992)IL-2Alterations of both IL-2 and melatonin secretion have been reported in cancer patients.
Froelich et al. (1988)interleukin-2lymphocytesPhytohemagglutinin induced proliferation by aged lymphocytes: reduced expression of high affinity interleukin-2 receptors and interleukin-2 secretion.
Froelich et al. (1988)interleukin-2Cultures from two groups of aged donors, recruited respectively from our ambulatory clinic and a nursing home, incorporated less tritiated thymidine (3H-TdR) and secreted less interleukin-2 than did young donors.
Froelich et al. (1988)IL-2It was also shown that the amounts of soluble IL-2 receptors that were released into the supernatants by mitogen stimulated cells did not differ for the aged and young donors.
Pinkston et al. (1987)interleukin 2T lymphocytesCorticosteroid therapy suppresses spontaneous interleukin 2 release and spontaneous proliferation of lung T lymphocytes of patients with active pulmonary sarcoidosis.
Pinkston et al. (1987)IL 2helper T lymphocytesActive pulmonary sarcoidosis is characterized by the alveolar accumulation of activated helper T lymphocytes that are spontaneously releasing IL 2 and proliferating at an enhanced rate.
Pinkston et al. (1987)IL 2lungOver 3.2 +/- 0.4 mo, the untreated group had no significant change in spontaneous lung T cell release of IL 2 or spontaneous proliferation.
Pinkston et al. (1987)IL 2T cellIn contrast, over the same period, the treated group had marked reduction of spontaneous lung T cell release of IL 2 and proliferation (p less than 0.01, all comparisons before therapy).
Uchino et al. (2002)IL-2IL-2 release from the cells was enhanced by SQOOH and increased at a non-cytotoxic dose.
Burkitt and Aggarwal (2000)IL-2macrophagesAfter "poly-plat" treatment macrophages developed cytoplasmic extensions much faster and secreted higher levels of interleukin-2 (IL-2), compared to CDDP.
Burkitt and Aggarwal (2000)IL-2Poly"Poly-plat" treated human fibroblasts demonstrated a significant release of IL-2 (138 pg/ml), compared to CDDP (64 pg/ml) or the untreated normal cells (31 pg/ml) after 8 h.
Duc Dodon and Gazzolo (1987)IL2T cellsThe results also suggest that viral attachment to T cells possibly supplies an accessory function triggering autocrine secretion of IL2 by these cells.
Cadée et al. (2002)interleukin-2In this study, the release of recombinant human interleukin-2 (rhIL-2) from methacrylated dextran (dex-MA) and (lactate-)hydroxyethyl methacrylated dextran (dex-(lactate-)HEMA) hydrogels with varying crosslink density was investigated.
Cadée et al. (2002)rhIL-2The protein release profiles both the non-degradable and degradable dextran-based hydrogels showed that with increasing crosslink density of the gel, the release of rhIL-2 decreases.
Cadée et al. (2002)rhIL-2From dex-MA hydrogels with an initial water content above 70%, the rhIL-2 release followed Fickian diffusion, whereas from gels with an initial water content of 70% or lower the protein was fully entrapped in the hydrogel meshes.
Cadée et al. (2002)rhIL-2In contrast with non-degradable dex-MA hydrogels, degradable dex-lactate-HEMA gels with comparable network characteristics (degree of methacrylate substitution and initial water content) showed an almost zero-order, degradation controlled release of rhIL-2 in a time period of 5-15 days.
Cadée et al. (2002)rhIL-2Importantly, rhIL-2 was mainly released as monomer from the hydrogels and with good retention of its biological activity.
Mingari et al. (1984)interleukin 2B cellCytolytic clones were further analyzed for their ability to release interleukin 2 (IL2) and B cell growth factor(s) (BCGF) upon 24 h stimulation with phytohemagglutinin.
Dautry-Varsat et al. (1988)interleukin-2T-cellThe effect of cyclosporin A (CsA), a potent immunosuppressive agent, on a human T-cell line, IARC 301, which constitutively secretes interleukin-2 (IL-2) and expresses high-affinity IL-2 receptors, was investigated.
Dautry-Varsat et al. (1988)IL-2T-cellCsA also prevents the constitutive secretion of IL-2 in this T-cell line by blocking transcription of the IL-2 gene.
Goebel et al. (2002)IL-2lymphocyteIn the next week, re-exposure to the conditioned stimulus (drink), but now paired with placebo capsules, induced a suppression of immune functions as analyzed by the IL-2 and IFN-gamma mRNA expression, intracellular production, and in vitro release of IL-2 and IFN-gamma, as well as lymphocyte proliferation.
Borysiewicz et al. (1985)IL-2cytotoxic T cellThe immunosuppressive effects of measles virus on T cell function--failure to affect IL-2 release or cytotoxic T cell activity in vitro.
Borysiewicz et al. (1985)IL-2lymphocytesSimilarly, IL-2 release was not affected by Herpes simplex virus infection of such cultures, although lymphocytes infected with Sendai or respiratory syncytial viruses produced considerably less IL-2.
Wee and Bach (1984)Interleukin-2T cellVarious functionally distinct human T cell clones derived from in vitro mixed leukocyte cultures are found to secrete lymphokines with detectable Interleukin-2 (IL-2)-like activity upon antigenic stimulation.
Weiss et al. (1984)IL 2T cellThese studies demonstrated that antibodies reactive with the T cell-specific T3 antigen were insufficient to result in the activation of Jurkat cells, determined by the secretion of IL 2.
Voss et al. (1989)IL-2lymphocytesWe have used an enzyme-linked immunosorbent assay (ELISA) to quantify the role of released and cell-bound IL-2 receptor following in vitro or in vivo activation of human lymphocytes with IL-2.
Voss et al. (1989)IL-2lymphocytesIn vitro experiments, culturing fresh peripheral blood lymphocytes in 30 U/ml IL-2 (corresponding to the steady-state IL-2 concentration achieved in patients receiving IL-2 in our clinical trials), showed that the levels of IL-2 receptor released into the culture media exceeded the levels of cell-associated receptor, with both rising in parallel to the cytotoxic activity of the peripheral blood lymphocytes (PBL) against cultured tumor cells.
Voss et al. (1989)IL-2In 12 patients receiving high-dose IL-2 for the treatment of various malignant neoplasms, the levels of IL-2 receptor released into the serum rose dramatically during the IL-2 infusion, and then fell following cessation of the IL-2 infusion.
Voss et al. (1989)IL-2This heightened release of IL-2 receptor into the serum occurred during the episodes of profound lymphopenia that developed within hours after patients began an IL-2 infusion.
Ruscetti and Gallo (1981)TCGFT cellsThus, release of TCGF and development of receptors for it appear to be obligatory for the clonal expansion of all activated T cells.
Cech et al. (2006)IL-2T cellsEchinacea alkylamides suppressed IL-2 secretion by stimulated T cells, and this effect was significantly lessened upon oxidation of the alkylamides to carboxylic acids and hydroxylated metabolites.
Orvieto et al. (1997)interleukin-2embryosThe study was conducted to determine whether pre-implantation embryos secrete interleukin-2.
Orvieto et al. (1997)interleukin-2large granular lymphocytesIt may therefore be speculated that interleukin-2 secreted by human embryos may modulate the function and replication of large granular lymphocytes.
Lindqvist et al. (1987)interleukin-2PBLWe show in this study that phorbol esters alone can induce peripheral blood mononuclear cells (PBL) to secrete interleukin-2 (IL-2) and that the IL-2-dependent cell line CTLL can be used for measuring this lymphokine without influence of the phorbol esters themselves.
Lindqvist et al. (1987)IL-2A significant positive correlation between blockage of induced cell aggregation by antibody 60.3 and reduction in IL-2 release was observed.
Lindqvist et al. (1987)IL-2The addition of interleukin-1 (IL-1) restored IL-2 secretion in PHA-treated, but not in 4-beta-phorbol 12, 13-dibutyrate [P(Bu)2]-treated, cells in the presence of this antibody.
Aoki et al. (1997)IL-2AECNormal human AEC secrete IL-2 at rest (7 pg/ml), and IL-2 secretion is increased threefold after stimulation with PMA + histamine; this increase is inhibited by dexamethasone and diphenhydramine.
Hunninghake et al. (1983)IL-2T-cellsThese studies suggest that the release of IL-2 by lung T-cells may explain in part the local proliferation of T-cells and hypergammaglobulinemia that are characteristic of pulmonary sarcoidosis.
Manger et al. (1986)interleukin 2T cellDifferent T cell lines, which can be induced to secrete interleukin 2 (IL-2) in vitro, were used to dissect the effect of cyclosporin A (CsA).
Cignetti et al. (1994)IL2These findings have prompted investigations into whether human tumor cells may be transduced with the IL2 gene and whether tumor cell lines could be engineered to release IL2.
Cignetti et al. (1994)IL2Messenger RNA expression of the IL2 gene was analyzed by reverse transcriptase-polymerase chain reaction (RT-PCR) and productive IL2 release using a human IL2 assay and an enzyme-linked immunosorbent assay kit.
Cignetti et al. (1994)IL2The amounts of IL2 constitutively released by the engineered neoplastic cells ranged between 1 and 11 U/mL of IL2 produced from 10(6) cells in 72 hours.
Cignetti et al. (1994)IL2In nude mice injected with various IL2-producing ST4 clones, tumor growth associated inversely with the amounts of IL2 secreted by the leukemic cells.
Johnson et al. (1992)interleukin-2T-cellsConditioned media from interleukin-2 secreting Jurkat T-cells as well as the glucocorticoid-insensitive, but receptor positive clone, CEM-C1, failed to prevent lymphocytolysis; exogenous interleukin-2 also did not provide protection.
Hathaway and Adams (2004)Interleukin-2bloodMETHODS: Interleukin-2 secretion by blood mononuclear cells was measured in response to recall antigens, alloantigen (donor and third-party), and phytohemagglutinin.
Babkin et al. (2008)IL-2The authors show the capacity of CV-1 cells infected with the recombinant vaccinia virus VV-SIL2 to secrete human IL-2 into the culture medium.
Westendorp et al. (1994)interleukin-2T cellsHuman immunodeficiency virus type 1 Tat upregulates interleukin-2 secretion in activated T cells.
Westendorp et al. (1994)IL-2T cellsIL-2 secretion was increased more than twofold in both Jurkat T cells and primary T cells stimulated by extracellular HIV-1 Tat protein.
Liu and Rosenberg (2001)IL-2Furthermore, an Ab reactive with the alpha-chain of IL-2R complex reduced the viability mediated by IL-2 secretion of the IL-2 transductants.
Vives et al. (1987)IL-2thymocytesCD3-4-8- and unfractionated thymocytes were compared for their capacity to proliferate, to express interleukin 2 (IL-2) receptor, and to secret IL-2.
Vives et al. (1987)IL-2A similar level of IL-2 secretion in both populations was also obtained when they were stimulated with a combination of phorbol ester plus ionophore.