Viewing negative mentions of positive regulation of IL2: interleukin 2 (Homo sapiens)

Full-text article links are indicated by after the article reference.

Document Target Regulator Anatomy Sentence
Welte et al. (1984)IL-2IL-2T cellOKT3 monoclonal antibody at low, nonmitogenic concentrations (25 pg/ml) or IL-2 alone at optimal concentrations (20 U/ml) did not induce IL-2 receptor expression, as measured by Tac antibody or by T cell proliferation.
Wacholtz et al. (1991)IL2lectinT cellsOther cyclic adenosine monophosphate (cAMP) elevating agents (forskolin, isoproterenol, and the cAMP analogue, dibutyryl cAMP) also inhibited lectin-stimulated IL2 production by T cells, but could not inhibit IL2 production by Jurkat cells.
Thoman et al. (1984)IL 2Con AlymphocyteIn addition, synthesis of IL 2 in mixed lymphocyte cultures is blocked by C3d-K, but not IL 2 synthesis induced by Con A.
Holán et al. (1991)IL-2IFN-alphaIt was found that IFN-alpha alone did not stimulate IL-2 production, but increased it in the activated cells.
Aihara et al. (1999)IL-2Candida antigenCandida antigen induced IL-2 secretion in the normal and the non-asthmatic subjects, but not in the asthmatics.
Tomita et al. (1988)IL-2PWMHSFWe demonstrated that HSF inhibited PWM-induced IL-2 production by CD4+ cells but not by CD8+ cells and its suppressive activity on Ig production was totally abrogated by preabsorption with CD4+ cells, but not by CD8+ cells.
Smyth et al. (1990)IL-2IL-6T cellsThe costimulatory effect of IL-6 did not appear to involve the IL-2/IL-2R pathway in as much as IL-6 did not induce IL-2 production or detectably increase IL-2R surface expression in T cells.
Dao et al. (1994)IL-2IFN-alphaCTLL-2IFN-alpha alone had no effect on either the IL-2 production or apoptosis of MOLT-16 cells, but significantly increased both the IL-2 production and apoptosis in the MOLT-16 cells after phytohemagglutinin (PHA) stimulation as determined by CTLL-2 assay and by flow cytometric analysis using propidium iodide (PI) staining.
Clevers et al. (1985)IL-2It is concluded that A23187 induces the expression of IL-2 receptors without concurrent stimulation of IL-2 production, thus allowing only low levels of proliferation.
Medveczky et al. (1993)interleukin-2lymphocytesWe show in this report that infection of human peripheral white blood cells with a group C strain 484-77 results in selective expansion of CD8 lymphocytes with strong cytotoxic activity and these cells do not require interleukin-2 (IL-2) for growth.
Lindqvist et al. (1987)IL-2PBLThese results indicate that a cell-to-cell adhesion step is necessary for the production of IL-2, but not for the expression of its receptor on both PHA- and P(Bu)2-treated human PBL.
Tsang et al. (1986)IL-2T cellHowever, no augmentation of IL-2 production was observed when cultures of HUT-78 cells, a human leukemic T cell line, were treated with ISO.
Kubbies et al. (1990)IL2lymphocytesUsing a novel flow cytometric assay (BrdU/Hoechst flow cytometry) for the visualization and quantitation of cell activation and cell progression through multiple cell cycles we show that (1) resting mononuclear cells from human venous blood respond to polyclonal activation in a highly asynchronous fashion, as some of these cells enter their first cell cycle as early as 30 and as late as 80 hrs after exposure to the mitogen; (2) reducing agents improve the mitogenic response of polyclonally activated human lymphocytes by increasing the recruitment of non-cycling G0/G1 cells; (3) of 8 reducing agents tested, alpha-thioglycerol proved most effective with respect to enhancing recruitment into the cell cycle; (4) the effective agents needs to be present permanently for maximum response; (5) the growth-promoting effect of alpha-thioglycerol is mediated, but not solely caused by increased IL2 production.
Roffman et al. (1984)interleukin 2lymphocytesChemical reduction of oxidized human lymphocytes inhibits interleukin 2 production but not induction of interleukin 2 responsiveness.
Ythier et al. (1985)IL2In fact, there was no measurable interleukin 2 (IL2) secretion and no significant increase in IL2 susceptibility following incubation with either PHA or anti-T11(2) plus anti-T11(3).
Wiranowska-Stewart and Hadden (1986)IL-2Alone they were unable to consistently induce IL-2 activity.
Paetkau et al. (1980)IL2leukocyteUV-irradiated stimulators are also unable to induce the normal production of IL2 which is observed in a mixed leukocyte culture.
Holán et al. (1994)IL-2MOLT-13However, DC itself did not stimulate IL-2 production in PBL or in MOLT-13 cells.
Holán et al. (1994)IL-2T cellIn two additional T cell lines tested, MOLT-16 and H-9, DC did not enhance mitogen-stimulated IL-2 production, but suppressed it when applied at 1 mA for more than 10 min.
Rossi et al. (1988)IL2The irradiation or the addition of indomethacin did not enhance the IL2 production in PB from B-CLL patients.
Donnelly et al. (1986)IL-2PBMCExposure of PBMC to NPT-15392 for longer periods (i.e. 24 h) did not result in higher levels of IL-2 production.
Venkataraman (1992)IL-2MNCsSimilar to freezing, L-leucine methyl ester (Leu-OMe) treatment (to eliminate IL-1 and prostaglandin E-2 (PGE-2)-secreting cells) also increased the IL-2-producing activities of fresh cells, but freezing no longer enhanced the production of IL-2 by Leu-OMe-treated cells, suggesting that (1) both the freezing process and Leu-OMe treatment have similar effects on IL-2 production, (2) the increased IL-2 secretion by frozen MNCs is independent of IL-1, and (3) inactivation of PGE-2-secreting cells during the freezing procedure is responsible for increased IL-2 secretion.
Philip et al. (1996)IL-2Transfections with the non-AAV plasmid containing the identical expression cassette as the AAV plasmid induced IL-2 expression in the tumor cell line but failed to produce IL-2 in primary tumor cells.
Silva et al. (1981)IL-2The release of IL-2 by TG cells did not require DNA synthesis or functional Rc gamma receptors, since positively selected TH cells produced IL-2, even though they lacked lymphoproliferative responses to PHA and antibody-dependent cell-mediated cytotoxicity.
Bensussan et al. (1984)IL-2T-cellWe find that Te-Ti triggering of suppressor clones (T8AC6, T8AC7 or T8RW) does not result in IL-2 production or T-cell proliferation and in contrast to inducer clones, also leads to a transient IL-2 unresponsive state.
Venuta et al. (1983)IL-2DaudiSince normal T cells do not require Daudi to produce IL-2 and since Daudi cells do not produce IL-2 under any conditions, we conclude that the cell responsible for IL-2 production in acute lymphatic leukemia is a leukemic T cell with an altered mechanism of IL-2 production at the level of the Pan T2 binding site.
Teodorczyk-Injeyan et al. (1988)IL2Bypassing the requirements for mitogen-induced increases in [Ca++]i, using the cation ionophore A23187, or activating PK-C with the phorbol ester 12-o-tetradecanoyl-phorbol-13-acetate (TPA), failed to significantly restore SPA-induced IL2 production in cell cultures from burned patients.
Wu and Yang (1991)IL-2PBMCLithium markedly enhanced IL-2 production by MLA144 and PBMC, but not by Jurkat.
Adams et al. (1994)IL-2umbilical veinUsing conventional mixed cell cultures and limiting dilution analyses, we demonstrate that "resting" allogeneic human umbilical vein endothelial cells (HUVEC) stimulate IL-2 production, but not proliferation of purified CD3+ PBMC.
Kasahara et al. (1989)IL2The kinetics of IL2 and IFN-gamma production were essentially similar for both lymphokines except that there was a faster accumulation of IFN-gamma mRNA than IL2 mRNA.
Hirano et al. (1984)IL 2lymphocytesTPA alone did not induce the production of IL 2 in human tonsillar lymphocytes but enhanced the PHA-induced IL 2 production by seven-fold.
Larsen (1990)IL-2T cellsSequential stimulation with PDBu and ionomycin failed to induce IL-2 production, IL-2R expression, and consequently proliferation of the T cells, indicating that T-cell activation requires simultaneous activation of protein kinase C (PKC) and elevation of cytosolic calcium.
Altomare et al. (1997)IL-2There was a slight but not significant increase in IL-2 concentrations after major operations.
Morioka et al. (1993)IL-2PBLF4 enhanced IL-2-induced proliferation and IL-2 receptor (Tac) expression of PBL without significant increase of IL-2 production.
Yamamoto et al. (1986)IL2 mRNAlymphocytesAddition of alpha-methylornithine and methylglyoxal bis (guanyl hydrazone), which inhibit polyamine synthesis, did not affect the induction of IL2 mRNA in the lymphocytes stimulated with PHA and TPA, indicating that polyamine synthesis is not necessary for IL2 mRNA induction.
Kwong and Teh (1987)IL-2spleenASHF did not increase the amount of IL-2 produced by irradiated B6 spleen cells but increased the amount of IL-2 produced in CBA anti-B6 cultures by 3.8-fold.
Kwong and Teh (1987)IL-2Clone 9Clone 9 cells or ASHF did not increase IL-2 production in D2-stimulated cultures.
Kees et al. (1994)IL-2Significantly, a signal provided by a low concentration of PMA (not leading to IL-2 induction by itself in the presence of calcium ionophore) was necessary.
Meuer and Meyer zum Büschenfelde (1986)IL 2T lymphocytesIn contrast, presensitized T lymphocytes do not require IL 1 for IL 2 production.
Viora and Camponeschi (1995)IL-2 genePBMCIL-2 gene expression in the drug-treated PBMC did not increase.
Matsuzaki et al. (1986)IL-2T-cellThis process was not a consequence of nonspecific IL-2 gene activation, which occurs in cells persistently infected with reovirus, because reovirus infection did not activate IL-2 secretion in T-cell hybridomas with other antigenic specificities.
Iho et al. (1985)IL 2monocytesFurthermore, 1,25(OH)2D3 suppressed interleukin 1 (IL 1) production of monocytes (Mo), and the agent-treated Mo were unable to promote IL 2 production of non-adherent cells (NAC).
Welte et al. (1987)IL-2T cellsThe IL-2 production could be enhanced by coculture with host PBMC frozen before transplant but not by stimulation with mitogenic amounts of OKT3 antibody, thus suggesting an in vivo activation of donor T cells or their precursors by host tissue.
Welte et al. (1987)IL-2In patients whose cells had ceased to produce IL-2 spontaneously or never produced this cytokine, neither coculture with host cells nor stimulation with OKT3 antibody thereafter induced IL-2 release through the first year posttransplant.
Song et al. (1993)IL-2PMA alone, which induced high levels of tyrosine phosphorylation of the ERK protein, neither induced any detectable IL-2 nor increased the level of tyrosine phosphorylation of the 100,000 MW protein.
Hara and Fu (1985)IL-2They failed to induce either interleukin 2 (IL-2) receptor expression or IL-2 secretion.
Hara and Fu (1985)IL-2T cellsTPA induced a low level of IL-2 receptor expression in monocyte-depleted T cells, without inducing IL-2 secretion.
Langkamp-Henken et al. (2000)interleukin 2lymphocyteCONCLUSIONS: Pharmacologic doses of arginine were well tolerated but did not enhance lymphocyte proliferation or interleukin 2 production in nursing home residents with pressure ulcers.
Abramson et al. (1983)IL2T cellsIn addition, the data suggest that Ia+ sonicates alone may suffice for induction of IL2 responsiveness, but not for endogenous IL2 production and subsequent proliferation by primed T cells.
Yu et al. (1998)IL-2Although PHA stimulation increased IL-2 release, the expression of IL-2R alpha and beta chains and the release of sIL-2R were not proportionately increased in BD.
Yeh et al. (1984)TCGFB lymphoblastoid cells or 12-O-tetradecanoylphorbol 13-acetate alone did not induce TCGF receptors, but they did exert a very marked synergistic effect with Concanavalin A.
Nihlmark and Lindqvist (1989)IL-2These results indicate that intact microtubules are necessary for PHA/OKT3-induced proliferation and IL-2 production, but not for IL-2-induced proliferation.
Bregani et al. (1995)IL-2plateletPlatelet number increased during IL-2 plus melatonin, even though not significantly; on the contrary, platelet number decreased during IL-2 alone.
Holán and Minowada (1993)IL-2T cellThe production of IL-2 in T cell line H9 was not enhanced by any of the cytokines used.
Zatz et al. (1984)T-cell growth factorT-cellIncubation of lymphocytes with thymosin alone failed to induce any production of T-cell growth factor.
Blank et al. (1987)IL-2lymphocyteTrophoblast was demonstrated to be unable to cause the production of interleukin-2 (IL-2) by allogeneic splenocytes in vitro in two ways: 1) The addition of lymphocyte-trophoblast culture-supernatant (LTC-SN) did not stimulate the proliferation of IL-2 dependent cytotoxic T lymphocyte (CTL-L cells; 2) When responder cells were cultured with heat-treated splenocytes (usually no CTL generation) an increase of CTL formation could be seen in the presence of mixed lymphocyte culture-supernatant (MLC-SN) but not of SN from the cultures in which trophoblast cells served as stimulators.
Keicho et al. (1993)IL-2lymphocytesEM had a suppressive effect on the proliferative response of human lymphocytes stimulated with mitogens and antigens, while EM had no effect on concanavalin A (Con A)-induced interleukin-2 (IL-2) production or IL-2R alpha (CD25) expression.
Kramer et al. (1986)interleukin 2EL4TCDD did not inhibit EL4 cell growth or affect phorbol ester induced growth inhibition, and failed to stimulate production of interleukin 2.
Umetsu et al. (1988)interleukin 2B cellsYet in contrast to allogeneic monocytes, allogeneic B cells failed to induce a rise in the intracellular calcium ion concentration and failed to cause interleukin 2 (IL2) receptor expression in Clone A1.
Darko et al. (1988)interleukin-2natural killer cellsPercent of total lymphocytes labeled as all T lymphocytes, all B lymphocytes, and natural killer cells did not differ in the two groups, nor did mitogen-induced interleukin-2 production.
Lei et al. (1994)IL-2plasmaThe lymph-to-plasma protein concentration ratio increased after IL-2 for thoracic duct but not for caudal mediastinal node lymph.
Santos et al. (1996)interleukin 2NK cellThe reason for this is unknown; however, it was not due to an increase in the percentage of NK cells, nor to an increase in interleukin 2 (IL-2) receptor expression, nor to IL-2 production. beta-carotene may be acting directly on one or more of the lytic stages of NK cell cytotoxicity, or on NK cell activity-enhancing cytokines other than IL-2, such as IL-12.
Mansfield et al. (1991)IL-2TILsThey also failed to induce IL-2 production in uncultured TILs, although anti-CD3 mAb, but not HC antibody, stimulated IL-2 production in peripheral blood mononuclear cells (PBMCs).
Baine et al. (1995)IL-2Conversely, calcium-independent anti-CD28 Ab and PMA-induced IL-2 production was resistant.
Yukioka et al. (1987)interleukin-2Polyamine biosynthesis is necessary for interleukin-2-dependent proliferation but not for interleukin-2 production or high-affinity interleukin-2 receptor expression.
Yukioka et al. (1987)IL-2These results indicate that polyamine biosynthesis is necessary for IL-2-dependent proliferation, but not for IL-2 production or IL-2 receptor expression.
Heeg et al. (1988)interleukin-2helper T cellsHigh-dose irradiated splenic stimulator cells show no endogenous interleukin-2 production but stimulate clonally developing helper T cells to produce interleukin-2.
Combe et al. (1987)IL-2bloodIndomethacin, phorbol myristate acetate and irradiation of suppressor cells increased IL-2 values in rheumatoid SF and peripheral blood but did not restore normal IL-2 production.
Kang et al. (1987)IL-2BGEF alone did not induce the production of IL-2.
Manié et al. (1993)IL-2Interestingly, the co-culture stimulated by (Fab')2 fragments of CD3 mAb displayed an enhanced IL-1 beta production without any increase of IL-2 secretion.
Wells et al. (1994)IL-2No significant increase in IL-6, IL-2, or hyaluronic acid was found in the sera.
Donati et al. (1992)IL-2It was observed that a single session of in vitro dialysis did not induce production of interleukin-2 (IL-2) and did not alter the expression of IL-2 receptor (IL-2R) on the cytoplasmic membrane and the secretion of soluble IL-2R, whereas it induced transcription of mRNA for IL-2R.
Murakawa and Sakane (1988)IL-2PMA alone did not stimulate IL-2 production but, in concert with PHA, induced IL-2 synthesis.
Lombardi et al. (1994)IL-2T cellDespite their high levels of B7 expression, the T cell clones, were unable to induce proliferation or IL-2 secretion by DR-restricted, antigen-specific T cells.
Shirakawa et al. (1989)IL2IL2RThe expression of IL2R on ATL cells was also enhanced by IL1, but the production of IL2 was not induced.
Perrin et al. (1988)IL 2On the contrary, infection with a live rabies virus does not specifically induce the production of IL 2: splenocytes from ill mice previously infected with wild rabies virus cannot be specifically stimulated by rabies antigens, whereas they can be non-specifically stimulated by a mitogen (Concanavalin A (Con A].
Madden et al. (1999)IL-2bloodWhilst anti-CD3 stimulation did not induce TH1 cytokine production in blood T cells, it readily induced both IFN-gamma and IL-2 production in BAL T cells.
Roth and Dröge (1991)IL 2In contrast to the DNA synthesis activity in these cell cultures, IL 2 production is not augmented but rather inhibited by exogenous glutathione (GSH).
Loertscher et al. (1987)IL-2Stimulation with heated cells triggered the release of IL-1- and an IL-3-like bioactivity but did not induce IL-2 synthesis and/or release, thus explaining the lack of proliferation in the HMLR.
Ortaldo et al. (1995)IL-2NK cellsPeripheral NK cells were examined for ligand-induced death using antibodies to surface moieties (CD2, CD3, CD8, CD16, CD56), with and without prior activation of IL-2.
Manger et al. (1986)IL 2This antibody, designated J64, induced IL 2 secretion by HUT 78 in combination with PHA, T3 antibodies, or calcium ionophores, however not by itself.
Tsubata et al. (2005)interleukin 2T-cellPDZ domain-binding motif of human T-cell leukemia virus type 1 Tax oncoprotein is essential for the interleukin 2 independent growth induction of a T-cell line.
Ding et al. (2003)IL-2PBMCIn contrast, p12 expression in PBMC elicited increased IL-2 production in the presence of phorbal ester stimulation, but not during TCR stimulation.
Adams et al. (1992)IL-2HUVEInterestingly, mAb to MHC class I and class II molecules, which significantly impaired HUVE-induced proliferation, caused little interference with HUVE-induced IL-2 production.
Teng et al. (1996)IL-2By contrast, receptors which contained single tyrosine 188, 191 or 200 to phenylalanine substitutions were unable to induce IL-2.
Teng et al. (1996)IL-2These results imply that in this system phosphorylation of tyrosine 173 and hence activation of PI3-kinase is not required for CD28 induced IL-2 secretion.
Teng et al. (1996)IL-2Despite an apparent requirement for phosphorylation of all three of these tyrosines, however, receptors which contain tyrosine only at positions 191 or 200 and a truncated receptor which does not contain tyrosine 200 induce normal IL-2.
Liang et al. (1992)IL-2Flow cytometric studies showed that the level of surface IL-2 receptors was not increased by GSH treatment.
Podtschaske et al. (2007)IL-2T-cellThe stimulation of a human cytomegalovirus (CMV) specific T-cell line with CMV peptide-pulsed autologous APC also induced IL-2 expression but did not show two seperated populations.
Zubiaga et al. (1991)IL-2This is not due to posttranslational conversion of inactive NFkB into its active form by CHX, because a clustered mutation in the kB-like sequence in the IL-2 enhancer that abrogates NFkB binding does not affect the up-regulation of IL-2 transcription.
Murakami et al. (1988)IL2IL1HSFThese results indicate that HSF interferes with IL2 production and not its induction by IL1 or its interaction with the IL2 receptor.