Viewing negative mentions of binding of IL2: interleukin 2 (Homo sapiens)

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Haynes and Cohen (1993)IL-2IL-2Finally, despite the biological and physical similarities between Xenopus TCGF and mammalian IL-2, anti-human IL-2 monoclonal antibodies do not recognize Xenopus TCGF.
Bormann et al. (1989)interleukin 2glycophorinSynthetic glycophorin C and interleukin 2-receptor Tac antigen transmembrane peptides, although similar in amino acid composition, did not interact with glycophorin and did not inhibit the binding of the synthetic glycophorin A transmembrane peptide to native glycophorin.
Harel-Bellan et al. (1989)IL-2T cellsFluoresceinated IL-2 did not bind to normal resting T cells, whereas a highly significant binding was observed on PHA-activated human T cells.
Smith et al. (1989)IL-2The inhibitor does not appear to bind to IL-2 or the IL-2 receptor.
Najjam et al. (1998)IL-2Human IL-2 analogs with single amino acid substitutions at positions Lys43, Thr51, and Gln126 analogs no longer bind to heparin.
Kamio et al. (1992)IL-2No IL-2 binding was detectable in the presence of anti-Tac antibody in these cells.
Ogura et al. (1988)IL-2The ternary complex with human IL-2 was formed on EL/Tac 3 cells expressing human L and murine H chains, although human IL-2 was unable to bind to the parental EL-4 cell, which does not express human L chain.
Emerson et al. (2003)IL-2NMR characterization of interleukin-2 in complexes with the IL-2Ralpha receptor component, and with low molecular weight compounds that inhibit the IL-2/IL-Ralpha interaction.
Alileche (2003)IL-2immune cellsWe have also to understand that IL-2 can interact directly with cancer cells and also with stromal cells (endothelial and fibroblastic cells), the outcome of IL-2 immunotherapy is not restricted to the interactions with immune cells.
Ide et al. (1987)IL-2All the four MAbs were specific to human IL-2: they did not cross-react with mouse or rat IL-2.
Mingari et al. (1985)IL2IL2B cellMoreover, the effect of such spleen supernatant on B cell proliferation correlated with the IL2 activity since its B cell growth factor activity (BCGF) was not greater than that of purified IL2 and no residual BCGF activity could be detected after absorption of all IL2 activity by the IL2-dependent cytotoxic T lymphocyte line cells.
Kramer and Koszinowski (1982)IL 2T cellsFurthermore, SF inhibited the release of IL 2 from producer T cells but had no detectable effect on the interaction of IL 2 with receptive T cells.
Robb et al. (1981)TCGFTCGFT cellIn contrast, a number of TCGF-independent cell lines of T cell, B cell, or myeloid origin did not bind detectable quantities of radiolabeled TCGF.
Noma et al. (1990)IL2IL2lymphocytesNormal lymphocytes stimulated with OVA expressed Tac antigen (low affinity IL2 receptor) but, in contrast to those from the allergic patients, did not absorb nor respond to IL2.
Aszalos et al. (1987)IL-2IL-2The cell population that did not effectively bind dans-CsA lacked IL-2 receptors and did not respond to CsA or IL-2 with immediate membrane potential changes.
Noma et al. (1990)IL2Tac antigenlymphocytesNormal lymphocytes stimulated with OVA expressed Tac antigen (low affinity IL2 receptor) but, in contrast to those from the allergic patients, did not absorb nor respond to IL2.
Saito et al. (1991)IL-2lymphocyteAlthough the H (beta) chain expressed on lymphocytes can bind IL-2 with a Kd value of 1 nM (intermediate affinity), transfected fibroblasts expressing the H chain cannot bind IL-2, suggesting the involvement of other lymphocyte-specific factors for the function of the H chain.
Saito et al. (1991)IL-2lymphocytesWe found that lysates of transfected Cos7 cells expressing H chains can bind IL-2 when mixed with lysates from lymphocytes that cannot bind IL-2.
Aszalos et al. (1987)IL-2CsAThe cell population that did not effectively bind dans-CsA lacked IL-2 receptors and did not respond to CsA or IL-2 with immediate membrane potential changes.
Lee et al. (1990)IL-2IL-4-mediated inhibition of IL-2-induced IL-2R expression does not seem to be due to the direct interaction between IL-4 and cell surface receptors, inasmuch as preincubation of cells with IL-4 for 60 min at 37 degrees C did not alter the binding of 125I-IL-2 to cells previously cultured for 3 days with SAC plus IL-2.
Croze et al. (1988)IL-2IL-2IL-2 and hGH elicit their growth stimulation through different receptors since IL-2 does not compete with 125I-hGH for binding to Nb2 cells and Met14hGH, an antagonist of hGH, inhibits the hGH-stimulated growth of Nb2 cells but not that caused by IL-2.
Grimm et al. (1983)IL-2anti-Tac antibodyThe LAK activation factor was directly and consistently associated with IL-2 activity using classic protein purification techniques, adsorption to IL-2-dependent cell lines, and inhibition with anti-Tac antibody.
Smith et al. (2007)IL-2T cellFor CD4+ T cell concentrations, statistical analysis showed no interaction between IL-2 and vaccine, but there was a main effect of IL-2 (p < 0.0001) and no effect of vaccine.
Emerson et al. (2003)IL-2IL-RalphaNMR characterization of interleukin-2 in complexes with the IL-2Ralpha receptor component, and with low molecular weight compounds that inhibit the IL-2/IL-Ralpha interaction.
Choi and Yoo (2002)rhIL-2rpIL-2However, anti-rpIL-2 antibody did not recognize rhIL-2, and anti-rhIL-2 antibody also did not react with rpIL-2 in the same assay.
Najjam et al. (1998)IL-2In addition soluble IL-2 receptor alpha and beta polypeptides do not compete with heparin for the binding of IL-2.
Leonard et al. (1984)interleukin-2We have isolated an additional cDNA that may correspond to an alternatively spliced mRNA that lacks a 216 base segment and appears to encode an altered membrane protein which cannot bind interleukin-2.
Matsuzaki et al. (1989)IL-2T cellAbsorption experiments with the factor and Con A-induced T cell blasts as well as [125I]IL-2 binding experiments with T cell blasts revealed that the factor acted on the physiological events occurring after IL-2-mediated stimulation of IL-2 receptor complexes, demonstrating no interaction of the factor with either IL-2 molecules or IL-2 receptor complexes.
Kikuchi et al. (2002)IL-2 geneFurthermore, no association was observed between IL-2 gene polymorphisms and clinical characteristics, such as clinical course and age at disease onset.
Hellstrand et al. (2000)IL-2NK cellHistamine inhibits ROS formation in MO via H2-receptors; thereby, histamine protects NK cells from MO-mediated inhibition and synergizes with IL-2 and IFN-alpha to induce killing of NK cell-sensitive human tumor cells in vitro.
Wang and Norman (1992)IL-2plasmaThere was no association between IL-1, IL-2 and steroid levels, regardless of whether they were compared in follicular fluid or plasma.
Tanaka et al. (1991)IL2TILFreshly isolated TIL cultured with autologous tumor cells for 48 h without IL2 were small, round and showed neither binding to nor killing of tumor cells.
Fenton et al. (1996)IL-2A third patient who was DTH(+) after vaccination with no IL-2 had a dramatic PR after receiving IL-2 subcutaneously in a subsequent protocol.
Itoh et al. (1994)IL-10IL-2bloodHighly purified peripheral blood B cells from normal human individuals were cultured with Staphylococcus aureus Cowan I (SA) in the presence or absence of IL-10 and IL-2.
Clark et al. (2000)IL-2Intratumoral granzyme A activity was significantly increased in tumors injected with IL-2 plasmid:DMRIE/DOPE complexes, but not by an irrelevant plasmid DNA:DMRIE/DOPE control.
Allegretta et al. (1986)lymphokineinterleukin-2These results suggest that the anti-interleukin-2 serum antibodies generated in the course of treatment do not react with the nonrecombinant lymphokine but recognize epitopes peculiar to recombinant forms which are not dependent on the amino acid substitution at position 125.
Chakraborty and Mandal (1993)interleukin-2T-helper cellsThese results establish a cross species inhibitory effect of human AFP and it may be stated that this effect is directly targeted on T-helper cells and has no interaction with interleukin-2 (IL-2).
Sperandio et al. (2008)IL-2In this macromolecular complex, the presence of an equilibrium between several conformations has been observed and small molecules have been determined that prevent the interaction between IL-2 and its receptor IL-2R?
Robb et al. (1981)TCGFT cellIn contrast, a number of TCGF-independent cell lines of T cell, B cell, or myeloid origin did not bind detectable quantities of radiolabeled TCGF.
De Groot et al. (1990)IL-2High-density fractions contain cells with a "resting" phenotype which after in vitro activation proliferate with IL-4 but not with IL-2.
Aszalos et al. (1987)IL-2The cell population that did not effectively bind dans-CsA lacked IL-2 receptors and did not respond to CsA or IL-2 with immediate membrane potential changes.
Kaufmann et al. (1987)IL 2PBLIn contrast to normal PBL that generated LAK effectors when their proliferation was inhibited, the irradiated, nonproliferating T-ALL leukemic cells did not respond to IL 2.
Karmann et al. (1996)IL-2However, increased IL-2 secretion in the presence of EC can not fully account for endothelial-induced CD40 ligand up-regulation as (1) the effect of exogenous IL-2 is greater at 24 h than at 6 h, whereas the opposite is true for EC; (2) the effect of saturating levels of IL-2 is considerably smaller than that of EC; and (3) blocking of IL-2 receptors does not fully inhibit endothelial effects on CD40 ligand expression.
Rice et al. (1992)IL-2lymphocyteThe addition of exogenous IL-2 did not counteract lymphocyte suppression.
Heaton and Grimm (1995)IL-2We assessed the roles of the various receptor complexes in these responses to IL-2 using antibodies that block the interaction of IL-2 with the intermediate-affinity IL-2R (humanized Mik beta 1) and high- and low-affinity interactions (HTac).
Okadome et al. (1991)IL-2HeLaA significant antiproliferative activity of the lymph node cells (LNCs) against a cervical cancer cell line, HeLa cells, was demonstrated by stimulation with either phytohemagglutinin (PHA) or concanavalin A (Con A), but not with interleukin-2 (IL-2).
Saito et al. (1991)IL-2anti-H chain antibodyChemical cross-linking of 125I-IL-2-bound lysate mixture and subsequent immunoprecipitation with a noncompetitive anti-H chain antibody gave rise to two 125I-IL-2-bound proteins, a 56-kDa protein (p56) and the H chain, although neither the H chain nor p56 alone is able to bind IL-2.
Chouaib et al. (1985)IL 2T lymphocytesIt was demonstrated that isoproterenol, as well as dBcAMP, inhibited transferrin receptor expression on PHA-activated T lymphocytes to the same extent as PGE2, and exogenous IL 2 could not counteract the down-regulation of the receptor expression.
Boyd et al. (1985)IL 2B cellsMoreover, activated B cells could be induced to proliferate by IL 2, but not to secrete Ig.
Piscitelli et al. (1996)Interleukin-2Interleukin-2 and alpha interferon do not alter the pharmacokinetics of zidovudine and didanosine, respectively.
Jacques et al. (1987)IL-2Whereas 2.5% of cell-surface IL-2R expressed 2 days after antigenic stimulation of 2B11 cells were of high affinity for IL-2 (KD = 25 pM), no (less than 0.07%) high affinity binding sites could be detected on the purified soluble IL-2R.
Kuida et al. (1992)IL-2RIL-2lymphoblastsIn the present study, we observed that human IL-2R beta expressed in a mouse myeloma X63-Ag8.653 (X63) by cDNA transfection did not bind IL-2, while the same IL-2R beta expressed in an IL-6-dependent mouse B cell hybridoma F12-28, which was obtained by cell fusion between X63 and lipopolysaccharide (LPS)-induced lymphoblasts, bound IL-2 with the intermediate affinity.
Deane et al. (2000)IL-2GIF did not bind human GM-CSF or IL-2 in spite of the fact that orf virus is a human pathogen.
Recchia et al. (2002)IL-2Interleukin-2 (IL-2) which has no cross-resistance with chemotherapy, has given responses in tumors resistant to chemotherapy, and shown to be more effective when tumor burden is low. 13-cis retinoic acid (RA) has immunomodulatory properties, potentially synergistic with IL-2.
Collins et al. (1988)interleukin 2Substitution of Asp20 or deletion of Phe124 resulted in inactive analog proteins that were unable to interact with the high-affinity p55/p70 complex or the intermediate-affinity p70 subunit of the interleukin 2 receptor.
Zorn et al. (1994)IL-2These last findings indicate that there is at least no functional interaction between sIL-2R and free IL-2, whereas an interaction of sIL-2R with the membrane-bound receptor for IL-2 seems possible.
Ampel et al. (2002)IL-2MFI CD69Among 30 subjects for whom data were available, there was a highly significant association between the MFI CD69 above control and the supernatant concentrations of gamma interferon, interleukin-2 (IL-2), and tumor necrosis factor alpha (for all, P < 0.001), but not for IL-4, IL-5, or IL-10.
Rickert et al. (2004)IL-2We find an entropically favorable high affinity interaction between IL-2 and its alpha receptor, a moderately entropically favorable low affinity interaction between IL-2 and its beta receptor, and no interaction between IL-2 and the shared receptor, gamma(c).
Nicolas et al. (1987)IL-2ETAFthymocyteThis factor, which is distinct from prostaglandins, epidermal cell-derived thymocyte activating factor (ETAF), and the well-known thymic hormones (thymulin, thymopoietin, and thymosin alpha 1) did not exhibit any interleukin (IL)-1, IL-2, or IL-3 activity.
Nicolas et al. (1987)IL-2thymulinthymocyteThis factor, which is distinct from prostaglandins, epidermal cell-derived thymocyte activating factor (ETAF), and the well-known thymic hormones (thymulin, thymopoietin, and thymosin alpha 1) did not exhibit any interleukin (IL)-1, IL-2, or IL-3 activity.
Recchia et al. (2001)IL-2IL-2 has no cross-resistance with chemotherapy and improves cancer-related lymphocytopenia, a factor that determines poor prognosis, whereas RA has immunomodulatory properties, potentially synergistic with IL-2.
Abolhassani and Chiao (1991)IL-2In ELISA assay antibodies against IL-1, IL-2, IL-3 and IL-6 did not recognize the LGEF preparation.
Mingari et al. (1985)BCGFIL2B cellMoreover, the effect of such spleen supernatant on B cell proliferation correlated with the IL2 activity since its B cell growth factor activity (BCGF) was not greater than that of purified IL2 and no residual BCGF activity could be detected after absorption of all IL2 activity by the IL2-dependent cytotoxic T lymphocyte line cells.
Rajasekaran et al. (2010)IL-2effector T cellsCD28Lo effector T cells did not generate any IL-2 with anti-CD3 mAb activation (Figure 6F).
Deane et al. (2000)GIFIL-2GIF did not bind human GM-CSF or IL-2 in spite of the fact that orf virus is a human pathogen.
Marrack et al. (1983)interleukin 2It was found that subclones of the hybridoma that no longer secreted interleukin 2 in response to Ag/H-2, even though they continued to secrete interleukin 2 in response to concanavalin A, also no longer bound specifically to Ag-pulsed monolayers of the appropriate H-2 type.
Eicher et al. (2002)IL-2-inducibleSingly or co-transfected IL-2Rbeta and gamma(c) chimeras showed no spontaneous or IL-2-inducible oligomerization.
Shao et al. (2000)IL-2T cellThe enhancing activity does not correspond to IL-2, IL-15, nor to several other cytokines implicated in T cell proliferation/activation.
Granelli-Piperno and Nolan (1991)IL-2T cellsAll factors are induced in the nuclei of T cells upon activation with mitogens but not with exogenous IL-2 growth factor.
Chu and Sharom (1993)IL-2The glycolipids also prevented chemical cross-linking of [125I]IL-2 to the p55/p75 complex, as well as to both IL-2R alpha (p55) and IL-2R beta (p75) independently.
Tanaka et al. (1987)IL2mononuclear cellsThe in vitro production of human interleukin 1 alpha (hIL 1 alpha) and interleukin 1 beta (hIL 1 beta) by peripheral blood mononuclear cells was examined by sensitive sandwich enzyme immunoassays which could discriminate hIL 1 alpha and hIL 1 beta without cross-reaction with human IL2.
Takeshita et al. (1992)IL-2HeLaIn addition, neither p64 co-precipitation nor IL-2 binding was detected in HeLa and COS7 cells transfected with IL-2R beta, and no p64 precipitation was seen even in those transfectants with both IL-2R alpha and beta cDNAs, which bind to IL-2 with high affinity but are not able to transduce intracellular signals.
Neeper et al. (1987)IL 2Tac protein missing amino acids 102 to 173 (exon 4) is unable to bind IL 2: detection of spliced protein after L cell transfection.
Hellstrand et al. (1994)IL-2LungLung melanoma did not respond to histamine/IL-2/IFN alpha.
Kuida et al. (1992)IL-2fibroblastsAlthough IL-2 receptor beta chain (IL-2R beta) expressed in various lymphoid cell lines binds IL-2 with an intermediate affinity, IL-2R beta expressed in fibroblasts is unable to bind IL-2, suggesting that IL-2R beta is on its own not sufficient for generating the intermediate-affinity receptor and that lymphoid-specific regulatory control may be operated to allow IL-2R beta to bind IL-2.
Kuida et al. (1992)IL-2lymphoblastsInterestingly, when the human IL-2R beta cDNA-transfected X63 clone, which by itself manifests no IL-2 binding, was fused with LPS-induced lymphoblasts, the resultant hybridomas manifested intermediate-affinity IL-2 binding.
Rickert et al. (2004)IL-2gamma(c)We find an entropically favorable high affinity interaction between IL-2 and its alpha receptor, a moderately entropically favorable low affinity interaction between IL-2 and its beta receptor, and no interaction between IL-2 and the shared receptor, gamma(c).
Reed et al. (1985)IL 2T cellsWGA immobilized on support beads bound detergent-solubilized IL 2 receptors from PHA-activated T cells, but did not bind human IL 2.
Kuida et al. (1992)IL-2RIL-2fibroblastsAlthough IL-2 receptor beta chain (IL-2R beta) expressed in various lymphoid cell lines binds IL-2 with an intermediate affinity, IL-2R beta expressed in fibroblasts is unable to bind IL-2, suggesting that IL-2R beta is on its own not sufficient for generating the intermediate-affinity receptor and that lymphoid-specific regulatory control may be operated to allow IL-2R beta to bind IL-2.
Kuida et al. (1992)IL-2RIL-2lymphoblastsInterestingly, when the human IL-2R beta cDNA-transfected X63 clone, which by itself manifests no IL-2 binding, was fused with LPS-induced lymphoblasts, the resultant hybridomas manifested intermediate-affinity IL-2 binding.
Chen and Rothenberg (1994)IL-2This result, and similar results in cyclosporin A-treated cells, imply that individual IL-2 transcription factors cannot stably bind their target sequences in vivo without coengagement of all other distinct factors at neighboring sites.
Minamoto et al. (1990)IL-2RIL-2L929However, neither IL-2 internalization nor signal transduction via the high affinity IL-2R complex were observed in the L929 transformants.
Lonnemann et al. (1987)IL-2IL-1This material was neutralized by anti-human-IL-1 which does not recognize IL-2.
de Jong et al. (1996)IL-2Proliferation of anti-CD3 (OKT3)-stimulated human PBL was compared for responsiveness to IL-2, IL-15, and F42K, and IL-2 mutant that does not bind the IL-2R alpha chain.