Viewing affirmative mentions of phosphorylation of IL2: interleukin 2 (Homo sapiens)

Full-text article links are indicated by after the article reference.

Document Target Regulator Anatomy Sentence
Zu et al. (1990)interleukin 2We have characterized the cellular proteins which are rapidly phosphorylated by interleukin 2 (IL 2) in a human IL 2 dependent cell line.
Kung et al. (1989)interleukin-2Phosphorylation of human interleukin-2 (IL-2).
Kung et al. (1989)IL-2Recombinant human IL-2, expressed in either E. coli or cos cells, was shown to be phosphorylated by protein kinase C.
Kung et al. (1989)IL-2T cellThere was no difference in biological activity between non-phosphorylated and phosphorylated IL-2, as determined by a T cell growth assay.
Kung et al. (1989)IL-2Although the physiological role of phosphorylation of IL-2 is unclear, IL-2 can be labeled with [gamma-32P] ATP and protein kinase C to a high specific radioactivity, and the synthesis of biologically active 32p-labeled IL-2 may be useful for receptor-binding studies of the cells containing low level of phosphoprotein phosphotases.
Mills et al. (1990)IL2Similar concentrations of suramin were required to inhibit binding of 125I-labeled IL2, IL2-induced tyrosine phosphorylation, and IL2-induced proliferation, suggesting that these processes may be linked.
Asao et al. (1990)IL-2Rp75T cellsThe present study using TU11 mAb demonstrates the IL-2-induced phosphorylation of IL-2Rp75 on tyrosine residues in IL-2-dependent T cells.
Asao et al. (1990)IL-2Rp75Phosphorylation of serine and threonine residues of IL-2Rp75 is also detected, but its IL-2 dependency is not significant during at least the first 5 min.
Mills et al. (1990)Interleukin 2Interleukin 2 receptor beta is tyrosine phosphorylated.
Sabe et al. (1984)IL-2HeLaPhorbol ester-induced phosphorylation of the IL-2 receptors on HeLa cells did not affect the affinity of the receptor.
Evans et al. (1992)Interleukin-2T-cellsInterleukin-2-dependent phosphorylation of the retinoblastoma-susceptibility-gene product p110-115RB in human T-cells.
Goebel et al. (2000)IL-2Since this is the molecular weight of the IL-2R beta- and gamma-chains, which are both tyrosine-phosphorylated by IL-2, we next examined the effect of Daclizumab on their IL-2-induced tyrosine phosphorylation.
Goebel et al. (2000)IL-2In immunoblots of IL-2R beta- and gamma-chain-immunoprecipitates the tyrosine phosphorylation of both chains by IL-2, but not by IL-15, was attenuated in the presence of Daclizumab.
Ishii et al. (1987)IL-2In IL-2-dependent cells, IL-2 dose responses of pp67 phosphorylation and cell proliferation were exactly correlated.
Stancato and Petricoin (2001)IL2With this method, we identified known proteins phosphorylated in response to IL2 or IFNalpha using both silver staining and Western blotting for protein detection/identification.
Hamann and Anderer (1989)Interleukin 2-inducedmononuclear cellsInterleukin 2-induced protein phosphorylation/dephosphorylation in human non-adherent peripheral blood mononuclear cells: initial phosphorylation of two 75-kDa proteins.
Hamann and Anderer (1989)IL-2Moreover, PHA pretreatment of cells induced a drastic phosphorylation of a 48-kDa (pI 6.5) and a 46-kDa (pI 6.7) protein, which were gradually dephosphorylated after IL-2 addition.
Einspahr et al. (1992)IL-2Furthermore, in response to IL-2, p56lck itself undergoes complex alterations in serine and tyrosine phosphorylation.
Paliogianni et al. (1993)IL-2-dependentT cellInhibition of T cell proliferation by Dex was associated with decreased IL-2-dependent tyrosine phosphorylation of several intracellular proteins and decreased phosphorylation of the retinoblastoma gene product Rb, a protein essential for controlling the progression of cells through the cell cycle.
Sabe et al. (1984)IL-2The results indicate that the high and low affinity states of the IL-2 receptor are not solely determined by phosphorylation of the receptor.
Asao et al. (1992)IL-2IL-2-dependent in vivo and in vitro tyrosine phosphorylation of IL-2 receptor gamma chain.
Volkland et al. (2007)IL-2Although MT204 did not prevent IL-2 from binding to CD25, it potently antagonized downstream signaling events of IL-2 at sub-nanomolar concentrations, such as STAT3 tyrosine phosphorylation, expression of CD124, production of gamma-interferon and cell proliferation.
Evans et al. (1994)IL-2Analysis of signal transduction events shows that pervanadate induces the activity of the src family of tyrosine kinases lck and fyn and the tyrosine phosphorylation of a major IL-2 responsive protein of 97 kDa.
Evans et al. (1994)IL-2Pervanadate does not, however, induce the activity of tyrosine kinases associated with the IL-2 receptor or the phosphorylation of a major IL-2 responsive protein of 116 kDa (Jak-3).
Adunyah et al. (1997)IL-2We report that IL-15 induces tyr phosphorylation of the p75IL-2R beta and p64IL-2R gamma subunits and Shc.
Dorsch et al. (1994)IL-2However, a 55- and a 155-kDa protein were only phosphorylated after IL-2 stimulation.
Dorsch et al. (1994)IL-2The 55-kDa protein specifically phosphorylated by IL-2 could be identified as p52shc which has recently been shown to be critically involved in Ras activation.
Nishio et al. (1994)interleukin-2Intracellular signal transduction has been reported to be triggered by phosphorylation of interleukin-2 (IL-2) receptor by IL-2.
Nishio et al. (1994)IL-2In order to clarify the effect of tyrosine phosphorylation of IL-2 receptors on cell-mediated cytotoxicity by natural killer (NK) cells, we studied the effect of a tyrosine kinase inhibitor, genistein, on the lethal effects of NK-rich cells for K562 cells.
Fung et al. (2003)IL-2-triggeredMoreover, these inhibitors significantly reduce IL-2-triggered STAT3 serine phosphorylation without affecting STAT5 serine phosphorylation.
Ferris et al. (1989)IL2These results suggest that signal transduction pathways for IL2 and IL3 involve tyrosine phosphorylation of protein substrates common to both lymphoid and myeloid linages.
Dorsch et al. (1994)IL-2Taken together our results indicate that the IL-2- and IL-7-stimulated intracellular pathways are partially different and that Shc is a target of IL-2-, but not IL-7-, stimulated tyrosine phosphorylation.
Morelon et al. (1996)IL-2This mutation does not affect high-affinity IL-2 binding, but it partly decreases IL-2 endocytosis and prevents the downmodulation of the IL-2-receptor beta chain and the tyrosine phosphorylation of Jak 3 protein in response to IL-2.
Ogawa et al. (1987)interleukin 2T cellTyrosine phosphorylation of an interleukin 2 receptor-like protein in cells transformed by human T cell leukemia virus type I.
Lanier et al. (1988)IL-2T lymphocytesIn this study, we describe an activation antigen, Leu-23, that is rapidly induced and phosphorylated after IL-2 stimulation of NK cells and a subset of low buoyant density T lymphocytes.
Gallis et al. (1986)interleukin-2Phosphorylation of the human interleukin-2 receptor and a synthetic peptide identical to its C-terminal, cytoplasmic domain.
Gallis et al. (1986)IL-2The recombinant IL-2 receptor in these cells was rapidly phosphorylated in response to phorbol myristate acetate (PMA), but its phosphorylation could not be detected in the absence of PMA or upon addition of human IL-2.
Gallis et al. (1986)IL-2These data suggest that protein kinase C, in response to PMA, phosphorylates the C-terminal serine residue (serine 247) in the human IL-2 receptor.
Migone et al. (2001)IL-2Cytokines, such as interleukin-2 (IL-2), activate intracellular signaling pathways via rapid tyrosine phosphorylation of their receptors, resulting in the activation of many genes involved in cell growth and survival.
Saltzman et al. (1989)IL-2-dependentIL-2-dependent tyrosine phosphorylation was observed in both cell lines and the concentrations of IL-2 needed to stimulate this phosphorylation were similar to that required for binding to the p75 receptor.
Vitte-Mony et al. (1994)IL-2NK cellsWestern blot analyses, and immunoprecipitations of cell lysates from 32P-preloaded NK cells demonstrated that seven major proteins are tyrosine phosphorylated in response to IL-2.
Rocha-Zavaleta et al. (2004)IL-2Moreover, inhibition of IL-2Rbeta phosphorylation by blocking c-Kit tyrosine kinase activity abolishes both, IL-2 and SCF-mediated proliferation.
Michiel et al. (1991)IL-2Interleukin 2 (IL-2) has been shown to stimulate tyrosine phosphorylation of a number of proteins requiring only the p75 beta chain of the IL-2 receptor.
Benedict et al. (1987)IL 2The 78 kd molecule is phosphorylated in vitro within the immune complex only in the presence of exogenously added IL 2, whereas the 57 kd molecule is phosphorylated equally in the presence or absence of IL 2.
Ferris et al. (1989)IL-2-stimulatedlarge granular lymphocytesAnti-Tac mAb could inhibit IL-2-stimulated tyrosine phosphorylation in activated T cells, which express both IL-2R subunits that together form the high affinity receptor complex, but not in large granular lymphocytes expressing only the IL-2R beta subunit.
Brockdorff et al. (2001)IL-2T cellsPreincubation of cells with the Src family kinase inhibitor, PP1, surprisingly increased the IL-2- and IL-15-induced tyrosine phosphorylation of Gab2, indicating that an Src family kinase member negatively regulates IL-2 receptor signaling in MF T cells.
Lansigan et al. (2010)IL2IL2RHistory of IL2, IL2R, and anti-Tac
Ross et al. (2010)IL-2-inducedAlthough the mechanisms governing IL-2-induced tyrosine phosphorylation and activation of JAK3/STAT5 have been extensively studied, the role of serine/threonine phosphorylation in controlling these effectors remains to be elucidated.
Ross et al. (2010)IL-2-inducedPP2AMoreover, inhibition of PP2A, but not PP1, diminished IL-2-induced tyrosine phosphorylation of IL-2Rbeta, JAK3, and STAT5, and abolished STAT5 DNA binding activity.
Goebel et al. (2001)IL-2T lymphocytesRESULTS: In human T lymphocytes, 16H5 attenuated both the tyrosine phosphorylation of STAT5 by IL-2 and the IL-2-induced DNA-binding of this transcription factor.
Goebel et al. (2001)IL-2In contrast, 16H5 had no effect on the serine phosphorylation of Akt by IL-2 or on the IL-2-induced DNA-binding of myc.
Campbell et al. (1990)rhIL-2Also, rhIL-2 underwent slow autophosphorylation by an intramolecular mechanism using [gamma-32P]8N3ATP as well as nonphotoactive nuceotide.
Migone et al. (1998)IL-2Interleukin 2 (IL-2) rapidly induces tyrosine phosphorylation of intracellular substrates, including the IL-2 receptor beta chain (IL-2Rbeta), Janus kinase 1 (Jak1), Jak3, signal transducer/activator of transcription proteins, and Shc, but the mechanism underlying dephosphorylation of these proteins is not known.
Guyot et al. (1998)IL-2However, while neither IL-2 nor CD3 cross-linking alone induced P-CREB, a 4.5-fold increase in phosphorylation of CREB within 1 h of IL-2/CD3 co-stimulation was observed.
Kabelitz (2010)IL-2T cellsT cells (by N-BP or phosphoantigen plus IL-2) followed by the adoptive transfer of in vitro expanded ??
Forrest et al. (2005)IL-2Moreover, exogenous IL-2 induced characteristic tyrosine phosphorylation events detectable by immunoblotting in these cells.
Podtschaske et al. (2007)IL-2bandsIntegrated fluorescence intensity of both NFATc2 bands showed a stronger NFATc2 dephosphorylation in the IL-2 positive fraction (66.5%) than in the IL-2 negative fraction (37.6%).
Chen and Dong (1997)IL-2The addition of IL-2 induced apoptosis of PWM-pretreated U937 cells.
Castro et al. (1999)IL-2IL-4 suppressed activation of DNA binding and tyrosine phosphorylation of the transcription factor Stat5 by IL-2, and suppressed the expression of the IL-2-inducible genes CD25, CIS, the PGE2 receptor, and cytokine responsive (CR) genes CR1 and CR8.
Shackelford and Trowbridge (1986)interleukin-2Phosphorylation of the interleukin-2 receptor, class I HLA antigens, and T200 glycoprotein.
Shackelford and Trowbridge (1986)IL-2T lymphocytesIL-2 receptors on normal human T lymphocytes and the leukemic cell line, HUT102B2, are rapidly phosphorylated in vivo in response to the tumor-promoting phorbol ester, 12-O-tetradecanoylphorbol 13-acetate (TPA).
Shackelford and Trowbridge (1986)IL-2Tryptic peptide analysis showed that the in vitro and in vivo 32P-labeled IL-2 receptors were phosphorylated on the same sites.
Shackelford and Trowbridge (1986)IL-2These results also provide direct evidence that the in vivo phosphorylation of the IL-2 receptor stimulated by TPA is catalyzed by protein kinase C.
Holter et al. (1994)IL-2We found that tyrosine phosphorylation in response to IL-2 in HUT78 cells was strongly inhibited by a short preincubation with TGF-beta 1.
Ahuja et al. (1993)IL-2-mediatedThis inhibition was associated with the down-regulation of IL-2-mediated tyrosine phosphorylation of proteins of 120, 100, 85, 75, and 50 kDa.
Shackelford and Trowbridge (1986)IL-2From these studies, it was concluded that Ser-247 is the major site of phosphorylation in the IL-2 receptor and that Thr-250 is a minor site.
Truitt et al. (1994)IL-2Interleukin-2 (IL-2) signaling results in tyrosine phosphorylation of the 75-kDa IL-2 receptor (IL-2R) beta chain and the activation of phosphatidylinositol 3'-kinase (PI3-K).
Zu et al. (1990)IL 2Also, in other IL 2 independent cell lines, such as KUT-2 and HUT-102, constitutive phosphorylation of the 65-kDa protein occurred without IL 2-stimulation.
Kung et al. (1989)IL-2Phosphorylated IL-2 synthesized in E. coli was analyzed by SDS-PAGE, reverse phase HPLC, and tryptic peptide mapping.
Aluoch and Whalen (2006)IL-2These data suggest that IL-2 and/or IL-12-mediated recovery of NK cytotoxic function is not a result of prevention/reversal of TBT-induced phosphorylation of p38 and p44/42 MAPKs at the 24 or 48 h time points.
Fleury et al. (1995)IL-2Such systems could constitute a sustained release form of IL-2 for immunotherapy.
Mills et al. (1990)Interleukin 2Interleukin 2-induced tyrosine phosphorylation.
Asao et al. (1990)IL-2Interleukin 2 (IL-2)-induced tyrosine phosphorylation of IL-2 receptor p75.
Brunetti et al. (2002)IL-2Proliferation, glucocorticoid (GC) receptor binding, anti-CD3-induced tyrosine phosphorylation, IL-2 production, and expression of IL-2 receptor alpha, beta, and gamma chains were evaluated.
Rani et al. (1992)IL-2Immunotherapy in the form of IL-2 can be suggested in such patients.
Einspahr et al. (1992)Interleukin-2NK cellsInterleukin-2 signal transduction in human NK cells: multisite phosphorylation and activation of the tyrosine kinase p56lck.
Evans et al. (1992)IL-2We found that PHA did not stimulate RB phosphorylation within 10 h of treatment, but IL-2 could effectively stimulate RB phosphorylation within 2 h, and this approached a maximum within 8-10 h of IL-2 treatment.
Evans et al. (1992)IL-2-stimulatedFurther, by using actinomycin D to inhibit new gene transcription following IL-2 stimulation, we found that early-cell-cycle phosphorylation of RB required IL-2-stimulated gene transcription.
Flescher et al. (1994)IL-2We found that polyamine oxidase activity suppresses both anti-CD3-induced IL-2 production and protein tyrosine phosphorylation.
Henderson et al. (1983)IL 2The most abundant form of IL 2 had an apparent m.w. of 16,000 and an isoelectric point of 6.3.
Farrar and Ferris (1989)IL 2Previously, it was shown that IL 2 stimulates rapid serine phosphorylation of several membrane and cytysolic proteins.
Farrar and Ferris (1989)IL 2T lymphocytesHere, using anti-phosphotyrosine antibodies to purify phosphotyrosyl proteins and two-dimensional gel analysis, we show that IL 2 stimulates rapid tyrosine phosphorylation of a variety of cellular proteins, including pp180, pp92, and pp42 in activated human T lymphocytes.
Goebel et al. (2000)IL-2-inducedHowever, after IL-2 stimulation (200 U/ml, 20 min), immunoblots of cell lysates demonstrated attenuation of the IL-2-induced tyrosine phosphorylation of 65-75 kDa proteins by Daclizumab, but not by isotype controls.
Ishii et al. (1987)interleukin 2T-cellsCharacterization of interleukin 2-stimulated phosphorylation of 67 and 63 kDa proteins in human T-cells.
Ishii et al. (1987)interleukin 2T-cellWe have investigated rapid and marked phosphorylation of cellular proteins induced by interleukin 2 (IL-2) in both phytohaemagglutinin-stimulated normal peripheral blood leucocytes, and IL-2-dependent or -independent human T-cell lines bearing human T-cell leukaemia (lymphotropic) virus type I.
Ishii et al. (1987)IL-2IL-2 also stimulated phosphorylation of four other proteins, with an Mr of 63,000 and pI values 5.3-6.1 (pp63s).