Viewing negative mentions of localization in T cells

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Buschman and Skamene (1988)IL-2T cellsThe susceptible animals were found to be T cell-unresponsive since they lacked both proliferative and IL-2 secreting specific T cells.
Lyons et al. (1986)IL 1T cellWe have confirmed that AM secrete relatively little interleukin 1 (IL 1), but addition of exogenous IL 1 did not improve the capacity of AM to initiate antigen-induced T cell proliferation.
Bogunia-Kubik et al. (2003)IL-2T cellsStimulation of T cells in the presence of CB sera increased the frequency of IL-2 producing cells (p < 0.005) (but not the amount of IL-2 secreted) and resulted in a higher expression of CD25 (p < 0.05).
Tokura et al. (2002)IL-8T cellsDepletion of CD4 but not CD8 T cells from PBMC abrogated this augmented IL-8 elaboration, and CD4 T cells per se secreted no substantial amount of IL-8 even upon CD3/CD28 stimulation.
Ebner et al. (1987)TFECT-cellT-cell unresponsiveness could not be explained by inhibitory activity of TFEC, released either into the culture supernatant or exerted by cell contact.
El-Sabban et al. (2002)VEGFT cellsWe show that HTLV-I-transformed T cells, but not HTLV-I-negative CD4(+) T cells, secrete biologically active forms of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) and, accordingly, induce angiogenesis in vitro.
Goeken et al. (1986)IL-1T cellsThey do not secrete IL-1 nor stimulate secretion of IL-2 by T cells.
Stein and Stadecker (1987)IL-1T cellsThis was based on the observations that M.5 cells failed to secrete significant IL-1 either spontaneously, or in the presence of various stimuli, and that murine recombinant IL-1 failed to substitute for PMA in the activation of T cells.
Sileghem and Flynn (1992)IL 2T cellLymph node cells from infected cattle were unable to secrete interleukin 2 (IL 2) in vitro following mitogenic stimulation and the exogenous supply of IL 2 did not restore T cell proliferative responses.
Kushnir et al. (2001)IgAT cellsWe also observed that transferred CD4+ T cells alone are capable of resolving RV shedding, although no IgA is secreted.
Krenger et al. (1996)IFN-gammaT-cellWe demonstrate that the transplantation of polarized type 2 murine T cells (i.e., cells secreting IL-4 but not IFN-gamma) together with T-cell-depleted bone marrow results in a significant increase in survival (P<0.001) after bone marrow transplantation across minor histocompatibility barriers (B10.BR-->CBA/J).
Palma et al. (2010)IFNT cellsPurified CD8+ T cells of both naïve and Ag85B-immunized mice or cells used as feeder did not release detectable amounts of IFN-?
Wang et al. (2009)IFNT cellsResponder T cells did not release IFN-?
Saïdi et al. (2009)eotaxinT cellsConversely to M(BL) and M(BL-X4), M(BL-R5) did not secrete eotaxin, GRO, ITAC, lymphotactin, MIP-1, MIP-3, and RANTES, which was associated with a weak capacity to recruit CD4(+)CXCR4(+)CCR5(+) T cells.
Saïdi et al. (2009)GROT cellsConversely to M(BL) and M(BL-X4), M(BL-R5) did not secrete eotaxin, GRO, ITAC, lymphotactin, MIP-1, MIP-3, and RANTES, which was associated with a weak capacity to recruit CD4(+)CXCR4(+)CCR5(+) T cells.
Hamzeh-Cognasse et al. (2008)IL-1betaT cellIn addition, we observed that DCs co-cultured with PLTs in filter-separated compartments acquired a mature phenotype (high CD80, CD86, and intermediate CD83 expression; IL-12(p70) production; efficient stimulation of autologous CD4+ T cell proliferation), while DCs co-cultured with PLTs in the same compartment did not undergo phenotypic maturation, did not secrete IL-12(p70) or IL-1beta, but instead induced moderate Th2-polarized T cell proliferation.
Adhikary et al. (2007)Th1 cytokinesT cellUpon target cell recognition all LCL-reactive T cell lines predominantly secreted Th1 cytokines (Fig. 1E), except for the T cell line derived from IM7.
Bendle et al. (2007)IFNT cellsrevealed that tolerant T cells were unable to secrete IFN-?
Wang et al. (2007)NFATNFATFollowing coculture with K562 targets, CSA-exposed NK cells differed from controls and lacked Ca(2+) oscillations, nuclear factor of activated T cells (NFAT) dephosphorylation, and NFAT nuclear translocation.
Pennington et al. (2006)interferon-gammaT-cellHere we show that without any obvious effect on TCR-mediated selection, the normal differentiation of mouse gammabeta T cells into potent cytolytic and interferon-gamma-secreting effector cells is switched towards an aggregate regulatory phenotype by limiting the capacity of CD4+CD8+ T-cell progenitors to influence in trans early gammabeta cell progenitors.
Biagi et al. (2005)granzyme-BT cellsThe culture of CD40L/OX40L-expressing B-CLL cells with autologous T cells generated CD4+/CD8+ cytotoxic T-cell lines, which secreted interferon-gamma (IFN-gamma) and granzyme-B/perforin in response to autologous, but not to allogeneic, B-CLL cells or to autologous T-cell blasts.
Biagi et al. (2005)perforinT cellsThe culture of CD40L/OX40L-expressing B-CLL cells with autologous T cells generated CD4+/CD8+ cytotoxic T-cell lines, which secreted interferon-gamma (IFN-gamma) and granzyme-B/perforin in response to autologous, but not to allogeneic, B-CLL cells or to autologous T-cell blasts.
Voo et al. (2005)interleukin (IL)-10T cellsCD4(+) Treg cells do not secrete interleukin (IL)-10 and transforming growth factor beta cytokines but express CD25, the glucocorticoid-induced tumor necrosis factor receptor-related protein (GITR), and Forkhead Box P3 (Foxp3), and are capable of suppressing the proliferative responses of naive CD4(+) and CD8(+) T cells to stimulation with mitogenic anti-CD3 antibody.
Molloy et al. (2005)cellT cellLike antibodies, T cell receptors are produced with huge diversity but, unlike antibodies, T cell receptors are not secreted and do not undergo somatic mutations that increase their affinities for antigen.
Lichterfeld et al. (2004)tumor necrosis factor-alphaT cellsThese experiments revealed considerable intraindividual and interindividual differences among epitope-specific T-cell effector functions: while the frequency of HIV-1-specific CD8(+) T cells secreting interferon-gamma but no tumor necrosis factor-alpha (TNF-alpha) following antigenic stimulation was only weakly correlated to their cytotoxic activity (R = 0.05, P =.57), a subset of CD8(+) T cells secreting both inter-feron-gamma and TNF-alpha was substantially more strongly associated with cytotoxicity (R = 0.67, P <.001).
Verjans et al. (2004)interleukin (IL)-8T cellThe T cell clones secreted interferon- gamma, tumor necrosis factor- alpha, interleukin (IL)-8, macrophage inflammatory protein-1 alpha, and RANTES (regulated on activation, normally T cell expressed or secreted), but they secreted no or limited IL-4.
Appleman and Boussiotis (2003)interleukin (IL)-2T cellsAnergic T cells do not proliferate or secrete interleukin (IL)-2 in response to appropriate antigenic stimulation.
Wizel et al. (2002)TNF-alphaT cellCD8(+) T cell lines to the 18 Cpn epitope-bearing peptides were cytotoxic, displayed a memory phenotype, and secreted IFN-gamma and TNF-alpha, but not IL-4.
Yamashiro et al. (2002)IFN-gammaT cellsProductively stimulated nai;ve T cells expressed IL-2 to differentiate into T helper 1 (Th1) cells, secreting interferon-gamma (IFN-gamma) upon secondary antigen stimulation; T cells primed with an APL did not secrete either interleukin-4 (IL-4) or IFN-gamma, but expressed TGF-beta1 and Tob, a member of the anti-proliferative gene family.
Soussi et al. (2000)IFN-gammaT cellsThese primed IFN-gamma-secreting LACK-reactive T cells were not detected ex vivo after day 7 of immunization but could be recruited and detected 15 days later in the draining lymph node after an L. major footpad challenge.
Toda et al. (2000)interleukin (IL)-4T-cellSplenic T cells isolated from mice inoculated with pCACJ1 i.m. secreted interferon-gamma (IFN-gamma), but not interleukin (IL)-4, in vitro upon stimulation with Cry j 1 as well as with p277-288, a peptide corresponding to the T-cell epitope of Cry j 1.
Toda et al. (2000)interferon-gammaT-cellSplenic T cells isolated from mice inoculated with pCACJ1 i.m. secreted interferon-gamma (IFN-gamma), but not interleukin (IL)-4, in vitro upon stimulation with Cry j 1 as well as with p277-288, a peptide corresponding to the T-cell epitope of Cry j 1.
Sireci et al. (1999)IFN-gammaT cellsIn this study we report that a single, subcutaneous injection of the peptide emulsified in IFA gave rise to the development of male-specific CD8+ T cells which displayed H-Y-specific proliferative response in vitro and showed a Tc1-type pattern of cytokine production (i.e. they secreted IFN-gamma and IL-2, but not IL-4 and IL-10).
Badovinac et al. (1998)IFN-gammaT-cellAnalysis by reverse transcription-polymerase chain reaction revealed that, in contrast to mouse, rat NK T cells secrete exclusively IFN-gamma and not IL-4 after anti-CD3 stimulation, and use a wider TCR-Vbeta repertoire, suggesting that rat NK T cells are not essential for the development of Th2-type CD4+ T-cell responses.
Ryan et al. (1997)IFN-gT cellsIn an investigation of cell-mediated immunity against Bordetella pertussis, we found that B. pertussis infection in infants and in mice was associated with the induction of antigen-specific T cells that secrete IFN-g and IL-2, but not IL-4 or IL-5.
Jonuleit et al. (1997)IFNT cellsDC cultured with TNF-alpha/IL-1/IL-6 or MCM alone induced CD4+ T cells that release intermediate levels of interferon (IFN)-gamma and no IL-4 or IL-10.
Murch et al. (1996)TNFT cellAt all ages from birth, lavage supernatants demonstrated highly significant increase over controls of the beta-chemokine macrophage inflammatory protein (MIP)-1 alpha, although not of regulated upon activation, normal T cell expressed and secreted (RANTES), of the cytokines tumor necrosis factor (TNF)-alpha and IL-1 beta, and of elastase/alpha-1 antitrypsin.
Fuleihan et al. (1991)IgT cellsFinally, in contrast to its capacity to promote mitogen-trigered B cell proliferation, TSST-1 failed to induce the differentiation of B lymphocytes into Ig-secreting cells in the absence of added T cells.
Davidson et al. (1991)Ly-5T cellsIn contrast to normal CD4+ T cells, lpr and gld CD4+ Ly-5(B220)+ T cells proliferated weakly and did not secrete TNF-alpha, IL-2, or, in most experiments, IFN-gamma after stimulation.
Flynn et al. (1989)sheep T cellCharacterisation of the cloned cell line showed that it did not secrete sheep immunoglobulin (Ig) molecules, express Ig on the surface membrane, or express normal sheep B or T cell surface markers.
Sarfati et al. (1985)IgAT lymphocytesHence, it is concluded that upon incubation with S-IgA, but not with another Ig class, T lymphocytes release IgA-specific suppressor factors.
Viac et al. (1977)IgT lymphocytesIn weakly malignant tumours, the infiltrate is characterized by an elevated number of T lymphocytes and numerous plasma cells which secrete all classes of Ig; in highly malignant tumours it is characterized by a reduced number of both T lymphocytes (E rosette) and plasma cells which do not secrete all classes of Ig.
Mendel and Shevach (2002)IL-10T cellsWe were not successful in our attempts to generate significant numbers of antigen-specific T cells that secreted IL-10, but not IL-4, by culture in the presence of IL-10.
Kitajima et al. (1995)IL-1 betaT cellsIncubation with HDK-1 cells alone or with KLH alone caused no IL-1 beta secretion, indicating the requirement for both T cells and Ag.
Hosking et al. (2009)CXCR2T cellHowever, our findings clearly indicate that in the absence of CXCR2 signaling there are no deficiencies in either the frequencies or numbers of JHMV – specific T cells or T cell activation markers, regardless of whether mice were challenged within the CNS or peripherally.
Lichterfeld et al. (2004)CD8T cellsThese experiments revealed considerable intraindividual and interindividual differences among epitope-specific T-cell effector functions: while the frequency of HIV-1-specific CD8(+) T cells secreting interferon-gamma but no tumor necrosis factor-alpha (TNF-alpha) following antigenic stimulation was only weakly correlated to their cytotoxic activity (R = 0.05, P =.57), a subset of CD8(+) T cells secreting both inter-feron-gamma and TNF-alpha was substantially more strongly associated with cytotoxicity (R = 0.67, P <.001).
Nagasawa et al. (2005)sCD40LT cellsThese results indicate that sCD40L in vivo is released mainly from the platelets or in the process of platelet production but not from the activated T cells.
Andrew et al. (1984)IFN-gammaT lymphocytesIt is evident that IFN-gamma is not the only macrophage activator released by T lymphocytes responding to microbial antigen, and may not even be the main one to enhance antimicrobial activity in infections such as tuberculosis.
Lech et al. (2008)SigirrT cellIn the absence of Sigirr, CD4 T cell numbers were increased and CD4+CD25+ T cell numbers were reduced.
Fauquembergue et al. (2010)CAP1T-cellAltogether, our data indicate that CAP1 is not efficiently processed and presented by CEA+ tumor cells, and therefore, is not an appropriate target for T-cell-based immunotherapy.
Valenzuela et al. (2009)PKCthetaT cellFurthermore, absence of PKCtheta raised the threshold for T cell activation, which selectively affected alloresponses.
Sakata et al. (1998)FasT cellsThe soluble form of CH-11 and other immobilized anti-Fas mAb (UB-2, ZB-4; IgG type) failed to stimulate lupus T cells while immobilized human Fas ligand did.
Thomis et al. (1995)IL-2T cellsIn response to mitogenic signals, peripheral T cells in Jak3-deficient mice did not proliferate and secreted small amounts of IL-2.
Prince et al. (2009)ItkT-cellIn the absence of Itk and Rlk, T-cell receptor signaling is impaired, with defects in mitogen-activated protein kinase activation, Ca(2+) mobilization, and actin polymerization.
Thude et al. (1995)CD45RAT cellsCD45RA isoforms (containing exon A-encoded sequences) can usually be found on a subset of resting T cells, but not on activated T cells.
Gloria-Bottini et al. (2008)ADA1T cellRecent data suggest that ADA1 and CD26 are co-localized on the T cell surface but not inside cells.
Ochanuna et al. (2010)TGFT cellsSpecifically, Sauer et al have shown that that pre-mature termination of TCR triggering, either by TCR signal withdrawal or specifically by PI3K inhibitor significantly increased FoxP3 expression by naïve T cells, in the absence of TGF-?
Gideon et al. (2010)CD4T cellsT cell phenotypes were defined based on the surface markers CD45RA and CD27: Central memory cells (TCM) are positive for CD27 and negative for CD45RA; effector memory (TEM) are negative for both CD27 and CD45RA and Terminally differentiated T cells (Tdiff) are negative for CD27 and positive for CD45RA.
Casazza et al. (2009)Brefeldin AT cellsThis supposition is supported by data showing down regulation of CCR5 expression on memory CD4+ T cells stimulated by anti-CD2, CD3 CD8 activation beads in the absence of Brefeldin A; the reversal of this affect by anti-MIP-1?
Monti et al. (2008)rapamcyinT-celleffector T-cells in the absence of rapamcyin leads to normal T-cell proliferation (Fig. 2B; 20).
de Beaucoudrey et al. (2008)TGFT cellsmay have no effect or may even inhibit the development of human IL-17–producing T cells (5–8) was neither supported nor disproved by our data for patients with mildly enhanced TGF-?
Schweizer et al. (2008)DARPinsT cellsAll probed selected DARPins bound to CD4+ cell lines and to primary CD4+ T cells but not to CD4?
Jeeninga et al. (2008)TNFT cellsproduction by monocytes, which are still present in the T cells preparations [49], could explain the lack of a TNF?
Soros and Greene (2006)massT cellsUnfortunately, this barrier is forfeited when CD4 T cells are activated because A3G is recruited into inactive high molecular mass ribonucleoprotein complexes.
Shim et al. (2002)caspase-9T cellTMC specifically blocked tyrosine phosphorylation of intracellular signal mediators downstream of Src tyrosine kinases in a T cell-specific manner, leading to apoptosis due to cleavage of Bcl-2, caspase-9, caspase-3, and poly(ADP-ribose) polymerase, but not caspase-1.
Lu et al. (2001)TGF-betaT cellsT cells stimulated by liver-derived DEC205+B220+D19- cells release both IL-10 and IFN-gamma, small amounts of TGF-beta, and no IL-2 or IL-4, a cytokine profile resembling T regulatory type 1 cells.
Raz and Spiegelberg (1999)IgG2aT cellsIn contrast, mice immunized with pCMV-LacZ formed predominantly IgG2a antibodies and their CD4+ T cells secreted IFN-gamma but no IL-4 and IL-5.
Winter et al. (1999)IFN-gammaT cellsTherapeutic T cells from wt, PKO, or gld mice exhibit a tumor-specific type 1 cytokine profile; they secrete IFN-gamma, but not IL-4.
Holoshitz et al. (1993)MHCT cellsClass II MHC molecules were neither sufficient nor necessary for effective presentation of AP-MT to the gamma delta T cells, as transfectants expressing class II MHC molecules were unable to present, whereas cell lines lacking expression of MHC class II molecules could present this mycobacterial antigen.
Ogata et al. (1989)IFNsT cellWhen these parental lines and its clones were tested for their ability to produce various types of cytokines, it was revealed that they were capable of producing colony-stimulating factor (CSF), IL6, and thymic stroma-derived T cell growth factor (TSTGF), which was recently described, but were unable to generate other lymphokines and IFNs.
Yuan et al. (1985)gamma 1 chainsT cellOur results show that neither nascent mRNA for gamma 1 chains nor mIgG1 can be detected in B lymphocytes until they have been stimulated by both LPS and BCDF gamma-containing T cell SN, and suggest that cell surface expression and secretion of IgG1 are coordinately controlled.
Rock and Benacerraf (1984)GATT cellNevertheless, the inhibitory effect of GAT was localized to the stimulator cell and not to the T cell hybrids.
de Baetselier et al. (1980)anti-Ig-coated plastic platesT cellsThis response was found to be mediated by T cells, since it could be obtained with nylon-wool passed cells and cells which do not adhere to anti-Ig-coated plastic plates.
Thomas et al. (1990)cell GM-CSFT cellT cell GM-CSF secretion in response to PHA is also reduced or absent.
Smith (2004)IL2T cellsHowever, upon subsequent and more extensive testing of IL2 (-/-) mice, it was found that in the absence of IL2, the marked proliferative expansion of LCMV-induced CD8+ T cells was virtually eliminated, the total cytolytic effector capacity was reduced by > 90%, and IFN-?
Pinkston et al. (1983)interleukin-2T cellsBlood T cells from the same patients did not release interleukin-2.
Miyazaki and Osawa (1983)IL 1T cellsThe antigen-presenting functions of DC and M phi were compared by measuring the capacity of KLH-pulsed, UV-irradiated cells, which failed to secrete interleukin 1 (IL 1), to stimulate KLH-immune T cells when each culture was supplemented with an equal amount of exogenous IL 1.
Prince et al. (2009)RlkT-cellIn the absence of Itk and Rlk, T-cell receptor signaling is impaired, with defects in mitogen-activated protein kinase activation, Ca(2+) mobilization, and actin polymerization.
Zhan et al. (2009)SOCS1T cellsIncrease in CD25+CD4+ T cells in the absence of SOCS1 could be partly due to enhanced survival by CD25+CD4+cells, to a lesser degree CD25-CD4+ T cells, from SOCS1-deficient mice with or without T-cell growth factors.
Tingjun and Rongliang (2004)IL-2T cellsThe results showed that SSP1 activated T cells to release NO and secrete IL-2 by modulating activity of PKC and level of intracellular free calcium.
Socarras et al. (1993)IL-4T cellsSpleens of adult mice of the A strain background that were rendered tolerant as neonates of class II alloantigens only (A.TH tolerant of A.TL, A.TL tolerant of A.TH) contain large numbers of tolerogen-responsive T cells, many of which secrete IL-4, but not IL-2.
Yang et al. (2003)TRAILT cellsT cells treated with Tat did not secrete enough TRAIL to mediate cell death in our assay.
Piacibello et al. (1986)HuIFN gammaT lymphocytesT lymphocytes did not release these activities in the absence of PHA with or without HuIFN gamma.
Petitjean et al. (2007)CD4T lymphocytesIn sustained responders to HAART, resting CD4+ T lymphocytes do not spontaneously release HIV-1 [16,17].
Dilloo et al. (1996)IL-3T cellIL-3 protein release is regulated via the CD2 receptor with virtually no IL-3 release after T cell stimulation via CD3.
Barros Kanzaki and Casseb (2007)T-cell lymphotropic virus type IIT-cellHuman T-cell lymphotropic virus type II is a retrovirus endemic in Amerindian communities throughout the American continent, although some Amerindian groups that apparently emerged from the same ethnic root as HTLV-II carriers do not secrete antibodies against the virus and show very low prevalence for human T-cell lymphotropic virus type I.
Kim et al. (2004)TR6T cellsSimilarly, activated T cells did not release TR6.
Nguyen et al. (2001)CD45T cellsOn C2GnT(+) cells, CD45 and galectin-1 co-localized in patches on membrane blebs while no segregation of CD45 was seen on C2GnT(-) T cells, suggesting that oligosaccharide-mediated clustering of CD45 facilitated galectin-1-induced cell death.
Umetsu et al. (1988)IL-2T cellWe identified human T cell clones which secrete IL-4 but not IL-2 or IFN-gamma, and which appeared to correspond to murine Th2 clones.
Kelly-Rogers et al. (2006)CD56T cellsCD56+ T cells released interferon-gamma (IFN-gamma) and interleukin-13 (IL-13), but not IL-10, upon TCR stimulation.
Jaimes et al. (2002)CD4T cellsSignificantly higher frequencies of rotavirus-specific interferon gamma-secreting CD8(+) and CD4(+) T cells, but not IL-13-secreting T cells, were detected in symptomatically infected adults and exposed laboratory workers than in healthy adults and children with acute rotavirus diarrhea.
Manigold et al. (2010)IFNT cellsThis report as well as our data suggests that the vast majority of virus-specific T cells may not readily secrete IFN-?
Tjernlund et al. (2010)TatT cellsSpecifically, we did not detect any Tat SL8 (an epitope that is immundominant in early SIV infection) specific CD8+ T cells in our tissue sections, which is most likely due to the fact that these biopsies are taken from chronically infected rhesus macaques (77-85 days post-SIV infection), and the Tat SL8 response usually escapes during the acute infection phase [17].