Viewing negative mentions of transcription in T cells

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Benjamin et al. (1989)IL-2T cellThe following similarities in the functional biological characteristics of T cell and B cell IL-2 suggest that B cell IL-2 is not a factor which mimics IL-2 activity in the CTLL-2 assay: (i) neutralization of IL-2 by anti-IL-2 monoclonal antibody (DMS-1); (ii) elution of IL-2 following its adsorption to CTLL-2 cells; (iii) determination of the MW of IL-2 by SDS-PAGE and Western blot analysis; and (iv) ability of B cell IL-2 to support T cell proliferation and blocking of this activity by anti-tac monoclonal antibody. cDNA probes for T cell IL-2, however, did not detect IL-2 mRNA in B cells.
Clarke et al. (1984)HLA-DR alphaT-cellWe have previously shown that two human T-cell lines (HSB and 8402) derived from patients with childhood T-cell ALL (T-ALL) do not synthesize detectable mRNA for HLA-DR alpha.
DaSilva et al. (1994)FLT3T-cellFLT3 message was not detected in five T-cell, five myeloid, four monocytic, four erythroid and five megakaryocytic cell lines.
Adachi and Rothenberg (2005)IL2T-cellsThese results identify a discrete new domain of IL2 regulatory sequence marked by dimethylated histone H3/K4 in expression-permissive T-cells even when they are not transcribing IL2, setting boundaries for histone H3 and H4 acetylation when the IL2 gene is transcriptionally activated.
Imada et al. (1996)IL-2T cellWe could not detect human T cell leukemia virus type I (HTLV-I) mRNA or interleukin 2 (IL-2) mRNA in either the tumor cells growing in mice or the original leukemic cells.
Goebels et al. (1988)IL-2 mRNAT cellIn these cell lines, IL-2 mRNA was not detectable in RNA extracted from whole adult T cell leukemia cell populations because of dilution by other RNA species from the vast majority of cells that do not contain IL-2 mRNA.
Villiger et al. (1991)IL-6T cellsWith the use of a PCR assay, IL-6 mRNA in T cells was found to be virtually negligible, and did not change after T cell activation.
Thüring et al. (1995)iNOST cellsThe lack of NO production was not due to the expression of enzymatically inactive iNOS, as we were unable to detect any iNOS protein in activated T helper clones or in freshly isolated T cells from infected mice by Western (protein) blot analysis.
Adachi and Rothenberg (2005)IL2T-cellsThese results identify a discrete new domain of IL2 regulatory sequence marked by dimethylated histone H3/K4 in expression-permissive T-cells even when they are not transcribing IL2, setting boundaries for histone H3 and H4 acetylation when the IL2 gene is transcriptionally activated.
Matheu et al. (2003)IFN-betaT cellsLack of IFN-beta also results in significantly higher antigen-specific T cells, with higher levels of IL-4, IL-5, and IL-13, whereas no significant differences in IFN-gamma response could be observed.
Mansharamani et al. (2003)BAF mRNAT lymphocytesHowever, we identified two human tissues (of 16 tested) in which BAF mRNA was not detected: thymus and peripheral blood leukocytes, which are enriched in CD4(+) T lymphocytes and macrophage precursors, respectively.
Saenz et al. (2008)PARP-1T cellT cell proliferation is impaired in the absence of PARP-1
Reimer (2010)ALKT-cellThe prognosis of PTCL is poor with the exception of the ALK (anaplastic lymphoma kinase) expressing anaplastic large cell lymphoma (ALCL) with a more favourable outcome after conventional chemotherapy and the primary cutaneous T-cell lymphomas (CTCL) that usually show an indolent clinical course [17, 18].
Joosten et al. (2010)IFNT-cellAll 4 T-cell lines had a remarkably similar phenotype, with the exception of IFN?
GeurtsvanKessel et al. (2009)IFNT cellsThe division that was induced ex vivo in CD8 T cells by IKDCs was not accompanied by vigorous IFN-?
Shankar et al. (2001)cellT cellWith the exception of B7, T cell costimulatory molecules in the rhesus have not been identified.
Dieli et al. (2000)TNF-alphaT lymphocytesThe cytotoxicity of Vgamma9 / Vdelta2 T lymphocytes was not mediated by TNF-alpha or Fas-Fas ligand, but was shown to occur through a granule-dependent mechanism that resulted in reduction of the viability of intracellular bacilli.
Dieli et al. (2000)Vdelta2T lymphocytesThe cytotoxicity of Vgamma9 / Vdelta2 T lymphocytes was not mediated by TNF-alpha or Fas-Fas ligand, but was shown to occur through a granule-dependent mechanism that resulted in reduction of the viability of intracellular bacilli.
Dieli et al. (2000)Vgamma9T lymphocytesThe cytotoxicity of Vgamma9 / Vdelta2 T lymphocytes was not mediated by TNF-alpha or Fas-Fas ligand, but was shown to occur through a granule-dependent mechanism that resulted in reduction of the viability of intracellular bacilli.
Johansson et al. (1997)miceT cellsHowever, in contrast to the findings with wild-type mice, the typical clusters of CD4+ T cells were not found in the IFN-gammaR-/- mice.
Kohyama et al. (1997)gammadelta and alphabeta IELsIELsFinally, TGF-alpha specific mRNA was not detectable in the gammadelta and alphabeta IELs even after in vitro activation.
Garcia-Sanz and Lenig (1996)interleukin (IL)-2 messenger (mRNA)T cellsIn freshly isolated T cells, calcium ionophore induces expression of interleukin (IL)-2 messenger (mRNA), but this mRNA is not translated and not loaded with ribosomes.
Fais et al. (1996)c-mycT-cellPCR analysis demonstrated that BZLF1 mRNA was virtually absent in c-myc transfectants, possibly suggesting that the deregulated c-myc imposed a block in the EBV-lytic cycle at this particular level. c-myc transfectants failed to sustain the proliferative response of autologous CD4+ T-cell clones with specificity for EBV-lytic antigens.
Kim et al. (1992)p34cdc2 proteinT lymphocytesResting murine T lymphocytes contained no detectable p34cdc2 protein, histone kinase activity, or specific mRNA for the cdc2 gene.
Ericsson et al. (1991)shared gamma/delta T cell-related superantigenT cellsThe gamma/delta T cells, induced by immunization with either W439 cells or BN spleen cells, were selective for the immunogen used and had no effect on irrelevant target cells, indicating that these effector cells were not activated by a shared gamma/delta T cell-related superantigen.
Arima et al. (1991)viral pX gene productsT cellsWe now demonstrate that HTLV-I Tax, in the absence of other viral pX gene products, is capable of inducing the nuclear expression of all four of these kappa B-binding proteins in human T cells, with most marked effects involving c-Rel and NF-kappa B p65.
Kwon and Weissman (1989)4-1BBT-lymphocyteThe other two species, 4-1BB and L2G25B, were inducible sequences found in mRNA from both a cytolytic T-lymphocyte and a helper T-lymphocyte clone and were not previously described in T-cell mRNA; these mRNA sequences encode peptides of 256 and 92 amino acids, respectively.
Benjamin et al. (1989)cellT cellThe following similarities in the functional biological characteristics of T cell and B cell IL-2 suggest that B cell IL-2 is not a factor which mimics IL-2 activity in the CTLL-2 assay: (i) neutralization of IL-2 by anti-IL-2 monoclonal antibody (DMS-1); (ii) elution of IL-2 following its adsorption to CTLL-2 cells; (iii) determination of the MW of IL-2 by SDS-PAGE and Western blot analysis; and (iv) ability of B cell IL-2 to support T cell proliferation and blocking of this activity by anti-tac monoclonal antibody. cDNA probes for T cell IL-2, however, did not detect IL-2 mRNA in B cells.
Nuss et al. (1988)HLA-DR antigensT-cellThe malignant cells were large and polymorphous; lacked terminal transferase; expressed surface CD-2, CD-3, CD-8, and HLA-DR antigens, and showed rearrangements of the T-cell-receptor beta-chain gene.
Sneller et al. (1988)VH sequencesT cellBoth the LPS and T cell induced gamma 2b mRNA transcripts lack VH sequences, implying that the surface IgG2b detected lacks a variable region.
Dukovich et al. (1987)Tac antigenT cellsIn this study, we report the identification of a second human IL-2 binding protein that (1) has an Mr of approximately 70K, (2) lacks reactivity with the anti-Tac antibody, (3) binds IL-2 with intermediate affinity and (4) is present on the surface of resting T cells, large granular lymphocytes (natural killer cells), and certain T and B cell lines in the absence of the Tac antigen.
Sterkers et al. (1987)Fc receptorsT cellThis regulation of IL-2 receptor expression by AC was specific of adherent cells, did not involve Fc receptors, was impaired when AC were metabolically inactivated and did not require T cell-AC interaction via LFA1, CD4, or HLA molecules.
Dumont et al. (1985)NZBT cellsA strain survey demonstrated the expression of the 6C3 antigen on peripheral T cells (and BM cells) of all strains examined, with the exception of NOD, NZB/B1NJ, and ST/bJ.
Dumont et al. (1985)B1NJT cellsA strain survey demonstrated the expression of the 6C3 antigen on peripheral T cells (and BM cells) of all strains examined, with the exception of NOD, NZB/B1NJ, and ST/bJ.
Ishii et al. (1982)immunoglobulinsT-cellThese studies clearly demonstrated that their malignant cells bore human Ly-l-like antigen but lacked human TL-like and Ia-like antigens as well as surface-bound immunoglobulins, indicating their peripheral T-cell origin.
Ishii et al. (1982)Ia-like antigensT-cellThese studies clearly demonstrated that their malignant cells bore human Ly-l-like antigen but lacked human TL-like and Ia-like antigens as well as surface-bound immunoglobulins, indicating their peripheral T-cell origin.
Reinherz et al. (1980)T muT lymphocytesThe results showed that the T mu population contained both inducer (OKT4+) and cytotoxic/suppressor (OKT5+) populations and was similar to the unfractionated T cell population, whereas the T gamma subset contained few T lymphocytes (OKT3+) and was not enriched for either T cell subset defined by these monoclonal antibodies.
Wollman et al. (1980)Dr antigenT cellsThis was shown to be due to the lack of Dr antigen on the stimulating cell: if allogeneically activated T cells were used as stimulating lymphocytes, a DR-specific proliferative response appeared.
Boussiotis et al. (1997)interleukin-2T cellsIn the absence of costimulation, T cells activated through their antigen receptor become unresponsive (anergic) and do not transcribe the gene encoding interleukin-2 (IL-2) when restimulated with antigen.
Maruyama et al. (1999)TCRT cellsThese CD3intCD4lowNK1.1+ T cells mediated self-reactivity and appeared even in the periphery. mRNA of an invariant chain of TCR Valpha14Jalpha281 gene product was detected in these CD4low T cells, but not remaining CD4high T cells.
Stingl et al. (1987)IL-2T cellsAnti-CD3-MoAb, in the absence of IL-2, induced IL-2 receptor expression on purified T cells, and anti-IL 2 receptor antibodies inhibited T cell proliferation in the presence of this growth factor.
Zitron et al. (1996)IFN-gammaT cellsIn contrast, only trace IFN-gamma mRNA was detected in day 30 activated SpC, and no IFN-gamma mRNA was present in purified, nonactivated T cells obtained at either time.
Priceputu et al. (2007)NefT cellsAll these Nef alleles downregulated cell surface CD4 in human cells in vitro and also, with the exception of Nef(YU10x), in Tg CD4(+) T cells.
Knoechel et al. (2005)IL-2T cellIn the absence of IL-2, the acute disease is mild because of reduced T cell effector function, but a chronic and progressive disease develops late and is associated with a failure to generate FoxP3(+) regulatory T (T reg) cells in the periphery.
Jokhi et al. (1994)LIFT cellsIn contrast, peripheral blood CD56brightCD16- NK cells, CD56dimCD16+ NK cells, and CD3+ T cells expressed mRNA only for TNF-alpha and TGF-beta 1, but not for GM-CSF, CSF-1, IFN-gamma, LIF, or IL-2.
Bianchi et al. (1996)uPART lymphocytesReceptors for urokinase plasminogen activator (uPAR) were not expressed in resting T lymphocytes, but could be efficiently induced at the mRNA and protein level by coclustering of the antigen receptor complex and beta1 or beta2 integrins, through a signalling pathway involving both protein kinase C activation and an increase in intracellular cyclic AMP.
Kwon et al. (1998)cdk2T-cellsAlthough human cdk2 genomic DNA contained the sequence of the insert for the beta form, cdk2 beta was not detected by either Western blot or RT-PCR in human T-cells or several other human cell lines.
Halle et al. (1997)LEF1T cellsWith the exception of LEF1, these factors activate transcription in T cells.
Li et al. (1994)RelAT cellsWestern blotting analysis did not detect any RelA in nuclear extracts either before or after stimulation of T cells.
Wu et al. (1990)CD4-CD8T cellsHowever, the demethylation pattern is distinct from that seen on typical peripheral T cells or on mature thymocytes, suggesting that the TCR+ CD4-CD8- thymocytes are not derived from mature thymocytes or peripheral T cells which have returned to the thymus and downregulated CD8 expression.
Moreira et al. (2008)CCR5T cellsIn the absence of CCR5, the percentage of CD4+CD25+ T cells expressing Foxp3, glucocorticoid-induced TNFR (GITR), CD103, CD45low, and CTLA-4 in the granulomas was significantly decreased.
Wu et al. (1990)TCRT cellsHowever, the demethylation pattern is distinct from that seen on typical peripheral T cells or on mature thymocytes, suggesting that the TCR+ CD4-CD8- thymocytes are not derived from mature thymocytes or peripheral T cells which have returned to the thymus and downregulated CD8 expression.
Shankar et al. (2001)B7T cellWith the exception of B7, T cell costimulatory molecules in the rhesus have not been identified.
Hughes and Pober (1993)c-fos mRNAT cellsIn contrast, c-fos mRNA is absent from resting T cells and is induced on PHA activation.
Malyguine et al. (1998)calnexinT-cellThe NK-resistant cell line NKR was originally derived from the NK-sensitive, human T-cell line CEM and does not synthesize calnexin protein or mRNA.
Rezai et al. (1999)FasLT-cellThe role of Fas ligand(FasL)/Fas-mediated T-cell suppression was excluded, since FasL protein or mRNA could not be detected on HFRPE cells with flow cytometry and by reverse transcriptase polymerase chain reaction, respectively.
Dokter et al. (1994)IL-4T cellsIn accordance with the mRNA results, IL-4 protein was not detected in supernatants of unstimulated T cells or T cells exposed to IL-7.
Wandroo et al. (2008)ZAP-70T cellsZAP-70 expression was not detected in mature B cell lines but was detected in pre-B cell lines at a level comparable to that seen in T cells.
Motokura et al. (1988)PTHrPT cellWe report here that PTHrP mRNAs were detected in a HTLV-I-infected T cell line, MT-2, but not in uninfected T cell or B cell lines, and that PTH-like bioactivity was detected only in the conditioned medium of MT-2 cells.
Miller (1996)delta opioid receptorT cellsSimilar results were obtained in a single isolation of CD8+ T cells from Con A-stimulated splenocytes. mRNA for the delta opioid receptor was not detected in CD4+ T cells purified from either freshly isolated splenocytes or splenocytes cultured without Con A, and incubation of purified CD4+ T cells with Con A did not induce delta opioid receptor mRNA.
Hentati et al. (1994)P-IgGT cellFlow cytometric analysis of various T cell subsets showed that the number of cells expressing gamma delta T cell receptor (TcR) antigens which did not vary with age was not modified after P-IgG or P-IgM treatment.
Arase et al. (1995)FasT cellsFas ligand mRNA was expressed in freshly isolated NK cells but not in T cells.
Kano et al. (2005)CD20T-cellCanine CD20 mRNA was detected in PBMCs and lymph node from healthy dogs, and B-cells of canine lymphoma, but not in T-cell lymphoma cells and non-T and non-B-cell lymphoma cells by RT-PCR analysis.
Holst et al. (2007)CCR5T-cellIn contrast, the T-cell response is accelerated in CCR5(-/-) and CCR5/CXCR3(-/-) mice and the absence of CCR5 is associated with the induction of CD8(+) T-cell-mediated immunopathology consisting of marked hepatic microvesicular steatosis.
Martin et al. (1987)preproenkephalinT-cellA B-cell lymphoma was negative for preproenkephalin mRNA, but several T-cell lymphomas were positive.
Bosque et al. (2007)IL-2T cellIn the absence of IL-2, human CD4(+) T cell blasts were sensitive to both FasL and Apo2L/TRAIL, but human CD8(+) T cell blasts died, with no additional effect of death receptor ligation.
Kim et al. (1997)HsMCAKT lymphocytesUnstimulated T lymphocytes contained no detectable HsMCAK-specific mRNA.
Ndiaye et al. (2008)OXTRT cellEach of the major T cell subsets (CD4+, CD8+, and gamma delta+) expressed OXTR mRNA, with no significant difference in expression among them.
Kersten et al. (2005)BMP-6T cellsInterestingly, in a separate study we have found that normal human T cells do not express BMP-6 mRNA after activation (Sivertsen et al, manuscript in preparation).
Chang and Aune (2005)Ifng geneT cellsTo distinguish between these, we compared long-range histone acetylation patterns across the Ifng gene region in natural killer (NK) cells and T cells that were or were not actively transcribing the Ifng gene.
Narita et al. (2010)merT cellsAlthough WT1/MHC tetramer+CD8+ T cells were not detected in PB before the WT1 peptide vaccination, WT1/MHC tetramer+CD8+ T cells appeared after the second vaccination.
Malcovati et al. (2009)MPLT lymphocytesJAK2 and MPL mutations were detected in circulating granulocytes and bone marrow CD34+ cells, but not in T lymphocytes, from 11 of 19 patients with RARS-T.
Atayar et al. (2006)IL4T cellsIn contrast to the CD4(+)/CD57(+) T cells from tonsils, IL2 and IL4 mRNAs were consistently absent from the CD4(+)/CD57(+) T cells of NLPHL.
Nordström et al. (2005)Foxp3T cellsThe blood-derived CD25+ CD8+ T cells did not express Foxp3 mRNA but had a bona fide antiproliferative capacity in response to both uninfected and HSV-2-infected DC, whereas the CD25-CD8+ T cells were selectively activated to become antiproliferative by HSV-2-infected DC.
Chen et al. (2004)foxP3T cellsCD4(+) T cells in these mice expressed higher levels of CTLA-4 and glucocorticoid-induced TNF-related receptor than naive CD4(+) T cells, but only 3% of the recolonizing cells were CD25(+) and did not express significant foxP3 mRNA.
Krupa et al. (2002)p40T-cellDC/T-cell interaction does not involve interleukin-12; transcripts for interleukin-12 p40 are absent in the vasculitic infiltrates.
Gasper et al. (2002)CXCR3T lymphocytesThe fact that osteoblasts did not express CXCR3 mRNA, whereas T lymphocytes can express high levels of this receptor, suggests that osteoblast-derived CXCL10 may recruit T lymphocytes to the sites of bone infections.
Malek et al. (2002)CD25T cellsHere, we show that CD4(+)CD25(+) regulatory T cells were not readily detected in IL-2Rbeta(-/-) mice, but the production of functional CD4(+)CD25(+) T cells was reconstituted in Tg -/- mice.
Jäger et al. (2000)SK3T cellsIn addition, we used the polymerase chain reaction to demonstrate the presence of SK2 mRNA in Jurkat T cells, whereas SK3 transcripts encoding the other cloned apamin-sensitive SK channel were not detected.
Leblond et al. (1997)IL-10T cellsReverse transcription-polymerase chain reaction assays showed similar amounts of interferon-gamma transcripts in the two subsets; tumor necrosis factor-alpha, IL-4, and IL-10 transcripts were detected in CD4+CD7- T cells but not in their CD4+CD7+ counterparts.
Möhle et al. (1997)CD2T lymphocytesT lymphocytes were not present in the endothelial cultures as demonstrated by absence of CD2 mRNA.
Brown et al. (1994)bovine T-cellThis study reports that bovine IL-10 mRNA is expressed by Th0, Th1, and Th2 clones of bovine T cells specific for either Babesia bovis or Fasciola hepatica but not by two CD8+ T-cell clones.
Marine and Winoto (1991)Gata3T-cellNorthern blot analysis showed that the Gata3 mRNA is present in T-cell, but not B-cell, macrophage or HeLa cell lines.
Lew et al. (1986)V gammaT cellsAnalysis of the glycosylation pattern of this thymic gamma chain revealed that the major variable region gamma (V gamma) gene transcribed in activated peripheral T cells is absent from this subpopulation.
Tejada-Simon et al. (2001)MBPT cellWe identified a number of dominant T cell epitopes within MBP and PLP, some of which were not previously detected using whole myelin antigens as the primary stimuli.
Brouwer et al. (2004)L-CCRT cellsIt is shown that L-CCR mRNA is expressed in infiltrating macrophages during EAE, but not in infiltrating T cells.
Fan et al. (1993)IL-2 mRNAT cellsElevated levels of IFN-gamma mRNA and lowered IL-2 mRNA were found in the PBMC of eight seropositive men with CD4 T cells over 500/mm3 (mean, 647/mm3), whereas IL-4 and IL-10 mRNA were not changed significantly.
Mori et al. (1996)IL-10T-cellFresh leukemic cells from ATL patients as well as HTLV-I-infected T-cell lines MT-2, SLB-1, and C10/MJ expressed IL-10 mRNA by reverse transcription-polymerase chain reaction analysis, whereas IL-10 mRNA was not detected in normal peripheral mononuclear cells and an uninfected T-cell line Jurkat.
Haqqi et al. (1995)TCR VT-cellsOur results indicate that (a) in H-2q mice with CIA there is a restricted usage of TCR V beta chain genes regardless of the TCR V beta genotype; and (b) in the absence of TCR V beta 8 and 9, TCR V beta 3 and 10 are predominantly used by joint-infiltrating T-cells.
Jokhi et al. (1994)CSFT cellsIn contrast, peripheral blood CD56brightCD16- NK cells, CD56dimCD16+ NK cells, and CD3+ T cells expressed mRNA only for TNF-alpha and TGF-beta 1, but not for GM-CSF, CSF-1, IFN-gamma, LIF, or IL-2.
Jäger et al. (2000)CD8T cellCD8(+) T cell responses to HLA-A2-restricted NY-ESO-1 peptides were detected in 10 of 11 patients with NY-ESO-1 antibody, but not in patients lacking antibody or in patients with NY-ESO-1-negative tumors.
Narita et al. (2010)WT1T cellsAlthough WT1/MHC tetramer+CD8+ T cells were not detected in PB before the WT1 peptide vaccination, WT1/MHC tetramer+CD8+ T cells appeared after the second vaccination.
Smyth et al. (1991)IL-8T cellsT cells stimulated with PHA, ionomycin, or PMA alone failed to express IL-8 mRNA.
Beyer et al. (2005)CD11cT cellsContamination by CD11c+ non-T cells cannot be excluded completely but is very unlikely to account for CD11c mRNA detection in CD8 T cells since no major difference in CD11c mRNA quantity was detected between CD8+ and CD11c+ cell populations.
Ley et al. (1994)fynT cellsSurprisingly, fyn was not detected at the plasma membrane in either Jurkat T cells or T lymphoblasts but was closely associated with the centrosome and to microtubule bundles radiating from the centrosome.
Curreli et al. (2001)IDOT cellsWe demonstrate here that IDO mRNA is not present in resting CD4(+) T cells.
Moss et al. (2009)NCCRP-1T-cellNCC were 3 microm, NCAMP-1(+) and NCCRP-1(+) and did not express B- and T-cell specific mRNA.