Viewing negative mentions of localization of IL2: interleukin 2 (Homo sapiens)

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Lamb et al. (1983)IL-2The clones that functioned as helper cells in the production of specific antibody failed to release detectable amounts of IL-2.
Vaith et al. (1985)interleukin-2lymphocytesThere was no measurable interleukin-2 (IL-2) secretion of peripheral blood lymphocytes.
Efrat and Kaempfer (1984)IL-2oocytesThe growth response of murine cells shows that IL-2 secreted from human cells has properties not found in the IL-2 secreted from oocytes, even though a monoclonal antibody directed against the human IL-2 receptor blocks the activity of both types of IL-2.
Ythier et al. (1985)IL2In fact, there was no measurable interleukin 2 (IL2) secretion and no significant increase in IL2 susceptibility following incubation with either PHA or anti-T11(2) plus anti-T11(3).
Bogunia-Kubik et al. (2003)IL-2T cellsStimulation of T cells in the presence of CB sera increased the frequency of IL-2 producing cells (p < 0.005) (but not the amount of IL-2 secreted) and resulted in a higher expression of CD25 (p < 0.05).
Bergmann et al. (1991)interleukin 2Since recombinant interleukin 2 is not secreted by bacteria, but is instead obtained from bacterial lysates, we have analysed several different recombinant interleukin 2 molecules as well as the naturally synthesized cytokine by phase separation in Triton X-114.
Katzen et al. (1985)IL 2monocytesIn contrast to T cells stimulated with PHA in the presence of monocytes, T cells stimulated with PHA and IL 2 released no detectable IL 2.
Skerka et al. (1995)IL-2In unstimulated cells which do not secrete IL-2, only Sp1 binds to this region, while in stimulated IL-2 secreting cells the inducible EGR-1 protein recognizes this element.
Inaba and Steinman (1984)interleukin 2B cellLyt-2-T blasts did not grow or release interleukin 2 or B cell helper factors unless rechallenged with specific alloantigen, whereupon growth (generation time of 14-18 h) and lymphokine release rapidly resumed.
Shapiro et al. (1990)IL-2CTLL-2IL-2-independent CTLL-2 clones did not secrete detectable quantities of IL-2 into culture supernatant and exhibited reduced sensitivity to the inhibitory effects of both IL-2 and IL-2 receptor antibody.
Arima et al. (1987)IL 2Group 2 (seven patients): Cells of this group did not secrete IL 2 when cultured in mitogen-free medium, but the cells showed response to exogeneously added recombinant IL 2 and proliferated in culture.
Arima et al. (1987)IL 2Group 4 (three patients): Cells of this group did not secrete IL 2 in mitogen-free medium.
Walker et al. (1986)IL 2Addition of exogenous IL 2 did not release cells arrested in the G1a phase.
Alfonzo et al. (2003)interleukin-2CTLUntreated patients displayed CMV-specific cytotoxic T-lymphocyte (CTL) activity despite the absence of CMV-specific lymphoproliferative responses (LPRs) and despite the fact that interferon-gamma (IFN-gamma) and interleukin-2 (IL-2) were not secreted.
Danzer et al. (1994)IL-2Among more than 100 random MLC experiments, we observed one cytokine pattern similar to the cytokine release detected in a control MLC of HLA-identical siblings, which showed marginal or no secretion of IL-2, sIL-2R, IFN-gamma, TNF-alpha, and IL-6.
Huland et al. (1997)IL-2Thus, patients who could not tolerate high-dose systemic IL-2 were able to tolerate inhalation IL-2 therapy.
Huland et al. (1999)IL-2Response of patients treated with inhalation of interleukin-2 (IL-2), which can be offered to those not suitable for systemic therapy, was compared to risk factors.
Nieto et al. (1990)IL-2Nevertheless, IL-2 is not secreted by these activated cells.
Schwartzentruber et al. (1988)IL-2Two patients were subsequently retreated with IL-2 without complication.
Kaufmann et al. (1984)IL 2We have found that a pharmacologic level of CsA (10 ng/ml) blocks antigen- and lectin-driven interleukin 2 (IL 2) secretion without affecting cell proliferation.
Tavel et al. (2010)IL-2METHODOLOGY: Participants not on continuous ART with > or = 300 CD4(+) cells/mm(3) were randomized to: no treatment; IL-2 for 5 consecutive days every 8 weeks for 3 cycles; or the same IL-2 regimen with 10 days of ART administered around each IL-2 cycle.
Pinkston et al. (1983)interleukin-2T cellsBlood T cells from the same patients did not release interleukin-2.
Raad et al. (2001)interleukin-2bloodCONCLUSION: In patients at low risk for infection from infusion- or catheter-related infection who are not receiving total parenteral nutrition, blood transfusions, or interleukin-2, delaying the replacement of IV tubing up to 7 days may be safe, as well as cost-effective
Inaba et al. (1985)interleukin 2dendritic cellsThe aggregates that form with syngeneic dendritic cells disassemble within hours and do not release interleukin 2 or proliferate.
Voss et al. (1989)IL-2lymphocytesFollowing cessation of the IL-2 infusion these activated lymphocytes return to the peripheral circulation and do not secrete TAC as vigorously as while influenced directly by the IL-2 infusion.
Zicari et al. (1995)IL-2Tissues did not release IL-2.
Elkahwaji et al. (1999)IL-2Hepatic CYPs and monooxygenase activities were not significantly decreased in 5 patients receiving daily doses of 3 or 6 10(6) IL-2 units/m2, compared to 7 patients who did not receive IL-2.
Umetsu et al. (1988)IL-2T cellWe identified human T cell clones which secrete IL-4 but not IL-2 or IFN-gamma, and which appeared to correspond to murine Th2 clones.
Matsushita et al. (1994)IL-2 mRNANorthern blot analysis did not show any IL-2 mRNA.
Yamada et al. (1991)IL-2T cellIt also revealed through the use of an in vitro assay utilizing the human IL-2 dependent cell line, Sez 627, that none of these T cell lines secrete IL-2 in detectable volumes.
Ndhlovu et al. (2010)IL-2T cellsThat said, the failure of IL-2 to expand IL-17 producing CD4+ T cells while increasing T-reg populations may augur well for IL-2 use in auto-immunity, diseases characterized by depleted T-reg populations, and elevated IL-17 expression.
Liu et al. (1991)IL-2The L3T4+ cells secreted IL-2 and had characteristics of T helper cells, while the 38+ cells did not secrete IL-2 and appeared to be T cytotoxic-suppressor cells.
Giovarelli et al. (1988)IL-2lfIn seven further cases (25.9%), small quantities of IL-2lf (0.2-1.7 U) were detected, while only in four (14.8%) no release was found.
Blasco et al. (1995)IL-2T cellCD3- Jurkat cell variants failed to secrete IL-2, suggesting the involvement of the T cell receptor/CD3 complex pathway in jacalin signaling.
Foa et al. (1985)IL 2T lymphocytesThe spontaneous release of IL 2 and IFN-gamma was practically absent both with B-CLL and normal T lymphocytes.
Mingari et al. (1991)IL-2In addition, following stimulation with phytohemagglutinin (PHA) and phorbol 12-myristate 13-acetate (or PHA alone) all clones released interferon-gamma and tumour necrosis factor-alpha, but not IL-2.
Marrack et al. (1983)interleukin 2It was found that subclones of the hybridoma that no longer secreted interleukin 2 in response to Ag/H-2, even though they continued to secrete interleukin 2 in response to concanavalin A, also no longer bound specifically to Ag-pulsed monolayers of the appropriate H-2 type.
Tennenberg and Weller (1996)IL-2T cellIL-2 was not secreted as part of the LPS-induced T cell activation response.
Charley et al. (1985)porcine IL2A neutralizing polyclonal anti-human IL2 antiserum was also unable to neutralize porcine IL2.
Resta et al. (1994)IL-2CD73 transfectants of Jurkat mutants defective in the TCR, in p56lck, or in the tyrosine phosphatase CD45 did not secrete IL-2 in response to CD73 mAb + PMA, suggesting a role for the CD3/TCR-signaling complex in CD73-mediated signal transduction.
Long et al. (2001)IL-2HuT 78A PKCbeta-deficient clone of HuT 78, K-4, did not secrete IL-2 in response to PMA stimulation.
Podtschaske et al. (2007)IL-2The isolated IL-2 secreting cells had a 25-fold greater IL-2 mRNA-content than the cells not secreting IL-2 (Fig. 2), indicating that the expression of IL-2 in the activated Th cells is regulated upstream of transcription.
Anderson and Teuber (2010)IL-2However, immunomodulatory effects are present, including an inhibition of cellular proliferation despite no decrease in IL-4 or IL-2.
Bagnasco et al. (1988)IL-2Finally, 44% of intrathyroid clones were neither cytolytic nor able to release IL-2 and gamma-interferon.
Matsushima et al. (1985)interleukin 2thymocyteSix of eight human EBV-B cell lines spontaneously produced and released detectable levels of thymocyte comitogenic factor in vitro, but no interleukin 2 (IL-2) activity.
Paliard et al. (1988)IL-2T cellTwo alloantigen-specific and two tetanus toxoid-specific T cell clones secreted IL-2, IL-4, and IFN-gamma simultaneously, whereas one alloantigen-specific T cell clone secreted IL-2 and IFN-gamma, but not IL-4.
Hamann et al. (1997)IL-2T cellsThese T cells do not secrete IL-2 or -4 but can produce IFN-gamma and TNF-alpha.
Cayeux et al. (1989)IL-2T cellsIn contrast, when normal T cells were cultured with non-T cells derived from patients found between 20 and 330 days after ABMT, no IL-2 secretion and no proliferative responses could be seen.
Krusemeier and Snow (1988)interleukin-2T cellsThe stimulation of CSA-treated T cells with specific antigen in the presence of low concentrations of CSA, demonstrated that the T cells did not release detectable levels of interleukin-2, interleukin-4, and interleukin-5.
Grabbe et al. (1994)IL-2IL-8 was secreted at a high level throughout the culture, whereas no spontaneous secretion of IL-2, IL-3, IL-4, and IL-7 was measured at all.
Sturmh÷fel et al. (1995)IL-2Signaling through the zeta-chain was required, as cells transfected with a chimera containing only a 22 amino-acid long, truncated zeta-chain did not secrete IL-2 constitutively.
Del Prete (1992)IL-2Human type 1 helper (Th1), but not type 2 helper (Th2), cells produce interleukin-2 (IL-2), gamma-interferon (IFN-gamma), and tumor necrosis factor-beta, whereas Th2, but not Th1, cells secrete IL-4 and IL-5, but not IL-2 or IFN-gamma.
Moiola et al. (1994)IL-2Th1 cellsMost lines secreted IL-2 and not IL-4, suggesting that they comprise Th1 cells.
Van Reijsen et al. (1994)IL-2Upon stimulation with allergen or anti-CD3+ phorbol myristate acetate (PMA) IL-4 was released with or without IL-5, while no (or extremely low concentrations of) IL-2 and interferon-gamma (IFN-gamma) were detectable.
Emara and Carroll (1992)recombinant IL-2PBLsHowever, crosslinking CD7 and/or FcRmu of PBLs significantly reduced phytohemagglutinin (PHA)-induced IL-2 production and rendered PBLs unable to utilize exogenously added human recombinant IL-2 (rIL-2).
Sell et al. (1992)interleukin-2T-cellAnalysis of cytokines secreted by these individual clones showed that the antiidiotype-reactive cloned T-cell lines that suppressed the response of idiotype-bearing cells appear to secrete predominantly interferon gamma, whereas those antiidiotype-reactive cloned T-cell lines that augmented the response do not secrete interferon gamma but secrete interleukin-2, -4, and -6.
Bosticardo et al. (2001)IL-2T lymphocytesT lymphocytes stimulated with anti-CD3 mAb or PHA at low pH(e) were unable to secrete IL-2 and IFN-gamma and their ability to progress through the cell cycle was impaired.
Takahashi et al. (1995)IL-2T cellsThese CD4+ T cells released IFN-gamma, IL-4, and TNF-alpha, but not IL-2, in response to HLA-DR15+ target cells.
Barcy et al. (1995)IL-2T cellWhen cultures were prepared in 96-well plates coated with human IgG, stimulation of T cells with allogeneic monocytes resulted only in modest T cell proliferation and no detectable IL-2 secretion as compared with untreated culture plates or plates coated with Fab fragments of human IgG.
Geppert et al. (1992)IL-2T cellsCD4+ T cells did not secrete IL-2 when cultured alone, with control or CD8+ CHO cells.
Swain et al. (1990)IL-2Those cells that do survive continue to secrete IL-4 and IL-5 but not IL-2.
Lollini et al. (1997)IL-2IL-15 is typically produced by normal cells that do not release IL-2, but little information is currently available on human tumors.
Sivanandham and Mukherji (1989)IL-2PBLOne of these two lines, referred to as TFTx, established from the autologous tumor activated peripheral blood lymphocytes (PBL), grows in culture without any exogenous interleukin-2 (IL-2), secretes no detectable amount of IL-2 or gamma interferon (IFN) or tumor necrosis factor (TNF) alpha or beta.
Pantaleo et al. (1988)IL-2All of the five clones selected for detailed analysis did not express CD4, CD8, and CD28 antigens and did not release IL-2, whereas they displayed cytolytic activity against NK-sensitive, NK-resistant, and fresh tumor target cells.
Swain et al. (1990)IL-2Our studies show that the presence of IL-4 during the response of naive Th cells causes precursors to develop into a population comprised largely of "Th2-like" effectors that secrete IL-4 and IL-5, but little IL-2 or IFN-gamma.
Salvadori et al. (1995)IL-2spleenWe found that neither B7-1+ nor IL-2-secreting, B7-1+ CMS5 cells stimulated naive spleen cells to proliferate or to become cytotoxic.
Harari et al. (2004)IL-2T cellsHowever, a skewing toward IFN-gamma-secreting cells (70% of HIV-1-specific CD4 T cells) was observed in subjects with progressive disease, and IL-2- and IL-2/IFN-gamma-secreting cells were almost absent.
Nagy et al. (2009)IL-2Na´ve PBMCs, which did not respond to IL-2, did not display binding to either probe, thus verifying that constitutive NF?
Lowenthal et al. (1986)IL 2EL4EL4-6.1 cells do not constitutively secrete IL 2, nor do they express IL 2-R; but when cultured in the presence of recombinant IL 1, they secrete detectable amounts of IL 2 (5 to 15 U/ml).
Ramirez et al. (1987)B cell growth factorT cellsSLE serum or Ig fractions did not induce B cell growth factor release by T cells.
Lauritzsen and Bogen (1991)IL2Six of the clones are of the Th1 type as they secrete IL2 and IFN gamma, but not IL4.
Tsubata et al. (2005)IL-2C did not grow in the absence of IL-2, and were close to cell death approximately 2 days after IL-2 withdrawal.
Moiola et al. (1994)IL-2They secreted IL-2, not IL-4, suggesting that they comprised T helper type 1 cells.
Shimizu et al. (1980)TCGF-ETCGF-E is neither synthesized or secreted by this lymphoma cell line unless stimulated by Con A.
Katzen et al. (1985)IL 2T cellsIn contrast to T cells stimulated with PHA in the presence of monocytes, T cells stimulated with PHA and IL 2 released no detectable IL 2.
Del Prete et al. (1991)IL-2In contrast, most TES-specific TCC secreted IL-4 and IL-5 but not, or limited amounts of, IL-2 and IFN-gamma.
Del Prete et al. (1991)IL-2PPD-specific TCC that failed to secrete IL-4 and IL-5, and TES-specific TCC that failed to secrete IL-2 and IFN-gamma, were found to lack transcripts for IL-4 and IL-5, or for IL-2 and IFN-gamma, respectively.
Vukmanovi? et al. (1992)IL-2T-cellsWe have identified a patient whose peripheral T-cells failed to proliferate and secrete IL-2 upon stimulation.
Manigold et al. (2010)IL-2Remarkably, Gn(465-473)-specific cells readily secreted IFN-gamma, granzyme B and TNF-alpha but not IL-2 upon stimulation and showed a 'revertant' CD45RA(+)CD27(-)CD28(-)CCR7(-)CD127(-) effector memory phenotype, thereby resembling a phenotype seen in other latent virus infections.
Del Prete et al. (1991)IL-2Under both these experimental conditions, the great majority of PPD-specific TCC secreted IL-2 and IFN-gamma but not, or limited amounts of, IL-4 and IL-5.
Auphan-Anezin et al. (2003)IL-2T cellIndeed, we characterized a T cell population stimulated in response to the partial agonist that was committed to produce IFN-gamma, but failed to divide or secrete IL-2.
Pelosi-Testa et al. (1988)IL-2PBMInterestingly, lectin-pulsed PBM do not release detectable amounts of IL-2 in the medium.