Viewing affirmative mentions of transcription in T cells

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Fattorossi et al. (2002)MUC1T cellsHere we show that resting human T cells express basal levels of MUC1 mRNA and protein forms with molecular masses of approximately 150 and approximately 250 intracellularly, but lack surface expression.
Honda et al. (1988)PTHrPT-cellHuman parathyroid hormone-related protein (PTHrP) mRNA was detected in peripheral leukemic cells obtained from adult T-cell leukemia (ATL) patients as well as in cultured human T-cell leukemia virus type I (HTLV-I)-infected T-cell lines.
McGuire and Rothenberg (1987)IL2 geneT-cellTo study the regulation of the murine IL2 gene in T-cell populations of differing stages of maturation, we have used a calcium ionophore in conjunction with the phorbol ester, TPA, to stimulate IL2 gene transcription while bypassing the requirement for triggering through a mature cell surface receptor.
Landsverk et al. (2002)IL-2 geneT-cellThe normally repressed IL-2 gene is transcribed in nuclei from quiescent human T cells and from various non-T-cell lines.
Pahlavani and Richardson (1996)IL-2T cellsThe age-related decline in IL-2 production has been shown to arise from a decline in IL-2 transcription, and a recent study suggests that the transcription factor NFAT (nuclear factor of activated T cells) may play a role in the decline in IL-2 transcription.
Meléndez et al. (2000)ARFT cellWe have cloned and characterized a 2.5 kb region upstream of the murine p19(ARF) gene to determine the role of DNA methylation in suppressing p19(ARF) transcription in a wide panel of murine primary T cell lymphomas.
Migliaccio et al. (2005)RAG-2T cellsThese ex vivo generated T cells resemble precursors for the lymphoid lineage present in adult bone marrow in terms of active transcription of RAG-2 and pTalpha.
Dautry-Varsat et al. (1988)IL-2T-cellCsA also prevents the constitutive secretion of IL-2 in this T-cell line by blocking transcription of the IL-2 gene.
de Mestre et al. (2007)HpseT cellsFurthermore, the proximal region of the promoter, identified as important in the regulation of Hpse transcription, was shown to become accessible in T cells upon cell activation.
Ware and Haynes (1993)CD7T cellsPHA prolonged T cell CD7 mRNA stability without affecting CD7 transcription, while stimulation of PB T cells with CD3 mAb both increased T cell CD7 transcription and prolonged CD7 mRNA stability.
Ware and Haynes (1993)CD7T cellCyclosporin A inhibited ionomycin-induced T cell CD7 upregulation at the level of CD7 mRNA transcription and elongation.
Lewis et al. (2004)CD28T cellLigation of CD28 by an antibody or by CD80 also down-regulated CD28 transcription through the same mechanism, providing evidence that CD28 can generate a T cell receptor-independent signal with a unique biological outcome.
Lattier et al. (1989)ADA geneT cellsADA gene transcription occurs at higher rates in T cells than in B cells and fibroblasts.
Costerousse et al. (1993)ACET-lymphocytesAn mRNA for ACE was detected and characterized after reverse transcription and amplification by PCR in T-lymphocytes and several T-cell leukaemia cell lines.
Chen (2001)interleukin-2T cellTriptolide inhibits both Ca(2+)-dependent and Ca(2+)-independent pathways and affects T cell activation through inhibition of interleukin-2 transcription at a site different from the target of cyclosporin A.
Ikeda et al. (1993)parathyroid hormone-related peptideT cellsTranscription of the gene for parathyroid hormone-related peptide from the human is activated through a cAMP-dependent pathway by prostaglandin E1 in HTLV-I-infected T cells.
O'Riordain et al. (1993)IL-2T cellCONCLUSIONS: These results suggest that the principal cellular abnormalities that result in altered T cell activation and IL-2 production after thermal injury lie downstream of the initiating signal transduction events and before IL-2 gene transcription.
Norris et al. (2003)TCRbetaT cellsThese data demonstrate that Ebeta is active pre-thymically; however, pre-thymic transcription of the TCRbeta chain gene is neither required for T cell development, nor is it limited to pre T cells.
Fu et al. (2009)HER2/neuT cellsThe HER2/neu mRNA and protein expressions in the vector-transfected 16HBE-T cells were detected by RT-PCR and Western blot method respectively.
Fu et al. (2009)HER2/neuT cellsThe level of HER2/neu mRNA in the 16HBE-T cells transfected with pSIREN-RetroQ-neu was significantly reduced as compared to the negative control and blank control cells (0.114 +/- 0.003 vs.blank control 0.186 +/- 0.001, t = 39.154, P < 0.05; and negative control 0.182 +/- 0.015, t = 7.564, P < 0.05), while its level did not differ significantly between negative control cells and blank control of 16HBE-T (t = -0.409, P > 0.05).
Bott et al. (1994)CD6 mRNAT cellThe data suggest that the effect of PMA on CD6 expression is mediated primarily by an increase in CD6 mRNA transcription after PKC activation. mAbs were used to determine whether augmented CD6 expression could be induced by perturbation of specific T cell surface molecules.
Noh et al. (1998)IL-10T-cellsHuman IL-10 mRNA transcripts were detected in both CD3+ T-cells and CD19+ B-cells.
Whitehurst et al. (2001)TCRbetaT cellAt the T cell receptor beta chain locus (TCRbeta), two DNase I hypersensitive sites within the Jbeta2-Cbeta2 intron contained binding sites for NF-kappaB and additional nuclear factors and were postulated to be involved in controlling TCRbeta transcription and V(D)J recombination.
Whitehurst et al. (2001)TCRbetaT cellWe found that TCRbeta transcription and V(D)J recombination and T cell development were normal in these mutant mice.
Debrue et al. (2005)IL-17T-cellThe mRNA expression of IL-17 in BAL was studied using real-time polymerase chain reaction (PCR) and CD3-zeta was used as a marker of T-cell numbers.
Tsytsykova and Goldfeld (2000)TNF-alphaT cellsHere, we show that nuclear factor of activated T cells transcription factor, NFATp, controls susceptibility to superantigen-induced lethal shock in mice through its activation of TNF-alpha gene transcription.
DiSanto et al. (1990)IL-2T cellThis patient's immunological defect appears to be attributable to a selective deficiency in T cell production of IL-2, which may reflect a subtle abnormality in the IL-2 gene locus or a defect in a regulatory factor necessary for IL-2 transcription.
Villiger et al. (1991)IL-6T cellBy in situ hybridization it was shown that the cells expressing IL-6 mRNA after mitogen activation of PBMC do not belong to the T cell lineage.
Becker et al. (2007)IFN-gammaT cellsDespite constitutive retroviral IFN-gamma mRNA transcription, translation and secretion of IFN-gamma protein was tightly regulated and only observed in activated T cells.
Stranick et al. (1995)IL-5T cellCis-acting regions in the 5'-flank of the mouse interleukin 5 (IL-5) gene involved in the specific and inducible regulation of IL-5 transcription in an untransformed mouse T cell clone, D10.G4.1, have been identified.
Stranick et al. (1995)IL-5T cellProteins that bind to these elements are likely to be important inducible and specific factors essential for control of IL-5 transcription in response to T cell receptor-mediated signaling events.
Park et al. (1993)IL3T lymphocytesTranscriptional regulation of interleukin 3 (IL3) in primary human T lymphocytes.
Park et al. (1993)IL3 geneT lymphocytesUsing transfection and reporter gene assays specifically designed for primary T lymphocytes in conjunction with gel retardation assays, Western blot analyses and UV cross-linking studies, we found that c-Jun, c-Fos, and octamer-binding proteins play a major role in transcriptional activation of the IL3 gene via their interaction with two specific regions contained within the IL3 5'-flanking sequence.
Hopkins and Failla (1999)IL-2 geneT lymphocytesThese data indicate that decreased cellular Cu attenuates IL-2 synthesis in T lymphocytes by inhibiting transcription of the IL-2 gene.
Yasukawa et al. (1999)CXCR4T lymphocytesNorthern blot analysis of mRNAs extracted from HHV-6A-, HHV-6B-, and HHV-7-infected CD4+ T lymphocytes demonstrated a markedly decreased level of CXCR4 gene transcription, but the posttranscriptional stability of CXCR4 mRNA was not significantly altered.
Grumont and Gerondakis (1990)c-relT-cellsMurine c-rel transcription is rapidly induced in T-cells and fibroblasts by mitogenic agents and the phorbol ester 12-O-tetradecanoylphorbol-13-acetate.
Yeh et al. (1988)TAPT cellTAP transcription and phosphatidylinositol linkage mutants are defective in activation through the T cell receptor.
Marçais et al. (2006)CCL5T cellsCell-autonomous CCL5 transcription by memory CD8 T cells is regulated by IL-4.
Marçais et al. (2006)CCL5T cellsWe found that IL-4 treatment of murine CD8 T cells impairs immediate CCL5 secretion capacity by inhibiting CCL5 mRNA transcription through a STAT6-dependent pathway.
Chen et al. (2008)Ifi202 geneT cellSimilarly, stimulation of cells of a mouse T cell hybridoma cell line (2B4.11) also activated transcription of the Ifi202 gene.
Waldmann et al. (1985)IL-2T-cellIn human T-cell lymphotropic virus-I infected cells, the Mr 42,000 long open reading frame protein encoded in part by the pX region of this virus may act as a transacting transcriptional activator that induces IL-2 receptor gene transcription, thus providing an explanation for the constant association of IL-2 receptor expression with adult T-cell lymphotropic virus-I infection of lymphoid cells.
Horner et al. (1995)CD40LT cellsNeither CD40L surface expression nor CD40L mRNA were detected in unstimulated gamma/delta T cells.
Baumann et al. (1991)IL-2T-cellThe structurally unrelated immunosuppressive drugs cyclosporin A (Sandimmun) and FK-506 both interfere with the process of T-cell proliferation by blocking the transcription of the T-cell growth factor interleukin-2 (IL-2).
Ghasemlou et al. (2007)IL-2T cellOf the T cell cytokines assessed, there was a marked reduction in the mRNA expression of interleukin-2 (IL-2) in Nude mice compared with wildtype animals.
Melvin et al. (1995)IFN-gammaT cellsTo determine the potential role of methylation in the regulation of interferon-gamma (IFN-gamma) gene transcription by T cells, primary T-lineage cell populations were analyzed for the extent of methylation of three CpG sites within or near transcriptional activator elements in the 5' flank and first intron of the human IFN-gamma gene.
Mönning et al. (1990)APPT cellInduction of APP transcription, translation and secretion was observed with several T cell mitogens but was highest with phytohemagglutinin.
Calado et al. (2006)IL-10T cellsIn vivo Ag-experienced T cells show a higher basal probability to transcribe IL-10 when compared with naive cells, yet still show mostly monoallelic IL-10 expression.
Weill et al. (1996)TNF-alphaT lymphocytesIn this work, we present evidence that enriched human peripheral blood T lymphocytes, depleted of contaminating monocytes, rapidly express tumor necrosis factor alpha (TNF-alpha) mRNA when exposed to low doses of gamma-irradiation.
James and Kazenwadel (1989)GM-CSFT-cellStimulation of a murine T-cell line (O18A) by Con A has been shown to cause a large increase in the cytoplasmic granulocyte-macrophage colony-stimulating factor (GM-CSF) mRNA level.
James and Kazenwadel (1989)GM-CSFT-cellThis sequence is contained within a region found to be involved in regulating inducible GM-CSF transcription in a human T-cell line [Miyatake, S., Seiki, M., Yoshida, M. & Arai, K. (1988) Mol.
Flanagan and Crabtree (1992)IL-2T-cellThe activation-dependent, T-cell-specific in vitro transcription system described here should facilitate the dissection of signals that emanate from the T-cell surface resulting in IL-2 transcription and T-cell activation.
Klein et al. (1996)IL4T cellsThis shows that transcription of both IL4 forms is not restricted to T cells and can be induced in other cell types as well.
Ishiguro et al. (1995)Id2T-cellInterestingly, Id2, but not Id3, mRNA was strongly expressed in 4/5 T-cell lines infected with human T-cell leukemia virus type I (HTLV-I) (ATL-1k, MT-2, S-LB1) and type II (Mo).
Cristillo et al. (2002)CXCR4T lymphocytesHere we show that DcAMP treatment of purified human T lymphocytes increased transcription of CXCR4 mRNA as well as cell surface and intracellular CXCR4 protein expression.
Davé et al. (2004)SP-D geneT cellsHere, we identify a role of nuclear factor of activated T cells (NFATs) in regulation of murine SP-D gene (Sftpd) transcription.
Wieder et al. (1990)IL-2T-cellsTo amplify very low levels of IL-2 mRNA, sequential reverse transcription and polymerase chain reaction (RT-PCR) of T cell mRNA were used to demonstrate the capacity of the calcium signal (ionomycin) to promote low-level IL-2 transcription in normal human T-cells without additional signals.
Tara et al. (1993)IL-4T cellsTo identify cis elements that regulate IL-4 gene transcription, various amounts of the 5' flanking region of the murine IL-4 gene were linked to a chloramphenicol acetyl transferase (CAT) reporter gene and tested for the ability to modulate CAT gene transcription in PMA-stimulated EL-4 T cells.
Schraven et al. (1994)LPAP proteinT-cellAnalysis of LPAP protein and mRNA expression in CD45-deficient mutant T-cell lines suggests that LPAP protein is subjected to degradation in the absence of its binding partner, CD45.
Zhang et al. (2009)PHPT1T cellAnother example of PHPT1 being involved in a proliferation context is a recurrent 9q34 duplication in paediatric T cell acute lymphoblastic leukaemia that was found in 33% of the cases with increased mRNA expression of the PHPT1 gene (12).
Bubier et al. (2007)IL-21T cellsWhile it is clear that IL-21 is actively transcribed by naïve activated T cells, recent studies have shown that IL-21 potentially promotes a developmental shift of naïve T cells toward the Th2 phenotype.
Bamford et al. (1996)IL-15T-cellThus, IL-15 synthesis by the adult T-cell leukemia line HuT- 102 involves an increase in IL-15 mRNA transcription and translation secondary to the production of an HTLV-I R element fusion message that lacks many upstream AUGs.
Gerlo et al. (2006)PRLT lymphocytesHere we report that cAMP is an important stimulator of PRL transcription in primary human T lymphocytes.
Ferguson et al. (2001)IL-2 geneT-cellShortly after T-cell stimulation, transcription of the IL-2 gene is upregulated.
Xia et al. (2004)ghrelin receptor mRNAT cellsThe enriched T cells express the mRNA of ghrelin and ghrelin receptor mRNA, and there is a significantly positive correlation between them.
Ishiguro et al. (1995)Id3T-cellInterestingly, Id2, but not Id3, mRNA was strongly expressed in 4/5 T-cell lines infected with human T-cell leukemia virus type I (HTLV-I) (ATL-1k, MT-2, S-LB1) and type II (Mo).
Marçais et al. (2006)ccl5T cellsWe demonstrate that the CCL5 mRNA half-life is increased in memory CD8 T cells and that these cells constitutively transcribe ccl5 gene.
Marçais et al. (2006)ccl5T cellsFinally, we show that these stores are spontaneously reconstituted when the inhibitory signal is removed, indicating that the transcription of ccl5 is a default feature of memory CD8 T cells imprinted in their genetic program.
Granum et al. (2006)TSAdT cellsIn addition, TSAd mRNA expression may be induced in CD4+ T cells via cAMP dependent signals [36].
Tsujikawa et al. (2000)CD45T cellsBecause at least 8 types of CD45 isoforms can potentially be produced by alternative mRNA splicing of exons 4, 5, and 6, the analyses at the transcription and protein levels of CD45 during the development and differentiation of T cells have been performed using RT-PCR and isoform-specific monoclonal antibodies, respectively.
Dwyer et al. (2007)CD39T cellWithin the T cell compartment, the highest expression levels of CD39 transcripts were found in CD4+ cells.
Blancho et al. (1995)IL-10T cellsSince tolerance is achieved by a short course of cyclosporin A, we have also studied the in vitro effect of this drug on IL-10 gene transcription in blood mononuclear cells and have found that cyclosporin A inhibits IL-10 gene activation in T cells but does not interfere with IL-10 transcription in lipopolysaccharide-activated cells.
Ulker et al. (1990)Qa-2T cellsActivated T cells transcribe an alternatively spliced mRNA encoding a soluble form of Qa-2 antigen.
Caristi et al. (2005)interleukin-8T lymphocytesProstaglandin E2 induces interleukin-8 gene transcription by activating C/EBP homologous protein in human T lymphocytes.
Caristi et al. (2005)IL-8T cellsHere, we demonstrate that, in human T cells, PGE(2) induced IL-8 mRNA transcription through prostaglandin E(2) receptors 1- and 4-dependent signal transduction pathways leading to the activation of the transcription factor C/EBP homologous protein (CHOP), never before implicated in IL-8 transcription.
Caristi et al. (2005)IL-8T cellsOur data suggest that PGE(2) acts as a potent pro-inflammatory mediator by inducing IL-8 gene transcription in activated T cells through different signal transduction pathways leading to CHOP activation.
Sabbagh et al. (2004)caspase-3T cellThese findings indicate that the selective up-regulation of caspase-3 transcription is required to maintain the cytoplasmic levels of this protease, which control AICD and T cell homeostasis.
Gehrmann et al. (1996)FGF-2T cellBy 21 days after T cell transfer, i.e. after complete recovery, FGF-2 peptide and mRNA expression had fully subsided.
Smith et al. (2008)PTPN22T-cellEvidence of association of two SNPs in the PTPN22 gene region with Type I psoriasis susceptibility may suggest that altered levels in PTPN22 transcription may influence T-cell function and thereby influence susceptibility to psoriasis.
Saarela et al. (2006)PRKCAT lymphocytesRT-PCR revealed PRKCA mRNA to be expressed by antigen-presenting dendritic cells, by CD4+ T lymphocytes, and in a cell pool containing CD4?
Azimi et al. (1998)interleukin 15 geneT cellHuman T cell lymphotropic virus type I Tax protein trans-activates interleukin 15 gene transcription through an NF-kappaB site.
Azimi et al. (1998)IL-15T cellAdditionally, by using a Jurkat T cell transfectant that expresses Tax under an inducible promoter, we demonstrated that the expression of IL-15 mRNA increased 3-fold as Tax was expressed, suggesting that the Tax protein activates IL-15 transcription.
Deans et al. (1991)CD45T cellsDot blot analysis of mRNA from differentiating MN thymocytes indicates prolonged expression of mRNA encoding CD45 exons a, b, and c, again in contrast to peripheral T cells which lose all mRNA for alternatively spliced CD45 exons within the first 24 h poststimulation.
Martins et al. (2008)Il2T cellsPrevious studies show that IL-2 production is tightly regulated (20, 21), and that even under optimal conditions not all T cells in a population will acquire the competence to transcribe the Il2 gene and synthesize IL-2 upon primary stimulation (22).
Martins et al. (2008)Il2T cellThus, this study demonstrates that Blimp-1 represses IL-2 production after T cell activation and shows that the molecular mechanism responsible depends, at least in part, on Blimp-1–dependent repression of Il2 and Fos transcription.
Zhao et al. (2010)IL10T cellsWe further showed that treating CD4+ T cells from healthy controls with the DNMT inhibitor 5-azaC led to a significant increase in IL10 and IL13 transcription, similar to what has been shown for other SLE-related autoimmunity genes, such as CD11a [16], Perforin [17], CD70 [18], and CD40L [19].
Lloyd et al. (1997)MCP-1T-lymphocytesSignificant increases in T-lymphocytes and macrophages were observed within glomeruli and interstitium, paralleled by an induction of mRNA expression of MCP-1 and RANTES, early after disease initiation.
Arkenbout et al. (2003)TR3NOTMETHODS AND RESULTS: The mRNA expression of TR3, MINOR, and NOT in atherosclerotic lesions was assessed in human vascular specimens.
Tessier et al. (2002)interleukin-2T-cellWe assessed the implication of these PLA(2) isoforms in transcription of the interleukin-2 (IL-2) gene, involved in T-cell proliferation.
Christoforidou et al. (2004)Tpl2T-cellEvaluation of Tpl2/Cot mRNA expression in T-cell neoplasias
Georas et al. (1998)IL-4NFATNuclear factor of activated T cell (NFAT) family members play a critical role in regulating IL-4 transcription and interact with up to five sequences (termed P0 through P4) in the IL-4 promoter.